Chinese Journal OF Rice Science ›› 2016, Vol. 30 ›› Issue (2): 152-160.DOI: 10.16819/j.1001-7216.2016.5113
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Yong-tao CUI, Li-wen WU, Shi-kai HU, De-yong REN, Chang-wei GE, Wei-jun YE, Guo-jun DONG, Long-biao GUO*(), Xing-ming HU*()
Received:
2015-07-13
Revised:
2015-10-18
Online:
2016-03-10
Published:
2016-03-10
Contact:
Long-biao GUO, Xing-ming HU
崔永涛, 吴立文, 胡时开, 任德勇, 葛常伟, 叶卫军, 董国军, 郭龙彪*(), 胡兴明*()
通讯作者:
郭龙彪,胡兴明
基金资助:
CLC Number:
Yong-tao CUI, Li-wen WU, Shi-kai HU, De-yong REN, Chang-wei GE, Wei-jun YE, Guo-jun DONG, Long-biao GUO, Xing-ming HU. Phenotypical Analysis and Fine Mapping of a Semi-dominant Dwarfism Gene Si-dd1 in Rice[J]. Chinese Journal OF Rice Science, 2016, 30(2): 152-160.
崔永涛, 吴立文, 胡时开, 任德勇, 葛常伟, 叶卫军, 董国军, 郭龙彪, 胡兴明. 水稻半显性矮秆基因Si-dd1的表型分析和精细定位[J]. 中国水稻科学, 2016, 30(2): 152-160.
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URL: http://www.ricesci.cn/EN/10.16819/j.1001-7216.2016.5113
材料 Material | 株高 Plant height /cm | 分蘖数 Tiller number | 一次枝梗 Number of primary rachis branches | 二次枝梗 Number of secondary rachis branches | 结实率 Seed-setting rate/% | 总粒数 Total grain number |
---|---|---|---|---|---|---|
野生型WT | 83.8 a | 18.7 | 8.0 | 9.0 a | 88.8 a | 72.7 a |
半矮秆Si-dd1(Aa) | 60.6 b | 16.7 | 8.0 | 16.3 b | 61.3 b | 90.7 b |
矮秆Si-dd1(AA) | 43.7 c | 16.3 | 9.6 | 16.3 b | 1.1 c | 97.3 b |
Table 1 Comparison of tiller, plant height and panicle traits among wild type, Si-dd1(Aa) and Si-dd1(AA).
材料 Material | 株高 Plant height /cm | 分蘖数 Tiller number | 一次枝梗 Number of primary rachis branches | 二次枝梗 Number of secondary rachis branches | 结实率 Seed-setting rate/% | 总粒数 Total grain number |
---|---|---|---|---|---|---|
野生型WT | 83.8 a | 18.7 | 8.0 | 9.0 a | 88.8 a | 72.7 a |
半矮秆Si-dd1(Aa) | 60.6 b | 16.7 | 8.0 | 16.3 b | 61.3 b | 90.7 b |
矮秆Si-dd1(AA) | 43.7 c | 16.3 | 9.6 | 16.3 b | 1.1 c | 97.3 b |
Fig. 1. Phenotypes of Si-dd1 at maturation stage. A, Whole plant; B, Panicle; C, Internode; D, Comparison of every internode. From left to right,wild type, Si-dd1(Aa) and Si-dd1(AA) in turn.
组合 Cross | F1 | F2 | χ2(1:2:1) | |||
---|---|---|---|---|---|---|
总株数 Total of plants | 正常高秆 Normal plants | 半矮秆 Semi-dwarf | 矮秆 Dwarf | |||
Si-dd1(AA)/9311 | 半矮秆 Semi-dwarf | 184 | 48 | 94 | 42 | 0.478 |
Si-dd1(AA)/NJ06 | 半矮秆 Semi-dwarf | 243 | 62 | 122 | 59 | 0.078 |
NJ06/Si-dd1(AA) | 半矮秆 Semi-dwarf | 193 | 47 | 100 | 46 | 0.264 |
9311/Si-dd1(AA) | 半矮秆 Semi-dwarf | 290 | 72 | 150 | 68 | 0.455 |
Table 2 Genetice analysis of F2 population of Si-dd1(AA) / NJ06(9311).
组合 Cross | F1 | F2 | χ2(1:2:1) | |||
---|---|---|---|---|---|---|
总株数 Total of plants | 正常高秆 Normal plants | 半矮秆 Semi-dwarf | 矮秆 Dwarf | |||
Si-dd1(AA)/9311 | 半矮秆 Semi-dwarf | 184 | 48 | 94 | 42 | 0.478 |
Si-dd1(AA)/NJ06 | 半矮秆 Semi-dwarf | 243 | 62 | 122 | 59 | 0.078 |
NJ06/Si-dd1(AA) | 半矮秆 Semi-dwarf | 193 | 47 | 100 | 46 | 0.264 |
9311/Si-dd1(AA) | 半矮秆 Semi-dwarf | 290 | 72 | 150 | 68 | 0.455 |
组合 Cross | 野生型 Wild type | 半矮秆 Semi-dwarf | 总株数 Total | χ2(1:1) |
---|---|---|---|---|
Si-dd1(Aa)/ 9311 | 54 | 48 | 102 | 0.353 |
Si-dd1(Aa)/ NJ06 | 59 | 54 | 113 | 0.220 |
9311 /Si-dd1(Aa) | 52 | 45 | 97 | 0.505 |
NJ06 /Si-dd1(Aa) | 52 | 48 | 100 | 0.160 |
Table 3 Genetice analysis of F1 population of Si-dd1(Aa) /NJ06 and Si-dd1(Aa) /9311.
组合 Cross | 野生型 Wild type | 半矮秆 Semi-dwarf | 总株数 Total | χ2(1:1) |
---|---|---|---|---|
Si-dd1(Aa)/ 9311 | 54 | 48 | 102 | 0.353 |
Si-dd1(Aa)/ NJ06 | 59 | 54 | 113 | 0.220 |
9311 /Si-dd1(Aa) | 52 | 45 | 97 | 0.505 |
NJ06 /Si-dd1(Aa) | 52 | 48 | 100 | 0.160 |
引物名称 Primer name | 引物序列(5'-3') Forward primer(5'-3') | 引物序列(5'-3') Reverse primer(5'-3') |
---|---|---|
M1 | CAGTCTTGCTCCGTTTGTTG | CTGTGACTGACTTGGTCATAGG |
M2 | ATCGCAGCAATGCCTCGTG | TGCGTTTGTGTTTGGCTCG |
M3 | CTCAACGTTGACACCTCGTG | TCCTCCATCGAGCAGTATCA |
Table 4 Makers for primary mapping of Si-DD1.
引物名称 Primer name | 引物序列(5'-3') Forward primer(5'-3') | 引物序列(5'-3') Reverse primer(5'-3') |
---|---|---|
M1 | CAGTCTTGCTCCGTTTGTTG | CTGTGACTGACTTGGTCATAGG |
M2 | ATCGCAGCAATGCCTCGTG | TGCGTTTGTGTTTGGCTCG |
M3 | CTCAACGTTGACACCTCGTG | TCCTCCATCGAGCAGTATCA |
引物名称 Primer name | 引物序列(5'-3') Forward primer(5'-3') | 引物序列(5'-3') Reverse primer(5'-3') |
---|---|---|
P1 | GGTCGCAGCTTGAATTAATGA | GCAATCTCATTTGTTGAGAACC |
P2 | TGATGTTTGGCACATACTTGC | GCAAACTTTCTGATAAGGAATAG |
P3 | CTCCAAAGCTGACAATGGTG | TGAGAAGGAGTAGGAAGCATAACA |
P4 | GGTACTAACCATGTGATTGAG | CACCTGAATTACCGTATATG |
P5 | CGTAGGAGTCGACGCTGTC | CCCAATCCGCTGTGGTTTT |
P6 | GCAGGTTGTAATGGAGGTGAA | CGGCGAGCCATATTGTTTAT |
Table 5 Makers for fine mapping of Si-DD1.
引物名称 Primer name | 引物序列(5'-3') Forward primer(5'-3') | 引物序列(5'-3') Reverse primer(5'-3') |
---|---|---|
P1 | GGTCGCAGCTTGAATTAATGA | GCAATCTCATTTGTTGAGAACC |
P2 | TGATGTTTGGCACATACTTGC | GCAAACTTTCTGATAAGGAATAG |
P3 | CTCCAAAGCTGACAATGGTG | TGAGAAGGAGTAGGAAGCATAACA |
P4 | GGTACTAACCATGTGATTGAG | CACCTGAATTACCGTATATG |
P5 | CGTAGGAGTCGACGCTGTC | CCCAATCCGCTGTGGTTTT |
P6 | GCAGGTTGTAATGGAGGTGAA | CGGCGAGCCATATTGTTTAT |
Fig. 3. Si-dd1 exposed to GA and BR. WT,Wild type; GA, Gibberellic acid; BR,Brassinolide. A, Plant phenotype after GA treatment at different concentrations; B, Plant height after GA treatment at different concentrations; C, Leaf angle after BR treatment at different concentrations.
Fig. 5. Comparison of microscopic structure among WT, Si-dd1(Aa) and Si-dd1(AA). A,B,C, Cross sections of leaf main vein of WT, Si-dd1(Aa) and Si-dd1(AA); D,E,F, Longitudinal section of the second internode from the top of WT, Si-dd1(Aa) and Si-dd1(AA); G,H,I, Cross sections of the second internode from the top of WT, Si-dd1(Aa) and Si-dd1(AA). J,K, Numbers of vascular bundles and longitudinal stem cells.
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