Response of Rice Organ Morphology and Dry Matter Accumulation to High Temperature at Different Panicle Initiation Stages

doi:10.16819/j.1001-7216.2016.5137

Abstract

Abstract:

A pot experiment in controlled-environment chambers was conducted to determine the effects of high temperature(40℃) on the rice organ morphology and dry matter accumulation during panicle initiation stage. Two mid-indica rice cultivars(Huanghuazhan,heat-tolerant;Fengliangyou 6,heat-sensitive) were planted and exposed to high temperature(40℃,10:00-15:00), and normal temperature(32℃,10:00-15:00) during branch-spikelet differentiation stage(I) and pollen mother cell formation-meiosis stage(Ⅱ), meanwhile rice was grown in ambient conditions as CK. The results indicated that, 1) at stage I high temperature inhibited panicle initiation, extending the panicle initiation stage by 2.5-8.8 d, while at stage Ⅱ,by 6.8-7.1d, which shortened the length of the top internode and reduced the heading degree,especially in Fengliangyou 6. 2)Heat stress at panicle initiation stage significantly decreased the number and the size of spikelets, which might be due to the significant difference in treatment period.High temperature at stage I inhibited spikelet differentiation, while at stageⅡ, heat stress mainly promoted spikelet degeneration,the degeneration rate of the two cultivars were up to 50%, with‘Fengliangyou6’ having a higher ratio. High temperature exposure at stage Ⅱ also significantly declined the size of anther, and the spikelet fertility. 3)High temperature promoted the growth of the top three leaves, especially at stage Ⅱ. Heat stress at the two stages had no significant effect on the leaf photosynthesis and dry matter accumulation of single main stem. Heat stress(40℃) exerted no significant influence on dry matter accumulation of culm, leaves and panicle at stage I, but significantly decreased the dry matter accumulation of culm and panicle at stage Ⅱ, leading to upper-internode tillering and resultant dry matter accumulation.

Key words: rice, panicle initiation stage, heat stress, organ morphology, dry matter accumulation

摘要：

为明确水稻穗分化期高温对生长发育所造成的影响,以耐热籼稻黄华占和热敏感籼稻丰两优6号为材料,利用人工气候箱在穗分化期进行40℃(10:00-15:00)高温处理,以人工气候箱32℃适温处理为参考,室外环境温度为对照(CK),研究枝梗-颖花分化期(Ⅰ期)和花粉母细胞形成-减数分裂期(Ⅱ期)高温胁迫对水稻器官形态及干物质积累的影响。结果表明:1)Ⅰ期高温抑制幼穗分化,使穗分化期延长2.5~8.8 d;Ⅱ期高温阻碍水稻抽穗,导致穗分化期延长6.8~7.1 d,显著缩短上部茎节,降低抽穗度,其中热敏感品种降幅大于耐热品种;2)穗分化期高温处理显著降低每穗颖花数和颖花大小,颖花数下降原因在不同处理时期间存在差异,Ⅰ期高温减少颖花分化数;而Ⅱ期高温显著加速颖花退化,两个品种退化幅度均达50%以上,并显著降低了花药大小和颖花受精率,热敏感品种丰两优6号受高温影响要大于耐热品种;3)高温对叶片生长有促进作用,Ⅱ期高温处理表现尤为明显,两个时期高温处理对上3叶净光合速率和水稻单茎干物质积累没有显著影响。Ⅰ期高温不同部位间干物质积累无显著差异,而Ⅱ期高温显著降低茎鞘和穗部干物质积累,但高温导致高节位分枝发生,积累了一部分干物质,整体上单茎干物质积累量并没有显著下降。

关键词: 水稻, 穗分化期, 高温胁迫, 器官形态, 干物质积累

CLC Number:

Ya-liang WANG, Yu-ping ZHANG, De-feng ZHU, Jing XIANG, Hui-zhe CHEN, Yi-kai ZHANG. Response of Rice Organ Morphology and Dry Matter Accumulation to High Temperature at Different Panicle Initiation Stages[J]. Chinese Journal OF Rice Science, 2016, 30(2): 161-169.

王亚梁, 张玉屏, 朱德峰, 向镜, 陈惠哲, 张义凯. 水稻器官形态和干物质积累对穗分化不同时期高温的响应[J]. 中国水稻科学, 2016, 30(2): 161-169.

Figures/Tables 8

References 33

[1]	李勇, 杨晓光, 叶清, 等. 全球气候变暖对中国种植制度可能影响:Ⅸ.长江中下游地区单双季稻高低温灾害风险及其产量影响.中国农业科学,2013,46(19):3997-4006.
	Li Y, Yang X G, Ye Q, et al.The possible effects of global warming on cropping systems in china:Ⅸ. The risk of high and low temperature disasters for single and double rice and its impacts on rice yield in the Middle-lower Yangtze plain.Sci Agric Sin, 2013,46(19):3997-4006.(in Chinese with English abstract)
[2]	徐富贤, 张林, 熊洪, 等. 杂交中稻开花期高温对结实率影响及其与组合间库源结构和开花习性的关系.作物学报,2015, 41(6): 946-955.
	Xu F X, Zhan L, Xiong H, et al.Effects of high temperature during flowering period on seed setting rate and its relationship with sink to source ratios and flowering habit of mid-seasonhybrid rice.Acta Agron Sin, 2015, 41(6): 946-955.(in Chinese with English abstract)
[3]	谢晓金, 申双和, 李秉柏, 等. 抽穗期高温胁迫对水稻开花结实的影响.中国农业气象,2009,30(2):252-256.
	Xie X J, Shen S H, Li B B, et al.Influences of high temperature stress on blooming and seed setting of rice during heading stage.Chin J Agrometeorol, 2009,30(2):252-256. (in Chinese with English abstract)
[4]	周建霞, 张玉屏, 朱德峰, 等. 高温下水稻开花习性对受精率的影响. 中国水稻科学, 2014, 28(3): 297-303.
	Zhou J X, Zhang Y P, Zhu D F, et al.Influence of flowering characteristics on spikelet fertility under high temperature.Chin J Rice Sci, 2014, 28(3): 297-303. (in Chinese with English abstract)
[5]	盛婧, 陈留根, 朱普平, 等.不同水稻品种抽穗期对高温的响应及避热的调控措施. 江苏农业学报, 2006, 22(4): 325-330.
	Sheng J, Chen L G, Zhu P P, et al.Responses of rice varieties to high temperature at heading stage and methods for heat escape .Jiangsu J Agric Sci, 2006,22(4): 325-330. (in Chinese with English abstract)
[6]	胡声博, 张玉屏, 朱德峰, 等. 杂交水稻耐热性评价. 中国水稻科学, 2012, 26(6): 751-756.
	Hu S B, Zhang Y P, Zhu D F, et al.Evaluation of heat resistance in hybrid rice.Chin J Rice Sci, 2012, 26(6): 751-756. (in Chinese with English abstract)
[7]	Eszter H, Gabriella S, Tibor J.Induction of abiotic stress tolerance by salicylic acid signaling.J Plant Growth Reg, 2007(26): 290-300.
[8]	段骅, 俞正华, 徐云姬, 等. 灌溉方式对减轻水稻高温危害的作用. 作物学报, 2012, 38(1): 107-120.
	Duan H, Yu Z J,Xu Y J, et al.Role of irrigation patterns in reducing harms of high temperature to rice.Acta Agron Sin, 2012, 38(1):107-120. (in Chinese with English abstract)
[9]	段骅, 傅亮, 剧成欣, 等. 氮素穗肥对高温胁迫下水稻结实和稻米品质的影响. 中国水稻科学, 2013, 27(6): 591-602.
	Duan H, Fu L, Ju C X, et al.Effects of application of nitrogen as panicle-promoting fertilizer on seed setting and grain quality of rice under high temperature stress.Chin J Rice Sci, 2013, 27(6): 591-602. (in Chinese with English abstract)
[10]	唐恬, 金荣花, 彭相瑜,等. 2013年夏季我国南方区域性高温天气的极端性分析.气象, 2014, 40(10):1207-1215.
	Tang T, Jin L H, Peng X Y, et al.Analysis on extremely high temperature over southern china in summer 2013.Meteorol Mon, 2014, 40(10):1207-1215.(in Chinese with English abstract)
[11]	曹云英, 段骅, 杨建昌, 等. 减数分裂期高温胁迫对耐热性不同水稻品种产量的影响及其生理原因.作物学报, 2008, 34(12): 2134-2142.
	Cao Y Y, Duan H, Yang L N, et al.Effect of heat-stress during meiosis on grain yield of rice cultivars differing in heat-tolerance and its physiological mechanism.Acta Agrono Sin, 2008, 34(12): 2134-2142.(in Chinese with English abstract)
[12]	魏金连, 潘晓华, 邓强辉.不同生育阶段夜温升高对双季水稻产量的影响.应用生态学报,2010,21(2):331-337.
	Wei J L, Pan X H, Deng Q H.Effects of night time temperature increase at different growth stages on double season rice grain yield.Chin J Appl Ecol, 2010, 21(2): 331-337.(in Chinese with English abstract)
[13]	邓运,田小海,吴晨阳,等. 热害胁迫条件下水稻花药发育异常的早期特征.中国生态农业学报,2010,18(2):377-383.
	Deng Y, Tian X H, Wu C Y, et al.Early signs of heat stress-induced abnormal development of anther in rice.Chin J Eco-Agric, 2010, 18(2): 377-383.(in Chinese with English abstract)
[14]	石春林, 金之庆, 郑建初, 等. 减数分裂期高温对水稻颖花结实率影响的定量分析.作物学报, 2008, 34(4): 627-631.
	Shi C L, Jin Z Q, Zhen J C, et al.Quantitative analysis on the effects of high temperature at meiosis stageon seed-setting rate of rice florets.Acta Agron Sin, 2008, 34(4):627-631. (in Chinese with English abstract)
[15]	凌启鸿, 蔡建中, 苏祖芳. 叶龄余数在稻穗分化进程鉴定中的应用价值. 中国农业科学, 1980,(4):1-11.
	Ling Q H, Cai J Z, Su Z F.The practical value of using the “leaf index” and “leaf remainder” in determining the stages of panicle differentiation in rice plant.Sci Agric Sin, 1980,(4):1-11. (in Chinese with English abstract)
[16]	松岛省三. 稻作的理论与技术.庞城译.北京:农业出版社.1966:121-133.
	Matsushima S.Theory and Technology of Rice Cultivation. Pang C Trans.Beijing:Agriculture Press,1966: 121-133. (in Chinese)
[17]	张卫星, 朱德峰, 徐一成, 等. 不同水分条件下水稻籽粒形态及其与粒重的关系.作物学报,2008,34(10):1826-1835.
	Zhang W X, Zhu D F, Xu Y C, et al.Grain morphological traits measured based on vision detection technology and their relation to grain weight in rice under different water condition.Acta Agron Sin, 2008, 34(10):1826-1835.(in Chinese with English abstract)
[18]	崔读昌. 气候变暖对水稻生育期影响的情景分析.应用气象学报,1995,6(3):361-365.
	Cui D C.The scenario analysis of possible effect of warming climate on rice growth period.Quarterly J Appl Meteorol, 1995,6(3):361-365. (in Chinese with English abstract)
[19]	宁金花,申双和.气候变化对中国农业的影响.现代农业科技,2009,(12):251-255.
	Ning J H, Shen S H.Effects of climate change on agriculture in China.ModAgric Sci Technol, 2009,(12):251-255.(in Chinese with English abstract)
[20]	董文军, 邓艾兴, 张彬, 等.开放式昼夜不同增温对单季稻影响的试验研究. 生态学报, 2011,31(8):2169-2177.
	Dong W J, Deng A X, Zhang B, et al.An experimental study on the the effects of different diurnal warming regimes on single cropping rice with Free Air Temperature Increased ( FATI) facility.Acta Ecol Sin, 2011, 31(8):2169-2177.(in Chinese with English abstract)
[21]	杨弘远. 水稻生殖生物学.杭州:浙江大学出版社,2005:22-23.
	Yang H Y.Rice reproduction biology. Zhejiang: Zhejiang University Press, 2005:22-23. (in Chinese)
[22]	张玉屏, 朱德峰, 林贤青, 等.高温对水稻剑叶生长和气孔导度影响.江西农业大学学报2012, 34(1):1-4.
	Zhang Y P, Zhu D F, Lin X Q, et al.Effect of high temperature stress on leaf growthand stomatal conductance in rice.Acta Agric Univ Jiangxiensis, 2012,34(1):1-4.(in Chinese with English abstract)
[23]	Zhang C X, Fu G F, Yang Y J, et al.Heat stress effects are stronger on spikelets than on flag leaves in rice due to differences in dissipation capacity.J Agron Crop Sci, 2015:0931-2250.
[24]	官华忠. 水稻包穗的遗传研究. 福州:福建农林大学,2011.
	Guan H Z.Genetic studies on panicle enclosure in rice(Oryza sativa L.) . Fujiang: Fujian Agriculture and Forestry University,2011. (in Chinese with English abstract)
[25]	魏育明, 郑有良. 内源激素对小麦可育小花数的调控.四川农业大学学报,1998, 16(3): 289-293.
	Wei Y M, Zheng Y L.The regulation of phytohormone in determination of fertile floret number in wheat.J Sichuan Agric Univ,1998,16(3):289-293. (in Chinese with English abstract)
[26]	Sakata T, Oshino T, Miura S, et al.Auxins reverse plant male sterility caused by high temperatures.PNAS, 2010,107(19):8569-8574.
[27]	张桂莲, 张顺堂, 肖浪涛,等. 抽穗开花期高温胁迫对水稻花药、花粉粒及柱头生理特性的影响.中国水稻科学,2014,28(2):155-166.
	Zhang G L, Zhang S T, Xiao L T, et al.Effect of high temperature stress on physiological characteristics of anther, pollen and stigma of rice during heading-flowering stage.Chin J Rice Sci, 2014, 28(2):155-166.(in Chinese with English abstract)
[28]	凌启鸿, 张洪程, 苏祖芳, 等. 稻作新理论.北京:科学出版社.1994:68-72.
	Ling Q H, Zhang H C, Su Z F, Ling L.The new theory on rice technology. Beijing: Science press.1994:67-72.
[29]	Laza M R C, Sakai H, Cheng,W G, et al. Differential response of rice plants to high night temperatures imposed at varying developmental phases.Agric For Meteorol,2015(209):69-77.
[30]	上海植物生理研究所人工气候室.高温对早稻开花结实的影响及其防治:Ⅱ. 早稻开花期高温对开花结实的影响.植物学报, 1976,18(4): 323-329.
	Laboratory of Phytotron, Shanghai Institute of Plant Physioligy. The influence of high temperature on the flowering and fruiting of high temperature on the flowering and fruiting of early rice and its control: Ⅱ.The influence of high temperature on the flowering-fruiting of early rice during the flowering stage.Acta Bot Sin, 1976, 18(4): 323-329.(in Chinese with English abstract)
[31]	李倩. 昼夜高温下水稻根源激素响应特征及其与产量的关系.武汉:华中农业大学,2012.
	Li Q.Responses of hormones to high day and night temperatures and their relationships with yield.Wuhan:Huazhong agricultural university, 2012. (in Chinese with English abstract)
[32]	张彬, 郑建初, 黄山, 等. 抽穗期不同灌水深度下水稻群体与大气的温度差异.应用生态学报,2008,19(1):87-92.
	Zhang B, Zheng J C, Huang S, et al.Temperature differences of air-rice plant under different irrigated water depth at spiking stage.Chin J Appl Ecol, 2008,19(1):87-92.(in Chinese with English abstract)
[33]	符冠富, 张彩霞, 杨雪芹, 等. 水杨酸减轻高温抑制水稻颖花分化的作用机理研究.中国水稻科学,2015,29(6):637-647.
	Fu G F, Zhang C X, Yang X Q, et al.Action mechanism by which SA alleviates high temperature-induced inhibition to spikelet differentiation.Chin J Rice Sci, 2015,29(6):637-647.(in Chinese with English abstract)

时期 TP	处理温度 TT	黄华占 Huanghuazhan			丰两优6号Fengliangyou 6
时期 TP	处理温度 TT	抽穗度 HD/%		抽穗速率 HS/(cm·d^-1)	抽穗度 HD/%	抽穗速率 HS/(cm·d^-1)
	CK	96.6±4.9 a	6.7±1.8 a	100.0±0.0 a		7.8±0.8 a
I	40℃	99.9±0.5 a	5.4±0.5 a	100.0±0.0 a		7.9±2.1 a
	32℃	99.0±2.8 a	5.8±0.6 a	99.8±1.2 a		7.4±1.9 a
Ⅱ	40℃	60.8±10.1 b	2.2±0.8 b	46.8±11.6 b		1.9±0.9 b
	32℃	96.5±5.3 a	5.4±1.8 a	100.0±0.0 a		8.2±1.6 a

时期 TP	处理温度 TT	黄华占 Huanghuazhan			丰两优6号Fengliangyou 6
时期 TP	处理温度 TT	抽穗度 HD/%		抽穗速率 HS/(cm·d^-1)	抽穗度 HD/%	抽穗速率 HS/(cm·d^-1)
	CK	96.6±4.9 a	6.7±1.8 a	100.0±0.0 a		7.8±0.8 a
I	40℃	99.9±0.5 a	5.4±0.5 a	100.0±0.0 a		7.9±2.1 a
	32℃	99.0±2.8 a	5.8±0.6 a	99.8±1.2 a		7.4±1.9 a
Ⅱ	40℃	60.8±10.1 b	2.2±0.8 b	46.8±11.6 b		1.9±0.9 b
	32℃	96.5±5.3 a	5.4±1.8 a	100.0±0.0 a		8.2±1.6 a

品种与时期 Cultivar and TP	处理温度 TT	株高 Plant height	穗长 Panicle length	第5节间长 5th IL	第4节间长 4th IL	第3节间长 3rd IL	第2节间长 2nd IL	第1节间长 1st IL
黄华占 Huanghuazhan
	CK	86.8±1.8 a	25.0±1.1 a	34.3±2.4 a	13.9±0.5 b	7.5±0.2 b	3.9±0.6 b	2.2±0.3 a
Ⅰ	40℃	85.3±3.1 a	23.0±2.5 a	32.8±2.2 a	14.7±0.5 ab	7.5±1.0 b	4.6±0.9 ab	2.7±0.7 a
	32℃	90.8±1.6 a	24.8±0.5 a	35.1±1.0 a	15.5±0.4 a	7.5±0.8 b	5.4±0.3 a	2.5±0.5 a
Ⅱ	40℃	75.1±2.7 b	22.6±0.2 b	25.0±1.5 b	13.5±0.6 b	6.7±0.3 b	4.9±0.6 ab	2.3±0.1 a
	32℃	88.5±1.2 a	24.0±0.7 a	33.6±1.5 a	14.1±1.0 ab	9.6±0.3 a	5.0±1.0 a	2.6±1.2 a
丰两优6号 Fengliangyou 6
	CK	103.6±2.0 a	29.5±0.7 a	37.1±0.6 a	18.1±0.1 a	11.0±1.0 b	5.8±0.7 ab	2.2±0.4 a
Ⅰ	40℃	103.1±4.4 a	24.3±1.3 b	36.7±3.1 a	19.8±0.7 a	17.1±0.6 a	3.1±0.3 b	2.0±0.6 a
	32℃	102.7±3.8 a	23.5±0.4 b	33.9±3.7 a	18.6±0.9 a	16.3±0.6 a	7.7±2.0 a	2.7±0.4 a
Ⅱ	40℃	65.5±2.7 c	23.0±0.2 b	15.1±0.3 b	12.7±1.0 c	7.8±0.4 c	7.2±0.4 a	2.4±0.3 a
	32℃	91.6±0.8 b	25.7±1.4 b	32.8±0.9 a	14.8±0.6 b	12.1±0.7 b	5.0±0.2 ab	2.1±0.3 a

品种与时期 Cultivar and TP	处理温度 TT	株高 Plant height	穗长 Panicle length	第5节间长 5th IL	第4节间长 4th IL	第3节间长 3rd IL	第2节间长 2nd IL	第1节间长 1st IL
黄华占 Huanghuazhan
	CK	86.8±1.8 a	25.0±1.1 a	34.3±2.4 a	13.9±0.5 b	7.5±0.2 b	3.9±0.6 b	2.2±0.3 a
Ⅰ	40℃	85.3±3.1 a	23.0±2.5 a	32.8±2.2 a	14.7±0.5 ab	7.5±1.0 b	4.6±0.9 ab	2.7±0.7 a
	32℃	90.8±1.6 a	24.8±0.5 a	35.1±1.0 a	15.5±0.4 a	7.5±0.8 b	5.4±0.3 a	2.5±0.5 a
Ⅱ	40℃	75.1±2.7 b	22.6±0.2 b	25.0±1.5 b	13.5±0.6 b	6.7±0.3 b	4.9±0.6 ab	2.3±0.1 a
	32℃	88.5±1.2 a	24.0±0.7 a	33.6±1.5 a	14.1±1.0 ab	9.6±0.3 a	5.0±1.0 a	2.6±1.2 a
丰两优6号 Fengliangyou 6
	CK	103.6±2.0 a	29.5±0.7 a	37.1±0.6 a	18.1±0.1 a	11.0±1.0 b	5.8±0.7 ab	2.2±0.4 a
Ⅰ	40℃	103.1±4.4 a	24.3±1.3 b	36.7±3.1 a	19.8±0.7 a	17.1±0.6 a	3.1±0.3 b	2.0±0.6 a
	32℃	102.7±3.8 a	23.5±0.4 b	33.9±3.7 a	18.6±0.9 a	16.3±0.6 a	7.7±2.0 a	2.7±0.4 a
Ⅱ	40℃	65.5±2.7 c	23.0±0.2 b	15.1±0.3 b	12.7±1.0 c	7.8±0.4 c	7.2±0.4 a	2.4±0.3 a
	32℃	91.6±0.8 b	25.7±1.4 b	32.8±0.9 a	14.8±0.6 b	12.1±0.7 b	5.0±0.2 ab	2.1±0.3 a

品种和时期 Cultivar and TP	处理温度 TT	叶面积 Leaf area/cm²				净光合速率 Net photosynthetic rate/(μmol·m^-2s^-1)
品种和时期 Cultivar and TP	处理温度 TT	剑叶 Flag leaf		倒2叶 Second leaf	倒3叶 Third leaf	剑叶 Flag leaf	倒2叶 Second leaf	倒3叶 Third leaf
黄华占 Huanghuazhan
	CK	71.8±5.1 b	63.0±1.0 a	40.8±1.7 a	29.1±2.9 a		26.0±2.0 a	17.7±2.0 a
Ⅰ	40℃	73.7±3.9 b	69.7±4.6 a	41.6±3.4 a	28.7±0.3 a		23.7±1.2 ab	16.5±1.0 a
	32℃	68.3±2.3 b	64.3±1.1 a	36.1±1.8 a	26.1±2.1 a		19.2±1.9 b	15.0±2.1 a
Ⅱ	40℃	84.0±3.7 a	68.2±5.2 a	38.7±6.2 a	27.4±1.7 a		26.6±0.8 a	19.2±1.8 a
	32℃	72.9±2.7 b	67.8±7.9 a	43.2±3.6 a	26.9±0.6 a		20.8±1.2 b	16.7±2.0 a
丰两优6号 Fengliangyou 6
	CK	64.1±4.3 b	57.3±6.5 a	38.1±5.5 b	26.2±1.4 a		23.6±3.4 a	15.1±1.0 b
Ⅰ	40℃	65.2±5.8 b	66.9±4.5 a	44.4±4.5 a	26.3±0.4 a		25.0±1.7 a	20.6±1.7 a
	32℃	63.3±3.0 b	67.9±4.8 a	47.4±8.2 a	27.6±1.3 a		21.0±2.1 a	16.0±1.6 b
Ⅱ	40℃	78.7±2.9 a	81.9±2.0 a	49.5±2.0 a	24.5±1.0 a		22.1±1.3 a	14.2±1.0 b
	32℃	60.2±6.4 b	58.9±2.3 a	42.4±4.5 a	26.5±2.2 a		24.4±1.2 a	15.8±0.9 b