Genetic Analysis and Gene Mapping of an Albino Lethal Mutant in Rice

doi:10.16819/j.1001-7216.2016.5133

Abstract

Abstract:

An albino lethal mutant, temporarily termed as al14 (albino 14),was obtained from ⁶⁰Co γ-ray radiated mutant pool of japonica rice variety Nipponbare. The mutant showed albino phenotype from germination, and died after 3-leaf stage. Genetic analysis revealed that the mutant phenotype was controlled by a single recessive nuclear gene. The chlorophyll and carotenoid contents in al14 declined dramatically. Transmission electron microscopy examination showed there were no obvious chloroplasts except prolamellar bodies in al14. The expression level of most genes of photosystemⅠand photosystem Ⅱdecreased dramatically, but the ribosomal genes and RNA polymerase genes in chloroplast increased notably in al14 mutant. In al14,the expression amount of PsbN, a photosystem Ⅱ protein N gene, was 118.23 times as high as that in wild type. By genetic mapping with an F₂ population generated by crossing the heterozygous al14 plants with indica variety Huanghuazhan, the al14 locus was mapped within 40 kb on chromosome 6. So far there is no reported gene relative to leaf color, so it is a novel gene controlling chloroplast development.

Key words: rice, albino, gene mapping

摘要：

从粳稻品种日本晴经⁶⁰Co诱变的M₁代材料中发现了一个白化致死突变体,该突变体从萌芽后一直表现白化,3叶期后逐渐衰亡。遗传分析表明,该突变表型受一对隐性核基因控制,将该白化突变体暂定名为 al14。与野生型相比, al14突变体的叶绿素含量与类胡萝卜素含量显著降低。电子显微镜观察表明 al14突变体不能形成完整的叶绿体,只有原片层体结构。对叶绿体编码基因的表达分析发现,突变体中光系统Ⅰ和光系统Ⅱ基因表达明显下调,核糖体结构基因和质体编码的RNA聚合酶亚基基因表达明显上调,但是PsbN(photosystem Ⅱ protein N)却上调表达水平最高,达到118.23倍。利用 al14 突变体与黄华占杂交获得的F₂代分离群体进行基因定位,将该基因定位于水稻第6染色体上约40 kb的范围。目前,该范围内没有叶色相关基因的报道,可能为一新的调控叶绿体发育的基因。

关键词: 水稻, 白化致死, 基因定位

CLC Number:

Q755
S511.01

Zhi-zhan CHU, Xiao-lin LIU, Yuan-ling CHEN, Yao-guang LIU. Genetic Analysis and Gene Mapping of an Albino Lethal Mutant in Rice[J]. Chinese Journal OF Rice Science, 2016, 30(2): 136-142.

初志战, 刘小林, 陈远玲, 刘耀光. 一个水稻白化致死突变基因的精细定位和遗传研究[J]. 中国水稻科学, 2016, 30(2): 136-142.

Figures/Tables 7

References 29

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材料 Material	叶绿素a含量 Chlorophyll a content/(mg·g^-1)	叶绿素b含量 Chlorophyll b content/(mg·g^-1)	类胡萝卜素含量 Carotenoids content/(mg·g^-1)	叶绿素a/叶绿素b Chlorophyll a/ chlorophyll b
野生型Wild type	3.83±0.27	1.30±0.12	0.78±0.03	2.97±0.07
al14	0.34±0.03^**	0.46±0.08^**	0.09±0.01^**	0.76±0.06^**

材料 Material	叶绿素a含量 Chlorophyll a content/(mg·g^-1)	叶绿素b含量 Chlorophyll b content/(mg·g^-1)	类胡萝卜素含量 Carotenoids content/(mg·g^-1)	叶绿素a/叶绿素b Chlorophyll a/ chlorophyll b
野生型Wild type	3.83±0.27	1.30±0.12	0.78±0.03	2.97±0.07
al14	0.34±0.03^**	0.46±0.08^**	0.09±0.01^**	0.76±0.06^**

标记 Marker	正向引物 Forward primer(5'-3')	反向引物 Reverse primer(5'-3')
607075	TGAGGATGGTTTTGGATGGG	CAATAGGGCAAACCTCAAC
607212	GAGTGAGCACTTACATTCGGT	ATCCTCCCTACACAAGGAGT
607420	GTCATTGCCAGGTAGATC	CTTCGGGACCAAATACAC
607489	AAATGTCCATGTGTTTGCTTC	CATGTGTGGAATGTGGTTG
607511	GTACCATCATTCTCCTACTCAC	TTCTACCTGAACCTGGAGTTC
607551	AAACGAGATCAGCCACGGAC	CTAGTGGAAGAGGTGGCTAC
608015	GCGCTGTTGGATATCATG	AATTCCTAGGCCACACAC
608275	GTAGCATAGGTATCGGATCTG	AATGGACGAACTGACGTCATG

标记 Marker	正向引物 Forward primer(5'-3')	反向引物 Reverse primer(5'-3')
607075	TGAGGATGGTTTTGGATGGG	CAATAGGGCAAACCTCAAC
607212	GAGTGAGCACTTACATTCGGT	ATCCTCCCTACACAAGGAGT
607420	GTCATTGCCAGGTAGATC	CTTCGGGACCAAATACAC
607489	AAATGTCCATGTGTTTGCTTC	CATGTGTGGAATGTGGTTG
607511	GTACCATCATTCTCCTACTCAC	TTCTACCTGAACCTGGAGTTC
607551	AAACGAGATCAGCCACGGAC	CTAGTGGAAGAGGTGGCTAC
608015	GCGCTGTTGGATATCATG	AATTCCTAGGCCACACAC
608275	GTAGCATAGGTATCGGATCTG	AATGGACGAACTGACGTCATG

编号 No.	基因 Gene	注释 Description
1	LOC_Os06g13590	假定的PE-PGRS家族蛋白 PE-PGRS family protein, putative, expressed
2	LOC_Os06g13600	假定的热重复家族蛋白 HEAT repeat family protein, putative, expressed
3	LOC_Os06g13610	表达蛋白 Expressed protein
4	LOC_Os06g13620	表达蛋白 Expressed protein
5	LOC_Os06g13630	假定的转座子蛋白 Transposon protein, putative, mutator sub-class, expressed