Chinese Journal OF Rice Science ›› 2016, Vol. 30 ›› Issue (2): 136-142.DOI: 10.16819/j.1001-7216.2016.5133
• Orginal Article • Previous Articles Next Articles
Zhi-zhan CHU1, Xiao-lin LIU2, Yuan-ling CHEN1, Yao-guang LIU1,*()
Received:
2015-09-09
Revised:
2015-12-05
Online:
2016-03-10
Published:
2016-03-10
Contact:
Yao-guang LIU
通讯作者:
刘耀光
基金资助:
CLC Number:
Zhi-zhan CHU, Xiao-lin LIU, Yuan-ling CHEN, Yao-guang LIU. Genetic Analysis and Gene Mapping of an Albino Lethal Mutant in Rice[J]. Chinese Journal OF Rice Science, 2016, 30(2): 136-142.
初志战, 刘小林, 陈远玲, 刘耀光. 一个水稻白化致死突变基因的精细定位和遗传研究[J]. 中国水稻科学, 2016, 30(2): 136-142.
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URL: http://www.ricesci.cn/EN/10.16819/j.1001-7216.2016.5133
材料 Material | 叶绿素a含量 Chlorophyll a content/(mg·g-1) | 叶绿素b含量 Chlorophyll b content/(mg·g-1) | 类胡萝卜素含量 Carotenoids content/(mg·g-1) | 叶绿素a/叶绿素b Chlorophyll a/ chlorophyll b |
---|---|---|---|---|
野生型Wild type | 3.83±0.27 | 1.30±0.12 | 0.78±0.03 | 2.97±0.07 |
al14 | 0.34±0.03** | 0.46±0.08** | 0.09±0.01** | 0.76±0.06** |
Table 1 Photosynthetic pigments contents in al14 and its wild type.
材料 Material | 叶绿素a含量 Chlorophyll a content/(mg·g-1) | 叶绿素b含量 Chlorophyll b content/(mg·g-1) | 类胡萝卜素含量 Carotenoids content/(mg·g-1) | 叶绿素a/叶绿素b Chlorophyll a/ chlorophyll b |
---|---|---|---|---|
野生型Wild type | 3.83±0.27 | 1.30±0.12 | 0.78±0.03 | 2.97±0.07 |
al14 | 0.34±0.03** | 0.46±0.08** | 0.09±0.01** | 0.76±0.06** |
标记 Marker | 正向引物 Forward primer(5'-3') | 反向引物 Reverse primer(5'-3') |
---|---|---|
607075 | TGAGGATGGTTTTGGATGGG | CAATAGGGCAAACCTCAAC |
607212 | GAGTGAGCACTTACATTCGGT | ATCCTCCCTACACAAGGAGT |
607420 | GTCATTGCCAGGTAGATC | CTTCGGGACCAAATACAC |
607489 | AAATGTCCATGTGTTTGCTTC | CATGTGTGGAATGTGGTTG |
607511 | GTACCATCATTCTCCTACTCAC | TTCTACCTGAACCTGGAGTTC |
607551 | AAACGAGATCAGCCACGGAC | CTAGTGGAAGAGGTGGCTAC |
608015 | GCGCTGTTGGATATCATG | AATTCCTAGGCCACACAC |
608275 | GTAGCATAGGTATCGGATCTG | AATGGACGAACTGACGTCATG |
Table 2 New InDel markers for mapping.
标记 Marker | 正向引物 Forward primer(5'-3') | 反向引物 Reverse primer(5'-3') |
---|---|---|
607075 | TGAGGATGGTTTTGGATGGG | CAATAGGGCAAACCTCAAC |
607212 | GAGTGAGCACTTACATTCGGT | ATCCTCCCTACACAAGGAGT |
607420 | GTCATTGCCAGGTAGATC | CTTCGGGACCAAATACAC |
607489 | AAATGTCCATGTGTTTGCTTC | CATGTGTGGAATGTGGTTG |
607511 | GTACCATCATTCTCCTACTCAC | TTCTACCTGAACCTGGAGTTC |
607551 | AAACGAGATCAGCCACGGAC | CTAGTGGAAGAGGTGGCTAC |
608015 | GCGCTGTTGGATATCATG | AATTCCTAGGCCACACAC |
608275 | GTAGCATAGGTATCGGATCTG | AATGGACGAACTGACGTCATG |
编号 No. | 基因 Gene | 注释 Description |
---|---|---|
1 | LOC_Os06g13590 | 假定的PE-PGRS家族蛋白 PE-PGRS family protein, putative, expressed |
2 | LOC_Os06g13600 | 假定的热重复家族蛋白 HEAT repeat family protein, putative, expressed |
3 | LOC_Os06g13610 | 表达蛋白 Expressed protein |
4 | LOC_Os06g13620 | 表达蛋白 Expressed protein |
5 | LOC_Os06g13630 | 假定的转座子蛋白 Transposon protein, putative, mutator sub-class, expressed |
Table 3 Gene annotated in mapping region.
编号 No. | 基因 Gene | 注释 Description |
---|---|---|
1 | LOC_Os06g13590 | 假定的PE-PGRS家族蛋白 PE-PGRS family protein, putative, expressed |
2 | LOC_Os06g13600 | 假定的热重复家族蛋白 HEAT repeat family protein, putative, expressed |
3 | LOC_Os06g13610 | 表达蛋白 Expressed protein |
4 | LOC_Os06g13620 | 表达蛋白 Expressed protein |
5 | LOC_Os06g13630 | 假定的转座子蛋白 Transposon protein, putative, mutator sub-class, expressed |
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