Chinese Journal OF Rice Science ›› 2015, Vol. 29 ›› Issue (2): 208-214.DOI: 10.3969/j.issn.1001-7216.2015.02.013
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Yang GAO1,2, Da-wei XUE2, Qian QIAN1, Zhen-yu GAO1,*()
Received:
2014-08-05
Revised:
2014-10-20
Online:
2015-03-10
Published:
2015-03-10
Contact:
Zhen-yu GAO
通讯作者:
高振宇
基金资助:
CLC Number:
Yang GAO, Da-wei XUE, Qian QIAN, Zhen-yu GAO. Application of the Second Generation Sequencing Technology in Rice Genomics and Transcriptomics[J]. Chinese Journal OF Rice Science, 2015, 29(2): 208-214.
高阳, 薛大伟, 钱前, 高振宇. 二代测序技术在水稻基因组学和转录组学研究中的应用[J]. 中国水稻科学, 2015, 29(2): 208-214.
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URL: http://www.ricesci.cn/EN/10.3969/j.issn.1001-7216.2015.02.013
测序对象 Sequencing objects | 测序仪器 Sequencing machines | 测序深度 Sequencing depth | 目的 Purpose | 文献 |
---|---|---|---|---|
敏感型IR64 耐旱型Nagina22 耐盐碱型Pokkali | Illumina Hiseq2000 | ≈10× | 挖掘对干旱和盐胁迫有差异反应的水稻 品种的遗传变异及其与功能的关系 | [21] |
水稻限制酶切片段 | Illumina GAⅡX | ≈19× | 证明简化基因组测序的可靠性和准确性 | [24] |
两优培九重组自交系 亲本93-11 亲本培矮64S | Illumina Hiseq2000 | ≈4× ≈36× ≈48× | 更新亲本基因组序列、QTL精细定位农艺 性状基因 | [25] |
40个栽培稻 10个野生稻 | Illumina GAⅡ | >15× | 鉴定重要农艺性状基因、揭示栽培稻驯化中的基因选择 | [27] |
3000份水稻品种 | Illumina Hiseq2000 | ≈14× | 构建水稻遗传数据库 | [28] |
1083个栽培稻 446个野生稻 | Illumina GAⅡX | ≈1× ≈2× | 水稻基因组多样性及栽培稻起源分析 | [32] |
澳洲普通野生稻 澳洲南方野生稻 | Illumina GAⅡX | 6.2× 4.9× | 研究水稻基因组的进化 | [33] |
淡绿叶突变体 | Illumina GAⅡX | ≤3× | 利用MutMap鉴定突变位点 | [38] |
517个地方品种 | Illumina GAⅡ | ≈1× | GWAS分析14个农艺性状位点 | [42] |
950个栽培品种 | Illumina GAⅡX | ≤1× | GWAS研究花期和粒重相关位点 | [43] |
日本晴生长7d的 苗和根 | Illumina GAⅡX | 定量水稻5号染色体着丝粒区域的 基因表达 | [46] | |
分蘖期和抽穗期的根 | Illumina Hiseq2000 | 研究杂种优势的分子机理 | [47,48] |
Table 1 The second generation sequencing application in rice research.
测序对象 Sequencing objects | 测序仪器 Sequencing machines | 测序深度 Sequencing depth | 目的 Purpose | 文献 |
---|---|---|---|---|
敏感型IR64 耐旱型Nagina22 耐盐碱型Pokkali | Illumina Hiseq2000 | ≈10× | 挖掘对干旱和盐胁迫有差异反应的水稻 品种的遗传变异及其与功能的关系 | [21] |
水稻限制酶切片段 | Illumina GAⅡX | ≈19× | 证明简化基因组测序的可靠性和准确性 | [24] |
两优培九重组自交系 亲本93-11 亲本培矮64S | Illumina Hiseq2000 | ≈4× ≈36× ≈48× | 更新亲本基因组序列、QTL精细定位农艺 性状基因 | [25] |
40个栽培稻 10个野生稻 | Illumina GAⅡ | >15× | 鉴定重要农艺性状基因、揭示栽培稻驯化中的基因选择 | [27] |
3000份水稻品种 | Illumina Hiseq2000 | ≈14× | 构建水稻遗传数据库 | [28] |
1083个栽培稻 446个野生稻 | Illumina GAⅡX | ≈1× ≈2× | 水稻基因组多样性及栽培稻起源分析 | [32] |
澳洲普通野生稻 澳洲南方野生稻 | Illumina GAⅡX | 6.2× 4.9× | 研究水稻基因组的进化 | [33] |
淡绿叶突变体 | Illumina GAⅡX | ≤3× | 利用MutMap鉴定突变位点 | [38] |
517个地方品种 | Illumina GAⅡ | ≈1× | GWAS分析14个农艺性状位点 | [42] |
950个栽培品种 | Illumina GAⅡX | ≤1× | GWAS研究花期和粒重相关位点 | [43] |
日本晴生长7d的 苗和根 | Illumina GAⅡX | 定量水稻5号染色体着丝粒区域的 基因表达 | [46] | |
分蘖期和抽穗期的根 | Illumina Hiseq2000 | 研究杂种优势的分子机理 | [47,48] |
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