Characterization and Gene Mapping of a No-pollen Genic Sterile Mutant whf41 in Rice

doi:10.16819/j.1001-7216.2017.6159 247

Abstract

Abstract:

【Objective】The anther cuticle and wax layer act as structural support and protective barriers for the development of pollen sac in rice. Phenotypic analysis and genetic mapping of the related genes can provide genetic resources and lay theoretical basis for further cloning and molecular mechanism analysis. 【Method】We identified a no-pollen male sterile mutant whf41 from the mutant progeny of ⁶⁰Co-γ-treated indica cultivar Zhonghui 8015. Anthers of the wild-type and whf41 mutant at different development stages were fixed for transverse section analysis and mature anthers of which were randomly selected for scanning electron microscopy analysis. BC₁F₁, F₁ lines and BC₁F₂, F₂ populations derived from whf41/ Zhonghui 8015 and whf41/ 02428 were used for genetic analysis and fine-mapping of the mutation site. 【Result】 Comparing with wild type,the whf41 mutant exhibited thin and transparent milky anther without mature pollen grains. Transverse section analysis showed that the whf41 mutant displayed aborted pollen grains,none detectable exine and swollen tapetal layer without expected PCD (programmed cell death), finally resulting in fragments of tapetal layer and pollen grains in the chamber. Scanning electron microscopy analysis further revealed the mutant had smoother anther wall, fewer lipids both on the inner and outer sides and fragments of degragated pollens. Genetic analysis revealed that whf41 was controlled by a single recessive genic gene, which was fine-mapped to a 45.6-kb region that harbored nine Open Reading Frames(ORFs) on the short arm of chromosome 3 between markers XD-5 and XD-11. Sequence analysis revealed that the whf41 mutant carried a nucleotide substitution and three nucleotide deletion, which led a substitution of one amino acid (D to M)and a deletion of one amino acid (V) in the fourth exon of one Cytochrome P450 family gene, LOC_Os03g0725, which is named as OsWHF41 and probably responsible for the no-pollen male sterility phenotype. Furthermore, qPCR analysis showed that the expression level of CYP704B2 and seven anther lipid synthesis and transportation related regulators changed significantly in whf41. 【Conclusion】Based on all these results, we deduced that the OsWHF41 gene was a new allelic to CYP704B2 gene and this further confirmed the important role of CYP704B2 in anther lipid synthesis and pollen exine formation .

Key words: rice, whf41, recessive genic male sterility, gene mapping

摘要：

【目的】水稻花药角质层和蜡质层是花粉囊发育重要的结构支撑和安全屏障。对花粉囊发育相关基因进行表型分析和遗传定位,为进一步克隆相关基因和分子机制研究提供基因资源和理论基础。【方法】从籼稻中恢8015辐射诱变(⁶⁰Co-γ)突变体库中分离鉴定出了一个无花粉型雄性不育突变体whf41。对野生型和突变体不同发育时期的花药进行半薄切片观察;并对其成熟期的花药进行扫描电镜观察。将突变体分别与中恢8015和02428杂交,构建BC₁F₁、F₁株系和BC₁F₂、F₂群体,对该表型进行遗传分析,采用图位克隆的方法精细定位目的基因。【结果】表型分析结果显示,whf41突变体的花药瘦小且呈透明乳白色,花药中不包含花粉粒细胞;半薄切片结果显示,突变体的小孢子无法形成正常的花粉外壁,绒毡层细胞异常膨大而不进行程序性死亡,最终膨胀的绒毡层和花粉细胞碎片逐渐融合并充满药室;扫描电镜结果进一步发现突变体花药内外壁均呈平滑状而缺乏脂类物质,花粉细胞逐渐破碎并降解。遗传分析表明,whf41突变体的无花粉型雄性不育性状受一对隐性核基因控制,我们将该基因精细定位于第3染色体短臂XD-5和XD-11两个标记之间,物理距离45.6 kb,其中包含9个开放阅读框。序列分析显示该区间内细胞色素P450基因LOC_Os03g07250的第4个外显子处存在1个单碱基替换和3个碱基的缺失,导致其翻译序列发生一个氨基酸的替换(天冬氨酸→甲硫氨酸)和一个氨基酸(缬氨酸)的缺失,致使其功能改变进而出现该表型。qRT-PCR检测结果表明,whf41突变体中CYP704B2和一系列花药脂质合成与转运相关基因的表达水平均发生了显著下调。【结论】根据本研究结果可推断,OsWHF41是CYP704B2的新等位基因,相关结果进一步明确CYP704B2在水稻花药脂质合成与花粉壁形成过程中的重要作用。

关键词: 水稻, whf41, 隐性核雄性不育, 基因定位

CLC Number:

Dandan XUAN, Lianping SUN, Peipei ZHANG, Yingxin ZHANG, Weixun WU, Zhengfu YANG, Xiaodeng ZHAN, Xihong SHEN, Liyong CAO, Shihua CHENG. Characterization and Gene Mapping of a No-pollen Genic Sterile Mutant whf41 in Rice[J]. Chinese Journal OF Rice Science, 2017, 31(3): 247-256.

轩丹丹, 孙廉平, 张沛沛, 张迎信, 吴玮勋, 杨正福, 占小登, 沈希宏, 曹立勇, 程式华. 水稻无花粉型核雄性不育突变体whf41的鉴定与基因定位[J]. 中国水稻科学, 2017, 31(3): 247-256.

Figures/Tables 7

Table 1 Primers used in the research.

引物名称 Primer name	上游引物 Forward primer(5'-3')	下游引物 Reverse primer(5'-3')
InD40	CTGCACCGGAGAAATTTGAT	CGCATGCAGATGAATAGGTG	精细定位 Fine maping
InD41	TAATTTCGGCTCATCCAAGC	GAAGCTCCGCAGGTTCAG	精细定位 Fine maping
RM14436	CTGACGCCGTCTTGCGTTATTCC	CGGCGACTTCGACTACTCAAGC	精细定位 Fine maping
RM14442	CGACACGGGCAAGAACTTATACGG	ATCCGATGACGGAGCATGATAGC	精细定位 Fine maping
XD-5	TGTCTTGTACCACTGCAATCAT	AACCCAAATCTAACAACTGACG	精细定位 Fine maping
XD-11	ACATAAATGATTGTTTCGTGGAG	CCTTTGAGTCAAAAAAAATAGGCA	精细定位 Fine maping
XD-12	CACTCAGTGGCAGATCCAAG	GTGCGTGAGTTCGTAAAGATGA	精细定位 Fine maping
GWHF41	GAGACGCTCCGCCTCTACC	ACTCCATCCTCCCCATGGA	突变位点检测 Mutation site detection
LOC_Os06g03930	CTGCTGTCCCAGTGGATGCTA	CCTCACCCCAAAGGTATGTCA	qRT-PCR
LOC_Os04g48460	CAAGTTCACGGCGTTCCAG	CGTCATCCCACATCTCGAATCTGAA	qRT-PCR
CYP704B2	AGCGGCAAGCAAGAGAAGA	GCCATCTTCATCTGGAGGTA	qRT-PCR
CYP703A3	TTTGATGCAGGACATGATT	TTGCCGGCGCTGAAGGGGA	qRT-PCR
GAMYB	CTCTCCAAAGTTTCCCCAGC	GAACATGATACCGTCGCCAA	qRT-PCR
WDA1	CAGATCATCCTGAGCGGTAT	CGAGTGGTAGTGGGTGTAGA	qRT-PCR
OSC4	GAGTGCGTGCCGCAGCTGAA	CTCTCGTCTCGTCTTAGGCAG	qRT-PCR
OSC6	TGCCACATCGTGAACCACACCCTG	ATGTTGAAATCCCTCCTTTGGTA	qRT-PCR

Fig. 1. Phenotypic comparison of wild type Zhonghui 8015(WT) and the whf41 mutant. A, Flower of wild type Zhonghui 8015 and the whf41 mutant with the lemma and palea removed; B, Anther of wild type and the whf41 mutant; C, Anther tablet of wild type and the whf41 mutant; D, I2-KI staining of pollen grains of wild type and the whf41 mutant.

Fig. 2. Transverse section of anthers of the wild type and whf41 at different developmental stages. A to E, Wild type; F to J, whf41 mutant; A and F, Cross-section of anthers at the stage 8; B and G, Cross-section of anthers at the stage 9; C and H, Cross-section of anthers at the stage 10; D and I, Cross-section of anthers at the stage 11; E and J, Cross-section of anthers at the stage 12. Ep, Epidermis; En, Endothecium; T, Tapetum; ST, Swollen tapetum; Tds, Tetrads; Msp, Microspores; DMs, Degenerated microspores; BP, Biceullar pollen; MP, Mature pollen. Bar=20 μm.

Fig. 3. SEM observation of the anther and pollen grains in wild type and the whf41 mutant. A, Anthers at stage 13 of the wild type and the mutant whf41, respectively; C, D and G, H, The outmost surface on epidermis of anthers at stage 13 of the wild type and whf41, respectively. E, F and I, J, The innermost surface on tapetum of anthers at stage 13 of the wild type and whf41, respectively. K and L, The pollen grain of anthers at stage 13 of the wild type and whf41. M, O and N, P, The pollen exine of anthers at stage 13 of the wild type and whf41, respectively. Bar= 20 mm in A and B, 5 mm in C to N, and 200 μm in O and P.

Table 2 Genetic analysis of the whf41 locus.

组合 Combination	F₁结实率 Seed-setting rate of F₁/%	F₂		χ²_(3:1)	χ²_0.05
组合 Combination	F₁结实率 Seed-setting rate of F₁/%	野生型植株数 No. of wild-type plants	突变表型植株数 No. of mutant-type plants	χ²_(3:1)	χ²_0.05
whf41/中恢8015 whf41/Zhonghui 8015	78.73	309	91	1.19	3.84
whf41/02428	80.51	3936	1278	0.63	3.84
中恢8015/02428 Zhonghui 8015/02428	85.98	6648	0	-	3.84

Fig. 4. Positional cloning of the whf41 mutated gene. A, Preliminary mapping of whf41; B, Fine mapping of whf41; C, The ORFs within the fine-mapped region; D, The structure candidate gene LOC_Os03g07250; E, The mutation site and translation sequence in whf41 mutant.

Fig. 5. Expression profile of the genes involved in anther development in wild type and whf41 plants. OsActin1 was used as an internal control. Error bars show the SD (n = 3). S8, S9, S11, and S12 indicated stage 8, stage 9, stage 11 and stage 12 of anther development; WT, Wild type.

References 39

[1]	朱英国. 水稻雄性不育生物学. 武汉: 武汉大学出版社, 2000: 1-2.
	Zhu Y G.Biology of Male Sterility in Rice. Wuhan: Wuhan University Press, 2000: 1-2. (in Chinese)
[2]	Raghavan V.Anther developmental biology in molecular embryology of flowering plants.Plant Physiol, 1997: 17-60.
[3]	Ma H.Molecular genetic analyses of microsporogenesis and microgametogenesis in flowering plants.Annu Rev Plant Boil, 2005, 56: 393-434.
[4]	Zhang D B, Wilson Z A.Stamen specification and anther development in rice. Chin Sci Bull, 2009, 54: 2342-2353.
[5]	Zhang D B, Luo X, Zhu L.Cytological analysis and genetic control of rice anther development.J Gen Genom, 2011, 38(9): 379-390.
[6]	Kinoshita T.Gene symbols and information on male sterility.Rice Genet Newsl, 1997, 14: 13-22.
[7]	Okamuro J K, Boer B G, Jofuku K D.Regulation of Arabidopsis flower development.Plant Cell, 1993, 5: 1183-1193.
[8]	马西青, 方才臣, 邓联武, 万向元. 水稻隐性核雄性不育研究进展及育种应用探讨.中国水稻科学,2012, 25(5): 511-520.
	Ma X Q, Fang C C, Deng L W, Wan X Y.Research progress and breeding application of recessive genic male sterility gene in rice.Chin J Rice Sci, 2012, 25(5): 511-520. (in Chinese with English abstract)
[9]	Nonomura K I, Miyoshi K, Eiguchi M, Suzuki T, Miyao A, Hirochika H, Kurata N.The MSP1 gene is necessary to restrict the number of cells entering into male and female sporogenesis and to initiate anther wall formation in rice.Plant Cell, 2003, 15(8): 1728-1739.
[10]	Nonomura K I, Nakano M, Fukuda T, Eiguchi M, Miyao A, Hirochika H, Kurata N.The novel gene HOMOLOGOUS PAIRING ABERRATION IN RICE MEIOSIS1 of rice encodes a putative coiled-coil protein required for homologous chromosome pairing in meiosis.Plant J, 2004, 16(4): 1008-1020.
[11]	Nonomura K I, Nakano M, Murata K, Miyoshi K, Eiguchi M, Miyao A, Hirochika H, Kurata N.An insertional mutation in the rice PAIR2 gene, the ortholog of Arabidopsis ASY1, results in a defect in homologous chromosome pairing during meiosis.Mol Gen Genom, 2004, 271(2): 121-129.
[12]	Yuan W Y, Li X W, Chang Y X, Wen R Y, Chen G X, Zhang Q F, Wu C.Mutation of the rice gene PAIR3 results in lack of bivalent formation in meiosis.Plant J, 2009, 59(2): 303-315.
[13]	Wang M, Wang K J, Tang D, Wei C X, Li M, Shen Y, Chi Z C, Gu M H, Cheng Z K.The central element protein ZEP1 of the synaptonemal complex regulates the number of crossovers during meiosis in rice.Plant Cell, 2010, 22: 417-430.
[14]	Nonomura K, Morohoshi A, Nakano M, Eiguchi M, Miyao A, Hirochika H, Kurata N.A germ cell-specific gene of the ARGONAUTE family is essential for the progression of premeiotic mitosis and meiosis during sporogenesis in rice.Plant Cell, 2007, 19(8): 2583-2594.
[15]	Wang C, Yue W, Ying Y, Wang S, Secco D, Liu Y, Whelan J, Tyerman S D, Shou H.Rice SPX-Major facility superfamily3, a vacuolar phosphate efflux transporter, is involved in maintaining phosphate homeostasis in rice.Plant Physiol, 2015, 169(4): 2822-2831.
[16]	He Y, Wang C, Higgins J D, Yu J, Zong J, Lu P, Zhang D, Liang W.MEIOTIC F-BOX is essential for male meiotic DNA double-strand break repair in rice.Plant Cell, 2016, 28(8): 1879-1893.
[17]	Li L, Li Y, Song S, Deng H, Li N, Fu X, Chen G, Yuan L.An anther development F-box (ADF) protein regulated by tapetum degeneration retardation (TDR) controls rice anther development.Planta, 2015, 241(1): 157-166.
[18]	Jung K H, Han M J, Lee Y S, Kim Y W, Hwang I, Kim M J, Kim Y K, Nahm B H, An G.Rice Undeveloped Tapetum1 is a major regulator of early tapetum development.Plant Cell, 2005, 17(10): 2705-2722.
[19]	Liu Z, Bao W, Liang W, Yin J, Zhang D.Identification of gamyb-4 and analysis of the regulatory role of GAMYB in rice anther development.J Integr Plant Biol, 2010, 52(7): 670-678.
[20]	Li H, Yuan Z, Vizcay-Barrena G, Yang C, Liang W, Zong J, Wilson Z A, Zhang D.PERSISTENT TAPETAL CELL1 encodes a PHD-Finger protein that is required for tapetal cell death and pollen development in rice.Plant Physiol, 2011, 156(2): 615-630.
[21]	Li X, Gao X, Wei Y, Deng L, Ouyang Y, Chen G, Li X, Zhang Q, Wu C.Rice APOPTOSIS INHIBITOR5 coupled with two DEAD-box adenosine 5′-triphosphate- dependent RNA helicases regulates tapetum degeneration.Plant Cell, 2011, 23(4): 1416-1434.
[22]	Niu N, Liang W, Yang X, Jin W, Wilson Z A, Hu J, Zhang D.EAT1 promotes tapetal cell death by regulating aspartic proteases during male reproductive development in rice. Nat Comm, 2013, 4: 1445.
[23]	Fu Z, Yu J, Cheng X, Zong X, Xu J, Chen M, Li Z, Zhang D, Liang W.The rice basic Helix-Loop-Helix transcription factor TDR INTERACTING PROTEIN2 is a central switch in early anther development.Plant Cell, 2014, 26(4): 1512-1524.
[24]	Niu B X, He F R, He M, Ren D, Chen L T, Liu Y G.The ATP-binding cassette transporter OsABCG15 is required for anther development and pollen fertility in rice.J Integr Plant Biol, 2013, 55(8): 710-720.
[25]	Zhao G, Shi J, Liang W, Xue F, Luo Q, Zhu L, Qu G, Chen M, Schreiber L, Zhang D.Two ATP binding cassette G transporters, rice ATP binding cassette G26 and ATP binding cassette G15, collaboratively regulate rice male reproduction.Plant Physiol, 2015, 169(3): 2064-2079.
[26]	Zhang D, Liang W, Yin C, Zong J, Gu F, Zhang D.OsC6, encoding a lipid transfer protein, is required for postmeiotic anther development in rice.Plant Physiol, 2010, 154(1): 149-162.
[27]	Jung K H, Han M J, Lee D Y, Lee Y S, Schreiber L, Franke R, Faust A, Yephremov A, Saedler H, Kim Y W, Hwang I, An G.Wax-Deficient Anther1 is involved in cuticle and wax production in rice anther walls and is required for pollen development.Plant Cell, 2006, 18(11): 3015-3032.
[28]	Shi J, Tan H, Yu X H, Liu Y, Liang W, Ranathunge K, Franke R B, Schreiber L, Wang Y, Kai G, Shanklin J, Ma H, Zhang D.Defective Pollen Wall is required for anther and microspore development in rice and encodes a fatty acyl carrier protein reductase.Plant Cell, 2011, 23(6): 2225-2246.
[29]	Xu D W, Shi J X, Rautengarten C, Yang L, Qian X L, Uzair M, Zhu L, Luo Q, An G, Waßmann F, Schreiber L, Heazlewood J L, Scheller H V, Hu J P, Zhang D B, Liang W Q.Defective Pollen Wall 2 (DPW2) encodes an acyl transferase required for rice pollen development.Plant Physiol, 2017, 173(1): 240-255.
[30]	Li H, Pinot F, Sauveplane V, Werck-Reichhart D, Diehl P, Schreiber L, Franke R, Zhang P, Chen L, Gao Y, Liang W, Zhang D.Cytochrome P450 family member CYP704B2 catalyzes the ω -hydroxylation of fatty acids and is required for anther cutin biosynthesis and pollen exine formation in rice. Plant Cell, 2010, 22(1): 173-190.
[31]	Yang X, Wu D, Shi J, He Y, Pinot F, Grausem B, Yin C, Zhu L, Chen M, Luo Z, Liang W, Zhang D.Rice CYP703A3, a cytochrome P450 hydroxylase, is essential for development of anther cuticle and pollen exine.J Integr Plant Biol, 2014, 56(10): 979-994.
[32]	Zhu Q H, Ramm K, Shivakkumar R, Dennis E S, Upadhyaya N M.The ANTHER INDEHISCENCE1 gene encoding a single MYB domain protein is involved in anther development in rice.Plant Physiol, 2004, 135(3): 1514-1525.
[33]	Kent B, Erin C, Nina D, David H, Young J K, Zhong Cathy X Y. Plant genomic DNA flanking SPT event and methods for identifying SPT event. United States Patent, US008257930B2, 20120904.
[34]	Chang Z Y, Chen Z F, Wang N, Xie G, Lu J W, Yan W, Zhou J L, Tang X Y, Deng X W.Construction of a male sterility system for hybrid rice breeding and seed production using a nuclear male sterility gene.Proc Natl Acad Sci USA, 2016, 113(49): 14145.
[35]	Sun L P, Zhang Y X, Zhang P P, Yang Z F, Zhan X D, Shen X H, Zhang Z H, Hu X, Xuan D D, Wu W X, Li Z H, Cao L Y, Cheng S H.K-Domain splicing factor OsMADS1 regulates open hull male sterility in rice.Rice Sci, 2015, 22(5): 207-216.
[36]	Orjuela J, Garavito A, Bouniol M, Arbelaez J D, Moreno L, Kimball J, Wilson G, Rami J F, Tohme J, McCouch S R, Lorieux M. A universal core genetic map for rice.Theor Appl Genet, 2010, 120: 563-572
[37]	Wu D H, Wu H P, Wang C S, Tseng H Y, Hwu K K.Genome-wide InDel marker system for application in rice breeding and mapping studies.Euphytica, 2013, 192: 131-143
[38]	Yamanaka S, Suzuki E, Tanaka M, Takeda Y, Watanabe J A, Watanabe K N.Assessment of cytochrome P450 sequences offers a useful tool for determining genetic diversity in higher plant species.Theor Appl Genet, 2003, 108(1): 1-9.
[39]	Tang W, Wu T, Ye J, Sun J, Jiang Y, Yu J, Tang J, Chen G, Wang C, Wan J.SNP-based analysis of genetic diversity reveals important alleles associated with seed size in rice.BMC Plant Biol, 2016, 16(1): 93.