Effects of Selenium Valence on Its Uptake and Translocation in Rice Under Drip Irrigation

doi:10.16819/j.1001-7216.2017.6069

Abstract

Abstract:

【Objective】 To lay a theoretical base for the development of selenium-enriched rice, a field plot experiment was performed to study the effects of selenate and selenite on selenium transformation and bioavailability under drip irrigation. 【Method】The experiment consisted of two factors: (i) amount of Se fertilizer (0.3, 0.6, and 1.2 kg/hm²), (ii) type of Se fertilizer (selenate and selenite). 【Result】The results indicated that selenate application at the level of 1.2 kg/hm² resulted in the highest soluble selenium concentration one day after fertilization (340% higher than control), 1.2 kg/hm² selenite application resulted in the highest exchangeable selenium, Fe oxide-bound selenium and organic matter-bound selenium concentrations four days after fertilization (380%, 56%, 59% higher than control). It could be seen that selenate mainly existed in soluble form in soil, while selenite could be transformed from the soluble form to other three forms, which decreased its bioavailability. We found that under selenite treatment selenium concentration in leaves and seeds was significantly lower than that under selenate treatment. However,under selenite treatment, the selenium content in root was significantly higher than that under selenate treatment. 【Conclusion】The selenium accumulations in shoot and root of rice were significantly higher under selenate treatments than those under the selenite treatments. Compared with the selenate treatment, absorption index, primary transport index and secondary transport index were lower in the selenite treatment. In conclusion, selenite has higher bioavailability than selenite in drip irrigation.

Key words: selenite, selenate, rice, bioavailability

摘要：

目的通过田间小区试验,研究外源硒酸盐及亚硒酸盐施入滴灌稻田中对土壤中不同价态硒含量及其生物有效性的影响,旨在为滴灌条件下富硒水稻的开发提供理论依据。方法以水稻品种T-43为材料,硒肥为亚硒酸盐与硒酸盐,施硒量为0.3、0.6、1.2 kg/hm²。结果研究结果表明,施肥后1 d硒酸盐1.2 kg/hm²处理土壤中可溶态硒含量最高,比对照高了340%;可交换态硒、铁氧化物态硒及有机态硒含量在施肥后4 d亚硒酸盐1.2 kg/hm²处理最高,较对照分别提高了380%、56%、59%,硒酸盐处理铁氧化物态硒及有机态硒含量增加不显著,说明硒酸盐主要以可溶态硒存在,而亚硒酸盐主要以可交换态硒存在,并且由可溶态转化为其他形态,因此降低了其有效性。对水稻的根、茎、叶及籽粒硒含量分析后发现,亚硒酸盐处理的籽粒和叶中的硒含量显著低于硒酸盐处理,而其根部硒含量显著大于硒酸盐处理,茎部硒含量无显著差异,且亚硒酸盐处理的根部硒累积量均大于硒酸盐处理。结论说明施用亚硒酸盐大部分积累在水稻根部,硒酸盐处理的地上部硒累积量大于亚硒酸盐处理,同时,硒酸盐处理的根系吸收系数、初级转运系数、次级转运系数均大于亚硒酸盐处理,说明亚硒酸盐大部分积累在水稻根部且向地上部转运较难,硒酸盐随水滴施的生物有效性高于亚硒酸盐。

关键词: 硒酸盐, 亚硒酸盐, 水稻, 生物有效性

CLC Number:

Dong ZHAND, Lichao SHI, Jun YE, Zhen’an HOU, Cunhu WANG, Yonghai WU. Effects of Selenium Valence on Its Uptake and Translocation in Rice Under Drip Irrigation[J]. Chinese Journal OF Rice Science, 2017, 31(1): 65-71.

张栋, 史力超, 冶军, 侯振安, 王存虎, 吴永海. 滴灌条件下不同价态外源硒对水稻硒吸收及转运的影响[J]. 中国水稻科学, 2017, 31(1): 65-71.

Figures/Tables 5

References 24

[1]	李莉萍, 王军. 土壤-植物系统中硒的赋存形态及其分析方法研究进展. 热带农业科学, 2009, 29(2): 58-66.
	Li L P, Wang J.Advances on existing form of analysis methods for selenium in soil-plant system.Chin J Trop Agric, 2009, 29(2): 58-66. (in Chinese with English abstract)
[2]	赵中秋, 郑海雷, 张春光, 马建华. 土壤硒及其与植物硒营养的关系. 生态学杂志, 2003, 22(1): 22-25.
	Zhao Z Q, Zheng H L, Zhang C G, Ma J H.Advance in the studies on selenium in soil and selenium biological effect.Chin J Ecol, 2003, 22(1): 22-25. (in Chinese with English abstract)
[3]	Sharma S, Bansal A, Dhillon S K, Dhillon K S.Comparative effects of selenate and selenite on growth and biochemical composition of rapeseed (Brassica napus L.). Plant Soil, 2010, 329(1-2) :339-348.
[4]	Cao Z H, Wang X C, Yao D H, Zhang X L, Wong M H.Selenium geochemistry of paddy soils in Yangtze River Delta.Environ Int, 2001, 26(6): 335-339.
[5]	Rayman M P.Food-chain selenium and human health: Emphasis on intake.Brit J Nutr, 2008, 100(2): 254-268.
[6]	Zhu Y G, Pilonsmits E A, Zhao F J, Williams P N, Meharg A A.Selenium in higher plants: Understanding mechanisms for biofortification and phytoremediation.Trends Plant Sci, 2009, 14(8): 436-442.
[7]	Miguel N A, Carmen C V.Selenium in food and the human body: A review.Sci Total Environ, 2008, 400(1-3): 115-141.
[8]	Haug A, Graham R D, Christophersen O A, Lyons G H.How to use the world's scarce selenium resources efficiently to increase the selenium concentration in food.Microb Ecol Health Dis, 2007, 19(4): 209-228.
[9]	Keskinen R, Turakainen M, Hartikainen H.Plant availability of soil selenate additions and selenium distribution within wheat and ryegrass.Plant Soil, 2010, 333(1): 301-313.
[10]	Harada T, Takahashi Y.Origin of the difference in the distribution behavior of tellurium and selenium in a soil-water system.Geochem Cosmo Chim Acta, 2008, 72(5): 1281-1294.
[11]	Banuelos G S, Lin Z Q.Phytoremediation management of selenium-laden drainageediments in the San Luis Drain: A greenhouse feasibility study.Ecotoxicol Environ Saf, 2005, 62(3): 309-316.
[12]	瞿建国,徐伯兴,龚书椿. 氢化物发生-无色散原子荧光光度法测定土壤中有效态硒和总硒. 土壤通报, 1998, 29(1): 47-53.
	Qu J G, Xu B X, Gong S C.Determination of bio-available and total selenium in soil by hydride generation non-dispersive AFS.Chin J Soil Sci, 1998, 29(1): 47-53. (in Chinese with English abstract)
[13]	Gangadhara, Kumar P R, Prakash V. The structure functional catalytic activity of rice brain lipase in the presence of selenium and lithium.Europ Food ResTechnol, 2010, 230(4): 551-557
[14]	周鑫斌, 施卫明, 杨林章. 水稻子粒硒累积机制研究. 植物营养与肥料学报, 2008, 14(3): 503-507.
	Zhou X B, Shi W M, Yang L Z.Study on mechanisms of seleniumaccumulation in rice grains.Plant Nutr Fert Sci, 2008, 14(3): 503-507. (in Chinese with English abstract)
[15]	王松山, 吴雄平, 梁东丽, 薛瑞玲,鲍俊丹. 不同价态外源硒在石灰性土壤中的形态转化及其生物有效性. 环境科学学报, 2010, 30(12): 2499-2505.
	Wang S S, Wu X P, Liang D L, Xue R L, Bao J D.Transformation and bioavailability for Pak choi (Brassica chinensis) of different forms of selenium added to calcareous soil. Acta Sci Circum, 2010, 30(12): 2499-2505. (in Chinese with English abstract)
[16]	于振, 李建科, 李梦颖,马倩倩, 黄瑞蕊, 何晓叶,李佳. 食品中微量硒测定方法研究进展. 食品工业科技, 2012, 33(18): 371-377.
	Yu Z, Li J K, Li M Y, Ma Q Q, Huang R R, He X Y, Li J.Research progress in determination of trace selenium in food.Sci Technol Food Ind, 2012, 33(18): 371-377. (in Chinese with English abstract)
[17]	姜超强, 沈嘉, 祖朝龙. 水稻对天然富硒土壤硒的吸收及转运. 应用生态学报, 2015, 26(3): 809-816.
	Jiang C Q, Sheng J, Zu C L.Selenium uptake and transport of rice under different Se-enriched natural soils.Chin J Appl Ecol, 2015, 26(3): 809-816. (in Chinese with English abstract)
[18]	王松山. 土壤中硒形态和价态及生物有效性研究. 杨陵:西北农林科技大学,2012.
	Wang S S.Fractionation and speciation of selenium in soil and its bioavailability.Yangling: Northwest Agriculture and Forestry University, 2012. (in Chinese with English abstract)
[19]	Fujita M, Ike M, Hashimoto R, Nakagawa T, Yamaguchi K, Soda S O.Characterizing kinetic of transport and transformation of selenium in water-sediment microcosm free from selenium contamination using a simple mathematical model.Chemosphere, 2005, 58(6): 705-714.
[20]	Munier-Lamy C, Deneux-Mustin S, Mustin C,Merlet D, Berthelin J, Leyval C.Selenium bioavailability and uptake as affected by four different plants in a loamy clay soil with particular attention to mycorrhizae inoculated ryegrass. JEnvir Radioac, 2007, 97(2-3): 148-158.
[21]	黄青青. 水稻和小麦对硒的吸收、转运及形态转化机制.北京:中国农业大学, 2015.
	Huang Q Q.Mechanisms of selenium uptake, translocation and speciation transformation in rice and wheat. Beijing: China Agric ultural University, 2015. (in Chinese with English abstract)
[22]	Sun G X,Liu X, Williams P N, Zhu Y G.Distribution and translocation of selenium from soil to grain and its speciation in paddy rice (Oryza sativa L). Environ Sci Technol, 2010, 44(17) : 6706-6711.
[23]	董广辉, 陈利军, 武志杰. 植物硒素营养及其机理研究进展. 应用生态学报, 2002,13(11): 1487-1490.
	Dong G H, Chen L J, Wu Z J.Research advances in plant selenium nutrition and its mechanism.Chin J Appl Ecol, 2002, 13(11): 1487-1490. (in Chinese with English abstract)
[24]	Li J, Liang D, Qin S, Feng P, Wu X.Effects of selenite andselenate application on growth and shoot selenium accumulation of pak choi (Brassica chinensis L.) during successive planting conditions. Environ Sci Poll Res, 2015, 22(14):11076-86.

处理 Treatment	施硒量 Selenium application level / (kg·hm^-2)	硒含量 Selenium content /(mg·kg^-1)
处理 Treatment	施硒量 Selenium application level / (kg·hm^-2)	根 Root	茎 Stem	叶 Leaf	籽粒 Grain
对照 CK	0	0.156±0.024 d	0.063±0.003 c	0.109±0.006 d	0.024±0.003 f
硒酸盐Selenate	0.3	0.279±0.003 c	0.083±0.004 c	0.189±0.015 b	0.078±0.005 c
	0.6	0.255±0.016 c	0.116±0.006 b	0.203±0.019 b	0.106±0.002 b
	1.2	0.345±0.026 b	0.221±0.003 a	0.301±0.003 a	0.162±0.009 a
亚硒酸盐Selenite	0.3	0.300±0.009 bc	0.067±0.011 c	0.097±0.006 d	0.046±0.002 e
	0.6	0.419±0.007 a	0.134±0.008 b	0.142±0.010 c	0.062±0.002 d
	1.2	0.460±0.025 a	0.205±0.019 a	0.143±0.006 c	0.076±0.001 c
两因素方差分析 (F值) Two-way ANOVA (F value)
不同价态硒(Se) Different valence of Se		101.60^*	0.88ⁿ	339.30^*	533.21^*
施硒量(C) Selenium content		195.81^*	324.16^*	130.20^*	515.84^*
Se×C		27.12^*	4.77ⁿ	61.25^*	103.87^*

处理 Treatment	施硒量 Selenium application level / (kg·hm^-2)	硒含量 Selenium content /(mg·kg^-1)
处理 Treatment	施硒量 Selenium application level / (kg·hm^-2)	根 Root	茎 Stem	叶 Leaf	籽粒 Grain
对照 CK	0	0.156±0.024 d	0.063±0.003 c	0.109±0.006 d	0.024±0.003 f
硒酸盐Selenate	0.3	0.279±0.003 c	0.083±0.004 c	0.189±0.015 b	0.078±0.005 c
	0.6	0.255±0.016 c	0.116±0.006 b	0.203±0.019 b	0.106±0.002 b
	1.2	0.345±0.026 b	0.221±0.003 a	0.301±0.003 a	0.162±0.009 a
亚硒酸盐Selenite	0.3	0.300±0.009 bc	0.067±0.011 c	0.097±0.006 d	0.046±0.002 e
	0.6	0.419±0.007 a	0.134±0.008 b	0.142±0.010 c	0.062±0.002 d
	1.2	0.460±0.025 a	0.205±0.019 a	0.143±0.006 c	0.076±0.001 c
两因素方差分析 (F值) Two-way ANOVA (F value)
不同价态硒(Se) Different valence of Se		101.60^*	0.88ⁿ	339.30^*	533.21^*
施硒量(C) Selenium content		195.81^*	324.16^*	130.20^*	515.84^*
Se×C		27.12^*	4.77ⁿ	61.25^*	103.87^*

处理 Treatment	吸收系数 Absorption index	初级转运系数 Primary transport index	次级转运系数 Secondary transport index
CK	0.581±0.009 d	0.457±0.003 c	0.350±0.007 c
S0.3	0.841±0.011 ab	0.487±0.008 c	0.575±0.006 b
S0.6	0.853±0.008 ab	0.600±0.010 b	0.693±0.004 a
S1.2	0.922±0.005 a	0.759±0.008 a	0.623±0.007 ab
Y0.3	0.717±0.003 c	0.256±0.008 e	0.317±0.006 c
Y0.6	0.800±0.009 bc	0.329±0.009 d	0.331±0.010 c
Y1.2	0.860±0.004 ab	0.365±0.008 d	0.373±0.009 c

处理 Treatment	吸收系数 Absorption index	初级转运系数 Primary transport index	次级转运系数 Secondary transport index
CK	0.581±0.009 d	0.457±0.003 c	0.350±0.007 c
S0.3	0.841±0.011 ab	0.487±0.008 c	0.575±0.006 b
S0.6	0.853±0.008 ab	0.600±0.010 b	0.693±0.004 a
S1.2	0.922±0.005 a	0.759±0.008 a	0.623±0.007 ab
Y0.3	0.717±0.003 c	0.256±0.008 e	0.317±0.006 c
Y0.6	0.800±0.009 bc	0.329±0.009 d	0.331±0.010 c
Y1.2	0.860±0.004 ab	0.365±0.008 d	0.373±0.009 c

处理 Treatment	单株成穗数 Panicle number per plant	每穗粒数 Grain numbers per panicle	千粒重 Thousand grain weight /g	产量 Yield/(kg·hm^-2)
CK	11.3±0.6 a	114.7±0.6 a	22.6±0.3 a	10 110±248.5 a
S0.3	11.3±0.6 a	116.3±0.6 a	22.4±0.6 a	10 179±233.8 a
S0.6	12.0±1.0 a	114.0±2.0 a	21.7±1.2 a	10 193±291.9 a
S1.2	11.3±0.6 a	117.0±1.0 a	22.4±0.2 a	10 202±242.0 a
Y0.3	11.3±0.6 a	116.0±1.0 a	22.3±1.0 a	10 084±241.2 a
Y0.6	11.3±0.6 a	114.3±1.2 a	22.9±0.2 a	10 201±312.4 a
Y1.2	11.7±0.6 a	116.3±1.5 a	21.8±0.1 a	10 200±338.0 a