Effects of Irrigation Regimes DuringGrain Filling Under Different Nitrogen Rates on Inferior SpikeletsGrain-Filling and Grain Yield of Rice

doi:10.16819/j.1001-7216.2018.7060

Abstract

Abstract:

【Objective】 To investigate the effects of water and nitrogen application on yield and grain filling,【Method】 an indica/japonica hybrid Yongyou 2640 with large-panicle and a japonica inbred Huaidao 5 with mid-panicle were grown in pots. After field seedling nursing then transplanting to pots, we designed three N rates, namely 0N (0 g N/pot), medium N level(MN, 2 g N/pot), and high N level(HN, 4 g N/pot), and three irrigation regimes post-anthesis consisting of conventional irrigation (CI, soil water potential was kept at 0 kPa), alternate wetting and moderate drying irrigation (WMD, rewatered when soil water potential reached -15 kPa), and alternate wetting and severe drying irrigation (WSD, rewatered when soil water potential reached -30 kPa). 【Result】 In the CI regime, MN showed the highest grain yield; in the WMD regimes, however, there was no significant difference in grain yield between MN and HN. Furthermore, in the WSD regime, grain yield under HN was the highest. In terms of grain filling, the superior spikelets present no significant difference in grain filling rate and final grain weight compared with all water-nitrogen treatments; Inferior spikeletsperforms better in grain filling rate and final grain weight at 0N and MN under CI and WMD regimes. However, in the WSD regime, 0N led to the lowest while HN showed the highest grain filling and final grain weight, but not significantly differentto MN. The above results showed that there was an obvious interaction between water and nitrogen. Among the varieties, grain filling rate and grain weight of inferior spikeletsof indica/japonica hybrid Yongyou 2640were lower than that ofjaponica inbred Huaidao 5, and the advantage of grain yield ofYongyou 2640 stemed from higher spikelet number per panicle. Finally, in the WMD+MN treatment, there was a higher nitrogen efficiency, creating higher grain yield with less nitrogen, and achieving the purpose of water and nitrogen saving. Secondly, it also eahanced the activity of root and leaves, improving the non-structural carbohydrate(NSC) remobilization, which promoted the upground biomass development and dry matter translocation in order to strengthen the inferior spikelets filling to induce an increase in grain yield. Therefore, it turns out to be the best water-nitrogen management in this research.

Key words: rice, yield, superior spikelets, inferior spikelets, interaction, physiological traits

摘要： 【目的】

旨在阐明氮肥和灌溉方式对水稻产量、籽粒灌浆及生理特性的影响。

【方法】

以大穗型品种甬优2640和中穗型品种淮稻5号为供试材料进行盆钵试验,大田育秧移栽后设置3种氮肥水平,即0N(不施氮)、MN(2g N/盆)、HN(4g N/盆);抽穗至成熟期设置3种灌溉方式,即CI(保持水层灌溉)、WMD(轻干湿交替灌溉, 土壤水势 -15 kPa时复水)、WSD(重干湿交替灌溉, 土壤水势 -30 kPa时复水)。

【结果】

在CI下,两个品种产量均以MN水平最高;WMD处理下,两个品种产量均以HN水平最高,但与MN下差异不显著,WSD处理下两个品种产量均以HN最高;而在籽粒灌浆上,两个品种强势粒的灌浆速率和最终粒重在各个水氮处理间无显著差异,弱势粒的灌浆速率和最终粒重在良好水势条件CI和轻度水分胁迫WMD下,分别在0 N和MN水平下表现较优;但在重度水分胁迫WSD下,0N水平表现最低,HN最高,但与MN差异不显著。以上都表明产量与弱势粒的灌浆在水氮间存在着明显的交互作用。在品种间,大穗型籼粳杂交稻甬优2640弱势粒灌浆速率及粒重都低于中穗型常规粳稻淮稻5号,其产量优势主要源自较高的每穗粒数。最后,WMD+MN处理下有较高的氮肥利用率,较少的施氮量获得较高的产量,达到节水节氮增产的效果,其次也增加了根系生理活性和叶片光合性能,非结构性碳水化合物(NSC)转运率,促进了地上部的生长发育,同时也加强了物质运转,促进了灌浆中后期弱势粒籽粒的充实,最终达到产量增加的目的,成为本研究最佳水氮运筹方式。

关键词: 水稻, 产量, 强势粒, 弱势粒, 互作, 生理特性

CLC Number:

Kuanyu ZHU, Mingfei ZHAN, CHENJing, Zhiqin WANG, Jianchang YANG, Buhong ZHAO. Effects of Irrigation Regimes DuringGrain Filling Under Different Nitrogen Rates on Inferior SpikeletsGrain-Filling and Grain Yield of Rice[J]. Chinese Journal OF Rice Science, 2018, 1(1): 155-168.

朱宽宇, 展明飞, 陈静, 王志琴, 杨建昌, 赵步洪. 不同氮肥水平下结实期灌溉方式对水稻弱势粒灌浆及产量的影响[J]. 中国水稻科学, 2018, 1(1): 155-168.

Figures/Tables 11

References 38

[1]	Kato T, Takeda K.Associations among characters related to yield sink capacity in space-planted rice.Crop Sci, 1996, 36: 1135-1139.
[2]	Kato T, Shinmura D, Taniguchi A.Activities of enzymes for sucrose-starch conversion in developing endosperm of rice and their association with grain filling in extra-heavy panicle types.Plant Prod Sci, 2007, 10: 442-450.
[3]	Peng S, Cassman K G, Virmani S S, Sheehy J, Khush G S.Yield potential trends of tropical since the release of IR8 and its challenge of increasing rice yield potential.Crop Sci, 1999, 39: 1552-1559.
[4]	Cheng S, Zhuang J, Fan Y, Du J, Cao L.Progress in research and development on hybrid rice: A super-domesticate in China.Ann Bot-London, 2007, 100: 959-966.
[5]	Peng S, Khush G S, Virk P, Tang Q, Zou Y.Progress in ideotype breeding to increase rice yield potential.Field Crop Res, 2008, 108: 32-38.
[6]	Mohapatra P K, Patel R, Sahu S K.Time of flowering affects grain quality and spikelet partitioning within the rice panicle.Aust J Plant Physiol, 1993, 20: 231-242.
[7]	Yang J C, Zhang J H.Grain-filling problem in ‘super’ rice.J Exp Bot, 2010, 61(1):1.
[8]	Wang Z, Zhang W, Beebout S S, Zhang H, Liu L, Yang J, Zhang J.Grain yield, water and nitrogen use efficiencies of rice as influenced by irrigation regimes and their interaction with nitrogen rates.Field Crop Res, 2016, 193:54-69.
[9]	张自常, 李鸿伟, 曹转勤, 王志琴, 杨建昌. 施氮量和灌溉方式的交互作用对水稻产量和品质的影响.作物学报,2013,39(1): 84-92.
	Zhang Z C, Li H W, Cao Z Q, Wang Z Q, Yang J C.Effect of interaction between nitrogen rate and irrigation regime on grain yield and quality of rice.ActaAgron Sin, 2013, 39(1):84-92.(in Chinese with English abstract)
[10]	Cabangon R J, Tuong T P, Castillo E G.Effect of irrigation method and N-fertilizer management on rice yield,water productivity and nutrient-use efficiencies in typical lowland rice conditions in China.Paddy＆Water Environ, 2004, 2:195-206.
[11]	王晓燕, 韦还和, 张洪程, 孙健, 张建民, 李超, 陆惠斌, 杨筠文, 马荣荣, 许久夫, 王珏, 许跃进, 孙玉海. 水稻甬优12 产量 13.5 t·hm-2以上超高产群体的生育特征. 作物学报, 2014, 40(12):2149-2159.
	Wang X Y, Wei H H, Zhang H C, Sun J, Zhang J M, Li C, Lu H B,Yang J W, Ma R R, Xu J F, Wang J, Xu Y J, Sun Y H.Population characteristics for super-high yielding hybrid rice Yongyou 12(>13.5 t ha-1).ActaAgron Sin, 2014, 40(12): 2149-2159.(in Chinese with English abstract)
[12]	姜元华, 张洪程, 赵可, 许俊伟, 韦还和, 龙厚元, 王文婷, 戴其根, 霍中洋, 许轲, 魏海燕, 郭保卫. 长江下游地区不同类型水稻品种产量及其构成因素特征的研究. 中国水稻科学,2014, 28(6): 621-631.
	Jiang Y H, Zhang H C, Zhao K, Xu J W, Wei H H, Long H Y,Wang W T, Dai Q G, Huo Z Y, Xu K, Wei H Y, Guo B W.Difference in yield and its components characteristics of different type rice cultivars in the lower reaches of the Yangtze River.Chin J Rice Sci, 2014, 28(6): 621-631.(in Chinese with English abstract)
[13]	Chen T, Xu Y, Wang J, Wang Z, Yang J, Zhang J.Polyamines and ethylene interact in rice grains in response to soil drying during grain filling.J ExpBot, 2013, 64(8):2523.
[14]	张伟杨, 徐云姬, 钱希旸, 李银银, 王志琴, 杨建昌. 小麦籽粒游离多胺对土壤干旱的响应及其与籽粒灌浆的关系. 作物学报, 2016, 42(6):860-872.
	Zhang W Y, Xu Y J, Qian X Y, Li Y Y, Wang Z Q, Yanng J C.Free Polyamines in Grains in Response to Soil Drought and Their Relationship with Grain Filling of Wheat.ActaAgron Sin, 2016, 42(6):860-872.(in Chinese with English abstract)
[15]	Yang J C, Zhang J H, Huang Z, Wang Z Q, Zhu Q S, Liu L J.Correlation of cytokinin levels in the endosperms and roots with cell number and cell division activity during endosperm development in rice.AnnBot-London,2002,90: 369-377.
[16]	Kato T.Change of sucrose synthase activity in developing endosperm of rice cultivars.Crop Sci, 1995, 35: 827-831.
[17]	Yoshida S, Forno D, Cock J, Gomez K.Determination of sugar and starch in plant tissue//Yoshida S. Laboratory Manual for Physiological Studies of Rice. Philippines: The International Rice Research Institute, 1976: 46-49.
[18]	王成瑷, 王伯伦, 张文香, 赵磊, 赵秀哲, 高连文. 土壤水分胁迫对水稻产量和品质的影响. 作物学报, 2006, 32(1): 131-137.
	Wang C Y, Wang B L, Zhang W X, Zhao L, Zhao X S, Gao L W.Effects of Water Stress of Soil on Rice Yield and Quality.ActaAgron Sin, 2006, 32(1): 131-137.(in Chinese with English abstract)
[19]	Tuong T P, Bouman B A M, Mortimer M. More rice, less water-integrated approaches for increasing water productivity in irrigated rice-based systems in Asia.Plant Prod Sci, 2005, 8: 231-241.
[20]	Yang J C, Liu K, Wang Z Q, Du Y, Zhang J H.Water-saving and high-yielding irrigation for lowland rice by controlling limiting values of soil water potential.J Integr Plant Biol, 2007, 49: 1445-1454.
[21]	蔡一霞,王维,朱智伟,张祖建,郎有忠,朱庆森. 结实期水分胁迫对不同氮肥水平下水稻产量及其品质的影响.应用生态学报, 2006, 17(7):1201-1206.
	Cai Y X, Wang W, Zhu Z W, Zhang Z J, Lang Y Z, Zhu Q S.Effects of water stress during grain-filling period on rice grain yield and its quality under different nitrogen levels.Chin J ApplEcol, 2006, 17(7):1201-1206.(in Chinese with English abstract)
[22]	魏海燕, 张洪程, 戴其根, 霍中洋, 许柯, 杭杰, 马群, 张胜飞, 张庆, 刘艳阳. 不同水稻氮利用效率基因型的物质生产与积累特性. 作物学报,2007(11): 1802-1809.
	Wei H Y, Zhang H C, Dai Q G, Huo Z Y, Xu K, Hang J, Ma Q, Zhang S F, Zhang Q, Liu Y Y.Characteristics of Matter Production and Accumulation in Rice Genotypes with Different N Use Efficiency.ActaAgron Sin, 2007(11): 1802-1809.(in Chinese with English abstract)
[23]	Jiao Z H, Hou A, Shi Y, Huang G H, Wang Y H, Chen X .Water management influencing methane and nitrous oxide emissions from rice field in relation to soil redox and microbial community.CommunSci Plant, 2006, 37(13-14): 1889-1903.
[24]	XueY, DuanH, LiuL, WangZ, YangJ, ZhangJ. An improved crop management increases grain yield and nitrogen and water use efficiency in rice.Crop Sci, 2013, 53: 271-284.
[25]	薛亚光,陈婷婷,杨成,王志琴,刘立军,杨建昌. 中粳稻不同栽培模式对产量及其生理特性的影响.作物学报, 2010, 36(3): 466-476.
	Xue Y G, Chen T T, Yang C, Wang Z Q, Liu L J, Yang J C.Effects of Different Cultivation Patterns on the Yield and Physiological Characteristics in Mid-Season Japonica Rice.ActaAgron Sin, 2010, 36(3): 466-476.(in Chinese with English abstract)
[26]	王绍华, 曹卫星, 丁艳锋, 田永超, 姜东. 水氮互作对水稻氮吸收与利用的影响.中国农业科学, 2004, 37(4):497-501.
	Wang S H, Cao W X, Ding Y F, Tian Y C, Jiang D.Interactions of Water Management and Nitrogen Fertilizer on Nitrogen Absorption and Utilization in Rice.SciAgric Sin, 2004, 37(4):497-501.(in Chinese with English abstract)
[27]	陈新红, 刘凯, 王志琴, 杨建昌. 水稻水氮互作效应与产量模型研究.西北农林科技大学学报: 自然科学版, 2006, 34(9): 141-148.
	Chen X H, Liu K, Wang Z Q, Yang J C.Studies on interactions between soil moisture and nitrogen and yield models in rice. J Northwest A&F Univ, 2006, 34(9): 141-148.(in Chinese with English abstract)
[28]	陈婷婷, 许更文, 钱希旸, 王志琴, 张耗, 杨建昌. 花后轻干-湿交替灌溉提高水稻籽粒淀粉合成相关基因的表达. 中国农业科学,2015, 48(7):1288-1299.
	Chen T T, Xu G W, Qian X Y, Wang Z Q, Zhang H, Yang J C.Post-anthesis alternate wetting and moderate soil drying irrigation enhance gene expressions of enzymes involved in starch synthesis in rice grains.SciAgric Sin, 2015, 48(7): 1288-1299.(in Chinese with English abstract)
[29]	徐云姬, 张伟杨, 钱希旸, 李银银, 张耗, 杨建昌.施氮量对小麦籽粒灌浆的影响及其生理机制. 麦类作物学报, 2015, 35(8): 1119-1126.
	Xu Y G, Zhang W Y, Qian X Y, Li Y Y, Zhang H, Yang J C.Effect of nitrogen on grain filling of wheat and its physiological mechanism.J Trit Crops, 2015, 35(8): 1119-1126.(in Chinese with English abstract)
[30]	Kato T, Sakurai N, Kuraishi S.The changes of endogenous abscisic acid in developing,grains of two rice cultivars with different grain size.Jpn J Crop Sci,1993, 62:456-461.
[31]	刘立军, 吴长付, 张耗, 杨建昌, 赵步洪. 实地氮肥管理对稻米品质的影响. 中国水稻科学, 2007, 21(6):625-630.
	Liu L J, Wu C F, Zhang H, Yang J C, Zhao B H.Effect of site-specific nitrogen management on rice quality. Chin J Rice Sci, 2007, 21(6): 625-630.(in Chinese with English abstract)
[32]	Yang J C, Zhang J H, Wang Z Q, Liu K, Wang P.Post-anthesis development of inferior and superior spikelets in rice in relation to abscisic acid and ethylene.J Exp Bot, 2006, 57: 149-160.
[33]	Yang J, Zhang J, Liu K, Wang Z, Liu L.Abscisic acid and ethyleneinteract in wheat grains in response to soil drying duringgrain filling. New Phytol, 2006, 271: 293-303.
[34]	Javid M G, Sorooshzadeh A, Sanavy. Effects of the exogenous application of auxin and cytokinin on carbohydrate accumulation in grains of rice under salt stress.Plant Growth Regul, 2011, 65(2): 305-313.
[35]	Saini H S, Westgate M E.Reproductive development in grain crops during drought.AdvAgron, 2000, 68: 59-96.
[36]	Inthapan P, Fukai S.Growth and yield of rice cultivars under sprinkler irrigation in south-eastern Queensland: 2. Comparison with maize and grain sorghum under wet and dry conditions.Austr J ExpAgric, 1988, 28:243-248.
[37]	Tao H, Brueck H, Ditten K, Kreye C, Lin S, Sattelmacher B. Growth and yield formation of rice (Oryza sativa L.) in the water-saving ground cover rice production system (GCRPS). Field Crop Res, 2006, 95(1): 1-12.
[38]	韦还和, 孟天瑶, 李超, 张洪程, 史天宇, 马荣荣, 王晓燕, 杨筠文, 戴其根, 霍中洋, 许轲, 魏海燕, 郭保卫. 籼粳交超级稻甬优538的穗部特征及籽粒灌浆特性. 作物学报, 2015, 41(12):1858-1869.
	Wei H H, Meng T Y, Li C, Zhang H C, Shi T Y, Ma R R, Wang X Y, Yang J W, Dai Q G, Huo Z Y, Xu K, Wei H Y, Guo B W.Panicle Traits and Grain-filling Characteristics of Japonica/Indica Hybrid Super Rice Yongyou 538.ActaAgron Sin, 2015, 41(12):1858-1869.(in Chinese with English abstract)

变异来源 Sourceofvariance	自由度df	产量 Yield	平均灌浆速率 Mean of grain filling rate	最终粒重 Final weight	NSC转运率 NSC remobiliza -tion rate	根系氧化力 Root oxidation	光合速率 Photosynthe -tic rate	蒸腾速率 Transpiration rate		根系IAA含量 IAA content of Root
年度Year(Y)	1	7.95^ns	8.58^ns	2.45^ns	3.32^ns	4.67^ns	4.77^ns	19.81^ns	16.35^ns		12.89^ns
品种Cultivar(C)	1	63.65^**	141.36^**	71.91^**	20.99^**	25.68^**	46.93^**	98.32^**	33.66^**		34.78^**
灌溉Irrigation(I)	2	18.98^**	148.92^**	89.15^**	28.22^**	86.82^**	48.90^**	484.03^**	14.15^**		16.52^**
氮肥Nitrogen(N)	2	15.09^**	24.66^*	54.88^**	11.62^**	53.53^*	54.91^*	5.66^*	40.68^**		42.26^**
Y×C	1	138.98^ns	148.92^ns	89.15^ns	202.22^ns	486.82^ns	486.90^ns	484.03^ns	12.15^ns		616.52^ns
Y×I	2	8.99^ns	6.08^ns	11.43^ns	102.62^ns	68.52^ns	57.14^ns	2.87^ns	59.37^ns		51.42^ns
Y×N	2	30.90^ns	11.82^ns	96.13^ns	22.54^ns	78.65^ns	33.64^ns	49.25^ns	25.45^ns		22.06^ns
C×I	2	99.57^**	0.35^ns	0.02^ns	0.95^ns	1.23^ns	1.22^ns	1.22^ns	64.53^**		64.56^**
C×N	2	152.52^**	0.21^ns	3.32^*	17.55^**	140.56^*	140.58^*	139.75^**	27.71^**		27.74^**
I×N	4	97.29^**	18.00^**	66.65^**	1.29^ns	0.51^ns	0.50^ns	0.50^ns	4.21^**		4.25^**
Y×C×I	2	2.68^ns	0.09^ns	0.93^ns	0.82^ns	0.05^ns	0.72^ns	0.01^ns	0.23^ns		0.21^ns
Y×C×N	2	3.62^ns	0.08^ns	0.97^ns	6.75^ns	5.23^ns	0.43^ns	0.83^ns	0.10^ns		0.09^ns
C×I×N	4	11.38^**	0.18^ns	2.05^ns	5.54^*	10.15^**	10.16^**	10.10^**	0.02^ns		0.02^ns
Y×I×N	4	2.11^ns	0.03^ns	0.90^ns	5.32^ns	44.24^ns	0.81^ns	0.11^ns	0.02^ns		0.01^ns
Y×C×I×N	4	3.92^ns	0.04^ns	0.92^ns	112.52^ns	68.93^ns	42.34^ns	60.52^ns		33.21^ns	8.97^ns

变异来源 Sourceofvariance	自由度df	产量 Yield	平均灌浆速率 Mean of grain filling rate	最终粒重 Final weight	NSC转运率 NSC remobiliza -tion rate	根系氧化力 Root oxidation	光合速率 Photosynthe -tic rate	蒸腾速率 Transpiration rate		根系IAA含量 IAA content of Root
年度Year(Y)	1	7.95^ns	8.58^ns	2.45^ns	3.32^ns	4.67^ns	4.77^ns	19.81^ns	16.35^ns		12.89^ns
品种Cultivar(C)	1	63.65^**	141.36^**	71.91^**	20.99^**	25.68^**	46.93^**	98.32^**	33.66^**		34.78^**
灌溉Irrigation(I)	2	18.98^**	148.92^**	89.15^**	28.22^**	86.82^**	48.90^**	484.03^**	14.15^**		16.52^**
氮肥Nitrogen(N)	2	15.09^**	24.66^*	54.88^**	11.62^**	53.53^*	54.91^*	5.66^*	40.68^**		42.26^**
Y×C	1	138.98^ns	148.92^ns	89.15^ns	202.22^ns	486.82^ns	486.90^ns	484.03^ns	12.15^ns		616.52^ns
Y×I	2	8.99^ns	6.08^ns	11.43^ns	102.62^ns	68.52^ns	57.14^ns	2.87^ns	59.37^ns		51.42^ns
Y×N	2	30.90^ns	11.82^ns	96.13^ns	22.54^ns	78.65^ns	33.64^ns	49.25^ns	25.45^ns		22.06^ns
C×I	2	99.57^**	0.35^ns	0.02^ns	0.95^ns	1.23^ns	1.22^ns	1.22^ns	64.53^**		64.56^**
C×N	2	152.52^**	0.21^ns	3.32^*	17.55^**	140.56^*	140.58^*	139.75^**	27.71^**		27.74^**
I×N	4	97.29^**	18.00^**	66.65^**	1.29^ns	0.51^ns	0.50^ns	0.50^ns	4.21^**		4.25^**
Y×C×I	2	2.68^ns	0.09^ns	0.93^ns	0.82^ns	0.05^ns	0.72^ns	0.01^ns	0.23^ns		0.21^ns
Y×C×N	2	3.62^ns	0.08^ns	0.97^ns	6.75^ns	5.23^ns	0.43^ns	0.83^ns	0.10^ns		0.09^ns
C×I×N	4	11.38^**	0.18^ns	2.05^ns	5.54^*	10.15^**	10.16^**	10.10^**	0.02^ns		0.02^ns
Y×I×N	4	2.11^ns	0.03^ns	0.90^ns	5.32^ns	44.24^ns	0.81^ns	0.11^ns	0.02^ns		0.01^ns
Y×C×I×N	4	3.92^ns	0.04^ns	0.92^ns	112.52^ns	68.93^ns	42.34^ns	60.52^ns		33.21^ns	8.97^ns

品种 Cultivar	处理 Treatment	每盆穗数 No.of panicle per pot	每穗粒数 Spikelet number per panicle	结实率 Seed setting rate /%	千粒重 1000-grain weight /g	产量 Grain yield /g·pot^-1
甬优2640 Yongyou 2640	CI+0N	11.2c	229.5c	90.5c	24.5b	57.0g
甬优2640 Yongyou 2640	CI+MN	18.7b	276.3b	89.5d	24.6b	114.3b
	CI+HN	21.2a	289.4a	75.9f	23.6c	110.2c
	WMD+0N	11.2c	230.1c	93.8a	25.5a	61.7f
	WMD+MN	18.6b	276.8b	93.2b	25.6a	122.7a
	WMD+HN	21.2a	289.2a	80.1e	25.4a	124.8a
	WSD+0N	11.4c	231.9c	74.7h	22.9e	44.7h
	WSD+MN	18.5b	274.9b	75.3g	23.4d	91.4e
	WSD+HN	21.1a	288.5a	75.5g	23.0d	105.7d
淮稻5号 Huaidao 5	CI+0N	13.8c	141.5c	91.9b	27.1b	48.6g
淮稻5号 Huaidao 5	CI+MN	23.0b	171.6b	91.7b	27.2b	97.0b
	CI+HN	24.2a	177.9a	80.7d	26.4c	91.7c
	WMD+0N	13.9c	141.9c	92.9a	27.7a	50.7f
	WMD+MN	22.8b	171.3b	92.5a	27.5a	99.9a
	WMD+HN	24.2a	177.0a	85.4c	27.6a	100.9a
	WSD+0N	13.7c	141.6c	80.1e	25.1e	39.0h
	WSD+MN	22.6b	169.8b	81.9d	25.8d	81.1e
	WSD+HN	24.0a	175.6a	82.2d	25.9d	89.7d

品种 Cultivar	处理 Treatment	每盆穗数 No.of panicle per pot	每穗粒数 Spikelet number per panicle	结实率 Seed setting rate /%	千粒重 1000-grain weight /g	产量 Grain yield /g·pot^-1
甬优2640 Yongyou 2640	CI+0N	11.2c	229.5c	90.5c	24.5b	57.0g
甬优2640 Yongyou 2640	CI+MN	18.7b	276.3b	89.5d	24.6b	114.3b
	CI+HN	21.2a	289.4a	75.9f	23.6c	110.2c
	WMD+0N	11.2c	230.1c	93.8a	25.5a	61.7f
	WMD+MN	18.6b	276.8b	93.2b	25.6a	122.7a
	WMD+HN	21.2a	289.2a	80.1e	25.4a	124.8a
	WSD+0N	11.4c	231.9c	74.7h	22.9e	44.7h
	WSD+MN	18.5b	274.9b	75.3g	23.4d	91.4e
	WSD+HN	21.1a	288.5a	75.5g	23.0d	105.7d
淮稻5号 Huaidao 5	CI+0N	13.8c	141.5c	91.9b	27.1b	48.6g
淮稻5号 Huaidao 5	CI+MN	23.0b	171.6b	91.7b	27.2b	97.0b
	CI+HN	24.2a	177.9a	80.7d	26.4c	91.7c
	WMD+0N	13.9c	141.9c	92.9a	27.7a	50.7f
	WMD+MN	22.8b	171.3b	92.5a	27.5a	99.9a
	WMD+HN	24.2a	177.0a	85.4c	27.6a	100.9a
	WSD+0N	13.7c	141.6c	80.1e	25.1e	39.0h
	WSD+MN	22.6b	169.8b	81.9d	25.8d	81.1e
	WSD+HN	24.0a	175.6a	82.2d	25.9d	89.7d

品种 Variety	处理 Treament	最大灌浆速率 G_max/(mg·grain^-1d^-1)		达最大灌浆速率时间 T_max/d		平均灌浆速率 G_mean/(mg·grain^-1d^-1)		活跃灌浆期 Active filling period/d		最终粒重A /(mg·grain^-1)
品种 Variety	处理 Treament	S	I	S	I	S	I	S	I	S	I
甬优2640	CI+0N	2.08a	1.16ab	14.37a	33.42f	1.49a	0.54b	18.43a	30.13d	27.46a	16.27c
Yongyou 2640	CI+MN	2.10a	1.13ab	14.42a	35.88e	1.48a	0.53b	18.52a	31.26c	27.41a	16.56c
	CI+HN	2.04a	1.02ab	14.83b	41.86b	1.46a	0.46c	18.55a	33.63a	27.08a	15.46d
	WMD+0N	2.08a	1.23a	14.38a	31.32g	1.50a	0.61a	18.30a	28.28e	27.45a	17.25b
	WMD+MN	2.10a	1.18ab	14.40a	32.12g	1.51a	0.62a	18.33a	30.12d	27.68a	18.67a
	WMD+HN	2.08a	1.15ab	14.50a	38.37d	1.47a	0.59a	18.46a	32.58b	27.14a	18.63a
	WSD+0N	2.05a	0.88c	14.95b	37.51d	1.47a	0.33e	18.35b	22.65g	26.97a	7.47f
	WSD+MN	2.11a	1.02b	14.45a	40.32c	1.48a	0.40d	18.39a	27.95f	27.22a	11.18e
	WSD+HN	2.09a	1.04b	14.52a	44.68a	1.46a	0.41cd	18.42a	28.12f	26.89a	11.52e
淮稻5号	CI+0N	2.26a	1.32ab	15.35a	26.56h	1.68a	0.80b	17.54a	24.26c	29.47a	19.40b
Huaidao 5	CI+MN	2.23a	1.27ab	15.38a	28.48e	1.70a	0.76b	17.56a	25.71b	29.85a	19.53b
	CI+HN	2.20b	1.2ab	15.37a	30.15b	1.69a	0.70c	17.65a	26.33a	29.83a	18.43c
	WMD+0N	2.28a	1.43a	15.38a	26.09i	1.70a	0.89a	17.52a	23.16d	29.78a	21.32a
	WMD+MN	2.25a	1.37ab	15.40a	27.31g	1.69a	0.91a	17.58a	24.21c	29.71a	22.03a
	WMD+HN	2.23a	1.24ab	15.42a	29.12d	1.67a	0.87a	17.68a	25.32b	29.53a	22.02a
	WSD+0N	2.22a	0.95c	15.33a	27.68f	1.71a	0.54e	17.42a	19.86f	29.79a	10.72e
	WSD+MN	2.24a	1.13b	15.41a	29.66c	1.70a	0.63d	17.54a	22.56e	29.82a	14.21d
	WSD+HN	2.19a	1.15b	15.42a	31.85a	1.69a	0.61d	17.52a	23.18d	29.61a	14.14d