Chinese Journal OF Rice Science ›› 2018, Vol. 32 ›› Issue (5): 427-436.DOI: 10.16819/j.1001-7216.2018.7037
• 研究论文 • Previous Articles Next Articles
Zhantian ZHANG, Yafei SUN, Hao AI, Wenzhen LUO, Bing FENG, Wenxian SUN, Guohua XU, Shubin SUN*()
Received:
2017-03-30
Revised:
2017-08-18
Online:
2018-09-10
Published:
2018-09-10
Contact:
Shubin SUN
张占田, 孙雅菲, 艾昊, 罗闻真, 冯冰, 孙文献, 徐国华, 孙淑斌*()
通讯作者:
孙淑斌
基金资助:
CLC Number:
Zhantian ZHANG, Yafei SUN, Hao AI, Wenzhen LUO, Bing FENG, Wenxian SUN, Guohua XU, Shubin SUN. Expression Patterns and Regulation of Transcription Factor Gene OsSHR2 in Vegetative Growth in Rice[J]. Chinese Journal OF Rice Science, 2018, 32(5): 427-436.
张占田, 孙雅菲, 艾昊, 罗闻真, 冯冰, 孙文献, 徐国华, 孙淑斌. 水稻转录因子基因OsSHR2的表达特征及其在营养生长中的调控作用[J]. 中国水稻科学, 2018, 32(5): 427-436.
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URL: http://www.ricesci.cn/EN/10.16819/j.1001-7216.2018.7037
Fig. 1. Bioinformatics analysis of SHR and SCR homologous genes of different species. A, Sequence homology analysis of amino acids and nucleic acid of SHR and SCR homologous genes in Arabidopsis thaliana and rice; B, Prediction of the positions of SHR and SCR homologous GRAS conserved domains and conserved motifs in Arabidopsis thaliana and rice by InterPro (http://www.ebi.ac.uk/interpro/); C, Phylogenetic tree analysis of SHR and SCR homologous genes of different species.
Fig. 3. Identification of OsSHR2 promoter-driven tissue-specific GUS staining. A, Seed root; B, Seed root and lateral root; C, Young leaf; D, The enlarged view of the young leaf; E, Leaf primordium; F, Ligule; G, Node; H, Stem and leaf sheath; I, Stem; J, Basal stem; K, Husk and rachilla; L, Spikelet; M, Ovary and stigma; N, Embryo(three days after germination). A-E, J: Seedling stage; F, G, I, K-N: Grain filling stage; H, Tilling stage. A-J, Bar=2 mm; K-N, Bar=0.5 mm.
Fig. 4. Phenotype and statistics of seed germination and hydroponics experiments of WT and osshr2. A, Germination phenotype(3, 5 and 8 days after germination), bar=5 cm; B, Hydroponics phenotype(14 days after germination), bar=5 cm; C, Shoot and root length (3, 5, 8 and 14 days after germination); DAG, Day after germination.
Fig. 5. Relative expression level of OsSHR1 and OsSHR2 under Pi-sufficient and Pi-deficient conditions in rice. A, The relative expression of OsSHR1 and OsSHR2 in the roots; B, The relative expression of OsSHR1 and OsSHR2 in the shoots.
Fig. 6. Phenotype of the wild type(WT) and osshr2 under Pi-sufficient and Pi-deficient conditions. A and B, Pi-deficient lasting three days; C and D, Pi-deficient lasting one week; E and F, Pi-deficient lasting two weeks; G, The phenotype of material under Pi-sufficient and Pi-deficient lasting two weeks, bar=20 cm. c410, osshr2-c410; c207, osshr2-c207.
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