Chinese Journal OF Rice Science ›› 2018, Vol. 32 ›› Issue (5): 437-444.DOI: 10.16819/j.1001-7216.2018.7154
• 研究论文 • Previous Articles Next Articles
Lingling DOU, Haichao HU, Long MA, Xiaonan KE, Mingyue LIU, Wangmin LIAN, Ke JIN, Lingjuan XIE, Qingpo LIU*()
Received:
2017-12-25
Revised:
2018-02-01
Online:
2018-09-10
Published:
2018-09-10
Contact:
Qingpo LIU
窦玲玲, 胡海超, 马龙, 柯笑楠, 刘明月, 练旺民, 金珂, 谢玲娟, 刘庆坡*()
通讯作者:
刘庆坡
基金资助:
CLC Number:
Lingling DOU, Haichao HU, Long MA, Xiaonan KE, Mingyue LIU, Wangmin LIAN, Ke JIN, Lingjuan XIE, Qingpo LIU. Functional Analysis of a Copper/Zinc SOD Encoding Gene in Response to Arsenite Stress in Rice[J]. Chinese Journal OF Rice Science, 2018, 32(5): 437-444.
窦玲玲, 胡海超, 马龙, 柯笑楠, 刘明月, 练旺民, 金珂, 谢玲娟, 刘庆坡. 一个水稻铜锌SOD酶基因在应答亚砷酸盐胁迫中的作用[J]. 中国水稻科学, 2018, 32(5): 437-444.
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URL: http://www.ricesci.cn/EN/10.16819/j.1001-7216.2018.7154
基因名称 Gene symbol | 正向引物 Forward primer(5′-3′) | 反向引物 Reverse primer(5′-3′) |
---|---|---|
Os08g44770.1 | GCTTCCACCTCCACGAGTTT | GCCACCCTTCCCCAAATCAT |
β-actin | TGTGGATTGCCAAGGCTGAG | ACGGCGATAACAGCTCCTC |
Table 1 The real-time RT-PCR primer sequences.
基因名称 Gene symbol | 正向引物 Forward primer(5′-3′) | 反向引物 Reverse primer(5′-3′) |
---|---|---|
Os08g44770.1 | GCTTCCACCTCCACGAGTTT | GCCACCCTTCCCCAAATCAT |
β-actin | TGTGGATTGCCAAGGCTGAG | ACGGCGATAACAGCTCCTC |
Fig. 1. PCR analysis of transgenic rice plants carrying overexpressed Os08g44770.1. M–DNA marker 2000; 1, Positive control; 2, 3, 5-7, 9-12, 14, 15, 19, 21, 23, 24 are transformed plants; The others are untransformed plants.
Fig. 4. Comparison of biomass of WT and transgenic rice plants after 6-day As(Ⅲ) treatment(n=8). Common lowercase letters above the bars indicate no significant difference among treatments or materials (P≤0.05).
Fig. 5. Comparison of relative root elongation of WT and transgenic rice plants under As(Ⅲ) stress for 6 days (n=8). Common lowercase letters above the bars indicate no significant difference among treatments or materials (P≤0.05).
处理 Treatment | 供试材料 Tested material | 叶绿素a含量 Chlorophyll a content / (mg∙g-1) | 叶绿素b含量 Chlorophyll b content / (mg∙g-1) | 总叶绿素含量 Chlorophyll a+b content / (mg∙g-1) | 类胡萝卜素含量 Carotenoid content / (mg∙g-1) | 叶绿素a /叶绿素b Chlorophyll a/ Chlorophyll b | 类胡萝卜素 /总叶绿素 Carotenoid/ Chlorophyll a+b |
---|---|---|---|---|---|---|---|
对照CK | WT | 24.25±1.16 a | 8.14±1.16 a | 32.39±1.79 a | 4.69±0.30 abc | 2.98±0.35 a | 0.14±0.02 b |
OE-44770-4 | 22.90±1.30 b | 6.20±1.30 ab | 29.21±2.68 ab | 4.94±0.25 ab | 3.67±0.50 a | 0.17±0.01 ab | |
OE-44770-13 | 23.90±1.34 a | 7.27±1.34 ab | 31.20±2.44 a | 5.20±0.07 a | 3.30±0.49 a | 0.17±0.01 ab | |
亚砷酸处理As(Ⅲ) stress | WT | 23.83±0.44 a | 7.03±0.20 ab | 30.86±0.59 a | 4.23±0.07 cd | 3.38±0.07 a | 0.14±0.00 b |
OE-44770-4 | 22.05±0.89 b | 5.41±0.07 b | 25.13±0.77 b | 3.78±0.07 d | 3.64±0.16 a | 0.15±0.00 ab | |
OE-44770-13 | 23.00±0.30 b | 5.33±0.16 b | 24.81±0.48 b | 4.50±0.29 bc | 3.65±0.17 a | 0.18±0.00 a |
Table 2 Effects of As(Ⅲ) stress on chlorophyll contents of wild type(WT) and transgenic rice plants (n=3).
处理 Treatment | 供试材料 Tested material | 叶绿素a含量 Chlorophyll a content / (mg∙g-1) | 叶绿素b含量 Chlorophyll b content / (mg∙g-1) | 总叶绿素含量 Chlorophyll a+b content / (mg∙g-1) | 类胡萝卜素含量 Carotenoid content / (mg∙g-1) | 叶绿素a /叶绿素b Chlorophyll a/ Chlorophyll b | 类胡萝卜素 /总叶绿素 Carotenoid/ Chlorophyll a+b |
---|---|---|---|---|---|---|---|
对照CK | WT | 24.25±1.16 a | 8.14±1.16 a | 32.39±1.79 a | 4.69±0.30 abc | 2.98±0.35 a | 0.14±0.02 b |
OE-44770-4 | 22.90±1.30 b | 6.20±1.30 ab | 29.21±2.68 ab | 4.94±0.25 ab | 3.67±0.50 a | 0.17±0.01 ab | |
OE-44770-13 | 23.90±1.34 a | 7.27±1.34 ab | 31.20±2.44 a | 5.20±0.07 a | 3.30±0.49 a | 0.17±0.01 ab | |
亚砷酸处理As(Ⅲ) stress | WT | 23.83±0.44 a | 7.03±0.20 ab | 30.86±0.59 a | 4.23±0.07 cd | 3.38±0.07 a | 0.14±0.00 b |
OE-44770-4 | 22.05±0.89 b | 5.41±0.07 b | 25.13±0.77 b | 3.78±0.07 d | 3.64±0.16 a | 0.15±0.00 ab | |
OE-44770-13 | 23.00±0.30 b | 5.33±0.16 b | 24.81±0.48 b | 4.50±0.29 bc | 3.65±0.17 a | 0.18±0.00 a |
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