Chinese Journal OF Rice Science ›› 2016, Vol. 30 ›› Issue (1): 27-34.DOI: 10.16819/j.1001-7216.2016.5101
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Chang-feng ZHU, Li-jun LIANG, Si-yuan ZENG, Tian-wei LI, Guan-shan DONG, De-lin HONG*()
Received:
2015-06-15
Revised:
2015-08-24
Online:
2016-01-10
Published:
2016-01-10
Contact:
De-lin HONG
朱长丰, 梁利君, 曾思远, 李天伟, 董冠杉, 洪德林*()
通讯作者:
洪德林
基金资助:
CLC Number:
Chang-feng ZHU, Li-jun LIANG, Si-yuan ZENG, Tian-wei LI, Guan-shan DONG, De-lin HONG. Fine Mapping of qFla-8-2 for Flag Leaf Angle in Rice[J]. Chinese Journal OF Rice Science, 2016, 30(1): 27-34.
朱长丰, 梁利君, 曾思远, 李天伟, 董冠杉, 洪德林. 水稻剑叶角度qFla-8-2位点的精细定位[J]. 中国水稻科学, 2016, 30(1): 27-34.
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URL: http://www.ricesci.cn/EN/10.16819/j.1001-7216.2016.5101
Fig. 1. Flag leaf angle of 863B and A7444 at different growth stages. A, Flag leaf angle of 863B (left) and A7444 (right) at the booting stage, bar=5 cm; B, Flag leaf angle of 863B (left) and A7444 (right) at the filling stage, bar=10 cm; C, Flag leaf angle of 863B (left), CF14-3 (middle, NIL of 863B) and A7444 (right) at the mature stage, bar=10 cm. Flag leaf angle was showed by the white arrows.
引物名称 Primer name | 正向引物(5'-3') Forward primer(5'-3') | 反向引物(5'-3') Reverse primer(5'-3') | 产物长度 Production length/bp |
---|---|---|---|
RM23021 | CTCAATGATGTTCTCGGCTTCC | GCCACAACGGTCAAGTAAAGAGC | 200 |
RM6215 | TTCAGCAGAGAGATGACGCAAGG | GTACAAACCAGCCCTCCGAGACG | 190 |
RM3153 | GTGTGATGGTGACGGATTACATGTGC | CATGCTGCAGAATTTCCATGTTGG | 402 |
RM3689 | CGTCAGCCGAAACTACTATCTAAACC | GTTTCACTGCACTCTGGTTTGC | 293 |
RM1309 | GAGGACACTGACGACAGCTTGG | CGCGCAAATCATTAAGTTCAGG | 186 |
RM3491 | GTGTTCTGATGTTCCCTCTCTGC | CCAACAAGGACTCACATGTCTCG | 120 |
RM8264 | TTCTACGGAATTTCTCCCTCTGG | CTAATCAATCTCTCGCGTTCTTGG | 162 |
RM8265 | TCGGCTGATCTGACCGTACATCC | GTGCATGCAACCACCTCTTGG | 185 |
RM5808 | GGGAGTAGGAGGGAGGGAGAAAGAGG | CAGAACCAGCGAGGGAAACACC | 112 |
Table 1 SSR primers used for further narrowing qFla-8 region.
引物名称 Primer name | 正向引物(5'-3') Forward primer(5'-3') | 反向引物(5'-3') Reverse primer(5'-3') | 产物长度 Production length/bp |
---|---|---|---|
RM23021 | CTCAATGATGTTCTCGGCTTCC | GCCACAACGGTCAAGTAAAGAGC | 200 |
RM6215 | TTCAGCAGAGAGATGACGCAAGG | GTACAAACCAGCCCTCCGAGACG | 190 |
RM3153 | GTGTGATGGTGACGGATTACATGTGC | CATGCTGCAGAATTTCCATGTTGG | 402 |
RM3689 | CGTCAGCCGAAACTACTATCTAAACC | GTTTCACTGCACTCTGGTTTGC | 293 |
RM1309 | GAGGACACTGACGACAGCTTGG | CGCGCAAATCATTAAGTTCAGG | 186 |
RM3491 | GTGTTCTGATGTTCCCTCTCTGC | CCAACAAGGACTCACATGTCTCG | 120 |
RM8264 | TTCTACGGAATTTCTCCCTCTGG | CTAATCAATCTCTCGCGTTCTTGG | 162 |
RM8265 | TCGGCTGATCTGACCGTACATCC | GTGCATGCAACCACCTCTTGG | 185 |
RM5808 | GGGAGTAGGAGGGAGGGAGAAAGAGG | CAGAACCAGCGAGGGAAACACC | 112 |
引物名称 Primer name | 正向引物(5'-3') Forward primer(5'-3') | 反向引物(5'-3') Reverse primer(5'-3') | 产物长度 Production length/bp |
---|---|---|---|
RM1309 | GAGGACACTGACGACAGCTTGG | CGCGCAAATCATTAAGTTCAGG | 189 |
RM23065 | CCACGAACTCTCCCTATATCTACTGC | CGTGCACACCTGAAGAGTATGG | 135 |
RM23071 | GTTCCGCCGTTGAGTGATGACC | TCCTCAGTCCTCCCTCTCCTTCC | 287 |
Z5 | GTCAAATCAGCTGGTTCAGTG | ATTTGACCTAACGATTGCGA | 200 |
Z7 | ATCCACGTCACCCACAACTC | CCCACGGAAAACCAAAACAT | 102 |
RM3491 | GTGTTCTGATGTTCCCTCTCTGC | CCAACAAGGACTCACATGTCTCG | 266 |
Table 2 SSR and InDel markers used for fine mapping of qFla-8-2.
引物名称 Primer name | 正向引物(5'-3') Forward primer(5'-3') | 反向引物(5'-3') Reverse primer(5'-3') | 产物长度 Production length/bp |
---|---|---|---|
RM1309 | GAGGACACTGACGACAGCTTGG | CGCGCAAATCATTAAGTTCAGG | 189 |
RM23065 | CCACGAACTCTCCCTATATCTACTGC | CGTGCACACCTGAAGAGTATGG | 135 |
RM23071 | GTTCCGCCGTTGAGTGATGACC | TCCTCAGTCCTCCCTCTCCTTCC | 287 |
Z5 | GTCAAATCAGCTGGTTCAGTG | ATTTGACCTAACGATTGCGA | 200 |
Z7 | ATCCACGTCACCCACAACTC | CCCACGGAAAACCAAAACAT | 102 |
RM3491 | GTGTTCTGATGTTCCCTCTCTGC | CCAACAAGGACTCACATGTCTCG | 266 |
Fig. 5. Map-based cloning of qFla-8-2. A, Fine mapping of qFla-8-2 genome region using mapping population with 438 plants. Numbers below the black horizontal line represent the numbers of recombinants between the marker and gene. B, qFla-8-2 was finally narrowed to a 67 kb DNA fragment. The thick black arrows mean predicted genes.
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