Chinese Journal OF Rice Science ›› 2015, Vol. 29 ›› Issue (5): 457-466.DOI: 10.3969/j.issn.1001G7216.2015.05.002
• Orginal Article • Previous Articles Next Articles
Lian-ping SUN1,2, Ying-xin ZHANG2, Pei-pei ZHANG2, Zheng-fu YANG2, Xiao-deng ZHAN2, Xi-hong SHEN2, Zhen-hua ZHANG2, Xia HU2, Dan-dan XUAN2, Wei-xun WU2, Li-yong CAO2,*(), Shi-hua CHENG1,2,*()
Received:
2015-02-04
Revised:
2015-05-06
Online:
2015-09-10
Published:
2015-09-10
Contact:
Li-yong CAO, Shi-hua CHENG
About author:
*Corresponding author:E-mailcaoliyong@cass.cn;shcheng@mail.hz.zj.cn
孙廉平1,2, 张迎信2, 张沛沛2, 杨正福2, 占小登2, 沈希宏2, 张振华2, 胡霞2, 轩丹丹2, 吴玮勋2, 曹立勇2,*(), 程式华1,2,*()
通讯作者:
曹立勇,程式华
作者简介:
*通讯录作者:E-mail:caoliyong@cass.cn;shcheng@mail.hz.zj.cn
基金资助:
Lian-ping SUN, Ying-xin ZHANG, Pei-pei ZHANG, Zheng-fu YANG, Xiao-deng ZHAN, Xi-hong SHEN, Zhen-hua ZHANG, Xia HU, Dan-dan XUAN, Wei-xun WU, Li-yong CAO, Shi-hua CHENG. Characterization and Gene Mapping of an Open Hull Male Sterile Mutant ohms1 Caused by Alternative Splicing in Rice[J]. Chinese Journal OF Rice Science, 2015, 29(5): 457-466.
孙廉平, 张迎信, 张沛沛, 杨正福, 占小登, 沈希宏, 张振华, 胡霞, 轩丹丹, 吴玮勋, 曹立勇, 程式华. 一个由可变剪接造成的水稻开颖不育突变体ohms1 的鉴定及基因定位[J]. 中国水稻科学, 2015, 29(5): 457-466.
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URL: http://www.ricesci.cn/EN/10.3969/j.issn.1001G7216.2015.05.002
引物名称 Primer name | 上游引物 Forward primer(5'-3') | 下游引物 Reverse primer(5'-3') | 实验目的 Purpose |
---|---|---|---|
InD44 | GGAATCCCTCCCTTCTTGTC | GGTCGGTAAAGACGGTGAAA | Fine mapping |
KY2 | GTGGGAAGAAGAACATCAACTG | GCACACAAGATAAACCCAATCAGC | Fine mapping |
KY12 | ACCACGAGGGTGACCGTAGA | GCGAGGGTTGATGAGATAGCA | Fine mapping |
KY17 | CGAGAGGCGAAGGAAATAGAACG | CTCCTCCTCCTCCTGGTTCTCC | Fine mapping |
KY25 | CCATGGTCGCCATTGACACG | CCTGCTATAACACTCGCACAGATGC | Fine mapping |
KY26 | GGTGGTGAGCCAAGAACTGACC | CCTCAAGGAATCCTCGTAAGTCG | Fine mapping |
KY29 | CCAAGTGTGTCCGAGCTTAGTGC | TGAGTCAAAGCGAAAGTCAACAGG | Fine mapping |
RM7576 | CTGCCCTGCCTTTTGTACAC | GCGAGCATTCTTTCTTCCAC | Fine mapping |
InD45 | CCAGGGATCTTCTCATCCAA | CCTGGCTAGCATACCACACA | Fine mapping |
InD46 | GCCATTGATCTTCTGCAGGT | TTTGTTGTCAATGCCCTGTT | Fine mapping |
RD0304 | GGCGTCACTGCTCGTA | GCCTGAAGCGTCCACA | Fine mapping |
CAPS1 | GCCATCGATCACCCTGAAAGTC | CTGATCAGCAAGAACAGTGC | ohms1 site detection |
CKY | AGCCAAACCACACCACCATAAAG | AGGACACTGTTTGCATTGGCT | cDNA sequencing |
Table 1 Primers used in this study.
引物名称 Primer name | 上游引物 Forward primer(5'-3') | 下游引物 Reverse primer(5'-3') | 实验目的 Purpose |
---|---|---|---|
InD44 | GGAATCCCTCCCTTCTTGTC | GGTCGGTAAAGACGGTGAAA | Fine mapping |
KY2 | GTGGGAAGAAGAACATCAACTG | GCACACAAGATAAACCCAATCAGC | Fine mapping |
KY12 | ACCACGAGGGTGACCGTAGA | GCGAGGGTTGATGAGATAGCA | Fine mapping |
KY17 | CGAGAGGCGAAGGAAATAGAACG | CTCCTCCTCCTCCTGGTTCTCC | Fine mapping |
KY25 | CCATGGTCGCCATTGACACG | CCTGCTATAACACTCGCACAGATGC | Fine mapping |
KY26 | GGTGGTGAGCCAAGAACTGACC | CCTCAAGGAATCCTCGTAAGTCG | Fine mapping |
KY29 | CCAAGTGTGTCCGAGCTTAGTGC | TGAGTCAAAGCGAAAGTCAACAGG | Fine mapping |
RM7576 | CTGCCCTGCCTTTTGTACAC | GCGAGCATTCTTTCTTCCAC | Fine mapping |
InD45 | CCAGGGATCTTCTCATCCAA | CCTGGCTAGCATACCACACA | Fine mapping |
InD46 | GCCATTGATCTTCTGCAGGT | TTTGTTGTCAATGCCCTGTT | Fine mapping |
RD0304 | GGCGTCACTGCTCGTA | GCCTGAAGCGTCCACA | Fine mapping |
CAPS1 | GCCATCGATCACCCTGAAAGTC | CTGATCAGCAAGAACAGTGC | ohms1 site detection |
CKY | AGCCAAACCACACCACCATAAAG | AGGACACTGTTTGCATTGGCT | cDNA sequencing |
组合 Combination | F1结实率 Seed-setting rate of F1/% | F2 | |||
---|---|---|---|---|---|
野生型植株数 No. of wild-type plants | 突变表型植株数 No. of mutant-type plants | ||||
ohms1/中花11 ohms1/Zhonghua 11 | 85.22 | 374 | 113 | 0.84 | 3.84 |
ohms1/02428 | 88.76 | 4658 | 1496 | 1.57 |
Table 2 Genetic analysis of the mutant ohms1.
组合 Combination | F1结实率 Seed-setting rate of F1/% | F2 | |||
---|---|---|---|---|---|
野生型植株数 No. of wild-type plants | 突变表型植株数 No. of mutant-type plants | ||||
ohms1/中花11 ohms1/Zhonghua 11 | 85.22 | 374 | 113 | 0.84 | 3.84 |
ohms1/02428 | 88.76 | 4658 | 1496 | 1.57 |
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