Chinese Journal OF Rice Science ›› 2015, Vol. 29 ›› Issue (2): 126-134.DOI: 10.3969/j.issn.1001-7216.2015.02.003
• Orginal Article • Previous Articles Next Articles
Xiao-jing LI1, Duo-duo XU1, Yi-min XU1, Kai-en ZHAI1, Yao-long YANG2, Jian-wei PAN1, Yu-chun RAO1,2,*()
Received:
2014-07-27
Revised:
2014-12-02
Online:
2015-03-10
Published:
2015-03-10
Contact:
Yu-chun RAO
李晓静1, 徐多多1, 徐益敏1, 翟开恩1, 杨窑龙2, 潘建伟1, 饶玉春1,2,*()
通讯作者:
饶玉春
基金资助:
CLC Number:
Xiao-jing LI, Duo-duo XU, Yi-min XU, Kai-en ZHAI, Yao-long YANG, Jian-wei PAN, Yu-chun RAO. Expression of OsBC88, a Rice Cellulose Synthase Catalytic Subunit Gene[J]. Chinese Journal OF Rice Science, 2015, 29(2): 126-134.
李晓静, 徐多多, 徐益敏, 翟开恩, 杨窑龙, 潘建伟, 饶玉春. 水稻纤维素合酶催化亚基的编码基因BC88的表达分析[J]. 中国水稻科学, 2015, 29(2): 126-134.
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URL: http://www.ricesci.cn/EN/10.3969/j.issn.1001-7216.2015.02.003
引物名称 Primer | 引物序列(5'-3') Primer sequence(5'-3') |
---|---|
pBC88-2035-HindⅢ-F | CCGAAGCTTCTTTTCCATTTTCATATTTCCCTGCCATCA |
pBC88-3896-BamHⅠ-R | GCCGGATCCGTGTGGGAGACGGTGGCGGGTTGGTTG |
BC88-no ATG-XbaⅠ-F | GGCTCTAGAGAGGCGAGCGCCGGGCTGGTGGCCGG |
BC88-stop codon-XbaⅠ-R | AGCTCTAGATCAGCAGTTGATGCCGCACTGCCTGACGTC |
BC88-600-R | CTCAGACACCGGGTAGGGGTGGATGC |
OsActin-Real-F | TGGCATCTCTCAGCACATTCC |
OsActin-Real-R | TGCACAATGGATGGGTCAGA |
OsBC-104-F | AGGTGTGCGAGATATGCGGCGACGAG |
OsBC-213-R | CTCGTACTCGTAGCAGGGGCGGCACAC |
Table 1 Primer sequences for OsBC88 cloning and Real-time PCR.
引物名称 Primer | 引物序列(5'-3') Primer sequence(5'-3') |
---|---|
pBC88-2035-HindⅢ-F | CCGAAGCTTCTTTTCCATTTTCATATTTCCCTGCCATCA |
pBC88-3896-BamHⅠ-R | GCCGGATCCGTGTGGGAGACGGTGGCGGGTTGGTTG |
BC88-no ATG-XbaⅠ-F | GGCTCTAGAGAGGCGAGCGCCGGGCTGGTGGCCGG |
BC88-stop codon-XbaⅠ-R | AGCTCTAGATCAGCAGTTGATGCCGCACTGCCTGACGTC |
BC88-600-R | CTCAGACACCGGGTAGGGGTGGATGC |
OsActin-Real-F | TGGCATCTCTCAGCACATTCC |
OsActin-Real-R | TGCACAATGGATGGGTCAGA |
OsBC-104-F | AGGTGTGCGAGATATGCGGCGACGAG |
OsBC-213-R | CTCGTACTCGTAGCAGGGGCGGCACAC |
Fig.1. Construction of PBI121 BC88 pro :: GUS vector. A, Structure of PBI121 BC88 pro :: GUS vector; B, PCR products of BC88 promoter; C, PCR identification of positive bacterial suspension; D, PCR identification of positive bacterial colony; M, DNA marker; CK, Positive control.
Fig. 2. Construction of pcambia 1300-2×35s:: eGFP: BC88 vector. A, Structure of pcambia 1300-2×35s:: eGFP: BC88 vector; B, PCR products of BC88; C, PCR identification of positive bacterial suspension; D, PCR identification of positive bacterial colony, M, DNA marker; CK, Positive control.
Fig. 3. Tissue expression analysis of OsBC88. A, GUS staining of root, culm, leaf, sheath of Nipponbare at seedling stage; B, GUS staining of flower of Nipponbare at flowering stage, serving as negative control; C, GUS staining of root, culm, sheath, leaf of pBI121: BC88 pro :: GUS transgenic lines at seedling stage; D, GUS staining of flower of pBI121:BC88 pro:: GUS transgenic lines at flowering stage.
Fig. 5. Subcellular localization of BC88. A, Localization of 35S:: eGFP and 35S:: eGFP: BC88 in tobacco epidermal cells; B, Localization of 35S:: eGFP in rice root tip cells, the bottom is the amplification of the upside. GFP indicates the green fluorescence of proteins, DIC indicates the differential interference contrast phase, and Merged indicates the merging of GFP and DIC.
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