Chinese Journal OF Rice Science ›› 2015, Vol. 29 ›› Issue (2): 126-134.DOI: 10.3969/j.issn.1001-7216.2015.02.003

• Orginal Article • Previous Articles     Next Articles

Expression of OsBC88, a Rice Cellulose Synthase Catalytic Subunit Gene

Xiao-jing LI1, Duo-duo XU1, Yi-min XU1, Kai-en ZHAI1, Yao-long YANG2, Jian-wei PAN1, Yu-chun RAO1,2,*()   

  1. 1 College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China
    2 State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China
  • Received:2014-07-27 Revised:2014-12-02 Online:2015-03-10 Published:2015-03-10
  • Contact: Yu-chun RAO

水稻纤维素合酶催化亚基的编码基因BC88的表达分析

李晓静1, 徐多多1, 徐益敏1, 翟开恩1, 杨窑龙2, 潘建伟1, 饶玉春1,2,*()   

  1. 1 浙江师范大学 化学与生命科学学院, 浙江 金华 321004
    2 中国水稻研究所 水稻生物学国家重点实验室, 杭州 310006
  • 通讯作者: 饶玉春
  • 基金资助:
    国家自然科学基金资助项目(31201183,31171520);中国博士后基金资助项目(2014M561108);浙江省重中之重学科开放基金资助项目

Abstract:

Brittle culm mutant in gramineae plants is an ideal material for secondary cell wall research. Previous studies about bc88 mutant in rice show that BC88 encodes a cellulose synthase catalytic subunit, OsCesA9. The genetic transformation of rice by BC88 promoter fused with GUS vector showed that BC88 was expressed ubiquitously in root, stem, leaf, sheath, flower, with high expression in culm and root. The mutation in BC88 may affect normal development and function of root, thereby affect the shoot growth and lead to semi-dwarf phenotype. Transfer BC88 fused with GFP protein into rice and tobacco epidermal cells show that, OsCesA9 is located at plasma membrane where cellulose synthesized, coinciding with that OsCesA9 is one of the essential functional CESA units for cellulose synthesis of secondary cell wall. These results provided new information for analysis of BC88 biological function and it’s of great significance for the further understanding of BC88 in regulating the secondary cell wall synthesis or other related process.

Key words: rice, BC88, OsCesA, expression, localization

摘要:

禾本科植物的脆秆突变体是进行次生细胞壁研究的理想材料。对水稻 bc88突变体的前期研究表明, BC88编码一个纤维素合酶催化亚基OsCesA9。通过水稻遗传转化 BC88启动子融合GUS的载体, 检测到BC88在根、茎、叶、鞘、花中均有表达,并且在茎和根中表达量较高,这可能是由于 BC88突变后影响了根系的正常发育及功能,进而影响地上部的生长,最终导致 bc88半矮表型。将 BC88GFP的融合表达载体转入水稻和烟草表皮细胞中,共聚焦显微观察显示,OsCesA9定位在质膜上。质膜是纤维素合成的主要场所,这充分说明了OsCesA9是水稻次生细胞壁中纤维素合成必不可少的纤维素合酶催化亚基之一。本研究为 BC88执行其生物学功能提供了新依据,对进一步认识该基因在调节次生细胞壁合成等方面的作用具有重要的意义。

关键词: 水稻, BC88, OsCesA, 表达, 定位

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