Mapping a QTL(qHTH5) for Heat Tolerance at the Heading Stage on Rice Chromosome 5 and Its Genetic Effect Analysis

doi:10.3969/j.issn.1001-7216.2015.02.002

Abstract

Abstract:

Using an interspecific near-isogenic line derived from Oryza sativa ssp. indica (Shuhui 527)×O. rufipogon Griff.(Yuanjiang common wild rice Hehuatang 3) as materials, a QTL for heat tolerance at the heading stage in the heat-tolerant near-isogenic rice line YJ10-03-01 was analyzed. A total of 360 simple sequence repeat(SSR) markers evenly distributed across 12 chromosomes were used to detect polymorphisms between YJ10-03-01 and indica restorer Shuhui 527, and nine were polymorphic. Single marker analysis revealed that markers on chromosome 5 were associated with heat tolerance at the heading stage using seed setting percentage as index for heat tolerance. By interval mapping using F₂ population(1,027 plants)from YJ10-03-01×Shuhui 527, a QTL for heat tolerance was detected on the short arm of chromosome 5. The QTL explained 8.6% and 19.4% of phenotypic variances in the F₂ and F₃ generations under heat stress (artificial climate chamber), respectively. Recombinant plants were screened for two flanking markers, RM7320 and RM7444, in F₃ generation, and substitution mapping suggested that the QTL was located in an interval of about 304.2 kb between markers RM592 and RM17921, we designated the QTL as qHTH5(quantitative trait locus for heat tolerance at the heading stage on chromosome 5).

Key words: rice(Oryza sativa L.), heat tolerance, QTL, gene mapping

摘要：

对元江普通野生稻(Oryza rufipogon Griff.) (简称元江普野)荷花塘3号为供体、籼稻(O. sativa ssp. indica)恢复系蜀恢527为轮回亲本构建的种间近等基因系群体进行数量性状位点(QTL)分析,在近等基因导入系YJ10-03-01中鉴定了一个抽穗扬花期耐热QTL。利用均匀分布在水稻12条染色体上的360个SSR标记检测近等基因系YJ10-03-01和籼稻恢复系蜀恢527,共获得9个多态性标记。单标记分析表明第5染色体短臂上的多态性标记与抽穗扬花期耐热性极显著相关。进一步在人工气候室模拟高温条件下处理YJ10-03-01与蜀恢527杂交得到F₂分离群体(1027个单株)并进行SSR标记分析,以水稻结实率为耐热指标,利用复合区间作图方法在第5染色体短臂上检测到一个抽穗扬花期耐热性QTL,暂命名为qHTH5。该QTL在F₂及F₃世代分别解释8.6% 和19.4%的表型变异。在F₃世代,继续利用目标区间标记RM7320和RM7444之间的SSR标记鉴定纯合重组体,利用置换作图法将QTL定位在约304.2 kb之内(RM592-RM17921)。

关键词: 水稻(Oryza sativa L.), 耐热性, QTL, 基因定位

CLC Number:

Zhi-bin CAO, Hong-wei XIE, Yuan-yuan NIE, Ling-hua MAO, Yong-hui LI, Yao-hui CAI. Mapping a QTL(qHTH5) for Heat Tolerance at the Heading Stage on Rice Chromosome 5 and Its Genetic Effect Analysis[J]. Chinese Journal OF Rice Science, 2015, 29(2): 119-125.

曹志斌, 谢红卫, 聂元元, 毛凌华, 李永辉, 蔡耀辉. 水稻抽穗扬花期耐热QTL(qHTH5) 定位及其遗传效应分析[J]. 中国水稻科学, 2015, 29(2): 119-125.

Figures/Tables 3

Table 1 Phenotypic performances of Shuhui 527 and YJ10-03-01 under high temperature and control temperature at heading stage.

性状 Trait	高温 High temperature			对照温 Control temperature
性状 Trait	蜀恢527 Shuhui 527	YJ10-03-01 (NIL)	RP-NIL	蜀恢 527 Shuhui 527	YJ10-03-01	RP-NIL
株高 Plant height/ cm	105.4±2.8	104.1±1.5	1.3	106.6±3.3	105.3±2.3	1.3
穗长 Panicle length/cm	25.5±0.7	24.9±0.4	0.6	25.1±1.2	25.6±1.4	-0.5
单株有效穗数 No. of effective panicles per plant	9.6±1.4	8.9±0.7	0.7	8.6±1.3	8.3±1.3	0.3
每穗实粒数 No. of filled grains per panicle	74.1±4.1	89.8±10.1	15.7^**	113.1±14.6	112.4±17.5	-0.7
每穗总粒数 No. of total grains per panicle	134.1±11.1	133.9±4.3	0.2	136.8±19.1	137.3±21.1	-0.5
结实率 Seed setting rate/%	55.2±1.2	67.1±1.3	-12.1^**	82.8±4.8	81.9±3.5	0.9
千粒重 1000-grain weight/g	26.5±0.6	28.8±0.5	-2.3^**	29.5±1.6	29.8±1.5	-0.3
单株产量 Grain yield per plant/g	19.5±4.0	23.8±3.5	-4.3^**	29.3±6.2	28.6±4.2	0.9

Fig. 1. Mapping of qHLH5 by a substitution mapping strategy. Linkage map of the QTLs region produced with 1027 F2 plants. The number of recombinants between adjacent markers is indicated in the linkage map. Progeny testing of F3 homozygous recombinants delimited the qHLH5 locus to the region between markers RM592 and RM17921. The 117 recombinants were grouped into 10 groups based on genotypes. The numbers of recombinants in each group and phenotypic difference of each group from the controls YJ10-03-01 and Shuhui 527 for mean seed setting rate are shown on the right. An ‘a’ following the phenotypic value indicates that the mean phenotypic value of recombinant is not significantly different from that of YJ10-03-01 at P< 0.05; a ‘b’indicates that the mean phenotypic value of recombinant is not significantly different from that of Shuhui 527 at P< 0.05.

Table 2 QTL analysis of seed setting rate under high temperature treatment in the F2 and F3 generations of YJ10-03-01/Shuhui 527 at heading stage.

群体 Population	区间 Interval	LOD^a	表型方差^b Phenotypic variance^b/%	加性效应^c Additive effect^c/%	显性效应^d Dominance effectd/%
F₂	RM592-RM7921	5.61	8.6	5.2	3.2
F₃	RM592-RM7921	8.90	19.4	11.3	2.9

References 29

[1]	Welch J R, Vincent J R, Auffhammer M, et al.Rice yields in tropical/subtropical Asia exhibit large but oppo-sing sensitivities to minimum and maximum temperatures.Proc Natl Acad Sci USA, 2010,107(33): 14562-14567.
[2]	Peng S, Huang J, Sheehy J E, et al.Rice yields decline with higher night temperature from global warming.Proc Natl Acad Sci USA, 2004, 101(27): 9971-9975.
[3]	Jagadish S V, Craufurd P Q, Wheeler T R.High temperature stress and spikelet fertility in rice(Oryza sativa L.).J Exp Bot, 2007, 58(07): 1627-1635.
[4]	曹云英,段骅,杨立年,等. 减数分裂期高温胁迫对耐热性不同水稻品种产量的影响及其生理原因.作物学报, 2008, 34(12): 234-242.
[5]	石舂林,金之庆,郑建初,等. 减数分裂期高温对水稻颖花结实率影响的定量分析. 作物学报, 2008, 34(04): 627-631.
[6]	邓运,田小海,吴晨,等. 热害胁迫条件下水稻花药发育异常的早期特征. 中国生态农业报, 2010, 18(02);377-383.
[7]	张彬, 丙雯交, 郑建初, 等. 水稻开花期花粉活力和结实率对高温的响应特征. 作物学报, 2007, 33(07): 1177-1181.
[8]	彭海燕,周曾査,赵永玲,等. 2003年夏季长江中下游地区异常高温的分析.气象学, 2005, 25(04): 4355-4361.
[9]	张涛,杨莉,蒋开锋,等. 水稻抽穗扬花期耐热性的QTL分析.分子植物种, 2008, 6(05): 867-873.
[10]	曹立勇,赵建根,占小登,等. 水稻耐热性的QTL定位及耐热性与光合速率的相关性. 中国水稻科学, 2003, 17(03): 223-227.
[11]	陈庆全,余四斌, 李春海, 等. 水稻抽穗开花期耐热性QTL的定位分析. 中国农业科学, 2008, 41(2): 315-321.
[12]	Xiao Y, Pan Y, Luo L, et al.Quantitative trait loci associated with seed set under high temperature stress at the flowering stage in rice(Oryza sativa L.).Euphytica, 2011, 178(3): 331-338.
[13]	盘毅,罗丽华,邓化冰,等. 水稻开花期高温胁迫下的花粉育性QTL定位.中国水稻科学, 2011, 25(1):99-102.
[14]	王玉平,李仕贵,黎汉云,等. 高配合力优质水稻恢复系蜀恢527的选育与利用. 杂交水稻, 2004, 19(4): 12-14.
[15]	Rogers S O, Bendich A J.Extraction of total cellular DNA from plants, algae and fungi//Plant Molecular Biology Manual. Springer Netherlands, 1994: 183-190.
[16]	Lander E S, Green P, Abrahamson J, et al.MAPMAKER: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations.Genomics, 1987, 1: 174-181.
[17]	Basten C J, Weir B S, Zeng Z B.QTL cartographer, Version 1.15. Raleigh, NC,USA:Department of Statistics, Northcarelina State University,2001.
[18]	van Ooijen J W. MapQTL5.0, software for the mapping of quantitative trait loci in experimental populations. Kyazma BV, Wageningen, 2004: 63.
[19]	Wissuwa M, Wegner J, Ae N, et al.Substitution mapping of Pup1: A major QTL increasing phosphorus uptake of rice from a phosphorus-deficient soil.Theor Appl Genet, 2002, 105(6/7): 890-897.
[20]	舒孝顺,陈良碧. 高温敏感不育水稻育性敏感期幼穗和叶片中的总 RNA 含量变化. 植物生理学通讯, 1999, 35(2): 108-110.
[21]	郑建初,张彬,陈留根,等. 抽穗期高温对水稻产量构成要素和稻米品质的影响及其基因型差异. 江苏农业学报, 2005, 21(4): 249-254.
[22]	Andronova N G, Schlesinger M E.Causes of global temperature changes during the 19th and 20th centuries.Geophysical Res Lett, 2000, 27(14): 2137-2140.
[23]	Crowley T J.Causes of climate change over the past 1000 years.Science, 2000, 289(5477): 270-277.
[24]	王亚伟,翟盘茂,田华. 近40年南方高温变化特征与2003年的高温事件. 气象, 2006, 32(10): 27-33.
[25]	Matsui T, Omasa K, Horie T.The difference in sterility due to high temperatures during the flowering period among japonica rice varieties.Plant Prod Sci, 2001, 4(2): 90-93.
[26]	森谷国男,徐正进. 水稻高温胁迫抗性遗传育种研究概况. 杂交水稻, 1992, 33(1): 47-48.
[27]	赵志刚,江玲,肖应辉,等. 水稻孕穗期耐热性QTLs分析. 作物学报, 2006, 32(5): 640-644.
[28]	奎丽梅,谭禄宾,涂建,等. 云南元江野生稻抽穗开花期耐热QTL定位. 农业生物技术学报, 2008, 16(3): 461-464.
[29]	Temnykh S, Park W D, Ayres N, et al.Mapping and genome organization of microsatellite sequences in rice (Oryza sativa L.).Theor Appl Genet, 2000, 100(5): 697-712.