Chinese Journal OF Rice Science ›› 2015, Vol. 29 ›› Issue (2): 111-118.DOI: 10.3969/j.issn.1001-7216.2015.02.001
• Orginal Article • Next Articles
Shu-jun WU1, Jie YANG2, Ying YAN1, Li-xia ZHANG1, Fang-jun FAN2, Jin-yan ZHU2, Wen-qi LI2, Wei-gong ZHONG2, Li-ming CAO1,*(), Jun WANG2,*()
Received:
2014-09-23
Revised:
2014-12-10
Online:
2015-03-10
Published:
2015-03-10
Contact:
Li-ming CAO, Jun WANG
吴书俊1, 杨杰2, 闫影1, 张丽霞1, 范方军2, 朱金燕2, 李文奇2, 仲维功2, 曹黎明1,*(), 王军2,*()
通讯作者:
曹黎明,王军
基金资助:
CLC Number:
Shu-jun WU, Jie YANG, Ying YAN, Li-xia ZHANG, Fang-jun FAN, Jin-yan ZHU, Wen-qi LI, Wei-gong ZHONG, Li-ming CAO, Jun WANG. Physiological Characterization and Gene Identification of a Yellow-Green Leaf Mutant ygl11(t) in Rice[J]. Chinese Journal OF Rice Science, 2015, 29(2): 111-118.
吴书俊, 杨杰, 闫影, 张丽霞, 范方军, 朱金燕, 李文奇, 仲维功, 曹黎明, 王军. 水稻黄绿叶突变体ygl11(t)的生理特性和基因克隆[J]. 中国水稻科学, 2015, 29(2): 111-118.
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URL: http://www.ricesci.cn/EN/10.3969/j.issn.1001-7216.2015.02.001
Fig. 1. Phenotype of the yellow-green leaf mutant and the wild type at different stages. A, Plant at tillering stage; B, Plant at grain filling stage; C, Leaf morphology at mature stages.
材料 Material | 株高 Plant height /cm | 有效穗数 Effective panicle number | 穗长 Panicle length /cm | 总粒数 Total grain number | 结实率 Seed setting rate/% | 千粒重 1000-grain weight/g |
---|---|---|---|---|---|---|
南粳41 Nanjing 41 | 93.3±0.5 | 10.3±0.3 | 17.4±1.2 | 162.0±9.5 | 89.5±2.1 | 27.7±0.3 |
ygl11(t) | 108.1±0.7** | 9.2±0.2** | 21.5±0.9** | 105.1±6.3** | 87.0±2.3 | 26.8±0.3 |
Table 1 Comparison of major agronomic traits between the ygl11(t)mutant and the wild type.
材料 Material | 株高 Plant height /cm | 有效穗数 Effective panicle number | 穗长 Panicle length /cm | 总粒数 Total grain number | 结实率 Seed setting rate/% | 千粒重 1000-grain weight/g |
---|---|---|---|---|---|---|
南粳41 Nanjing 41 | 93.3±0.5 | 10.3±0.3 | 17.4±1.2 | 162.0±9.5 | 89.5±2.1 | 27.7±0.3 |
ygl11(t) | 108.1±0.7** | 9.2±0.2** | 21.5±0.9** | 105.1±6.3** | 87.0±2.3 | 26.8±0.3 |
时期 Stages | 材料 Material | 色素含量 Pigment content / (mg·g-1) | 叶绿素a/叶绿素b Chlorophyll a/ Chlorophyll b | |||
---|---|---|---|---|---|---|
总叶绿素 Total chlorophyll | 叶绿素a Chlorophyll a | 叶绿素b Chlorophyll b | 类胡萝卜素 Carotenoid | |||
苗期 Seedling | NJ41 | 3.83±0.12 | 3.04±0.11 | 0.79±0.05 | 0.67±0.02 | 3.87±0.26 |
ygl11(t) | 2.86±0.11** | 2.66±0.10** | 0.20±0.01** | 0.65±0.02 | 13.02±0.45** | |
分蘖盛期 Full tillering | NJ41 | 3.94±0.16 | 3.06±0.13 | 0.88±0.03 | 0.67±0.03 | 3.49±0.05 |
ygl11(t) | 1.80±0.02** | 1.69±0.02** | 0.11±0.00** | 0.42±0.01** | 14.99±0.52** | |
齐穗期 Full heading | NJ41 | 3.84±0.03 | 3.01±0.02 | 0.83±0.01 | 0.69±0.01 | 3.60±0.04 |
ygl11(t) | 1.88±0.05** | 1.76±0.05** | 0.12±0.01** | 0.43±0.01** | 14.62±1.00** |
Table 2 Chlorophyll and carotenoid contents of ygl11(t) and the wild type at different growth stages.
时期 Stages | 材料 Material | 色素含量 Pigment content / (mg·g-1) | 叶绿素a/叶绿素b Chlorophyll a/ Chlorophyll b | |||
---|---|---|---|---|---|---|
总叶绿素 Total chlorophyll | 叶绿素a Chlorophyll a | 叶绿素b Chlorophyll b | 类胡萝卜素 Carotenoid | |||
苗期 Seedling | NJ41 | 3.83±0.12 | 3.04±0.11 | 0.79±0.05 | 0.67±0.02 | 3.87±0.26 |
ygl11(t) | 2.86±0.11** | 2.66±0.10** | 0.20±0.01** | 0.65±0.02 | 13.02±0.45** | |
分蘖盛期 Full tillering | NJ41 | 3.94±0.16 | 3.06±0.13 | 0.88±0.03 | 0.67±0.03 | 3.49±0.05 |
ygl11(t) | 1.80±0.02** | 1.69±0.02** | 0.11±0.00** | 0.42±0.01** | 14.99±0.52** | |
齐穗期 Full heading | NJ41 | 3.84±0.03 | 3.01±0.02 | 0.83±0.01 | 0.69±0.01 | 3.60±0.04 |
ygl11(t) | 1.88±0.05** | 1.76±0.05** | 0.12±0.01** | 0.43±0.01** | 14.62±1.00** |
Fig. 3. Ultrastructure of chloroplasts in the mesophyll cells of ygl11(t)and the wild type. A and B, Chloroplast structure of the wild type Nanjing 41; C and D, Chloroplast structure of the ygl11(t) mutant.
组合 Combination | 总株数 Total number of plants | 正常植株 No. of normal plants | 黄绿植株 No. of yellow green plants | χ2(3:1) | |
---|---|---|---|---|---|
ygl11(t)/9311 | 1713 | 1305 | 408 | 1.21 | 3.84 |
9311/ygl11(t) | 1243 | 942 | 301 | 0.37 |
Table 3 Segregation of yellow-green leaf in F2 populations.
组合 Combination | 总株数 Total number of plants | 正常植株 No. of normal plants | 黄绿植株 No. of yellow green plants | χ2(3:1) | |
---|---|---|---|---|---|
ygl11(t)/9311 | 1713 | 1305 | 408 | 1.21 | 3.84 |
9311/ygl11(t) | 1243 | 942 | 301 | 0.37 |
引物名称 Primer name | 类型 Type | 正向引物(5'-3') Forward primer (5' - 3') | 反向引物(5'-3') Reverse primer (5'-3') | 酶切位点 Enzyme site |
---|---|---|---|---|
I1 | InDel | CTCCGTCACTCTACTACCTGC | CTGCTGCGACATCTCACG | - |
I4 | InDel | ATATTGCAGGCCAACCATTC | CTCATTCTCATCCCTGTGCC | - |
I6 | InDel | GACGGACTTGAAGCGTAGCA | GCTGCATGTAGGACGACACC | - |
I11 | InDel | TTTAGGTGGAGCTGAAACTGT | TAAGCCAAACGATGGGAAC | - |
D3 | CAPs | CTCCCGAATCGAGTGGAATA | CAGATCGAGGGTGAAGAACC | BSHⅠ |
D5 | CAPs | ACAGCGTAACATTCAGCACC | CAACTTCTTGAGGAAAGGGTTA | SspⅠ |
CAO1-1 | CAO1测序 | CGGCTGAGATGCGTTCC | GCACCGCATACACCTTGATT | - |
CAO1-2 | CAO1测序 | CCCAGTAACCACAGGCTTATCT | ACTGAGCCAAGATGAAGAGGG | - |
CAO1-3 | CAO1测序 | AAAGGATGGGAGACCTGGAT | TTCATCTCGCCAAGAAATACG | - |
CAO1-4 | CAO1测序 | GGCAGCAAGAACGGATGAT | AGCACATAGATGGATAGAGGGAT | - |
CAO2-1 | CAO2测序 | TATGGAACGCAGGAACGAG | TATGGAACGCAGGAACGAG | - |
CAO2-2 | CAO2测序 | AGGTGGTAGAGGTTCTCAAATC | CATCAGTTGAATACTCCCACC | - |
CAO2-3 | CAO2测序 | AAAGGCTCGGAACTGACATA | CGACAAGAGTTGAAAGAGGC | - |
CAO2-4 | CAO2测序 | TGCCATTCAATAACCAAAGTG | CAAAGCCGACGCAGGTAG | - |
Table 4 The markers used for YGL11(t) gene mapping and sequencing.
引物名称 Primer name | 类型 Type | 正向引物(5'-3') Forward primer (5' - 3') | 反向引物(5'-3') Reverse primer (5'-3') | 酶切位点 Enzyme site |
---|---|---|---|---|
I1 | InDel | CTCCGTCACTCTACTACCTGC | CTGCTGCGACATCTCACG | - |
I4 | InDel | ATATTGCAGGCCAACCATTC | CTCATTCTCATCCCTGTGCC | - |
I6 | InDel | GACGGACTTGAAGCGTAGCA | GCTGCATGTAGGACGACACC | - |
I11 | InDel | TTTAGGTGGAGCTGAAACTGT | TAAGCCAAACGATGGGAAC | - |
D3 | CAPs | CTCCCGAATCGAGTGGAATA | CAGATCGAGGGTGAAGAACC | BSHⅠ |
D5 | CAPs | ACAGCGTAACATTCAGCACC | CAACTTCTTGAGGAAAGGGTTA | SspⅠ |
CAO1-1 | CAO1测序 | CGGCTGAGATGCGTTCC | GCACCGCATACACCTTGATT | - |
CAO1-2 | CAO1测序 | CCCAGTAACCACAGGCTTATCT | ACTGAGCCAAGATGAAGAGGG | - |
CAO1-3 | CAO1测序 | AAAGGATGGGAGACCTGGAT | TTCATCTCGCCAAGAAATACG | - |
CAO1-4 | CAO1测序 | GGCAGCAAGAACGGATGAT | AGCACATAGATGGATAGAGGGAT | - |
CAO2-1 | CAO2测序 | TATGGAACGCAGGAACGAG | TATGGAACGCAGGAACGAG | - |
CAO2-2 | CAO2测序 | AGGTGGTAGAGGTTCTCAAATC | CATCAGTTGAATACTCCCACC | - |
CAO2-3 | CAO2测序 | AAAGGCTCGGAACTGACATA | CGACAAGAGTTGAAAGAGGC | - |
CAO2-4 | CAO2测序 | TGCCATTCAATAACCAAAGTG | CAAAGCCGACGCAGGTAG | - |
Fig. 4. Fine mapping and cloning of the YGL11(t) gene. A,Rough mapping of YGL11(t) on chromosome 10; B, Fine mapping of YGL11(t) gene; C,The ORFs in the 58.1 kb region; D, 2 bp deletion between ygl11(t)and Nanjing 41 and Nipponbare.
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