Chinese Journal OF Rice Science ›› 2015, Vol. 29 ›› Issue (2): 111-118.DOI: 10.3969/j.issn.1001-7216.2015.02.001

• Orginal Article •     Next Articles

Physiological Characterization and Gene Identification of a Yellow-Green Leaf Mutant ygl11(t) in Rice

Shu-jun WU1, Jie YANG2, Ying YAN1, Li-xia ZHANG1, Fang-jun FAN2, Jin-yan ZHU2, Wen-qi LI2, Wei-gong ZHONG2, Li-ming CAO1,*(), Jun WANG2,*()   

  1. 1 Crop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China
    2Institute of Food Crops, Jiangsu Academy of Agricultural Sciences/Nanjing Branch of Chinese National Center for Rice Improvement, Nanjing 210014, China
  • Received:2014-09-23 Revised:2014-12-10 Online:2015-03-10 Published:2015-03-10
  • Contact: Li-ming CAO, Jun WANG

水稻黄绿叶突变体ygl11(t)的生理特性和基因克隆

吴书俊1, 杨杰2, 闫影1, 张丽霞1, 范方军2, 朱金燕2, 李文奇2, 仲维功2, 曹黎明1,*(), 王军2,*()   

  1. 1上海市农业科学院 作物育种栽培研究所, 上海 201403
    2江苏省农业科学院 粮食作物研究所/国家水稻改良中心南京分中心, 南京210014
  • 通讯作者: 曹黎明,王军
  • 基金资助:
    上海市水稻产业技术体系建设项目[沪农科产字(2014)第3号];上海市科委重点科技攻关项目(10391900602);国家自然科学基金资助项目(31101131);国家科技支撑计划重大项目(2011BAD16B03);江苏省农业自主创新资金资助项目[CX(12)1003]

Abstract:

A spontaneous mutant showing yellow-green leaf throughout the growth period, designated as ygl11(t), was screened and identified from rice cultivar Nanjing 41. This mutant was grown for several generations and showed stable mutation phenotype. The ygl11(t) mutant has reduced chlorophyll accumulation and abnormal chloroplast development. The average contents of total chlorophyll, chlorophyll a, chlorophyll b, and carotene in the ygl11(t) mutant were 45.7% to 74.7%, 55.2% to 87.5%, 12.5% to 25.3%, and 62.3% to 97.0% of these in wild type, respectively, at the stages of seedling, tillering and heading. The leaf net photosynthetic rate of the ygl11(t) was significantly higher at maximum tillering stage and slightly lower at 10 days after flowering than that of wild type. Ultrastructure analysis revealed that the grana lamellae of chloroplast thylakoid were less in number and abnormally developed in the ygl11(t) mutant. Genetic analysis suggested that the phenotype of ygl11(t) was controlled by a recessive nuclear gene. YGL11(t)was prelimarily mapped on the long arm of rice chromosome 10, and was finally narrowed to a 58.1 kb region containing six open reading frames (ORFs). All the six ORFs were amplified and sequenced. A 2-bp deletion was found in the 9th exon of the OsCAO1gene, encoding a chlorophyll a oxygenase 1, which results in a premature stop codon and protein truncation in the ygl11(t) mutant. These results suggest that the OsCAO1 gene is the candidate of YGL11(t).

Key words: rice, yellow-green leaf mutant, fine mapping, gene, OsCAO1

摘要:

在水稻品种南粳41中发现了一个黄绿叶自然突变体,经过多代连续自交形成了稳定的突变系,命名为ygl11(t),ygl11(t)整个生育期叶片都表现为黄绿色。对苗期、分蘖盛期、齐穗期突变体和野生型的叶绿素含量进行测定,ygl11(t) 的叶绿素含量是野生型的45.7%~74.7%,叶绿素a 含量是野生型的55.2%~87.5%,叶绿素b含量是野生型的12.5%~25.3%,ygl11(t) 的类胡萝卜素的含量是野生型的62.3%~97.0%。ygl11(t)在分蘖盛期的净光合速率显著高于野生型,花后10 d,ygl11(t)的净光合速率比野生型略低。对突变体叶片中叶绿体的超微结构进行观察,发现突变体叶绿体内的类囊体基粒片层数目减少且严重扭曲变形。遗传分析表明,ygl11(t)叶色性状受1对隐性核基因控制。利用SSR分子标记将YGL11(t)初步定位在水稻第10染色体的长臂上,进一步利用新开发的InDel和CAPS标记将YGL11(t)定位在58.1 kb的物理距离内。对该区段内存在的开放阅读框进行序列分析,发现突变体ygl11(t) 中编码叶绿素a氧化酶(chlorophyll a oxygenase 1)基因(OsCAO 1) 的第9个外显子存在2个碱基缺失,从而导致提前出现终止密码子,初步分析OsCAO1即为YGL11(t)的候选基因。

关键词: 水稻, 黄绿叶突变体, 精细定位, 基因, OsCAO1

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