Genetic Analysis and Gene Mapping of a New SemiDwarf and Small Panicle Mutant in Rice

doi:10.3969/j.issn.10017216.2011.06.017

Chinese Journal of Rice Science ›› 2011, Vol. 25 ›› Issue (6): 677-680.DOI: 10.3969/j.issn.10017216.2011.06.017

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Genetic Analysis and Gene Mapping of a New SemiDwarf and Small Panicle Mutant in Rice

WANG Qing ^1,2,3, WANG Dekai ^2,* , TAO Yuezhi ^1,2,*

¹ College of Chemistry and Life Sciences, Zhejiang Normal University， Jinhua 321004， China; ² Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; ³Wuxi Qiushi BioAgricultural Co., Ltd.;

Received:2010-12-22 Revised:2011-02-25 Online:2011-11-10 Published:2011-11-10
Contact: WANG Dekai2,* , TAO Yuezhi1,2,*

一个新的水稻半矮化小穗突变体的遗传分析与基因定位

汪庆^1,2,3汪得凯^2,*陶跃之^1,2,*

¹浙江师范大学化学与生命科学学院，浙江金华 321004； ² 浙江省农业科学院作物与核技术利用研究所, 浙江杭州 310021； ³无锡求是生物农业有限公司；

通讯作者: 汪得凯2,*陶跃之1,2,*
基金资助:
浙江省自然科学基金重点项目（Z3110509）；国家转基因生物新品种科技重大专项（2008ZX08010002）

Abstract

Abstract: A semidwarf and small panicle mutant was obtained from a japonica rice variety Zhonghua 11 through TDNA transformation. The mutant, characterized by semidwarf plant, abnormal small and semienclosed panicle, and poor growth potential was designated as sdsp2(semidwarf and small panicle 2). Genetic analysis indicated that the mutant was controlled by a single recessive gene. A genetic linkage map was constructed using an F2 mapping population derived from a cross between the sdsp2 mutant and an indica variety Longtefu B on chromosome 6. The gene was finally located on a 116kb region between the STS markers RH632 and RH640，where 14 ORFs were predicted based on Rice Genome Annotation．

Key words: rice, semidwarf, small panicle, mutant, gene mapping

摘要： 从粳稻中花 11转基因后代中发现了一个半矮化小穗突变体，表现为植株半矮化、生长势弱、半包茎穗、穗型变小等特点，将其命名为sdsp2(semidwarf and small panicle 2)。遗传分析显示，该突变体表型受1对隐性核基因控制。以sdsp2突变体为母本与龙特甫B杂交构建F2分离群体，将该基因定位在水稻第6染色体的RH632和RH640之间的116 kb的物理距离内。通过水稻基因组注释系统在此区域预测到14个开放阅读框，未发现与已报道穗型发育相关基因的同源基因。

关键词: 水稻, 半矮化, 小穗, 突变体, 基因定位

CLC Number:

WANG Qing1,2,3, WANG Dekai2,* , TAO Yuezhi1,2,* . Genetic Analysis and Gene Mapping of a New SemiDwarf and Small Panicle Mutant in Rice[J]. Chinese Journal of Rice Science, 2011, 25(6): 677-680.

汪庆1,2,3汪得凯2,*陶跃之1,2,*. 一个新的水稻半矮化小穗突变体的遗传分析与基因定位[J]. 中国水稻科学, 2011, 25(6): 677-680.

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Genetic Analysis and Gene Mapping of a New SemiDwarf and Small Panicle Mutant in Rice

一个新的水稻半矮化小穗突变体的遗传分析与基因定位

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