Application  of Capillary Electrophoresis Detection   with Fluorescent SSR Markers in Rice DNA Fingerprint Identification

doi:10.3969/j.issn.10017216.2011.06.016

Chinese Journal of Rice Science ›› 2011, Vol. 25 ›› Issue (6): 672-676.DOI: 10.3969/j.issn.10017216.2011.06.016

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Application of Capillary Electrophoresis Detection with Fluorescent SSR Markers in Rice DNA Fingerprint Identification

CHENG Benyi, XIA Junhui, GONG Junyi, YANG Shihua^*

China National Rice Research Institute, Hangzhou 310006, China

Received:2011-04-02 Revised:2011-08-06 Online:2011-11-10 Published:2011-11-10
Contact: YANG Shihua*

SSR荧光标记毛细管电泳检测法在水稻DNA指纹鉴定中的应用

程本义,夏俊辉,龚俊义,杨仕华^*

中国水稻研究所，浙江杭州 310006；

通讯作者: 杨仕华*
基金资助:
国家科技支撑计划资助项目(2011BAD35B09)；中央级公益性科研院所专项资金资助项目(2009RG0053)；农业部农产品质量安全监管(种子管理)项目

Abstract

Abstract: With 16 hybrid rice male sterile lines, restorer lines and combinations as materials, a capillary electrophoresis detection method with fluorescent SSR markers in rice was primarily developed. The accurate size of DNA fragment could be detected by the capillary electrophoresis detection method with fluorescent SSR markers. The capillary electrophoresis detection has the advantages of data accuracy and efficiency compared with polyacrylamide gel electrophoresis detection. It provides a feasible and reliable method for rice DNA fingerprint identification.

Key words: rice, fluorescent SSR marker, capillary electrophoresis, DNA fingerprint identification

摘要： 以16个杂交水稻不育系、恢复系及组合为材料，初步建立了水稻品种SSR荧光标记毛细管电泳检测方法。与常规凝胶电泳检测方法相比，荧光标记毛细管电泳检测方法可以读出目标DNA片段的准确大小，检测数据更为精确，检测效率更高。常规凝胶电泳检测方法验证表明，利用SSR荧光标记毛细管电泳检测方法进行水稻品种DNA指纹鉴定，方法可行、结果可靠。

关键词: 水稻, SSR荧光标记, 毛细管电泳, DNA指纹鉴定

CLC Number:

CHENG Benyi, XIA Junhui, GONG Junyi, YANG Shihua* . Application of Capillary Electrophoresis Detection with Fluorescent SSR Markers in Rice DNA Fingerprint Identification[J]. Chinese Journal of Rice Science, 2011, 25(6): 672-676.

程本义,夏俊辉,龚俊义,杨仕华* . SSR荧光标记毛细管电泳检测法在水稻DNA指纹鉴定中的应用[J]. 中国水稻科学, 2011, 25(6): 672-676.

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Application of Capillary Electrophoresis Detection with Fluorescent SSR Markers in Rice DNA Fingerprint Identification

SSR荧光标记毛细管电泳检测法在水稻DNA指纹鉴定中的应用

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