Effect of High Temperature Stress on Physiological Characteristics of Spikelet of Rice During Flowering Stage

doi:10.16819/j.1001-7216.2016.5178

Abstract

Abstract:

To ascertain the effect of high temperature stress on the physiological characteristics of rice spikelet at flowing stage, wild type Zhonghua 11(ZH11) and its heat-resistant mutant (hst) were exposed to heat stress (40℃,6 h per day) for 7 days in growth chambers. The results showed that under heat stress the chlorophyll content in flag leaf, soluble protein and soluble sugar contents in spikelet of rice decreased obviously with lower decline in the mutant hst compared to the wild type ZH11. While malonyldialdehyde (MDA), hydrogen peroxide(H₂O₂) and free proline contents in spikelets increased significantly with greater increase in wild type ZH11 than in the mutant hst. Superoxide dismutase (SOD), catalase (CAT) and peroxidase(POD) activities showed different responses to high temperature stress, and the correlation between the enzyme activities and the thermal sensitivity would be further investigated. Under high temperature stress, higher photosynthesis efficiency, better osmotic adjustment ability as well as lower MDA and H₂O₂ content of spikelets in the mutant could be the physiological basis of high temperature tolerance.

Key words: rice, flowering stage, high temperature stress, spikelet, physiological characteristics

摘要：

以野生型水稻中花11及其耐热型突变体(hst)为材料,于抽穗扬花期高温处理7 d (40℃,每天处理6 h),探讨开花期水稻颖花生理特性与耐热性之间的关系。研究表明,花期高温导致剑叶叶绿素含量、颖花可溶性蛋白和可溶性糖含量均显著下降,突变体hst的降幅明显小于野生型中花11;同时,高温下颖花中丙二醛(MDA)、过氧化氢(H₂O₂)及游离脯氨酸含量均显著增加,而突变体hst的增幅明显低于野生型中花11。在抗氧化酶活性方面,超氧化物歧化酶(SOD)、过氧化物酶(POD)及过氧化氢酶(CAT)活性对水稻开花期高温胁迫的响应有所不同,其变化与耐热性之间的相关性有待进一步验证。高温胁迫下水稻剑叶中保持较稳定的光合作用,颖花中保持较多的渗透调节物质及较低的MDA和H₂O₂含量,是突变体耐高温的生理基础。

关键词: 水稻, 抽穗扬花期, 高温胁迫, 颖花, 生理特性

CLC Number:

Xu LAN, Zheng-dong GU, Yan-fei DING, Ke WANG, Qiong JIANG, Cheng* ZHU. Effect of High Temperature Stress on Physiological Characteristics of Spikelet of Rice During Flowering Stage[J]. Chinese Journal OF Rice Science, 2016, 30(6): 637-646.

兰旭, 顾正栋, 丁艳菲, 王珂, 江琼, 朱诚. 花期高温胁迫对水稻颖花生理特性的影响[J]. 中国水稻科学, 2016, 30(6): 637-646.

Figures/Tables 11

Fig. 1. Effect of high temperature stress on the fertilized rate of spikelets at the flowering stage. ZH11-T, Wild type under high temperature stress;hst-T, Mutant under high temperature stress; ZH11-CK, Wild type under natural condition; hst-CK, Mutant under natural condition; All data are mean±standard deviation; n=30; The same letter indicates no significant difference at 0.05 significant level. The same below.

Table 1 Effect of high temperature stress on rice seed-setting rate,1000-grain weight and yield per plant.

品系 Line	处理 Treatment	千粒重 1000-grain weight/g	每穗实粒数 Grain number per panicle	单株产量 Yield per plant/g	结实率 Seed-setting rate/%	热敏感指数 HSI/%
中花11 Zhonghua 11	适温CK	23.92±0.02 c	48.6±5.6 a	20.94±1.34 b	68.1±9.5 a	80.24
	高温T	26.41±0.07 a	10.0±2.5 c	4.80±0.48 d	13.4±2.9 c
突变体 hst	适温CK	24.09±0.01 b	57.9±6.0 a	29.26±1.71 a	72.8±10.3 a	46.87
	高温T	24.19±0.09 b	27.1±3.7 b	13.79±0.99 c	38.4±4.8 b

Fig. 2. Effect of high temperature stress on chlorophyll content in flag leaf of rice at the flowering stage.

Fig. 3. Effect of high temperature stress on soluble protein content in spikelets of rice at the flowering stage.

Fig.4. Effect of high temperature stress on soluble sugar content in spikelets of rice at the flowering stage.

Fig. 5. Effect of high temperature stress on free proline content in spikelets of rice at the flowering stage.

Fig. 6. Effect of high temperature stress on SOD activity in spikelets of rice at the flowering stage.

Fig. 7. Effect of high temperature stress on POD activity in spikelets of rice at the flowering stage.

Fig. 8. Effect of high temperature stress on CAT activity in spikelets of rice at the flowering stage.

Fig. 9. Effect of high temperature stress on H2O2 content in spikelets of rice at the flowering stage.

Fig. 10. Effect of high temperature stress on MDA content in spikelets of rice at the flowering stage.

References 39

[1]	Pachauri R K. Climate change 2007. Synthesis report, Contribution of Working Groups I, II and III to the fourth assessment report. 2008.
[2]	Wang S W, Ye J L.Analysis of global warming during the last one hundred years.Sci Atmos Sin, 1995, 19(5): 545-553.
[3]	Pens S B,Huang J L,Sheehy J E,et al.Rice yields decline with high temperature from global warming.PNAS, 2004, 101(27): 9971-9975.
[4]	吉林省教育系统科教兴农专家组. 水稻栽培技术. 长春: 吉林出版集团有限责任公司, 吉林科学技术出版社, 2008: 56-57.
	The education system in Jilin Province by science and technology expert group. Rice Cultivation Technology. Changchun: Jilin publishing Refco Group Ltd, Jilin Science and Technology Press, 2008: 56-57. (in Chinese)
[5]	曲爱丽. 水稻花期耐高温突变体的遗传分析. 杭州: 中国计量学院, 2014.
	Qu A L.Genetic analysis of the rice(Oryz sativa L. ) high tolerance mutant during anthesis. Hangzhou: China Jiliang University, 2014. (in Chinese with English abstract)
[6]	符冠富, 张彩霞, 杨雪芹, 等. 水杨酸减轻高温抑制水稻颖花分化的作用机理研究. 中国水稻科学, 2015, 29(6): 637-647.
	Fu G F, Zhang C X, Yang X Q, et al.Action mechanism by which SA alleviates high temperature-induced inhibition to spikelet differentiation.Chin J Rice Sci, 2015, 29(6): 637-647. (in Chinese with English abstract)
[7]	Satake T, Yoshida S.High temperature-induced sterility in indica rice at flowering.Jpn J Crop Sci, 1978, 47: 6-17.
[8]	周建霞, 张玉屏, 朱德峰, 等. 高温后移对水稻颖花败育的影响. 中国农业气象, 2014, 35(5): 544-548.
	Zhou J X, Zhang Y P, Zhu D F, et al.Effects of high temperature on rice spikelet sterility at flowering stage.Chin J Agrometeorol, 2014, 35(50): 544-548. (in Chinese with English abstract)
[9]	谢晓金, 申双和, 李秉柏, 等. 抽穗期高温胁迫对水稻开花结实的影响. 中国农业气象, 2009, 30(2): 252-256.
	Xie X J, Shen S H, Li B B, et al.Influences of high temperature stress on blooming and seed setting of rice during heading stage.Chin J Agrometeorol, 2009, 30(2): 252-256. (in Chinese with English abstract)
[10]	张桂莲, 张顺堂, 肖浪涛, 等. 花期高温胁迫对水稻花药生理特性及花粉性状的影响. 作物学报, 2013, 39(1): 177-183.
	Zhang G L, Zhang S T, Xiao L T, et al.Effect of high temperature stress on physiological characteristics of anther and pollen traits of rice at flowering stages.Acta Agron Sin, 2013, 39(1): 177-183. (in Chinese with English abstract)
[11]	Jagadish S V K, Craufurd P Q, Wheeler T R.High temperature stress and spikelet fertility in rice.J Exp Bot, 2007, 58: 1627-1635.
[12]	Prasad P V V,Boote K J,Allen L H Jr,et al.Species,ecotype and cultivar differences in spikelet fertility and harvest index of rice in response to high temperature stress.Field Crops Res, 2006, 95: 398-411.
[13]	周建霞, 张玉屏, 朱德峰, 等. 高温下水稻开花习性对受精率的影响. 中国水稻科学, 2014, 28(3): 297-303.
	Zhou J X, Zhang Y P, Zhu D F, Influence of flowing characteristics on spikelet fertility under high temperature.Chin J Rice Sci, 2014, 28(3): 297-303. (in Chinese with English abstract)
[14]	Rang Z W, Jagadish S, Zhou Q M, et al.Effect of high temperature and water stress on pollen germination and spikelet fertility in rice.Environ Exp Bot, 2011, 70(1): 58-65.
[15]	蔡冲, 姜维梅, 孙骏威. 植物生物学实验. 北京: 北京师范大学出版社, 2013: 55-58.
	Cai C, Jiang W M, Sun J W. Plant Biological Experiment.Beijing: Beijing Normal University Press, 2013: 55-58. (in Chinese)
[16]	胡雁春. 模拟酸雨对三叶草种子萌发及幼苗生理指标的影响. 安徽农业科学, 2014, 42(1): 85-86, 89.
	Hu Y C.Study on the influence of simulated acid rain on clover.J Anhui Agric Sci, 2014, 42(1): 85-86, 89. (in Chinese with English abstract)
[17]	刘海英, 王华华, 崔长海, 等. 可溶性糖含量测定(蒽酮法)实验的改进. 实验室科学, 2013, 16(2): 19-20.
	Liu H Y, Wang H H, Cui C H, et al.Experiment improvement of the soluble sugar content determination by enthrone colorimetric method.Lab Sci, 2013, 16(2): 19-20. (in Chinese with English abstract)
[18]	赵英永, 戴云, 崔秀明, 等. 考马斯亮蓝G-250染色法测定草乌中可溶性蛋白质含量. 云南民族大学学报:自然科学版, 2006, 15(3): 235-237.
	Zhao Y Y, Dai Y, Cui X M, et al.Determination of protein contents of radix aconiti kusnezoffii using Coomassie Brillant Blue G-250 dye binding.J Yunnan Nat Univ: Nat Sci Ed), 2006, 15(3): 235-237. (in Chinese with English abstract)
[19]	张桂莲, 陈立云, 雷东阳, 等. 水稻耐热性研究进展. 杂交水稻, 2005, 20(1): 1-5.
	Zhang G L, Chen L Y, Lei D Y, et al.Progress in research on heat tolerance in Rice.Hybrid Rice, 2005, 20(1): 1-5(in Chinese with English abstract).
[20]	李轶冰, 杨顺强, 任广鑫, 等. 低温处理下不同禾本科牧草的生理变化及其抗寒性比较. 生态学报, 2009, 29(3): 1341-1347.
	Li Y B, Yang S Q, Ren G X, et al.Changes analysis in physiological properties of several gramineous grass species and cold-resistance comparison on under cold stress.Acta Ecol Sin, 2009, 29(3): 1341-1347. (in Chinese with English abstract)
[21]	和红云, 薛琳, 田丽萍, 等. 低温胁迫对甜瓜幼苗根系活力及渗透调节物质的影响. 石河子大学学报: 自然科学版, 2008, 26(5): 583-586.
	He H Y, Xue L, Tian L P, et al.Effect of low temperature stress on root vigor and osmotic adjustment matter in Muskmelon Seedlings.J Shihezi Univ: Nat Sci, 2008, 26(5): 583-586.(in Chinese with English abstract)
[22]	Bohnert H J, Nelson D E, Jensen R G. Adaptations to environmental stresses. Plant Cell, 1995(7): 1099-1111.
[23]	Noctor G, Foyer C H.Ascorbate and glutathione: Keeping active oxygen under control.Annu Rev Plant Physial Plant Mol Biol, 1998, 49: 249-279.
[24]	Quartacci M F, Navarilzzo F.Water stress and free radical mediated changes in sunflower seeding.Plant Physiol, 1991, 139(5): 621-625.
[25]	贺丹, 毛仓仓, 陈颖, 等. H2O2对牡丹愈伤组织分化及抗氧化酶活性的影响. 湖北农业科学, 2015, 54(1): 122-125.
	He D, Mao C C, Chen Y, et al.Effects of on callus differentiation and antioxidant enzymes activity of peony.Hubei Agric Sci, 2015, 54(1): 122-125. (in Chinese with English abstract)
[26]	宋建, 乐明凯, 符冠富, 等. 水稻高温胁迫伤害机理研究进展. 中国稻米, 2009, 6: 8-14.
	Song J, Le M K, Fu G F, et al.Progress in the study of damage mechanism of rice under high temperaturestress.China Rice, 2009, 6: 8-14. (in Chinese)
[27]	杨春祥, 李宪利, 高东升, 等. 低温胁迫对油桃花器官膜脂过氧化和保护酶活性的影响. 果树学报, 2005, 22(1): 69-71.
	Yang C X, Li X L, Gao D S, et al.Effects of chilling stress on membrane lipid peroxidation and activities of protective enzymes in nectarine flower organs.J Fruit Sci, 2005, 22(1): 69-71. (in Chinese with English abstract)
[28]	李训贞, 梁满中, 周广洽, 等. 水稻开花时的环境条件对花粉活力和结实的影响. 作物学报, 2008, 28(3): 417-420.
	Li X Z, Liang M Z, Zhou G Q, et al Effect of environment condition on pollen vigor and seed set during flowing time of rice.Acta Agron Sin, 2008, 28(3): 417-420. (in Chinese with English abstract)
[29]	Zhang C X, Fu G F, Yang X Q, et al.Heat stress effects are stronger on spikelets than on flag leaves in rice due to differences in dissipation capacity.J Agron Crop Sci, 2015, doi: 10.1111/jac, 12138.
[30]	陶龙兴, 谈惠娟, 王熹, 等. 超级杂交稻国稻6号对开花结实期高温热害的反应. 中国水稻科学, 2007, 21(5): 518-524.
	Tao L X, Tan H J, Wang X, et al.Effects of high temperature stress on sperhybird rice Guodao 6 during flowering and filling phases.Chin J Rice Sci, 2007, 21(5): 518-524. (in Chinese with English abstract)
[31]	符冠富, 宋健, 廖西元, 等. 中国常用水稻保持系及恢复系开花灌浆期耐热性评价. 中国水稻科学, 2011, 25(5): 495-500.
	Fu G F, Song J, Liao X Y, et al.Thermal resistance of common rice maintainer and mestorer lines to high temperature stress during flowering and early grain filling stages.Chin J Rice Sci, 2011, 25(5): 495-500. (in Chinese with English abstract)
[32]	Liao J L, Zhang H Y, Shao X L, et al.Identificantion for heat tolerance in backcross recombinant screening of backcross introgression lines with heat tolerance at milky stage in rice.Rice Sci, 2011, 18: 279-286.
[33]	李永庚, 于振文, 张秀杰, 等. 小麦产量与品质对灌浆不同阶段高温胁迫的响应. 植物生态学报, 2005, 29(3): 461-466.
	Li Y K, Yu Z W, Zhang X J, et al.Response of yield and quality of wheat to heat stress at different grain filling stages.Acta Phytoecol Sin, 2005, 29(3): 461-466. (in Chinese with English abstract)
[34]	薛建鹏, 王秋玉, 任欢. 干旱胁迫下白桦主要生理指标的变化及相互关系. 东北林业大学学报, 2007, 35(8): 12-15.
	Xue J P, Wang Q Y, Ren H.Drought stress-induced responses of physiological indices of Betula platyphylla. J Northeast For Univ, 2007, 35(8): 12-15(in Chinese with English abstract).
[35]	杨升, 张华新, 张丽. 植物耐盐生理生化指标及耐盐植物筛选综述. 西北林学院学报, 2010, 25(3): 59-65.
	Yang S, Zhang H X, Zhang L.Physiological and biochemical indices of salt tolerance and scanning of salt-tolerance plant: A review.J Northwest For Univ, 2010, 25(3): 59-65. (in Chinese with English abstract)
[36]	张桂莲, 张顺堂, 肖浪涛, 等. 抽穗开花期高温胁迫对水稻花药、花粉粒及柱头生理特性的影响. 中国水稻科学, 2014, 28(2): 155-166.
	Zhang G L, Zhang S T, Xiao L T, et al.Effect of high temperature stress on physiological characterstics of anther, pollen and stigma of rice during head-flowering stage.Chin J Rice Sci, 2014, 28(2): 155-166. (in Chinese with English abstract)
[37]	李萍萍,程高峰,张佳华,等. 高温对水稻抽穗扬花期生理特性的影响. 江苏大学学报,2010,31(2): 125-130.
	Li P P, Cheng G F, Zhang J H, et al.Effects of high temperature stress on physiological characteristics of rice during heading and flowering period.J Jiangsu Univ,2010, 31(2): 125-130. (in Chinese with English abstract)
[38]	Anjum S A, Xie X Y, Wang L C, et al.Morphological, physiological and biochemical responses of plants to drought stress.Afric J Agric Res, 2011, 6(9): 2026-2032.
[39]	Pongprayoon W, Chaum S, Pichakum A, et al.Proline profiles in aromatic rice cultivars photoautotrophically grown in responses to salt stress.Inter J Bot,2008,4(3): 276-282.