Chinese Journal OF Rice Science ›› 2015, Vol. 29 ›› Issue (1): 35-44.DOI: 10.3969/j.issn.1001-7216.2015.01.005
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Hong-min ZHOU, Hua-dun WANG, Rui SUN, Wen-xia PEI, Xue-neng WU, Yue CAO, Ya-fei SUN, Guo-hua XU, Shu-bin SUN*()
Received:
2014-01-09
Revised:
2014-03-08
Online:
2015-01-10
Published:
2015-01-10
Contact:
Shu-bin SUN
周红敏, 王化敦, 孙瑞, 裴文霞, 吴学能, 曹越, 孙雅菲, 徐国华, 孙淑斌*()
通讯作者:
孙淑斌
基金资助:
CLC Number:
Hong-min ZHOU, Hua-dun WANG, Rui SUN, Wen-xia PEI, Xue-neng WU, Yue CAO, Ya-fei SUN, Guo-hua XU, Shu-bin SUN. OsSIZ1 Regulates the Development and Architecture of the Roots under Phosphate Starvation Conditions in Rice[J]. Chinese Journal OF Rice Science, 2015, 29(1): 35-44.
周红敏, 王化敦, 孙瑞, 裴文霞, 吴学能, 曹越, 孙雅菲, 徐国华, 孙淑斌. 水稻SUMO化E3连接酶SIZ1调控缺磷条件下根的发育和根构型形成[J]. 中国水稻科学, 2015, 29(1): 35-44.
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URL: http://www.ricesci.cn/EN/10.3969/j.issn.1001-7216.2015.01.005
基因 Gene | 引物序列 Primer sequences (5'-3') |
---|---|
OsActin | F: GGAACTGGTATGGTCAAGGC |
R: AGTCTCATGGATAACCGCAG | |
OsPIN1a | F: TCATCTGGTCGCTCGTCTGC |
R: CGAACGTCGCCACCTTGTTC | |
OsPIN1b | F: TGCACCCTAGCATTCTCAGCA |
R: CCCTCCTCCCAAATTCTACTT | |
OsYUCCA1 | F: TCATCGGACGCCCTCAACGTCGC |
R: GGCAGAGCAAGATTATCAGTC | |
OsPIN2 | F:CAACACCTACTCCAGCCTC |
R:TGGACCAGTCAAGAACCTC |
Table 1 Primers used to amplify the OsYUCCA1 and OsPINs cDNA fragments.
基因 Gene | 引物序列 Primer sequences (5'-3') |
---|---|
OsActin | F: GGAACTGGTATGGTCAAGGC |
R: AGTCTCATGGATAACCGCAG | |
OsPIN1a | F: TCATCTGGTCGCTCGTCTGC |
R: CGAACGTCGCCACCTTGTTC | |
OsPIN1b | F: TGCACCCTAGCATTCTCAGCA |
R: CCCTCCTCCCAAATTCTACTT | |
OsYUCCA1 | F: TCATCGGACGCCCTCAACGTCGC |
R: GGCAGAGCAAGATTATCAGTC | |
OsPIN2 | F:CAACACCTACTCCAGCCTC |
R:TGGACCAGTCAAGAACCTC |
Fig.1. Growth performances of seminal roots of ossiz1 and wild type. A, Root phenotype of rice seedlings 7 d after germination, bar=1 cm; B, The root hair proliferation on the seminal roots of rice seedlings 7 d after germination, bar=2 cm; C,D, The length and the growth rate of seminal root of rice seedlings for 7 d after transfer to the nutrient solution. WT, Wild type.
Fig. 2. Root phenotypic analysis of wild type(WT) and ossiz1 seedlings in the Pi-sufficient(+P) and Pi-deficient(-P) solid culture medium. In A, Bar=1 cm; In C, Bar=2 cm.
Fig. 3. Root phenotype of wild type (WT) and ossiz1 seedlings under Pi-sufficient(+P) and Pi-deficient(-P) conditions. In A, Bar=1 cm; In C; Bar=2 cm.
Fig. 4. Statistical analysis of adventitious roots of ossiz1 and its wild type (WT) seedlings under Pi-sufficient (+P) and Pi-deficient (-P) conditions. A,Root scanning; B, Adventitious roots without large lateral root formation in clumps (a, c, e, g) and adventitious roots with large lateral root formation in clumps (b, d, f, h); C,The ratio of adventitious roots with large lateral root formation in clumps to the total adventitious roots.
Fig. 5. Total P concentration in the shoots and roots of ossiz1 and its wild type (WT) seedlings under Pi-sufficient (+P) and Pi-deficient (-P) conditions in hydroponics culture.
Fig. 6. Expression of auxin genes in roots of WT and ossiz1 in hydroponics experiment. A,Relative expression of auxin biosynthesis gene OsYUCCA1 subjected to +P and -P conditions for 2 weeks; B, Relative expression of auxin efflux transporters OsPINs subjected to +P and -P conditions for 2 weeks.
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