Improving Resistance to Rice Stripe Disease and Eating Quality of Wuyujing 3 by Pyramiding Stv-bi and Wx-mq

doi:10.3969/j.issn.1001G7216.2015.05.003

Abstract

Abstract:

The japonica rice variety Kantou 194 was used as the donor of the resistance gene Stv-bⁱ and the low-amylose content gene Wx-mq to improve the resistance and eating quality of Wuyujing 3 by backcross. In continuous backcross and selfcross, molecular markers linked with Stv-bⁱ and Wx-mq were utilized for genotypic detection in combination with resistance identification to rice stripe disease and agronomic trait selection. Finally, 10 lines with homozygous genotype Stv-bⁱStv-bⁱWx-mqWx-mq were obtained from BC₃F₄ generation. The results of comparative analysis indicated that with similar agronomic traits to the receptor parent Wuyujing 3, those lines had substantial improments in resistance, appearance and eating quality. By comprehensive evaluation, two excellent lines K01 and K04 were selected.

Key words: rice, rice stripe disease, eating quality, Stv-bi, Wx-mq, pyramiding breeding

摘要：

以日本优质粳稻关东194为条纹叶枯病抗性基因Stv-bⁱ和低直链淀粉含量基因Wx-mq的供体, 采用回交育种的策略, 改良武育粳3号的抗性和食味品质。在连续回交和自交的过程中, 利用与Stv-bⁱ和Wx-mq基因相关的分子标记进行跟踪检测, 并结合条纹叶枯病的田间抗性鉴定以及农艺性状的系统选择, 在BC₃F₄世代中筛选到10个具有Stv-bⁱStv-bⁱWx-mqWx-mq纯合基因型的改良系。性状分析表明, 这些品系保留了受体亲本武育粳3号农艺性状, 条纹叶枯病抗性得到明显改良, 同时,食味、外观品质也大幅提升。综合分析, 最终筛选出K01和K04两个优异品系。

关键词: 水稻, 条纹叶枯病, 食味品质, Stv-bi基因, Wx-mq基因, 聚合育种

CLC Number:

Tao CHEN, Hao WU, Ya-dong ZHANG, Zhen ZHU, Qing-yong ZHAO, Li-hui ZHOU, Shu YAO, Ling ZHAO, Xin YU, Chun-fang ZHAO, Cai-lin WANG. Improving Resistance to Rice Stripe Disease and Eating Quality of Wuyujing 3 by Pyramiding Stv-bⁱ and Wx-mq[J]. Chinese Journal OF Rice Science, 2015, 29(5): 467-474.

陈涛, 吴昊, 张亚东, 朱镇, 赵庆勇, 周丽慧, 姚姝, 赵凌, 于新, 赵春芳, 王才林. 聚合Stv-bⁱ和Wx-mq基因改良武育粳3号条纹叶枯病抗性和食味品质[J]. 中国水稻科学, 2015, 29(5): 467-474.

Figures/Tables 8

References 21

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品系或品种 Line (Variety)	条纹叶枯病发病率 Occurance of RSD/%		抗性水平 Resistance level
品系或品种 Line (Variety)	江苏南京 Nanjing,Jiangsu		江苏盐都 Yandu,Jiangsu
K01	0.00±0.00	0.20±0.20	高抗HR
K02	1.41±0.21	1.55±0.30	高抗HR
K03	1.15±0.31	1.48±0.32	高抗HR
K04	0.20±0.00	0.41±0.20	高抗HR
K05	0.26±0.23	0.94±0.23	高抗HR
K06	0.26±0.11	0.87±0.23	高抗HR
K07	2.73±0.50	3.74±0.51	高抗HR
K08	0.07±0.12	0.40±0.20	高抗HR
K09	0.88±0.31	1.48±0.31	高抗HR
K10	0.79±0.39	1.28±0.12	高抗HR
武育粳3 WYJ3	64.82±0.80	68.97±0.41	高感HS

品系或品种 Line (Variety)	条纹叶枯病发病率 Occurance of RSD/%		抗性水平 Resistance level
品系或品种 Line (Variety)	江苏南京 Nanjing,Jiangsu		江苏盐都 Yandu,Jiangsu
K01	0.00±0.00	0.20±0.20	高抗HR
K02	1.41±0.21	1.55±0.30	高抗HR
K03	1.15±0.31	1.48±0.32	高抗HR
K04	0.20±0.00	0.41±0.20	高抗HR
K05	0.26±0.23	0.94±0.23	高抗HR
K06	0.26±0.11	0.87±0.23	高抗HR
K07	2.73±0.50	3.74±0.51	高抗HR
K08	0.07±0.12	0.40±0.20	高抗HR
K09	0.88±0.31	1.48±0.31	高抗HR
K10	0.79±0.39	1.28±0.12	高抗HR
武育粳3 WYJ3	64.82±0.80	68.97±0.41	高感HS

品系或品种 Line (Variety)	株高 PH/cm	穗长 PL/cm	单株穗数 PP	单株实粒数 FGPL	单株颖花数 SPL	每穗实粒数 FGPA	每穗颖花数 SPA	结实率 SSR/%	千粒重 TGW/g	单株产量 GWP/g
K01	97.9	15.7	14.3	1397.9	1452.2	98.6	102.6	96.3	25.0	33.6
K02	99.4	15.0	14.6	1421.1	1475.3	97.2	101.0	96.2	24.7	32.7
K03	99.1	15.4	14.3	1289.6	1354.7	90.8	95.5	95.2	24.7	31.2
K04	99.8	15.9	14.9	1336.7	1383.6	89.3	92.6	96.5	24.9	32.3
K05	99.7	15.5	13.4	1249.0	1291.6	93.6	96.8	96.7	25.9	32.1
K06	101.5	15.3	13.9	1324.7	1372.3	95.3	98.8	96.4	25.5	33.7
K07	100.2	15.9	13.4	1288.7	1329.5	96.3	99.4	96.9	25.1	32.2
K08	100.2	15.1	14.0	1322.9	1390.8	94.7	99.6	95.1	25.5	32.8
K09	100.0	15.6	13.7	1292.0	1347.2	94.3	98.4	95.8	25.6	33.0
K10	99.5	15.8	14.7	1348.4	1391.1	92.0	95.0	96.7	24.7	33.2
武育粳3号 WYJ 3	98.6	15.6	13.6	1258.5	1325.4	93.6	98.6	95.0	25.9	32.8

品系或品种 Line (Variety)	株高 PH/cm	穗长 PL/cm	单株穗数 PP	单株实粒数 FGPL	单株颖花数 SPL	每穗实粒数 FGPA	每穗颖花数 SPA	结实率 SSR/%	千粒重 TGW/g	单株产量 GWP/g
K01	97.9	15.7	14.3	1397.9	1452.2	98.6	102.6	96.3	25.0	33.6
K02	99.4	15.0	14.6	1421.1	1475.3	97.2	101.0	96.2	24.7	32.7
K03	99.1	15.4	14.3	1289.6	1354.7	90.8	95.5	95.2	24.7	31.2
K04	99.8	15.9	14.9	1336.7	1383.6	89.3	92.6	96.5	24.9	32.3
K05	99.7	15.5	13.4	1249.0	1291.6	93.6	96.8	96.7	25.9	32.1
K06	101.5	15.3	13.9	1324.7	1372.3	95.3	98.8	96.4	25.5	33.7
K07	100.2	15.9	13.4	1288.7	1329.5	96.3	99.4	96.9	25.1	32.2
K08	100.2	15.1	14.0	1322.9	1390.8	94.7	99.6	95.1	25.5	32.8
K09	100.0	15.6	13.7	1292.0	1347.2	94.3	98.4	95.8	25.6	33.0
K10	99.5	15.8	14.7	1348.4	1391.1	92.0	95.0	96.7	24.7	33.2
武育粳3号 WYJ 3	98.6	15.6	13.6	1258.5	1325.4	93.6	98.6	95.0	25.9	32.8

品系或品种 Line (Variety)	江苏溧水 Lishui,Jiangsu			江苏泰州 Tai zhou,Jiangsu			两地平均 Average
品系或品种 Line (Variety)	产量 Yield /(kg·hm^-2)		位次 Ranking		产量 Yield/(kg·hm^-2)	位次 Order	产量 Yield/(kg·hm^-2)	位次 Ranking
K01	7 543.5	5	10 680.0	4		9 111.8		3
K02	7 362.0	10	10 740.0	3		9 051.0		5
K03	7 335.0	11	10 132.5	10		8 733.8		11
K04	7 422.0	9	10 822.5	2		9 122.3		2
K05	7 485.0	6	10 035.0	11		8 760.0		10
K06	7 755.0	2	10 732.5	1		9 243.8		1
K07	7 887.0	1	10 155.0	9		9 021.0		6
K08	7 618.5	4	10 305.0	7		8 961.8		8
K09	7 740.0	3	10 432.5	6		9 086.3		4
K10	7 437.0	7	10 545.0	5		8 991.0		7
武育粳3号 WYJ3	7 429.5	8	10 257.0	8		8 843.3		9

Improving Resistance to Rice Stripe Disease and Eating Quality of Wuyujing 3 by Pyramiding Stv-bⁱ and Wx-mq

聚合Stv-bⁱ和Wx-mq基因改良武育粳3号条纹叶枯病抗性和食味品质

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Figures/Tables 8

References 21

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品系或品种 Line (Variety)	碾米品质 Milling quality			外观品质 Appearance quality						食味品质 Eating quality
品系或品种 Line (Variety)	糙米率 Brown rice rate /%		精米率 Milled rice rate /%		整精米率 Head milled rice rate/%	粒长 Grain length/mm	长宽比 Length-width ratio	垩白粒率 Chalky rice rate /%	垩白度 Chalkiness degree /%	胶稠度 Gel consistency	碱消值 Alkali value	直链淀粉含量 Amylose content /%
K01	79.5	71.8	68.5	4.72	1.65	8.5^**	1.2^**		80.8^**		5.8	8.2^**
K02	78.7	70.2	65.8	4.46	1.65	24.2^**	4.2^**		81.2^**		5.8	8.2^**
K03	78.7	70.9	68.0	4.53	1.68	13.7^**	2.9^**		79.7^**		5.8	7.5^**
K04	79.8	71.9	68.1	4.53	1.66	14.7^**	2.9^**		80.1^**		5.7	7.5^**
K05	80.3	72.5	68.6	4.98	1.63	23.9^**	4.0^**		81.1^**		5.8	7.6^**
K06	80.3	71.8	68.7	4.95	1.63	14.1^**	2.1^**		81.2^**		5.8	7.6^**
K07	79.2	71.2	68.5	4.74	1.69	13.7^**	2.9^**		81.5^**		5.8	8.1^**
K08	79.5	71.2	68.2	4.93	1.66	12.2^**	2.2^**		81.7^**		5.7	8.7^**
K09	79.7	72.4	69.2	4.93	1.67	18.0^**	4.0^**		81.7^**		5.7	8.9^**
K10	78.7	71.6	69.1	4.70	1.66	21.8^**	4.5^**		82.2^**		5.8	8.9^**
武育粳3号 WYJ3	78.8	70.1	67.1	4.99	1.61	45.2	9.2		75.8		5.8	14.2

品系或品种 Line (Variety)	气味 Odor	外观 Appearance	口感 Palatability	味道 Fragrance	综合评价 Comprehensive score	食味值 Taste value
K01	0.10	0.80	1.40^**	0.90^*	1.50^**	0.96^**
K02	0.20	1.10^*	1.30^*	1.20^**	1.50^**	1.13^**
K03	0.10	0.40	0.70	0.50	0.80	0.51
K04	0.10	0.90^*	0.90	0.70	0.90	0.74
K05	0.10	0.70	0.60	0.60	0.70	0.58
K06	0.00	0.50	1.00	1.00^*	1.38^*	0.84^*
K07	0.10	1.20^**	1.70^**	1.60^**	1.90^**	1.42^**
K08	0.10	1.10^*	1.40^**	0.90^*	1.50^**	1.02^**
K09	0.00	0.90^*	1.30^*	1.30^**	1.40^*	1.10^**
K10	0.20	0.90^*	1.30^*	1.20^**	1.20^*	1.16^**
武育粳3号 WYJ 3	0.00	0.00	0.00	0.00	0.00	0.00

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