中国水稻科学

中国水稻科学

• 研究报告 • 上一篇    下一篇

栽培稻种间近等基因系杂种育性遗传及其基因定位

陶大云1; 徐鹏1; 李静1; 杨友琼1; 周家武1; 胡凤益1; Monty P. JONES 2;   

  1. 1云南省农业科学院 粮食作物研究所， 云南 昆明 650205； 2西非稻作发展协会， 科特迪瓦 布瓦凯 2551
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2003-01-10 发布日期:2003-01-10

Hybrid Sterility Inheritance and Mapping of Sterile Genes among Near-Isogenic Lines Derived from Interspecific Hybrid Between Cultivated Rice Species Oryza sativa and O. glaberrima

TAO Da-yun 1; XU Peng 1; LI Jing 1; YANG You-qiong 1; ZHOU Jia-wu 1; HU Feng-yi 1; Monty P. JONES 2   

  1. （1Food Crops Research Institute; Yunnan Academy of Agricultural Sciences; Kunming 650205; China; 2West Africa Rice Development Association; 01 B. P. 2551 Bouake 01; Cote D Ivoire）;
  • Received:1900-01-01 Revised:1900-01-01 Online:2003-01-10 Published:2003-01-10

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摘要： 用粳型亚洲栽培稻品种WAB56-104与来自种间回交组合WAB56-/104/CG14∥WAB56-104///WAB56-104的3个栽培稻种间近等基因系杂交、回交，对3个BC1F1群体的花粉及小穗育性遗传研究表明，育性遗传符合单位点孢子体-配子体互作模型，来自非洲栽培稻的不育基因在与相同位点的亚洲栽培稻等位基因互作时，导致携带亚洲栽培稻等位基因的雌雄配子败育，而形成花粉及小穗的半不育，但本研究的3个组合中，并未导致所有携带亚洲栽培稻等位基因的雌配子完全败育，其作用介于配子消除与花粉灭杀之间。用微卫星标记对这3个群体的育性基因定位表明，它们都位于第6染色体短臂末端，但WAB450-2、WAB450-8携带的不育位点与紧密连锁的微卫星标记RM190、RM133共分离，且很可能与S1等位，而WAB450-7的不育基因则与位于相邻区间的RM253呈松散连锁，可能与WAB450-2、WAB450-8的育性位点不同。

关键词: 亚洲栽培稻, 非洲栽培稻, 种间杂种, 育性, 基因定位

Abstract: Three combinations of WAB56-104/WAB450-2∥WAB56-104, WAB56-104/WAB450-7∥WAB56-104, and WAB56-104/WAB450-8∥WAB56-104 were used to study inheritance of pollen and seed setting rate in Sanya, Hainan Province in the early season rice (March-July, 2001). WAB56-104 and CG14 were upland japonica rice (Oryza sativa) and O. glaberrima, respectively. WAB450-2, WAB450-7, and WAB450-8 were derived from interspecific BC2 cross WAB56-104/CG14∥WAB56-104///WAB56-104. Results indicated that semi sterility of pollen and spikelet could be explained by one locus sporo gametophytic interaction model. However, elimination of female gametes was incomplete. Gene mapping with SSR markers showed that sterile gene in WAB450-2 and WAB450-8 was located on short arm of chromosome 6, cosegregated with closely linked SSR markers RM190 and RM133 respectively, which was high possible allelic to S1 locus. But sterile gene in WAB450-7 was loosely linked to RM253, which indicated that the sterile gene locus in WAB450-7 might be different from that in WAB450-2 and WAB450-8.

Key words: Oryza sativa, O. glaberrima, interspecific hybrid, sterility, gene mapping