中国水稻科学 ›› 2023, Vol. 37 ›› Issue (1): 45-54.DOI: 10.16819/j.1001-7216.2023.220109

• 研究报告 • 上一篇    下一篇

水稻光温敏雄性不育突变体tms3650的鉴定和基因定位

唐杰1(), 龙湍2, 吴春瑜1, 李新鹏1, 曾翔1, 吴永忠1, 黄培劲1   

  1. 1海南波莲水稻基因科技有限公司, 海口 510125
    2海南大学 热带作物学院, 海口 510125
  • 收稿日期:2022-01-09 修回日期:2022-03-22 出版日期:2023-01-10 发布日期:2023-01-10
  • 通讯作者: 唐杰
  • 基金资助:
    中国水稻所水稻生物学国家重点实验室开放课题资助项目(20190203)

Identification and Gene Mapping of a New Photo-thermo-sensitive Male Sterile Mutant tms3650 in Rice

TANG Jie1(), LONG Tuan2, WU Chunyu1, LI Xinpeng1, ZENG Xiang1, WU Yongzhong1, HUANG Peijin1   

  1. 1Hainan Bolian Rice Gene Technology Co., Ltd., Haikou 570125, China
    2College of Tropical Crops, Hainan University, Haikou 570125, China
  • Received:2022-01-09 Revised:2022-03-22 Online:2023-01-10 Published:2023-01-10
  • Contact: TANG Jie

摘要: 目的 雄性不育是水稻杂种优势利用的基础。为进一步解析其调控机制,对一个雄性不育突变体进行了鉴定。方法 利用60Co-γ对籼稻品种93-11进行辐射诱变,从其后代中鉴定到一个雄性不育突变体tms3650。将籼稻明恢63作父本同突变体tms3650杂交构建F2和F3群体,采用图位克隆的方法对目的基因进行精细定位。结果 tms3650突变体其他农艺性状与野生型一致,但花药白绿且瘦小,花粉不能被1%碘-碘化钾溶液染成蓝黑色,穗子包颈,抽穗期延迟。遗传分析表明该突变体表型受一对隐性核基因控制。精细定位结果表明基因位于第3染色体长臂SSR标记RM15927和RM15934之间135.25 kb距离内,且与RM15931标记共分离。陵水冬季南繁鉴定发现,该突变体育性受光温环境影响,说明tms3650突变体的雄性育性在短日低温条件下发生了转育,是一个光温敏雄性不育突变体。结论 通过将定位位点与已报道的雄性不育基因比较,发现tms3650是一个新的基因位点,暂命名为TMS3650

关键词: 水稻, 光温敏雄性不育, 基因定位

Abstract:

【Objective】 Male sterility is the basis of rice heterosis utilization. To further analyze the regulation mechanism, a male sterility mutant was identified.【Method】 The tms3650 mutant was isolated from the mutant library of 60Co-γ-treated indica cultivar 93-11. The indica rice Minghui 63 was crossed with the mutant tms3650 as the male parent to construct F2 and F3 populations, and the target gene was fine-mapped by map-based cloning. 【Result】 The other agronomic traits of tms3650 were the same as those of the wild type, but the anthers of the mutant were white-green and thin, the pollen could not be stained blue-black by 1% iodine-potassium iodide solution with panicle-enclosure and delayed heading stage. Genetic analysis indicated that the mutant phenotype was controlled by a pair of recessive nuclear genes. The results of fine mapping showed that the gene was located within a distance of 135.25 kb between the SSR markers RM15927 and RM15934 on the long arm of chromosome 3, and co-segregated with the RM15931 marker. The identification of southern breeding in Lingshui in winter revealed that the fertility of mutant was affected by light and temperature, indicating that the tms3650 was a photo-thermo-sensitive male sterile mutant. 【Conclusion】 By comparing with the reported male sterility genes, the tms3650 was controlled by a new gene locus, TMS3650.

Key words: rice, photo-thermo-sensitive male sterility, gene mapping