Effects of Plant Growth-promoting Rhizobacteria(PGPR) on Physiological Characteristics of Rice Under Drought Stress

doi:10.16819/j.1001-7216.2018.7123

Abstract

Abstract:

【Objective】This study aims to reveal the effects of plant growth-promoting rhizobacteria Bacillus cereus F06 on physiological characteristics of the rice Shanyou 63 under different drought stress treatments(light, moderate, severe and a control).【Method】The combined effects of F06 inoculation and various levels of drought stress on the photosynthetic characteristics, chlorophyll fluorescence parameters, cytokinin and abscisic acid(ABA) concentrations, relative water content,and relative electrolyte leakage were studied in a pot experiment. 【Result】The results showed that P_n and g_s decreased with increasing drought stress. However, F06 inoculation significantly slowed down the decrease of P_n and g_s under drought stress compared with non-inoculation(NP) treatment, P_n and g_s increased by 7.67%, 12.97%, 18.14% and 11.51%, 16.63% and 17.07%, respectively. With the intensifying drougnt stress, it followed an increasing trend. F06 inoculation inhibited the decrease of F_v/ F_m and q_P and the increase of F_o, NPQ and improved the light conversion efficiency of rice leaves significantly under drought stress. Under the drought stress, B.cereus F06 inoculation could reduce the change amplitude of leaf water potential, relative water content and relative conductivity. However, F06 inoculation could not reverse the decrease trend of leaf water potential,relative water content and relative conductivity. F06 inoculation also significantly depressed the pigment decomposition or reduction. Although no significant increase was observed under well-watered conditions, drought significantly reduced the cytokinin(CTK) content in leaves and roots of rice, and increased ABA content in leaves, but F06 inoculation significantly increased the cytokinin content of drought-stressed leaves and roots Shanyou 63. 【Conclusion】In conclusion,inoculation of PGPR could reduce the decomposition or loss of photosynthetic pigments and improve the photosynthetic rate,showing real potential for practical use in arid environments as a drought stress inhibitor．

Key words: Key Words: drought stress, rice, plant growth-promoting rhizobacteria(PGPR), physiological characteristics

摘要：

【目的】本研究旨在探究植物根际促生菌蜡状芽孢杆菌F06菌株对不同干旱胁迫下水稻汕优63生理特性的影响。【方法】在盆栽试验条件下,以水稻汕优63为种植材料,研究了轻度(LD)、中度(MD)、重度(SD)3个干旱强度下接种蜡样芽孢杆菌(Bacillus cereus)F06对水稻生理特征的影响。【结果】与正常水分管理相比,干旱胁迫(DS)下水稻叶片光合速率(P_n)和气孔导度(g_s)逐渐降低;而干旱胁迫下接种F06可显著减缓P_n和g_s下降,与不接种(NP)处理相比,P_n和g_s分别增加7.67%、12.97%、18.14%和11.51%、16.63%、17.07%,且呈现出随着干旱胁迫程度的提高,增幅增大的趋势。干旱胁迫下水稻叶片初始荧光(F_o)、非荧光淬灭系数(NPQ)显著上升,最大光化学效率(F_v/F_m)、光化学猝灭系数(q_P)显著下降;而干旱胁迫下接种F06可显著抑制F_o、NPQ升高和F_v/F_m、q_P降低,明显改善水稻叶片光能转换效率。干旱胁迫下接种F06虽然不能改变叶片水势、相对含水量和相对电导率的变化趋势,但可以有效降低其变幅。正常水分处理下接种F06虽然没有增加光合色素含量,但干旱环境下显著抑制了光合色素的分解或降低。干旱显著降低了水稻叶片和根系细胞分裂素(CTK)含量,增加了叶片中脱落酸(ABA)的含量;在干旱胁迫下,接种F06可显著提高叶片和根系中CTK的含量。【结论】由此可见,干旱生境下接种F06,可调节植物体内的激素含量,减少干旱胁迫下光合色素的分解或流失,提高光合速率,增强水稻在干旱环境中的适应能力。

关键词: 干旱胁迫, 水稻, 根际促生细菌, 生理特性

CLC Number:

Su CHEN, Jiankun XIE, Wenxin HUANG, Dengyun CHEN, Xiaojian PENG, Xueqin FU. Effects of Plant Growth-promoting Rhizobacteria(PGPR) on Physiological Characteristics of Rice Under Drought Stress[J]. Chinese Journal OF Rice Science, 2018, 32(5): 485-492.

陈苏, 谢建坤, 黄文新, 陈登云, 彭晓剑, 付学琴. 根际促生细菌对干旱胁迫下水稻生理特性的影响[J]. 中国水稻科学, 2018, 32(5): 485-492.

Figures/Tables 5

References 30

[1]	丁雷, 李英瑞, 李勇, 沈其荣, 郭世伟.梯度干旱胁迫对水稻叶片光合和水分状况的影响. 中国水稻科学, 2014, 28(1): 65-70.
	Ding L, Li Y R, Li Y, Shen Q R, Guo S W.Effects of drought stress on photosynthesis and water status of rice leaves.Chin J Rice Sci, 2014, 28(1): 65-70.(in Chinese with English abstract)
[2]	胡继杰, 朱练峰, 钟楚, 林育炯, 张均华, 曹小闯, 禹盛苗, James A B, 金千瑜. 增氧模式对水稻光合特性及产量的影响. 中国水稻科学, 2017, 31(3): 278-287.
	Hu J J, Zhu L F, Zhong C, Lin Y J, Zhang J H, Cao X C, Yu S M. Allen B J, Jin Q Y.Effects of aeration methods on photosynthetic characteristics and yield of rice.Chin J Rice Sci, 2017, 31(3): 278-287.(in Chinese with English abstract)
[3]	康贻军, 程洁, 梅丽娟, 胡健, 朴哲, 殷士. 植物根际促生菌作用机制研究进展. 应用生态学报, 2010, 21( 1): 232-238.
	Kang Y J, Cheng J, Mei L J, Hu J, Piao Z, Yin S.Action mechanisms of plant growth promoting rhizobacterial (PGPR).Chin J Appl Ecol, 2010, 21(1): 232-238. (in Chinese with English abstract)
[4]	Abbasi M K, Sharif S, Kazmi M, Sultan T, Aslam M.Isolation of plant growth promoting rhizobacteria from wheat rhizosphere and their effect on improving growth, yield and nutrient uptake of plants.Plant Biosys, 2011, 145(1): 159-168.
[5]	Carvalhais L C, Dennis P G, Fedoseyenko D, Hajirezaei M R, Borriss R, von Wirén N V. Root exudation of sugars, amino acids and organic acids by maize as affected by nitrogen, phosphorus, potassium, and iron deficiency.J Plant Nut Soil Sci, 2011, 174(1): 3-11.
[6]	Kasim W A, Osman M E, Omar M N, El-Daim I A A, Be jais, Meijer J. Control of drought stress in wheat using plant-growth-promoting bacteria.J Plant Growth Reg, 2013, 32(1): 122-130.
[7]	刘方春, 马海林, 马丙尧, 杜振宇, 井大炜, 邢尚军. 干旱环境下接种根际促生细菌对核桃苗光合特性的影响. 林业科学, 2015, 51(7): 84-90.
	Liu F C, Ma H L, Ma B Y, Du Z Y, Jing D W, Xing S G.Effect of plant growth-promoting rhizobacteria on photosynthetic characteristics in walnut seedlings under drought stress.Sci Sil Sin, 2015, 51(7): 84-90. (in Chinese with English abstract)
[8]	郭贵华, 刘海艳, 李刚华, 刘明, 李岩, 王绍华, 刘正辉, 唐设, 丁艳锋. ABA 缓解水稻孕穗期干旱胁迫生理特性的分析. 中国农业科学, 2014, 47(22): 4380-4391.
	Guo G H, Liu H Y, Li G H, Liu M, Li Y, Wang S H, Liu Z H, Tang S, Ding Y F.Analysis of physiological characteristics about ABA alleviating rice booting stage drought stress.Sci Agric Sin, 2014, 47(22): 4380-4391. (in Chinese with English abstract)
[9]	Arkhipova T N, Prinsen E, Veselov S U, Martinenko E V, Melentiev A I.Cytokinin producing bacteria enhance plant growth in drying soil.Plant Soil, 2007, 292: 305-315.
[10]	Kasim W A, Osman M E, Omar M N, El-Daim I A A, Bejai S, Meijer J. Control of drought stress in wheat using plant-growth-promoting bacteria.J Plant Growth Reg, 2013, 32(1): 122-130.
[11]	Huang M, Guo Z.Responses of antioxidative system to chilling stress in two rice cultivars differing in sensitivity.Biol Plant, 2005, 49(1): 81-84.
[12]	欧立军, 陈波, 邹学校. 干旱对辣椒光合作用及相关生理特性的影响. 生态学报, 2012, 32(8): 2612-2619.
	Ou L J, Chen B, Zou X X.Effects of drought stress on photosynthesis and associated physiological characters of pepper. Acta Ecol Sin, 2012, 32(8): 2612-2619. (in Chinese with English abstract)
[13]	Guerfel M, Baccouri O, Boujnah D, Chaibi W, Zarrouk M.Impacts of water stress on gas exchange, water relations, chlorophyll content and leaf structure in the two main Tunisian olive (Olea europaea L.) cultivars. Sci Hort, 2009, 119(3): 257-263.
[14]	Johnson J D, Tognetti R, Paris P.Water relations and gas exchange in poplar and willow under water stress and elevated atmospheric CO2.Physiol Plant, 2002, 115(1): 93-100.
[15]	邵在胜, 赵轶鹏, 宋琪玲, 贾一磊, 王云霞, 杨连新, 王余龙. 大气CO2和O3浓度升高对水稻汕优63叶片光合作用的影响. 中国生态农业学报, 2014, 22(4): 422-429.
	Shao Z S, Zhao Y P, Song Q L, Jia Y L, Wang Y X, Yang L X, Wang Y L.Impact of elevated atmospheric carbon dioxide and ozone concentrations on leaf photosynthesis of ‘Shanyou 63’ hybrid rice. Chin J Eco-Agric, 2014, 22(4): 422-429. (in Chinese with English abstract)
[16]	Lichtenthaler H K.Chlorophylls and carotenoids: pigments of photosynthetic biomembranes.Methods Enzymol, 1987, 148: 349-382.
[17]	刘方春, 马海林, 杜振宇, 马丙尧, 井大炜, 邢尚军. 金银花容器苗对干旱胁迫下接种根际促生细菌的生理响应. 生态学报, 2015, 35(21): 7003-7010.
	Liu F C, Ma H L, Du Z Y, Ma B Y, Jing D W, Xing S G.Physiological responses of Lonicera japonica container seedlings to plant growth-promoting rhizobacteria inoculation under drought stress.Acta Ecol Sin, 2015, 35(21): 7003-7010. (in Chinese with English abstract)
[18]	刘海艳, 杨丽洁, 丁艳锋, 李刚华, 王绍华, 刘正辉, 唐设, 刘仁梅, 蒋卫红. NO 对水稻孕穗期干旱胁迫下叶片光合及产量的影响. 南京农业大学学报, 2017, 40(2): 195-202.
	Liu H Y, Yang L J, Ding Y F, Li G G, Wang S H, Liu Z H, Tang S, Liu R M, Jiang W H.Effects of nitric oxide on photosynthesis and yield of rice under drought stress at booting stage. J Nanjing Agric Univ, 2017, 40(2): 195-202. (in Chinese with English abstract)
[19]	Bartošková H, Komenda J, Nauš J.Functional changes of photosystemⅡ in the mossRhizomnium punctatum(Hedw.) induced by different rates of dark desiccation. J Plant Physiol, 1999, 154(5) : 597-604.
[20]	张金政, 张起源, 孙国峰, 何卿, 李晓东, 刘洪章. 干旱胁迫及复水对玉簪生长和光合作用的影响. 草业学报, 2014, 23(1): 167-176.
	Zhang J Z, Zhang Q V, Sun G F, He Q, Li X D, Liu H Z.Effects of drought stress and re-watering on growth and photosynthesis of Hosta. Acta Pratacul Sin, 2014, 23(1): 167-176. (in Chinese with English abstract)
[21]	赵琴, 潘静, 曹兵, 宋丽华. 气温升高与干旱胁迫对宁夏枸杞光合作用的影响. 生态学报, 2015, 35(18): 6016-6022.
	Zhao Q, Pan J, Cao B, Song L H.Effects of elevated temperature and drought stress on photosynthesis of Lycium barbarum. Acta Ecol Sin, 2015, 35(18): 6016-6022. (in Chinese with English abstract)
[22]	Brugiere N, Jiao S, Hantke S.Cytokinin oxidase gene expression in maize is localized to the vasculature, and is induced by cytokinins, abscisic acid, and abiotic stress.Plant Physio1, 2003, 132: 1228-1240.
[23]	李长宁, Manoj K S, 农倩, 李杨瑞. 水分胁迫下外源 ABA 提高甘蔗抗旱性的作用机制. 作物学报, 2010, 36(5): 863-870.
	Li C N, Manoj K S, Nong Q, Li Y R.Mechanism of tolerance to drought in sugarcane plant enhanced by foliage dressing of abscisic acid under water stress. Acta Agron Sin, 2010, 36(5): 863-870. (in Chinese with English abstract)
[24]	Kwak K S, Lijima M, Yamauchi A.Changes with aging of endogenous abscisic acid and zeatin riboside in the root system of rice.Jpn J Crop Sci, 1996, 65: 686-692.
[25]	Dodd I C, Davies W J.The relationship between leaf growth and ABA accumulation in the grass leaf elongation zone.J Exp Bot, 1996, 45: 1471-1478.
[26]	王玮, 张枫, 李德全,. 外源ABA 对渗透胁迫下玉米幼苗根系渗透调节的影响. 作物学报, 2002, 28(1): 121-126.
	Wang W, Zhang F, Li D Q.The effects of exogenous ABA on osmotic adjustment in maize roots under osmotic stress.Acta Agron Sin, 2002, 28(1): 121-126. (in Chinese with English abstract)
[27]	阮英慧, 董守坤, 刘丽君, 孙聪姝, 王立彬, 郭茜茜, 盖志佳. 干旱胁迫下外源脱落酸对大豆花期生理特性的影响. 大豆科学, 2012, 31(3): 385-389.
	Ruan Y H, Dong S K, Liu L J, Sun C S, Wang L B, Guo Q Q, Gai Z J.Effects of exogenous abscisic acid on physiological characteristics in soybean flowering under drought stress. Soyb Sci, 2012, 31(3): 385-389. (in Chinese with English abstract)
[28]	Heckenberger U, Schurr U, Schulze E D.Stomatal response to ABA fed into the xylem of intactHeliarthus annuus(L) Plant. J Exp Bot, 1996, 47: 1405-1412.
[29]	Liang J, Zhang J, Wong M H.How do roots control xylem sap ABA concentration in response to soil drying?Plant Cell Physiol, 1997, 38: 10-16.
[30]	周宇飞, 王德权, 陆樟镳, 王娜, 王艺陶, 李丰先, 许文娟, 黄瑞冬. 干旱胁迫对持绿性高粱光合特性和内源激素ABA、CTK 含量的影响. 中国农业科学, 2014, 47(4): 655-663.
	Zhou Y F, Wang D Q, Lu Z B, Wang N, Wang Y T, Li F X, Xu W J, Huang R D.Effects of drought stress on photosynthetic characteristics and endogenous hormone ABA and CTK contents in green-stayed sorghum.Sci Agric Sin, 2014, 47(4): 655-663. (in Chinese with English abstract)

干旱处理 Drought stress	光合速率 Photosynthetic rate/(µmol·m^-2s^-1)		气孔导度 Stomatal conductance/(mmol·m^-2s^-1)		胞间CO₂浓度 Intercellular CO₂ concentration/(µmol·mol^-1)
干旱处理 Drought stress	NP	F06	NP	F06	NP	F06
CK	27.25±1.26 a	28.71±2.84 a	256.69±11.54 a	283.32±12.67 a	227.18±29.89 bc	229.12±14.20 b
LD	25.94±2.02 ab	27.93±1.16 a	234.35±18.90 a	261.33±10.18 a	191.78±16.34 c	216.05±11.32 b
MD	23.06±1.37 b	26.05±3.12 ab	195.17±15.27 b	227.62±23.14 b	235.22±10.71 b	209.38±18.17 c
SD	18.91±1.18 c	22.34±2.41 b	165.28±20.77 c	193.49±15.63 c	268.53±21.44 a	252.68±8.95 a

干旱处理 Drought stress	光合速率 Photosynthetic rate/(µmol·m^-2s^-1)		气孔导度 Stomatal conductance/(mmol·m^-2s^-1)		胞间CO₂浓度 Intercellular CO₂ concentration/(µmol·mol^-1)
干旱处理 Drought stress	NP	F06	NP	F06	NP	F06
CK	27.25±1.26 a	28.71±2.84 a	256.69±11.54 a	283.32±12.67 a	227.18±29.89 bc	229.12±14.20 b
LD	25.94±2.02 ab	27.93±1.16 a	234.35±18.90 a	261.33±10.18 a	191.78±16.34 c	216.05±11.32 b
MD	23.06±1.37 b	26.05±3.12 ab	195.17±15.27 b	227.62±23.14 b	235.22±10.71 b	209.38±18.17 c
SD	18.91±1.18 c	22.34±2.41 b	165.28±20.77 c	193.49±15.63 c	268.53±21.44 a	252.68±8.95 a

干旱处理 Drought stress	初始荧光 PSⅡ original fluorescence(F_o)		最大光化学效率 Maximum photochemical efficiency(F_v/F_m)		光化学猝灭系数 Photochemical quenching Coefficient(q_P)		非光化学猝灭系数 Non-photochemical quenching Coefficient(NPQ)
干旱处理 Drought stress	NP	F06	NP	F06	NP	F06	NP	F06
CK	149.06±9.78 d	150.18±12.62 c	0.89±0.04 a	0.90±0.03 a	0.75±0.04 a	0.75±0.02 a	1.70±0.04 c	1.70±0.06 c
LD	162.19±12.78 c	154.44±19.41 bc	0.80±0.01 ab	0.86±0.02 ab	0.64±0.02 ab	0.63±0.02 b	1.77±0.05 b	1.76±0.05 c
MD	178.34±14.21 b	167.06±10.89 b	0.73±0.02 b	0.79±0.01 b	0.60±0.02 b	0.52±0.03 c	1.80±0.03 b	1.98±0.03 b
SD	192.18±16.74 a	178.03±11.36 a	0.52±0.01 c	0.61±0.02 c	0.51±0.01 c	0.38±0.00 d	2.06±0.07 a	2.24±0.06 a

干旱处理 Drought stress	初始荧光 PSⅡ original fluorescence(F_o)		最大光化学效率 Maximum photochemical efficiency(F_v/F_m)		光化学猝灭系数 Photochemical quenching Coefficient(q_P)		非光化学猝灭系数 Non-photochemical quenching Coefficient(NPQ)
干旱处理 Drought stress	NP	F06	NP	F06	NP	F06	NP	F06
CK	149.06±9.78 d	150.18±12.62 c	0.89±0.04 a	0.90±0.03 a	0.75±0.04 a	0.75±0.02 a	1.70±0.04 c	1.70±0.06 c
LD	162.19±12.78 c	154.44±19.41 bc	0.80±0.01 ab	0.86±0.02 ab	0.64±0.02 ab	0.63±0.02 b	1.77±0.05 b	1.76±0.05 c
MD	178.34±14.21 b	167.06±10.89 b	0.73±0.02 b	0.79±0.01 b	0.60±0.02 b	0.52±0.03 c	1.80±0.03 b	1.98±0.03 b
SD	192.18±16.74 a	178.03±11.36 a	0.52±0.01 c	0.61±0.02 c	0.51±0.01 c	0.38±0.00 d	2.06±0.07 a	2.24±0.06 a

干旱处理 Drought stress	水势 Water potential/MPa		相对含水量 Relative water content/%		相对电导率 Relative electrolyte leakage/%
干旱处理 Drought stress	NP	F06	NP	F06	NP	F06
CK	–0.91±0.03 a	–0.91±0.02 a	84.16±3.25 a	84.52±6.27 a	6.83±0.55 c	6.81±0.34 c
LD	–1.02±0.06 b	–0.93±0.05 a	79.52±5.29 b	82.80±4.22 a	7.14±0.35 c	6.92±0.41 c
MD	–1.15±0.06 c	–1.07±0.04 b	70.87±8.09 c	76.33±6.30 b	8.31±0.27 b	7.52±0.17 b
SD	–1.27±0.05 d	–1.16±0.04 c	61.82±5.77 d	67.27±7.06 c	9.45±0.67 a	8.48±0.19 a