Chinese Journal OF Rice Science ›› 2015, Vol. 29 ›› Issue (3): 319-326.DOI: 10.3969/j.issn.1001G7216.2015.03.012
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Hai-ping NI1,2, Qiu-fang XU2, Ying LAN2, Qing-qing CHEN2, Jin-feng ZHANG2, Yi-jun ZHOU1,2,*()
Received:
2014-12-01
Revised:
2015-01-29
Online:
2015-05-10
Published:
2015-05-10
Contact:
Yi-jun ZHOU
倪海平1,2, 徐秋芳2, 兰莹2, 陈晴晴2, 张金凤2, 周益军1,2,*()
通讯作者:
周益军
基金资助:
Hai-ping NI, Qiu-fang XU, Ying LAN, Qing-qing CHEN, Jin-feng ZHANG, Yi-jun ZHOU. Effect of RBSDV Infection on Transcriptional Expression of Abscisic Acid Metabolism Related Genes in Rice[J]. Chinese Journal OF Rice Science, 2015, 29(3): 319-326.
倪海平, 徐秋芳, 兰莹, 陈晴晴, 张金凤, 周益军. RBSDV侵染对水稻ABA代谢相关基因表达的影响[J]. 中国水稻科学, 2015, 29(3): 319-326.
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URL: http://www.ricesci.cn/EN/10.3969/j.issn.1001G7216.2015.03.012
基因名称 Gene name | 基因登录号 Accession No. | 引物序列 Primer sequence | 产物大小 Amplification length/bp |
---|---|---|---|
OsUBQ5 | AK061988 | F:5’-ACCACTTCGACCGCCACTACT-3’ | 69 |
R:5’-ACGCCTAAGCCTGCTGGTT-3’ | |||
OsZEP | AB050884 | F:5’-GGATGCCATTGAGTTTGGTT-3’ | 133 |
R:5’-TGGCTGACTGAAGTCTCTCG-3’ | |||
OsNCED1 | AY838897 | F:5’-CTCACCATGAAGTCCATGAGGCTT-3’ | 126 |
R:5’-GTTCTCGTAGTCTTGGTCTTGGCT-3’ | |||
OsNCED2 | AY838898 | F:5’-GGTATGGAAACGAGGATAGTGGTT-3’ | 100 |
R:5’-TGCTTATTGTTGTGCGAGAAGTTC-3’ | |||
OsNCED3 | AY838899 | F:5’-CCCCTCCCAAACCATCCAAACCGA-3’ | 132 |
R:5’-TGTGAGCATATCCTGGCGTCGTGA-3’ | |||
OsNCED4 | AY838900 | F:5’-TCGCGCTCAGCTACAATGTC-3’ | 88 |
R:5’-ATGTCGACGTCTCGGGACTT-3’ | |||
OsNCED5 | AY838901 | F:5’-ACATCCGAGCTCCTCGTCGTGAA-3’ | 184 |
R:5’-TTGGAAGGTGTTTTGGAATGAACCA-3’ | |||
OsABA8ox1 | NM_001054390 | F:5’-CCAAGAACCCCAACGTGTTC-3’ | 101 |
R:5’-CGGGCTGGACACCATCA-3’ | |||
OsABA8ox2 | NM_001068556 | F:5’-GCGAGACGCTCCAGCTCTA-3’ | 100 |
R:5’-GGGCACCCCAGCAGATTC-3’ | |||
OsABA8ox3 | NM_001069901 | F:5’-TCCAGCGACGAGGTTGAGTA-3’ | 100 |
R:5’-AACCATCTGTTTCCACACTGACA-3’ |
Table 1 Primers of ABA synthesis- and metabolism-related genes for qRT-PCR.
基因名称 Gene name | 基因登录号 Accession No. | 引物序列 Primer sequence | 产物大小 Amplification length/bp |
---|---|---|---|
OsUBQ5 | AK061988 | F:5’-ACCACTTCGACCGCCACTACT-3’ | 69 |
R:5’-ACGCCTAAGCCTGCTGGTT-3’ | |||
OsZEP | AB050884 | F:5’-GGATGCCATTGAGTTTGGTT-3’ | 133 |
R:5’-TGGCTGACTGAAGTCTCTCG-3’ | |||
OsNCED1 | AY838897 | F:5’-CTCACCATGAAGTCCATGAGGCTT-3’ | 126 |
R:5’-GTTCTCGTAGTCTTGGTCTTGGCT-3’ | |||
OsNCED2 | AY838898 | F:5’-GGTATGGAAACGAGGATAGTGGTT-3’ | 100 |
R:5’-TGCTTATTGTTGTGCGAGAAGTTC-3’ | |||
OsNCED3 | AY838899 | F:5’-CCCCTCCCAAACCATCCAAACCGA-3’ | 132 |
R:5’-TGTGAGCATATCCTGGCGTCGTGA-3’ | |||
OsNCED4 | AY838900 | F:5’-TCGCGCTCAGCTACAATGTC-3’ | 88 |
R:5’-ATGTCGACGTCTCGGGACTT-3’ | |||
OsNCED5 | AY838901 | F:5’-ACATCCGAGCTCCTCGTCGTGAA-3’ | 184 |
R:5’-TTGGAAGGTGTTTTGGAATGAACCA-3’ | |||
OsABA8ox1 | NM_001054390 | F:5’-CCAAGAACCCCAACGTGTTC-3’ | 101 |
R:5’-CGGGCTGGACACCATCA-3’ | |||
OsABA8ox2 | NM_001068556 | F:5’-GCGAGACGCTCCAGCTCTA-3’ | 100 |
R:5’-GGGCACCCCAGCAGATTC-3’ | |||
OsABA8ox3 | NM_001069901 | F:5’-TCCAGCGACGAGGTTGAGTA-3’ | 100 |
R:5’-AACCATCTGTTTCCACACTGACA-3’ |
Fig. 1. RBSDV infection resulted in an increase of ABA in rice. (A, Phenotype of RBSDV-infected rice plant; B, RT-PCR analysis of the RBSDV infection in mock-inoculated rice (lanes 1-3) and in RBSDV-inoculated rice (lanes 4-8); C, Content of endogenous ABA in RBSDV- and mock-inoculated rice. )
Fig. 2. Specific verification of primers used for qRT-PCR. (A, Dissociation curves of amplified products; B, The PCR products amplified by the specific primers. )
基因名称 Gene name | 侵染后天数Days after infection/d | |||
---|---|---|---|---|
8 | 12 | 16 | 60 | |
OsZEP | 4.28±0.43 | 3.03±0.39 | 0.89±0.18 | 0.40±0.06 |
OsNCED1 | 2.00±0.11 | 2.78±0.30 | 2.72±0.58 | 0.23±0.06 |
OsNCED2 | 1.55±0.17 | 0.93±0.08 | 1.43±0.32 | 0.43±0.06 |
OsNCED3 | 1.22±0.34 | 3.03±0.31 | 1.07±0.32 | 3.97±0.81 |
OsNCED4 | 1.63±0.29 | 2.58±0.34 | 3.13±0.65 | 7.66±1.46 |
OsNCED5 | 1.66±0.30 | 1.67±0.28 | 2.44±0.72 | 2.99±0.44 |
OsABA8ox1 | 3.37±0.52 | 2.37±0.21 | 0.91±0.22 | 0.57±0.23 |
OsABA8ox2 | 3.30±0.44 | 2.62±0.48 | 1.30±0.26 | 0.60±0.09 |
OsABA8ox3 | 2.36±0.27 | 1.61±0.23 | 0.54±0.11 | 1.11±0.31 |
Table 2 Relative expression levels of ABA metabolism-related genes in RBSDV-infected rice.
基因名称 Gene name | 侵染后天数Days after infection/d | |||
---|---|---|---|---|
8 | 12 | 16 | 60 | |
OsZEP | 4.28±0.43 | 3.03±0.39 | 0.89±0.18 | 0.40±0.06 |
OsNCED1 | 2.00±0.11 | 2.78±0.30 | 2.72±0.58 | 0.23±0.06 |
OsNCED2 | 1.55±0.17 | 0.93±0.08 | 1.43±0.32 | 0.43±0.06 |
OsNCED3 | 1.22±0.34 | 3.03±0.31 | 1.07±0.32 | 3.97±0.81 |
OsNCED4 | 1.63±0.29 | 2.58±0.34 | 3.13±0.65 | 7.66±1.46 |
OsNCED5 | 1.66±0.30 | 1.67±0.28 | 2.44±0.72 | 2.99±0.44 |
OsABA8ox1 | 3.37±0.52 | 2.37±0.21 | 0.91±0.22 | 0.57±0.23 |
OsABA8ox2 | 3.30±0.44 | 2.62±0.48 | 1.30±0.26 | 0.60±0.09 |
OsABA8ox3 | 2.36±0.27 | 1.61±0.23 | 0.54±0.11 | 1.11±0.31 |
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