Characteristic Analysis on a Novel Gene Interacted with OsHSP90 from Rice

doi:10.3969/j.issn.1001-7216.2009.04.02

Abstract

Abstract: OsHSP90 is an abiotic stressinducible gene in rice. From the chilling and droughtinducible cDNA library of rice, a novel gene, OsHSPBP, which interacted with OsHSP90 was identified by using yeast twohybrid system. The 1321 bp full length cDNA of OsHSPBP was cloned. Its open reading frame (ORF) predicts a 244amino acid(aa) polypeptide with calculated molecular weight of 25.5 kD. A tify motif and a CCT_2 motif were found at the aa 97-132 and aa 185-211, respectively. Sequence similarity analysis indicated that the OsHSPBP had 30.66%-70.40% identities with the tify family proteins from other plants. Among the putative promoter region, six factors matched to ciselements related to the abiotic stress response were identified using the PlantCARE software. Semiquantitative RTPCR showed that the OsHSP90 and OsHSPBP might be detected in the similar degree under different stresstreatments, suggesting that OsHSP90 and OsHSPBP could be involved in the signal transmission and response to the abiotic stress in rice.

Key words: rice, abiotic stress, inducible gene, gene expression, amino acid sequence, yeast twohybrid system

摘要： OsHSP90是水稻中一类可以在不同逆境条件下被诱导的基因。利用酵母双杂交系统，在水稻低温和干旱cDNA文库中筛选出1个与OsHSP90互作的未知基因，命名为OsHSPBP。序列分析表明，该基因全长1321 bp，编码一个具有244个氨基酸残基的多肽，推测分子量为25.5 kD。在OsHSPBP蛋白的97-132位氨基酸残基间存在tify功能域，185-211位氨基酸残基构成CCT_2基序，与其他植物中的同源蛋白相似性介于30.66%～70.40%。经分析，该基因启动子区域存在6种不同的逆境反应顺式作用元件。RTPCR半定量结果表明， OsHSP90与OsHSPBP在逆境胁迫下，均能被不同程度地诱导并呈现相似的表达趋势，说明两者可能与水稻逆境胁迫的应答和信号传递有关。

关键词: 水稻, 逆境胁迫, 诱导基因, 基因表达, 氨基酸序列, 酵母双杂交系统

MA Bingtian,﹟,WAN Jia,﹟,ZHANG Qi,﹟,JIANG Bin,XU Yongju,LI Dongmei,XU Zhengjun. Characteristic Analysis on a Novel Gene Interacted with OsHSP90 from Rice [J]. Chinese Journal of Rice Science, DOI: 10.3969/j.issn.1001-7216.2009.04.02 .

马炳田,万佳,张琦,江斌,徐永菊,李冬梅,徐正君. 水稻中一个与OsHSP90互作的新基因特性分析[J]. 中国水稻科学, DOI: 10.3969/j.issn.1001-7216.2009.04.02 .

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