中国水稻科学
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  2011, Vol. 25 Issue (3): 331-334     DOI: 10.3969/j.issn.1001-7216.2011.03.016
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Breeding of Rice Lines with Bacterial Blight Resistance Gene Xa23 by Using MarkerAssisted Selection
FAN Hong-huan1,2; WANG Lin-you2;ZHANG Li-xia2; YU Xin2; WANG Xi2;JIN Qing-sheng2; WANG Jian-jun1,2,*
1 College of Chemistry and Life Sciences, Zhejiang Normal University, Hangzhou 321004, China; 2 The Institute of Crops and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; * Corresponding author, E-mail: zwsjsxz @zaas.org
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Abstract The rice bacterial blight resistance gene Xa23, which is carried by the donor parent 18113, was introduced into the intersubspecific restorer line H705 and good grain quality restorer line H706 by crossing and molecular markerassisted selection(MAS). Seventyone and 52 homozygous lines carrying Xa23 were obtained from the 18113/H705 F3 and 18113/H706 F3 lines, respectively by PCR with EST marker C189. The bacterial blight resistance of these lines was investigated with the pathotype Ⅳ representative strain Zhe 173 by clipping method. Sixtyone and 44 lines showed the bacterial blight resistance, with the MAS accuracies of 85.92% and 84.61%, respectively. The C189 was one of the effective MAS markers for testing Xa23 gene. In addition, further research is needed to elucidate the mechanisms of higher false positive rate than theoretical exchange rate.
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Articles by authors
FAN Hong-huan
WANG Lin-you
ZHANG Li-xia
YU Xin
WANG Xi
JIN Qing-sheng
WANG Jian-jun
Key wordsrice   bacterial blight   markerassisted selection   resistance gene   breeding     
Received: 1900-01-01;
Cite this article:   
FAN Hong-huan,WANG Lin-you,ZHANG Li-xia et al. Breeding of Rice Lines with Bacterial Blight Resistance Gene Xa23 by Using MarkerAssisted Selection[J]. , 2011, 25(3): 331-334 .
 
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Resistance Performance of Rice Varieties (Combinations) to Brown Planthopper,Nilaparvata lugens, in Paddy Field

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