Genetic Analysis and Molecular Mapping of a Rice Rumpled and Twisted Leaf Mutant (rtl1)

doi:10.3969/j.issn.1001-7216.2011.03.006

Abstract

Abstract: A rumpled and twisted leaf 1 (rtl1) mutant was derived from japonica cultivar Nipponbare by ethyl methane sulfonate (EMS) treatment, which was characterized by rumpled and twisted leaf at the seedling stage. The F2 population was constructed by crossing with indica cultivars TN1 and Zhefu 802, respectively. Genetic analysis confirmed that it was controlled by one recessive nuclear gene. The closely linked SSR marker RM1155 was obtained through bulked segregant analysis．Subsequently, new STS markers were developed using published rice genome sequence, and the gene was finally located between the STS marker T1591 and SSR marker RM1359 with the distances of 0.48 and 0.96 cM, respectively. This will contribute to cloning of the target gene in further studies.

Key words: rice, leaf type, genetic analysis, gene mapping, molecular marker

摘要： 水稻扭曲叶突变体rtl1是利用甲基磺酸乙酯（EMS）诱变粳稻品种日本晴获得的。在苗期，该突变体的叶片就表现出皱缩和扭曲状。将该突变体分别与籼稻品种台中本地1号和浙辐802进行配组。遗传分析表明该突变体性状受1对隐性单基因控制。通过混合分离法，找到了位于第4染色体上的紧密连锁SSR标记RM1155，通过新发展的多态性STS标记，最终将该基因定位在STS标记T1591和SSR标记RM1359之间，其遗传距离分别为0.48和0.96 cM。为进一步克隆该基因打下了基础。

关键词: 水稻, 叶型, 遗传分析, 基因定位, 分子标记

FANG Yun-xia,SONG Xiu-juan,PENG You-lin,DONG Guo-jun,GUO Long-biao,ZENG Da-li,ZHANG Guang-heng,YAN Hong-lan,QIAN Qian. Genetic Analysis and Molecular Mapping of a Rice Rumpled and Twisted Leaf Mutant (rtl1)[J]. Chinese Journal of Rice Science, DOI: 10.3969/j.issn.1001-7216.2011.03.006 .

方云霞,宋修娟,彭友林,董国军,郭龙彪,曾大力,张光恒,颜红岚,钱前,. 水稻皱曲叶突变体rtl1的遗传分析与分子定位[J]. 中国水稻科学, DOI: 10.3969/j.issn.1001-7216.2011.03.006 .

References

[1]袁隆平. 杂交水稻超高产育种. 杂交水稻, 1997, 12(6): 1-6.

[2]朱德峰, 林贤青, 曹卫星. 不同叶片卷曲度杂交水稻的光合特性比较. 作物学报, 2001, 27(3): 329-333.

[3]朱雄涛, 汪真. 水稻高光效生理育种初探. 福建稻麦科技, 2003(6): 14-17.

[4]Scanlon M J. Developmental complexities of simple leaves. Curr Opin Plant Biol, 2000, 3(1): 31-36.

[5]Bowman J, Eshed Y, Baum S F. Establishment of polarity in angiosperm lateral organs. Trends Genet, 2002, 18(3): 134-141.

[6]Laux T, Mayer K F, Berger J, et al. The WUSCHEL gene is required for shoot and floral meristem integrity in Arabidopsis. Development, 1996, 122(1): 87-96.

[7]Schoof H, Lenhard M, Haecker A, et al. The stem cell population of Arabidopsis shoot meristems in maintained by a regulatory loop between the CLAVATA and WUSCHEL genes. Cell, 2000, 100(6): 635-644.

[8]Fletcher J C, Brand U, Running M P, et al. Signaling of cell fate decisions by CLAVATA3 in Arabidopsis shoot meristems. Science, 1999, 283(5409): 1911-1914.

[9]Lincoln C, Long J, Yamaguchi J, et al. A knotted1-like homeobox gene in Arabidopsis is expressed in the vegetative meristem and dramatically alters leaf morphology when overexpressed in transgenic plants. Plant Cell, 1994, 6(12): 1859-1876.

[10]Ito Y, Eiguchi M, Kurata N. KNOX homeobox genes are sufficient in maintaining cultured cells in an undifferentiated state in rice. Genesis, 2001, 30(4): 231-238.

[11]Sato Y, Hong S K, Tagiri A, et al. A rice homeobox gene, OSH1, is expressed before organ differentiation in a specific region during early embryogenesis. Proc Natl Acad Sci USA, 1996, 93(15): 8117-8122.

[12]Scofield S, Murray J A. KNOX gene function in plant stem cell niches. Plant Mol Biol, 2006, 60(6): 929-946.

[13]Byrne M E, Barley R, Curtis M, et al. Asymmetric leaves 1 mediates leaf patterning and stem cell function in Arabidopsis. Nature, 2000, 408(6815): 967-971.

[14]Ori N, Eshed Y, Chuck C R, et al. Mechanisms that control knox gene expression in the Arabidopsis shoot. Development, 2000, 127(24): 5523-5532.

[15]Semiarti E, Ueno Y, Tsukaya H, et al. The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem related homeobox genes in leaves. Development, 2001, 128(10): 1771-1783.

[16]Endrizzi K, Moussian B, Haecker A, et al. The SHOOT MERISTEMLESS gene is required for maintenance of undifferentiated cells in Arabidopsis shoot and floral meristems and acts at a different regulatory level than the meristem genes WUSCHEL and ZWILLE. Plant J, 1996, 10(6): 967-979.

[17]Zhong R, Ye Z H. IFL1, a gene regulating interfascicular fiber differentiation in Arabidopsis, encodes a homeodomain-leucine zipper protein. Plant Cell, 1999, 11(11): 2139-2152.

[18]Ratcliffe O J, Riechmann J L, Zhang J Z. INTERFASCICULAR FIBERLESSI is the same gene as REVOLUTA. Plant Cell, 2000, 129(3): 315-317.

[19]McConnell J R, Emery J, Eshed Y, et al. Role of PHABULOSA and PHAVOLUTA in determining radial patterning in shoots. Nature, 2001, 411(6838): 709-713.

[20]Siegfried K R, Eshed Y, Baum S F, et al. Members of the YABBY gene family specify abaxial cell fate in Arabidopsis. Development, 1999, 126(18): 4117-4128.

[21]Kumaran M K, Bowman J L, Sundaresan V. YABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis. Plant Cell, 2002, 14(11): 2761-2770.

[22]Yamaguchi T, Nagasawa N, Kawasaki S, et al. The YABBY gene DROOPING LEAF regulates carpel specification and midrib development in Oryza sativa. Plant Cell, 2004, 16(2): 500-509.

[23]Eshed Y, Baum S F, Perea J V, et al. Establishment of polarity in lateral organs of plants. Curr Biol, 2001, 11(16): 1251-1260.

[24]Emery J F, Floyd S K, Alvarez J, et al. Radial patterning of Arabidopsis shoots by class ⅢHD-ZIP and KANADⅠgenes. Curr Biol, 2003, 13(20): 1768-1774.

[25]Kerstetter R A, Bollman K, Taylor R A, et al. KANADⅠ regulates organ polarity in Arabidopsis. Nature, 2001, 411(6838): 706-709.

[26]Vogler H, Kuhlemeier C. Simple hormones but complex signaling. Curr Opin Plant Biol, 2003, 6(1): 51-56.

[27]Okadala K, Uedal J, Komaki M K, et al. Requirement of the auxin polar transport system in early stages of Arabidopsis floral bud formation. Plant Cell, 1991, 3(7): 677-684.

[28]Reinhardt D, Pesce E R, Stieger P, et al. Regulation of phyllotaxis by polar auxin transport. Nature, 2003, 426(6964): 255-260.

[29]Rhoades M W, Reinhart B J, Lim L P, et al. Prediction of plant microRNA targets. Cell, 2002, 110(4): 513-520.

[30]Juarez M T, Kui J S, Thomas J, et al. MicroRNA-mediated repression of rolled leaf1 specifies maize polarity. Nature, 2004, 428(6978): 84-88.

[31] Murray M G, Thompson W F. Rapid isolation of high molecular weight plant DNA. Nucl Acids Res, 1980, 8(19): 4321-4325.

[32]Vollbrecht E, Veit B, Sinha N, et al. The developmental gene Knotted-1 is a member of a maize homeobox gene family. Nature, 1991, 350(6315): 241-243.

[33]Smith L G, Greene B, Veit B, et al. A dominant mutation in the maize homeobox gene, Knotted-1, causes its ectopic expression in leaf cells with altered fates. Development, 1992, 116(1): 21-30.

[34]Kano-Murakami Y, Yanai T, Tagiri A, et al. A rice homeotic gene, OSH1, causes unusual phenotypes in transgenic tobacco. FEBS Lett, 1993, 334(3): 365-368.

[35]Sinha N R, Williams R E, Hake S. Overexpression of the maize homeo box gene, KNOTTED-1, causes a switch from determinate to indeterminate cell fates. Genes Dev, 1993, 7(5): 787-795.

[36]Postma-Haarsma A D, Verwoert I I, Stronk O P, et al. Characterization of the KNOX class homeobox genes Oskn2 and Oskn3 identified in a collection of cDNA libraries covering the early stages of rice embryogenesis. Plant Mol Biol, 1999, 39(2): 257-271.

[37]Hareven D, Gutfinger T, Parnis A, et al. The making of a compound leaf: Genetic manipulation of leaf architecture in tomato. Cell, 1996, 84(5): 735-744.

[38]Ma Y, Wang F, Guo J, et al. Rice OsAS2 gene, a member of LOB domain family, functions in the regulation of shoot differentiation and leaf development. J Plant Biol, 2009, 52(5): 374-381.

[39]Dai M, Hu Y, Zhao Y, et al. A WUSCHEL-LIKE HOMEOBOX gene represses a YABBY gene expression required for rice leaf development. Plant Physiol, 2007, 144(1): 380-390.

[40]Hu J, Zhu L, Zeng D, et al. Identification and characterization of NARROW AND ROLLED LEAF 1, a novel gene regulating leaf morphology and plant architecture in rice. Plant Mol Biol, 2010, 73(3): 283-292.

[41]Fujino K, Matsuda Y, Ozawa K, et al. NARROW LEAF 7 controls leaf shape mediated by auxin in rice. Mol Genet Genomics, 2008, 279(5): 499-507.

[42]Qi J, Qian Q, Bu Q, et al. Mutation of the rice Narrow leaf1 gene, which encodes a novel protein, affects vein patterning and polar auxin transport. Plant Physiol, 2008, 147(4): 1947-1459.

[43]Ueguchi-Tanaka M, Ashikari M, Nakajima M, et al. GIBBERELLIN INSENSITIVE DWARF1 encodes a soluble receptor for gibberellin. Nature, 2005, 437(7059): 693-698.

[44]Zhang G H, Xu Q, Zhu X D, et al. SHALLOT-LIKE1 is a KANADⅠ transcription factor that modulates rice leaf rolling by regulating leaf abaxial cell development. Plant Cell, 2009, 21(3): 719-735.

[45]Zhao S Q, Hu J, Guo L B, et al. Rice leaf inclination2, a VIN3-like protein, regulates leaf angle through modulating cell division of the collar. Cell Res, 2010, 20(8): 935-947.

[46]Yamamuro C, Ihara Y, Wu X, et al. Loss of function of a rice brassinosteroid insensitive1 homolog prevents internode elongation and bending of the lamina joint. Plant Cell, 2000, 12(9): 1591-1606.

[47]Tanabe S, Ashikari M, Fujioka S, et al. A novel cytochrome P450 is implicated in brassinosteroid biosynthesis via the characterization of a rice dwarf mutant, dwarf11, with reduced seed length. Plant Cell, 2005, 17(3): 776-790.

[48] Hong Z, Ueguchi-Tanaka M, Umemura K, et al. A rice brassinosteroid-deficient mutant, ebisu dwarf (d2), is caused by a loss of function of a new member of cytochrome P450. Plant Cell, 2003, 15(12): 2900-2910.

[49]Nagasawa N, Miyoshi M, Sano Y, et al. SUPERWOMAN1 and DROOPING LEAF genes control floral organ identity in rice. Development, 2003, 130(4): 705-718.

[50] 汪得凯, 张红心, 胡国成, 等. 一个水稻大叶角度突变体lla的遗传分析及基因克隆. 科学通报, 2005, 50(4): 399-401.

[51]Li W, Wu J, Weng S, et al. Characterization and fine mapping of the glabrous leaf and hull mutants (gl1) in rice (Oryza sativa L. ). Plant Cell Rep, 2010, 29(6): 617-627.

[52]Kurata N, Miyoshi K, Nonomura K, et al. Rice mutants and genes related to organ development, morphogenesis and physiological traits. Plant Cell Physiol, 2005, 46(1): 48-62.

[53]Lee J, Park J J, Kim S L, et al. Mutations in the rice liguleless gene result in a complete loss of the auricle, ligule and laminar joint. Plant Mol Biol, 2007, 65(4): 487-499.

[54]Nagasaki H, Itoh J, Hayashi K, et al. The small interfering RNA production pathway is required for shoot meristem initiation in rice. Proc Natl Acad Sci USA, 2007, 104(37): 14867-14871.