SSR-Based Analysis on Genetic Diversity of Rice Landraces from Guizhou Province, China

doi:10.3969/j.issn.1001-7216.2010.03.005

Abstract

Abstract: The genetic diversity of 74 rice landraces from Guizhou Province, China was analyzed with 21 SSR markers and six standard varieties representing typical indica and japonica ecotypes. Ninetytwo alleles were detected. The average number of alleles per locus was 4.381 with a range from 2 to 9. The Shannon′s information indices varied widely from 02793 to 1.8732 with an average value of 0.935. The expected heterozygosities ranged from 0.0988 to 0.8313 with an average value of 0.5145. Cluster analysis results showed that the similarity coefficients varied from 0.64 and 0.98 and UPGMA cluster analysis showed that the rice materials tested could be divided into four groups at a genetic similarity coefficient of 0.682. Most of the materials were scattered around typical japonica ecotypes and the genetic relationship among these landraces was not in accord with their geographical origins. The results of principal coordinates analysis and UPGMA cluster analysis revealed that the genetic structure was multiplex in the rice landraces. The rice landraces from Guizhou Province showed lower genetic diversity and most were japonica ecotypes according to SSR analysis.

Key words: rice, landrace, simple sequence repeats, genetic diversity, principal coordinates analysis

摘要： 利用21对SSR引物对74份贵州“禾”水稻品种及6份国际上常用的典型籼稻和粳稻品种进行遗传多样性研究。共检测到92个等位基因，每个位点的等位基因数变幅为2～9个，平均4.381个；平均Shannon信息指数为0.935，变幅为02793～1.8732；期望杂合度为0.5145，范围在0.0988～0.8313；品种间遗传相似系数为0.64～0.98。UPGMA聚类分析表明，在相似系数为0.682处可将“禾”品种分为4大类，大部分材料被聚类到典型粳稻的附近，品种间的亲缘关系与地理来源关系不大；主坐标分析结果与UPGMA聚类结果基本吻合。SSR检测结果表明，贵州省水稻地方品种——“禾”的多样性程度较低，且大多数属于粳稻。

关键词: 水稻, 地方品种, 微卫星标记, 遗传多样性, 主坐标分析

MA Lin,YU Xian-quan*,ZHAO Fu-sheng. SSR-Based Analysis on Genetic Diversity of Rice Landraces from Guizhou Province, China [J]. Chinese Journal of Rice Science, DOI: 10.3969/j.issn.1001-7216.2010.03.005 .

马琳,余显权,赵福胜. 贵州地方水稻品种的SSR遗传多样性分析[J]. 中国水稻科学, DOI: 10.3969/j.issn.1001-7216.2010.03.005 .

References

[1]杨黎, 周定生, 郑桂云, 等. 黔东南原生态农耕文化——禾. 贵州农业科学, 2008, 36(4): 23-26.

[2]张再兴. 贵州地方稻种资源分布及其与生态环境的关系. 贵州农业科学, 1983(3): 1-4.

[3]卢培凡. 贵州的水稻//熊振民，蔡洪法. 中国水稻. 北京: 中国农业科技出版社, 1992.

[4]杨昌润. 美丽富饶的黔东南. 贵州文史丛刊, 1994(1): 42-43.

[5]程良炳. 地方稻种——禾的特点及其利用意见. 贵州农业科学, 1981(2): 23-27.

[6]张再兴. 贵州稻种资源//应存山. 中国稻种资源. 北京: 中国农业科技出版社, 1993: 201-222.

[7]阮仁超, 杨宗烺, 陈龙, 等. 糯稻良种农虎禾在贵州立体农业生态条件下适应性初探. 贵州农业科学, 1992(5): 25-29.

[8]贾瑞海. 黔东南地方糯稻的品质研究. 贵州农业科学, 1988(3): 17-21.

[9]倪克鱼, 孙灿慧. 中国稀有稻类贵州禾的广亲和性及评价. 贵州农业科学, 2000, 28(3): 3-5.

[10]魏兴华, 袁筱萍, 余汉勇, 等. 我国常规稻主栽品种的遗传变异分析. 中国水稻科学, 2009, 23(3): 237-244.

[11]Yao Q L, Yang K C, Pan G T, et al. Genetic diversity of maize (Zea mays L.) landraces from Southwest China based on SSR data. J Genet Gen, 2007, 34(9): 851-860.

[12]Zhang L Q, Sun G L, Yan Z H, et al. Comparison of newly synthetic hexaploid wheat with its donors on SSR products. J Genet Gen, 2007, 34(10): 939-946.

[13]徐秋华, 张献龙, 聂以春, 等. 我国棉花抗枯萎病品种的遗传多样性分析. 中国农业科学, 2002, 35(3): 272-276.

[14]Dellaporta S L, Wood J, Hicks J B. A plant DNA mini preparation: Version Ⅱ. Plant Mol Biol Rep, 1983, 1: 19-21.

[15]Doyle J J, Doyle J L.Isolation of plant DNA from fresh tissue. Focus, 1990, 12: 149-151.

[16]宗绪晓, Vaughan D, Kaga A, 等. AFLP分析小豆种(Vigna angularis)内遗传多样性. 作物学报, 2003, 29(4): 562-568.

[17]Yeh F C, Yang R C, Boyle T. Popgene Version 1.31 Quick User Guide. Edmonton: Centre for International Forestry Research, University of Alberta, 1999.

[18]Kimnra M, Crow J F. The number of alleles that can bemaintainedin a finite population. Genetics, 1964, 49: 725-738.

[19]Lewontin R C. The apportionment of human diversity//Dobzhansky T, Hecht M K, Steere W C. Evolutional Biology: Vol. 6. New York: Columbia-Century-Croft, 1972: 381-398.

[20]Rohlf F J. NTSYS-pc: Numerical taxonomy and multivariate analysis system, version 2.1. New York: Exeter Publications, 2000.

[21]张晓丽, 郭辉, 王海岗, 等. 中国普通野生稻与栽培稻种SSR多样性的比较分析. 作物学报, 2008, 34(4): 591-597.

[22]黔东南州黎、从、榕三县禾考察队. 黎平从江榕江“禾”考察报告. 贵州农业科学, 1981(5): 64-70.

[23]戴继跃, 张再兴, 曾令祥, 等. 贵州地方稻种——芽期耐寒性与其原产地相关因素的探讨.种子, 1990(2): 38-41.

[24]何华勤, 贾小丽, 梁义元, 等. RAPD和ISSR标记对水稻化感种质资源遗传多态性的分析. 遗传学报, 2004, 31(9): 888-894.