中国水稻科学
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  2010, Vol. 24 Issue (3): 244-250     DOI: 10.3969/j.issn.1001-7216.2010.03.006
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Development of STS Marker KC1 for Brown Planthopper Resistance Gene Bph18(t) and Marker Assisted Selection Efficiency
LIANG Yun-tao1,2,4,#, WANG Chun-lian1,#, LAI Feng-xiang3, LIU Pi-qing2, WANG Jian1, FU Qiang3, ZHAO Kai-jun1,*
1Key Laboratory of Crop Genetics and Breeding, Ministry of Agriculture/The National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2Agricultural College, Guangxi University, Nanning 530005, China; 3 China National Rice Research Institute, Hangzhou 310006, China;4 Rice Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China; # These authors contributed equally to this paper; *Corresponding author, E-mail: zhaokj@mail.caas.net.cn
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Abstract To facilitate application of the elite brown planthopper(BPH) resistance gene Bph18(t) to rice breeding program by molecular assisted selection(MAS), a STS marker, KC1, for Bph18(t) gene was developed. To test the MAS efficiency of KC1 for Bph18(t), the F2 plants derived from the cross between Yangdao 6(i.e.9311)\[without Bph18(t), susceptible to BPH\] and C4064 \[harboring Bph18(t)\] were subjected to both bioassay of BPH resistance in the field and molecular identification with the STS marker KC1 in the laboratory. Among the F2 plants, 292 plants were resistant to BPH while the other 84 plants were susceptible, indicating a single gene \[Bph18(t)\] controlling the BPH resistance in the F2 population. The results of molecular identification showed that the MAS efficiency of KC1 for Bph18(t) was 82.6%, indicating that the STS marker KC1 could be useful to trail Bph18(t) gene in rice breeding program.
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LIANG Yun-tao
#
WANG Chun-lian
#
LAI Feng-xiang
LIU Pi-qing
WANG Jian
Key wordsresistance gene   molecular marker   rice brown planthopper   marker assisted selection     
Received: 1900-01-01;
Cite this article:   
LIANG Yun-tao,#,WANG Chun-lian et al. Development of STS Marker KC1 for Brown Planthopper Resistance Gene Bph18(t) and Marker Assisted Selection Efficiency[J]. , 2010, 24(3): 244-250 .
 
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