Chinese Journal OF Rice Science ›› 2020, Vol. 34 ›› Issue (5): 413-424.DOI: 10.16819/j.1001-7216.2020.0208
• Research Papers • Previous Articles Next Articles
Xiaoqiu WANG1, Haibo DU1, Xijun CHEN2, Mingyou LI1, Jianan WANG1, Zhiwen XU2, Zhiming FENG1,2, Zongxiang CHEN1,2, Shimin ZUO1,2,*()
Received:
2020-02-20
Revised:
2020-03-30
Online:
2020-09-10
Published:
2020-09-10
Contact:
Shimin ZUO
王小秋1, 杜海波1, 陈夕军2, 李明友1, 王嘉楠1, 许志文2, 冯志明1,2, 陈宗祥1,2, 左示敏1,2,*()
通讯作者:
左示敏
基金资助:
CLC Number:
Xiaoqiu WANG, Haibo DU, Xijun CHEN, Mingyou LI, Jianan WANG, Zhiwen XU, Zhiming FENG, Zongxiang CHEN, Shimin ZUO. Analysis of Blast Resistance Genes and Neck Blast Resistance of japonica Rice Varieties/Lines Recently Developed in Jiangsu Province[J]. Chinese Journal OF Rice Science, 2020, 34(5): 413-424.
王小秋, 杜海波, 陈夕军, 李明友, 王嘉楠, 许志文, 冯志明, 陈宗祥, 左示敏. 江苏近年育成粳稻新品种/系的稻瘟病抗性基因及穗颈瘟抗性分析[J]. 中国水稻科学, 2020, 34(5): 413-424.
Add to citation manager EndNote|Ris|BibTeX
URL: http://www.ricesci.cn/EN/10.16819/j.1001-7216.2020.0208
基因 | 染色体 | 阳性对照 | 阴性对照 | 基因 | 染色体 | 阳性对照 | 阴性对照 |
---|---|---|---|---|---|---|---|
Gene | Chr. | Control 1 | Control 2 | Gene | Chr. | Control 1 | Control 2 |
Pit | 1 | IRBLt-K59 | 丽江新团黑谷LTH | Pia | 11 | IRBLa-A | 丽江新团黑谷LTH |
Pib | 2 | IRBLb-B | 丽江新团黑谷LTH | Pik | 11 | IRBLk-Ka | 丽江新团黑谷LTH |
Pigm | 6 | 谷梅4号Gumei 4 | 丽江新团黑谷LTH | Pikm | 11 | IRBLkm-Ts | 日本晴Nipponbare |
Pid3 | 6 | 地谷Digu | 丽江新团黑谷LTH | Pikh | 11 | IRBLkh-K3 | 丽江新团黑谷LTH |
Pi25 | 6 | 地谷Digu | 丽江新团黑谷LTH | Pi1 | 11 | IRBL1-CL | 日本晴Nipponbare |
Pid2 | 6 | 地谷Digu | 丽江新团黑谷LTH | Pb1 | 11 | 镇稻18 Zhendao 18 | 日本晴Nipponbare |
Pid2 | 6 | 地谷Digu | 丽江新团黑谷LTH | Pita | 12 | IRBLta-K1 | 丽江新团黑谷LTH |
Pi3/5/i | 9 | IRBL5-M | 丽江新团黑谷LTH |
Table 1 Information of controls used in detection of resistance genes to blast disease in present study.
基因 | 染色体 | 阳性对照 | 阴性对照 | 基因 | 染色体 | 阳性对照 | 阴性对照 |
---|---|---|---|---|---|---|---|
Gene | Chr. | Control 1 | Control 2 | Gene | Chr. | Control 1 | Control 2 |
Pit | 1 | IRBLt-K59 | 丽江新团黑谷LTH | Pia | 11 | IRBLa-A | 丽江新团黑谷LTH |
Pib | 2 | IRBLb-B | 丽江新团黑谷LTH | Pik | 11 | IRBLk-Ka | 丽江新团黑谷LTH |
Pigm | 6 | 谷梅4号Gumei 4 | 丽江新团黑谷LTH | Pikm | 11 | IRBLkm-Ts | 日本晴Nipponbare |
Pid3 | 6 | 地谷Digu | 丽江新团黑谷LTH | Pikh | 11 | IRBLkh-K3 | 丽江新团黑谷LTH |
Pi25 | 6 | 地谷Digu | 丽江新团黑谷LTH | Pi1 | 11 | IRBL1-CL | 日本晴Nipponbare |
Pid2 | 6 | 地谷Digu | 丽江新团黑谷LTH | Pb1 | 11 | 镇稻18 Zhendao 18 | 日本晴Nipponbare |
Pid2 | 6 | 地谷Digu | 丽江新团黑谷LTH | Pita | 12 | IRBLta-K1 | 丽江新团黑谷LTH |
Pi3/5/i | 9 | IRBL5-M | 丽江新团黑谷LTH |
基因 Gene | 染色体 Chr. | 标记 Marker | 引物序列(5'-3') Primer sequences(5'-3') | 片段大小 Fragment size / bp | 抗/感病功能 Function | 限制性内切酶 Enzyme | 参考文献 Reference |
---|---|---|---|---|---|---|---|
Pit | 1 | tRn1 | F: GTTGGAGCTACGGTTGTTCAG | 733 | 抗 R | [20] | |
tRn2 | F: CCAAGGGATTAGGTCCTAGTG | ||||||
tN11 | R: CAGCGATGGTATGAGCACAA | 530 | 感 S | ||||
Pib | 2 | Pibdom | F: GAACAATGCCCAAACTTGAGA | 365 | 抗 R | [21] | |
R: GGGTCCACATGTCAGTGAGC | |||||||
Lys145 | F: TCGGTGCCTCGGTAGTCAGT | 803 | 感 S | ||||
R: GGGAAGCGGATCCTAGGTCT | |||||||
Pigm | 6 | GMR-3 | F: AGTTCTACTTACGGAGGAGC | 146+98 | 抗 R | [22] | |
R: AGAATTATGATAAAGAGAAAGGAA | |||||||
Actin1-1 | F: ACAAAGTTTTCAACCGGCCTA | 146 | 感 S | ||||
R: CTGGTACCCTCATCAGGCATC | |||||||
Pid3 | 6 | Pid3-dCAPS | F: TACTACTCATGGAAGCTAGTTCTC | 751 | 抗 R | BamH I | [23] |
R: ACGTCACAAATCATTCGCTC | |||||||
Pi25 | 6 | CAP3 | F: CCTCACGTTTCTACGTCTTG | 490 | 抗 R | Nde I | [24] |
R: CACACCATTTCTGATGAACC | |||||||
Pid2 | 6 | D2 | F: TTGGCTATCATAGGCGTCC | 1057 | 抗 R | MIu I | [25] |
R: ATTTGAAGGCGTTTGCGTAGA | |||||||
Pi3/5/i | 9 | M-Pi5 | F: ATAGATCATGCGCCCTCTTG | 206 | 抗 R | [26] | |
R: ATAGATCATGCGCCCTCTTG | 307 | 感 S | |||||
Pia | 11 | Pia | F: CTTTTGAGCTTGATTGGTCTGC | 116 | 抗 R | [27] | |
R: CTATTGCACCAGAGGGACCAG | 125 | 感 S | |||||
Pik | 11 | RGA4 | F: GGAAAGCTGATATGTTGTCG | 1650 - | 抗 R | ||
R:ACTCGGAGTCGGAGAGTCAG | 感 S | ||||||
Pikh | 11 | FM143 | F: CCCAACATTGGTAGTAGTGC | 285 | 抗 R | [28] | |
R: TCCTTCATACGCAAVCCAATCT | 401 | 感 S | |||||
Pikm | 11 | DKm1 | F: TGAGCTCAAGGCAAGAGTTGAGGA | 174 | 抗 R | [29] | |
R: TGTTCCAGCAACTCGATGAG | 213 | 感 S | |||||
DKm2 | F: CAGTAGCTGTGTCTCAGAACTATG | 290 | 抗 R | ||||
R: AAGGTACCTCTTTTCGGCCAG | 332 | 感 S | |||||
Pi1 | 11 | M-Pi1 | F: GTGCTGCTGTGGCTAGTTTG | 460 - | 抗 R | [30] | |
R: AGTCCCCGCTCAATTTTTCT | 感 S | ||||||
Pb1 | 11 | Pb1 | F: ATCAACGCTACCTTCCC | 159 - | 抗 R | [31] | |
R: GTGCCATCACAATTTCTTC | 感 S | ||||||
Pita | 12 | Pita-R | F: AGCAGGTTATAAGCTAGGCC | 1042 | 抗 R | [32] | |
R: CTACCAACAAGTTCATCAAA | |||||||
Pita-S | F: AGCAGGTTATAAGCTAGCTAT | 1042 | 感 S | ||||
R: CTACCAACAAGTTCATCAAA |
Table 2 Information of molecular markers used in detection of resistant genes to blast disease in present study.
基因 Gene | 染色体 Chr. | 标记 Marker | 引物序列(5'-3') Primer sequences(5'-3') | 片段大小 Fragment size / bp | 抗/感病功能 Function | 限制性内切酶 Enzyme | 参考文献 Reference |
---|---|---|---|---|---|---|---|
Pit | 1 | tRn1 | F: GTTGGAGCTACGGTTGTTCAG | 733 | 抗 R | [20] | |
tRn2 | F: CCAAGGGATTAGGTCCTAGTG | ||||||
tN11 | R: CAGCGATGGTATGAGCACAA | 530 | 感 S | ||||
Pib | 2 | Pibdom | F: GAACAATGCCCAAACTTGAGA | 365 | 抗 R | [21] | |
R: GGGTCCACATGTCAGTGAGC | |||||||
Lys145 | F: TCGGTGCCTCGGTAGTCAGT | 803 | 感 S | ||||
R: GGGAAGCGGATCCTAGGTCT | |||||||
Pigm | 6 | GMR-3 | F: AGTTCTACTTACGGAGGAGC | 146+98 | 抗 R | [22] | |
R: AGAATTATGATAAAGAGAAAGGAA | |||||||
Actin1-1 | F: ACAAAGTTTTCAACCGGCCTA | 146 | 感 S | ||||
R: CTGGTACCCTCATCAGGCATC | |||||||
Pid3 | 6 | Pid3-dCAPS | F: TACTACTCATGGAAGCTAGTTCTC | 751 | 抗 R | BamH I | [23] |
R: ACGTCACAAATCATTCGCTC | |||||||
Pi25 | 6 | CAP3 | F: CCTCACGTTTCTACGTCTTG | 490 | 抗 R | Nde I | [24] |
R: CACACCATTTCTGATGAACC | |||||||
Pid2 | 6 | D2 | F: TTGGCTATCATAGGCGTCC | 1057 | 抗 R | MIu I | [25] |
R: ATTTGAAGGCGTTTGCGTAGA | |||||||
Pi3/5/i | 9 | M-Pi5 | F: ATAGATCATGCGCCCTCTTG | 206 | 抗 R | [26] | |
R: ATAGATCATGCGCCCTCTTG | 307 | 感 S | |||||
Pia | 11 | Pia | F: CTTTTGAGCTTGATTGGTCTGC | 116 | 抗 R | [27] | |
R: CTATTGCACCAGAGGGACCAG | 125 | 感 S | |||||
Pik | 11 | RGA4 | F: GGAAAGCTGATATGTTGTCG | 1650 - | 抗 R | ||
R:ACTCGGAGTCGGAGAGTCAG | 感 S | ||||||
Pikh | 11 | FM143 | F: CCCAACATTGGTAGTAGTGC | 285 | 抗 R | [28] | |
R: TCCTTCATACGCAAVCCAATCT | 401 | 感 S | |||||
Pikm | 11 | DKm1 | F: TGAGCTCAAGGCAAGAGTTGAGGA | 174 | 抗 R | [29] | |
R: TGTTCCAGCAACTCGATGAG | 213 | 感 S | |||||
DKm2 | F: CAGTAGCTGTGTCTCAGAACTATG | 290 | 抗 R | ||||
R: AAGGTACCTCTTTTCGGCCAG | 332 | 感 S | |||||
Pi1 | 11 | M-Pi1 | F: GTGCTGCTGTGGCTAGTTTG | 460 - | 抗 R | [30] | |
R: AGTCCCCGCTCAATTTTTCT | 感 S | ||||||
Pb1 | 11 | Pb1 | F: ATCAACGCTACCTTCCC | 159 - | 抗 R | [31] | |
R: GTGCCATCACAATTTCTTC | 感 S | ||||||
Pita | 12 | Pita-R | F: AGCAGGTTATAAGCTAGGCC | 1042 | 抗 R | [32] | |
R: CTACCAACAAGTTCATCAAA | |||||||
Pita-S | F: AGCAGGTTATAAGCTAGCTAT | 1042 | 感 S | ||||
R: CTACCAACAAGTTCATCAAA |
Fig. 1. Validation of the molecular markers in determining the corresponding resistance gene using known positive and negative varieties or lines as well as 10 japonica varieties randomly selected from Jiangsu Province. In the picture, the lanes from left to right are DNA Markers, positive control, negative control and 10 random japonica varieties from Jiangsu Province; About the DNA markers, expect Pid2 and Pia were DL2000, Pit was 20 bp DNA ladder, others was DL10000; The information of positive and negative control is listed in Table 1.
Fig. 2. Distribution frequency of resistance genes in japonica rice from Jiangsu Province(A) and the distribution of gene number carried by varieties(B).
Fig. 5. Distribution of disease grade in varieties tested. EMMLJ, Early- and medium-maturity late japonica rice; LMMJ, Late-maturity medium japonica rice; MMMJ, Medium-maturity medium japonica rice.
Fig. 7. Logistic regression statistical analysis about the contribution of each resistance genes to neck blast resistance. A and B mean single gene one by one logistic regression statistical parameter chart and multigene logistic regression statistical parameter chart, respectively; In regressive analysis, if the regression coefficient reached significant or extremely significant level, then there will be a P value around the specific gene; The dark dots mean dominance ration, the dark lines mean 95% confidence interval of each dominance ration.
Fig. 8. Contribute significantly of three genes to the resistance of neck blast resistance and the comparison of disease grade differences among them and their combinations. - means the absence of the specific resistance gene; The value above each bar means the average disease grade of each gene or gene combination; The lowercase letter means significance difference at 5% level between two disease grades.
[1] | Ziegler R S, Leong S, Teng P S.Rice Blast Disease[M]. Wallingford: CAB International, 1994: 267-292. |
[2] | 王军, 杨杰, 杨金欢, 范方军, 朱金燕, 陈志德, 仲维功. Pi-ta、Pi-b基因在江苏粳稻穗颈瘟抗性育种中的价值分析[J]. 华北农学报, 2012, 27(6): 141-145. |
Wang J, Yang J, Yang J H, Fan F J, Zhu J Y, Chen Z D, Zhong W G.Analysis on breeding value of Pi-ta, Pi-b genes in japonica rice breeding with neck resistance in Jiangsu[J]. Acta Agriculturae Boreali-Sinica, 2012, 27(6): 141-145. (in Chinese with English abstract) | |
[3] | 常彭阳, 朱建生. 关于稻穗颈瘟的早期侵染过程[J]. 江西植保, 1995, 3(18): 15-16. |
Chang P Y, Zhu J S.On the early infection process of rice neck blast[J]. Jiangxi Plant Protection, 1995, 3(18): 15-16. (in Chinese) | |
[4] | 王小军, 王丽锋. 水稻穗颈瘟发病特征、原因及其对策浅析[J]. 安徽农学通报, 2010, 16(2): 90-91. |
Wang X J, Wang L F.A brief analysis of the characteristics, causes and countermeasures of neck blast in rice[J]. Anhui Agriculture Science, 2010, 16(2): 90-91. (in Chinese) | |
[5] | 宋益民, 丛国. 9种杀菌剂防治水稻穗颈瘟田间药效比较[J]. 现代农药, 2016, 2(15): 48-50. |
Song Y M, Cong G.Field efficacy comparison of nine fungicides on rice neck blast[J]. Modern Agrochemicals, 2010, 16(2): 90-91. (in Chinese) | |
[6] | 余玲, 李爱宏, 潘存红, 周长海, 刘广青, 李育红, 肖宁, 张小祥, 黄年生, 吴云雨, 戴正元.分子标记辅助选择培育抗病优质晚粳稻品种扬粳806[J]. 江苏农业科学, 2014, 42(8): 75-77. |
Yu L, Li A H, Pan C H, Zhou C H, Liu G Q, Li Y H, Xiao N, Zhang X X, Huang N S, Wu Y Y, Dai Z Y.Molecular marker-assisted selection of a high quality late japonica rice variety Yangjing 806 for disease resistance[J]. Jiangsu Agricultural Science, 2014, 42(8): 75-77. (in Chinese with English abstract) | |
[7] | Li W T, Chen M S, Yu J J, Wang J, Chen X W.Recent advances in broad-spectrum resistance to the rice blast disease[J]. Plant Biology, 2019, 50: 114-120. |
[8] | Yadav M K, Aravindan S, Ngangkham U, Prabhukarthikeyan S R, Keerthana U, Raghu S, Pramesh D, Banerjee A, Roy S, Sanghamitra P, Adak T, Priyadarshinee P, Jena M, Kar M K, Rath P C.Candidate screening of blast resistance donors for rice breeding[J]. Journal of Geneties, 2019, 98(3): 1-13. |
[9] | Wu Y Y, Yu L, Xiao N, Dai Z Y, Li Y H, Pan C H, Zhang X X, Liu G Q, Li A H.Characterization and evaluation of rice blast resistance of Chinese indica hybrid rice parental lines[J]. Crop Journal, 2017, 5(6): 509-517. |
[10] | 杨豪. 江苏省稻瘟病菌遗传多样性及水稻抗瘟基因鉴定[D]. 南京: 南京农业大学, 2010. |
Yang H.Genetic diversity of Magnaporthe grisea from rice in Jiangsu and identification of resistance rice blast gene[D]. Nanjing: Nanjing Agricultural University, 2010. (in Chinese with English abstract) | |
[11] | 刘辉, 孟德龙, 査日扬, 徐大勇. 江苏水稻品种稻瘟病主效抗性基因鉴定及应用评价[J]. 福建农业学报, 2015, 30(5): 452-458. |
Liu H, Meng D L, Zha R Y, Xu D Y.Identification and evaluation on blast resistance of rice varieties in Jiangsu[J]. Fujian Journal of Agricultural Science, 2015, 30(5): 452-458. (in Chinese with English abstract) | |
[12] | 李刚, 袁彩勇, 曹奎荣, 孙祥良, 李军, 王健, 程保山, 罗伯祥, 徐卫军, 唐九友, 储成才. 544份水稻种质稻瘟病抗性鉴定及抗性基因的分布研究[J]. 中国农业大学学报, 2018, 23(5): 22-28. |
Li G, Yuan C Y, Cao K R, Sun X L, Li J, Wang J, Cheng B S, Luo B X, Xu W J, Tang J Y, Chu C C.Evaluation and distribution of the blast resistance genes of 544 rice materials[J]. Journal of China Agricultural University, 2018, 23(5): 22-28. (in Chinese with English abstract) | |
[13] | 朱勇良, 范方军, 谢裕林, 伍英保, 乔中英, 张建栋. 江苏省迟熟中粳新品系稻瘟病抗病基因检测与抗性评价[J]. 江苏农业科学, 2018, 46(19): 106-109. |
Zhu Y L, Fan F J, Xie Y L, Wu Y B, Qiao Z Y, Zhang J D.Detection and evaluation of resistance genes of rice blast in late ripening middle japonica strain in Jiangsu Province[J]. Jiangsu Agricultural Science, 2018, 46(19): 106-109. (in Chinese with English abstract) | |
[14] | 王军, 宫丹妮, 杨杰, 朱金燕, 范方军, 李文奇, 王芳权, 仲维功. 江苏省粳稻品种抗稻瘟病基因型与穗颈瘟抗性分析[J]. 江苏农业学报, 2016, 32(2): 250-256. |
Wang J, Gong D N, Yang J, Zhu J Y, Fan F J, Li W Q, Wang F Q, Zhong W G.Relationship between rice blast resistance genotypes and neck blast resistance of japonica rice in Jiangsu Province[J]. Jiangsu Agricultural Science, 2016, 32(2): 250-256. (in Chinese with English abstract) | |
[15] | 刘士平, 李信, 汪朝阳, 李香花, 何予卿. 基因聚合对水稻稻瘟病的抗性影响[J]. 分子植物育种, 2003, (1): 22-26. |
Liu S P, Li X, Wang C Y, Li X H, He Y Q.Gene pyramiding to increase the blast resistance in rice[J]. Molecular Plant Breeding, 2003, 1(1): 22-26. (in Chinese with English abstract) | |
[16] | 陈羽. 扬稻6号背景下不同稻瘟病广谱抗性基因聚合效应研究[D]. 扬州: 扬州大学, 2016. |
Chen Y.Polymer effect of different broad-spectrum blast resistance genes under the background of YD6[D]. Yangzhou: Yangzhou University, 2016. (in Chinese with English abstract) | |
[17] | 杨光, 雷海霞, 乔利. 稻瘟病室内产孢及人工接种鉴定[J]. 种业导刊, 2018, 12: 23-24. |
Yang G, Lei H X, Qiao L.Identification of indoor spore production and inoculation of rice blast[J]. Seed Industry Tribune, 2018, 12: 23-24. (in Chinese) | |
[18] | 中华人民共和国农业部. 水稻品种试验稻瘟病抗性鉴定与评价技术规程: NY/T2646-2014[S]. |
Ministry of Agriculture of the People’s Republic of China. Technical rules for identification and evaluation of blast resistance in rice variety test: NY/T 2646-2014[S]. (in Chinese) | |
[19] | 张莉, 纪铭阳, 胡宗玉, 陈悦, 郭晓东, 陈尚上, 李少鹏. 基于随机森林和逻辑回归分类模型的烟草精选品控指标筛选[J]. 江苏农业科学, 2020, 48(3): 214-217. |
Zhang L, Ji M Y, Hu Z Y, Chen Y, Guo X D, Chen S S, Li S P.Based on random forest and logistic regression classification model selection of tobacco leaf quality control indicators[J]. Jiangsu Agricultural Science, 2020, 48(3): 214-217. (in Chinese with English abstract) | |
[20] | Hayashi K, Yasuda N, Fujita Y, Koizumi S, Yoshida H.Identification of the blast resistance gene Pit in rice cultivars using functional markers[J]. Theoretical and Applied Genetics, 2010, 121: 1357-1367. |
[21] | 刘洋, 徐培洲, 张红宇, 徐建第, 吴发强, 吴先军. 水稻抗稻瘟病Pib 基因的分子标记辅助选择与应用[J]. 中国农业科学, 2008, 41(1): 9-14. |
Liu Y, Xu P Z, Zhang H Y, Xu J D, Wu F Q, Wu X J.Marker-assisted selection and application of blast resistant gene Pib in rice[J]. Scientia Agricultura Sinica, 2008, 41(1): 9-14. (in Chinese with English abstract) | |
[22] | 王芳权, 陈智慧, 许扬, 王军, 李文奇, 范方军, 陈丽琴, 陶亚军, 仲维功, 杨杰. 水稻广谱抗稻瘟病基因PigmR功能标记的开发及应用[J]. 中国农业科学, 2019,52(6): 955-967. |
Wang F Q, Chen Z H, Xu Y, Wang J, Li W Q, Fan F J, Chen L Q, Tao Y J, Zhong W G, Yang J.Development and application of the functional marker for the broad-spectrum blast resistance gene PigmR in rice[J]. Scientia Agricultura Sinica, 2019, 52(6): 955-967. (in Chinese with English abstract) | |
[23] | Shang J J, Tao Y, Chen X W, Zou Y, Lei C L, Wang J, Li X B, Zhao X F, Zhang M J, Lu Z K, Xu J C, Cheng Z K, Wan J M, Zhu L H.Identification of a new rice blast resistance gene, Pid3, by genome wide comparison of paired nucleotide-binding site-leucine-rich repeat genes and their pseudogene alleles between the two sequenced rice genomes[J]. Genetics, 2009, 182: 1303-1311. |
[24] | Wang H M, Chen J, Shi Y F, Pan G, Shen H C, Wu J L.Development and validation of CASP markers for marker-assisted selection of rice blast resistance gene Pi25[J]. Acta Agronomic Sinica, 2012, 38(11): 1960-1968. |
[25] | 王亚, 陈献功, 尹海庆, 王越涛, 杨瑞芳, 臧之光, 王生轩. 河南主要水稻种质资源中抗稻瘟病基因的分子检测[J]. 分子植物育种, 2018, 16(10): 3203-3212. |
Wang Y, Chen X G, Yin H Q, Wang Y T, Yang R F, Zang Z G, Wang S X.Molecular detection of rice blast resistance gene in the main rice germplasms in Henan Province[J]. Molecular Plant Breeding, 2018, 16(10): 3203-3212. (in Chinese with English abstract) | |
[26] | 高利军, 高汉亮, 颜群, 周萌, 周维永, 张晋, 邓国富. 4个抗稻瘟病基因分子标记的建立及在水稻亲本中的分布[J]. 杂交水稻, 2010, 25(S1): 294-298. |
Gao L J, Gao H L, Yan Q, Zhou M, Zhou W Y, Zhang J, Deng G F.Establishment of molecular markers of four rice blast resistance genes and their distribution in rice parents[J]. Hybrid Rice, 2010, 25(S1): 294-298. (in Chinese with English abstract) | |
[27] | 潘庆华. 稻瘟病抗性基因Pia功能特异性分子标记及其方法与应用[P]. 中国广东: CN 102703443A, 2012.10.03. |
Pan Q H.Functional specific molecular markers of rice blast resistance gene Pia and their methods and applications[P]. Guangdong, China: CN102703443A, 2012.10.03. (in Chinese) | |
[28] | 王军, 杨杰, 朱金燕, 范方军, 李文奇, 王芳权, 黄转运, 仲维功. 稻瘟病抗病基因Pi-kh功能标记的开发及江苏粳稻品种中Pi-kh的变异[J]. 中国水稻科学, 2014, 28(2): 141-147. |
Wang J, Yang J, Zhu J Y, Fan F J, Li W Q, Wang F Q, Huang Z Y, Zhong W G.Development of a functional marker for rice blast resistance gene Pi-kh and natural variation at Pi-kh locus in japonica rice in Jiangsu Province, China[J]. Chinese Journal of Rice Sciences, 2014, 28(2): 141-147. (in Chinese with English abstract) | |
[29] | 张羽, 张晓娟, 冯志峰, 王胜宝, 崔明珠. 稻瘟病抗性基因Pi-km的分子标记的选择[J]. 四川农业大学学报, 2014, 32(3): 252-259. |
Zhang Y, Zhang X J, Feng Z F, Wang S B, Cui M Z. Selection of molecular marker of resistance genes Pi-km locus of rice blast[J]. Journal of Sichuan Agricultural University, 32(3): 252-259. (in Chinese with English abstract) | |
[30] | 刘开强, 伍豪, 颜群, 王威豪, 陈小林, 周维永, 李瑞芳, 高利军, 韦善富, 邓国富. 水稻抗稻瘟病基因Pi1的特异性分子标记开发及利用[J]. 西南农业学报, 2016, 29(6): 1241-1244. |
Liu K Q, Wu H, Yan Q, Wang W H, Chen X L, Zhou W Y, Li R F, Gao L J, Wei S F, Deng G F. Development and application of specific marker of blast resistance gene Pi1 in rice[J]. Southwest China Journal of Agricultural Sciences, 29(6): 1241-1244. (in Chinese with English abstract) | |
[31] | 孙立亭, 林添资, 景德道, 余波, 钱华飞, 曾生元, 李闯, 姚维成, 杜灿灿, 胡庆峰, 周义文, 龚红兵. 江苏省多基因聚合对水稻稻瘟病抗性的效应分析及Pb1基因功能标记开发[J]. 南方农业学报, 2019, 50(5): 913-923. |
Sun L T, Lin T Z, Jing D D, Yu B, Qian H F, Zeng S Y, Li C, Yao W C, Du C C, Hu Q F, Zhou Y W, Gong H B.Effects of multiple genes polymerization on rice blast resistance in Jiangsu Province and the development of functional markers of Pb1 gene[J]. Journal of Southern Agriculture, 2019, 50(5): 913-923. (in Chinese with English abstract) | |
[32] | 王忠华, 贾育林, 吴殿星, 夏英武. 水稻抗稻瘟病基因 Pita 的分子标记辅助选择[J]. 作物学报, 2004, 30(12): 1259-1265. |
Wang Z H, Jia Y L, Wu D X, Xia Y W.Molecular markers-assisted selection of the rice blast resistance gene Pi-ta[J]. Acta Agronomic Sinica, 2004, 30(12): 1259-1265. (in Chinese with English abstract) | |
[33] | 陈涛, 张亚东, 朱镇, 赵春芳, 赵庆勇, 赵凌, 周丽慧, 姚姝, 王才林. Pi-b和Pita基因在江苏省粳稻中的分布以及与穗颈瘟抗性的关系[J]. 江苏农业学报, 2016, 32(1): 1-8. |
Chen T, Zhang Y D, Zhu Z, Zhao C F, Zhao Q Y, Zhao L, Zhou L H, Yao S, Wang C L.Distribution of Pi-b and Pi-ta genes in japonica rice of Jiangsu Province and their relationship with neck blast resistance[J]. Jiangsu Journal of Agricultural Sciences, 2016, 32(1): 1-8. (in Chinese with English abstract) | |
[34] | 张善磊, 孙旭超, 陈涛, 赵春芳, 朱镇, 赵庆勇, 周丽慧, 赵凌, 姚姝, 王才林. Pi-ta、Pi-5、Pi-km和Pi-b基因在粳稻品种(系)中的分布及对穗颈瘟的抗性[J]. 江苏农业学报, 2018, 34(5): 961-971. |
Zhang S L, Sun X C, Chen T, Zhao C F, Zhu Z, Zhao Q Y, Zhou L H, Zhao L, Yao S, Wang C L.Distribution of Pi-ta, Pi-5, Pi-km and Pi-b genes in japonica rice varieties (lines) and their relationship with neck blast resistance[J]. Jiangsu Journal of Agricultural Sciences, 2018, 34(5): 961-971. (in Chinese with English abstract) | |
[35] | 曾晓珊, 杨先锋, 赵正洪, 林菲, 王玲, 潘庆华. 稻瘟病抗病基因Pia 的抗性分析及精细定位[J]. 中国科学: 生命科学, 2011, 41(1): 70-77. |
Zeng X S, Yang X F, Zhao Z H, Lin F, Wang L, Pan Q H.Characterization and fine mapping of rice blast resistance gene Pia[J]. Science in China: Life Sciences, 2011, 41(1): 70-77. (in Chinese with English abstract) | |
[36] | Khallaf A, 唐正合, 王建新, 陈长军, 周明国. 江苏省水稻品种抗稻瘟病基因型的鉴定与分析[J]. 南京农业大学学报, 2011, 34(6): 65-70. |
Khallaf Abdullah, Tang Z H, Wang J X, Chen C J, Zhou M G.Identification and analysis of resistant genotypes of rice varieties to Magnaporthe grisea in Jiangsu Province[J]. Journal of Nanjing Agricultural University, 2011, 34(6): 65-70. (in Chinese with English abstract) | |
[37] | 张柱坚, 陈子强, 顾建强, 田大刚. 稻瘟病抗性基因Pi-d2、Pid-3和Pigm不同敲除突变体的抗性评价[J]. 福建农业学报, 2018, 33(12): 1231-1236. |
Zhang Z J, Chen Z Q, Gu J Q, Tian D G.Resistance on rice blast of knockout mutants of Pi-d2, Pi-d3 and Pigm[J]. Fujian Journal of Agricultural Science, 2018, 33(12): 65-70. (in Chinese with English abstract) | |
[38] | Wang G L, Barbara V.Durable resistance to rice blast: A resistance gene pair balances immunity against crop yield[J]. Science, 2017, 355: 906. |
[39] | 向聪, 任西明, 雷东阳, 陈英. 分子标记辅助选择改良C815S的稻瘟病抗性[J]. 湖南农业大学学报: 自然科学版, 2018, 44(1): 62-65. |
Xiang C, Ren X M, Lei D Y, Chen Y.Improvement of rice blast resistance of C815S through molecular marker-assisted selection[J]. Journal Hunan Agricultural University: Natural Sciences, 2018, 44(1): 62-65. (in Chinese with English abstract) | |
[40] | 赖怡帆, 孙君玥, 张旭辉, 梁毅, 李永聪, 廖花, 黄俊, 吴婷婷, 刘雄伦. 分子标记辅助选择Pigm基因改良湘晚籼13号的稻瘟病抗性[J]. 湖南农业大学学报: 自然科学版, 2019, 45(2): 113-117. |
Lai Y F, Sun J Y, Zhang X H, Liang Y, Li Y C, Liao H, Huang J, Wu T T, Liu X L.Improving the blast resistance of Xiangwanxian 13 by molecular marker-assisted selection of the Pigm gene[J]. Journal Agricultural University: Natural Sciences, 2019, 45(2): 113-117. (in Chinese with English abstract) | |
[41] | 曾生元, 李闯, 杜灿灿, 孙立亭, 景德道, 林添资, 余波, 钱华飞, 姚维成, 周义文, 龚红兵. Pigm特异性选择标记的开发及其在粳稻穗颈瘟抗性育种中的利用[J]. 中国水稻科学, 2018, 32(5): 453-461. |
Zeng S Y, Li C, Du C C, Sun L T, Jing D D, Lin T Z, Yu B, Qian H F, Yao W C, Zhou Y W, Gong H B.Development of specific markers for Pigm in marker-assisted breeding of panicle blast resistant japonica rice[J]. Chinese Journal of Rice Science, 2018, 32(5): 453-461. (in Chinese with English abstract) | |
[42] | 李育红, 吴云雨, 戴正元, 肖宁, 刘广青, 张小祥, 潘存红, 周长海, 王闯, 孙业霞, 朱兆兵, 刘晓斌, 李爱宏. 分子标记辅助选择培育抗稻瘟病杂交籼稻新品种扬两优316[J]. 扬州大学学报: 农业与生命科学版, 2018, 39(3): 52-57. |
Li Y H, Wu Y Y, Dai Z Y, Xiao N, Liu G Q, Zhang X X, Pan C H, Zhou C H, Wang C, Sun Y X, Zhu Z B, Liu X B, Li A H.Development of a new hybrid rice variety Yangliangyou 316 with well resistance to blast disease through molecular maker-assisted selection[J]. Journal of Yangzhou University: Agricultural and Life Science Edition, 2018, 39(3): 52-57. (in Chinese with English abstract) |
[1] | DING Zhengquan, PAN Yueyun, SHI Yang, HUANG Haixiang. Comprehensive Evaluation and Comparative Analysis of Jiahe Series Long-Grain japonica Rice with High Eating Quality Based on Gene Chip Technology [J]. Chinese Journal OF Rice Science, 2024, 38(4): 397-408. |
[2] | PEI Feng, WANG Guangda, GAO Peng, FENG Zhiming, HU Keming, CHEN Zongxiang, CHEN Hongqi, CUI Ao, ZUO Shimin. Evaluation of New japonica Rice Lines with Low Cadmium Accumulation and Good Quality Generated by Knocking Out OsNramp5 [J]. Chinese Journal OF Rice Science, 2023, 37(1): 16-28. |
[3] | Zhaomeng XU, Lihua LI, Xiaoqing GAO, Zhengjie YUAN, Xin LI, Xudan TIAN, Lanlan WANG, Shaohong QU. Comparative Transcriptome Analysis of Transgenic Rice Line Carrying the Rice Blast Resistance Gene Pi9 [J]. Chinese Journal OF Rice Science, 2020, 34(3): 245-255. |
[4] | Ni CAO, Yuan CHEN, Zhijuan JI, Yuxiang ZENG, Changdeng YANG, Yan LIANG. Recent Progress in Molecular Mechanism of Rice Blast Resistance [J]. Chinese Journal OF Rice Science, 2019, 33(6): 489-498. |
[5] | Yongsheng ZHU, Jianlin BAI, Hongguang XIE, Fangxi WU, Xi LUO, Shenfei JIANG, Wei HE, Liping CHEN, Qiuhua CAI, Huaan XIE, Jianfu ZHANG. Breeding Restore Lines of Hybrid Rice by Pyramiding Genes for Resistance to White Backed Planthoppers and Brown Planthoppers [J]. Chinese Journal OF Rice Science, 2019, 33(5): 421-428. |
[6] | Zhengyan PAN, Fulin QIU, Guilan LÜ, Xiufang MA, Wenqi SHANG, Yuanjun BAI, Zhengjin XU. Analysis of Rice Blast Resistance Genes in japonica Rice Varieties in Liaoning Province [J]. Chinese Journal OF Rice Science, 2019, 33(3): 241-248. |
[7] | Xiaoxuan ZHUO, Linlin FAN, Xingyu AN, Jingwei GUO, Rui YANG, Qianchun ZENG, Qiong LUO. Mapping of a New Rice Blast Resistance Gene in Ziyu 44, a Rice Landrace from Yunnan Province, China [J]. Chinese Journal OF Rice Science, 2019, 33(1): 12-19. |
[8] | Yufei DENG, Minghao LIU, Dan WANG, Shimin ZUO, Houxiang KANG, Guoliang WANG. Origin, Distribution and Sequence Diversity of Rice Blast Resistance Locus LABR_64 in Rice [J]. Chinese Journal OF Rice Science, 2019, 33(1): 20-27. |
[9] | Wenjuan WANG, Jiyong ZHOU, Congying WANG, Jing SU, Jinqi FENG, Bing CHEN, Aiqing FENG, Jianyuan YANG, Shen CHEN, Xiaoyuan ZHU. Distribution of Eight Rice Blast Resistance Genes in indica Hybrid Rice in China [J]. Chinese Journal OF Rice Science, 2017, 31(3): 299-306. |
[10] | Hongchun RUAN, Niuniu SHI, Yixin DU, Lin GAN, Xiujuan YANG, Yuli DAI, Furu CHEN. Analysis on Resistance of Pi Genes to Predominant Races of Mangnaporthe oryzae in Fujian Province, China [J]. Chinese Journal OF Rice Science, 2017, 31(1): 105-110. |
[11] | Li-xia HUA, Wen-juan WANG, Shen CHEN, Cong-ying WANG, Lie-xian ZENG, Jian-yuan YANG, Xiao-yuan ZHU, Jing SU. Development of Specific DNA Markers for Detecting the Rice Blast Resistance Gene Alleles Pi2/9/z-t [J]. Chinese Journal OF Rice Science, 2015, 29(4): 305-310. |
[12] | HUA Lixia1,2,#, WANG Wenjuan1,2,# , CHEN Shen1,2, WANG Congying1,2, ZENG Liexian1,YANG Jianyuan1, ZHU Xiaoyuan1, SU Jing1,2,*. Development of Specific DNA Markers for Detecting the Rice Blast Resistance Gene Alleles Pi2/9/zt [J]. Chinese Journal of Rice Science, 2015, 29(3): 305-310. |
[13] | ZHAO Guozhen1, JIANG Chunmiao2, LIU Jixin1 , CHEN Yumin1, YU Tengqiong2, CHENG Zaiquan2,* . Identification and Analysis on the Rice Blast Resistance Gene Pita in Wild Rice from Yunnan, China [J]. Chinese Journal of Rice Science, 2014, 28(6): 675-680. |
[14] | YANG Yang1, LIU Jinhua1, GAO Qikang1,2,* . Knockdown of CYP81A6 Gene Affects Expression of Other Genes in Rice [J]. Chinese Journal of Rice Science, 2013, 27(5): 447-456. |
[15] | ZHAO Guozhen1, JIA Yulin2,*, YAN Zongbu3, Christopher W DEREN3, Melissa H JIA2, DAI Luyuan1,* . An Efficient, Economic, and Rapid Rice DNA Extraction Method and Its Application [J]. Chinese Journal of Rice Science, 2012, 26(4): 495-499. |
Viewed | ||||||
Full text |
|
|||||
Abstract |
|
|||||