Chinese Journal OF Rice Science ›› 2020, Vol. 34 ›› Issue (2): 135-142.DOI: 10.16819/j.1001-7216.2020.9086
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Zhibin CAO1, Yao LI2, Bohong ZENG1, Linghua MAO1, Yaohui CAI1, Xiaofeng WU1,*, Linfeng YUAN1,*
Received:
2019-07-26
Revised:
2019-10-17
Online:
2020-03-10
Published:
2020-03-10
Contact:
Xiaofeng WU, Linfeng YUAN
About author:
#These authors contributed equally to this work
曹志斌1, 李瑶2, 曾博虹1, 毛凌华1, 蔡耀辉1, 吴晓峰1,*, 袁林峰1,*
通讯作者:
吴晓峰,袁林峰
作者简介:
#共同第一作者
基金资助:
CLC Number:
Zhibin CAO, Yao LI, Bohong ZENG, Linghua MAO, Yaohui CAI, Xiaofeng WU, Linfeng YUAN. QTL Mapping for Heat Tolerance of Chalky Grain Rate of Oryza glaberrima Steud.[J]. Chinese Journal OF Rice Science, 2020, 34(2): 135-142.
曹志斌, 李瑶, 曾博虹, 毛凌华, 蔡耀辉, 吴晓峰, 袁林峰. 非洲栽培稻垩白粒率耐热性QTL的定位[J]. 中国水稻科学, 2020, 34(2): 135-142.
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URL: http://www.ricesci.cn/EN/10.16819/j.1001-7216.2020.9086
Fig. 1. Comparison of heat tolerance of chalky grain rate of CSIL05-23 and R9311 in field and artificial climatic chambers. **Significant differences at 0.01 level, t-test.
世代 Generation | 供体亲本 Donor parent (IRGC102309) | 受体亲本 Recipient parent (R9311) | 群体Population | ||||
---|---|---|---|---|---|---|---|
平均 Mean | 范围 Range | 标准差 SD | 峰度 Kurtosis | 偏度 Skewness | |||
BC6F2(n=200) | 1.074 | 2.36 | 2.18 | 1.52-2.62 | 0.23 | 0.17 | 0.12 |
BC6F3(n=368) | 1.054 | 2.23 | 2.11 | 1.48-2.68 | 0.31 | 0.23 | 0.32 |
BC6F4(n=430) | 1.082 | 2.06 | 2.03 | 1.42-2.56 | 0.27 | 0.17 | 0.12 |
Table 1 Phenotypic variation of insensitive value of heat tolerance of chalky grain rate in BC6F2, BC6F3 and BC6F4 population and their parents.
世代 Generation | 供体亲本 Donor parent (IRGC102309) | 受体亲本 Recipient parent (R9311) | 群体Population | ||||
---|---|---|---|---|---|---|---|
平均 Mean | 范围 Range | 标准差 SD | 峰度 Kurtosis | 偏度 Skewness | |||
BC6F2(n=200) | 1.074 | 2.36 | 2.18 | 1.52-2.62 | 0.23 | 0.17 | 0.12 |
BC6F3(n=368) | 1.054 | 2.23 | 2.11 | 1.48-2.68 | 0.31 | 0.23 | 0.32 |
BC6F4(n=430) | 1.082 | 2.06 | 2.03 | 1.42-2.56 | 0.27 | 0.17 | 0.12 |
Fig. 2. Genetic linkage map and likelihood intervals for QTL associated with heat tolerance of chalky grain rate. The left and right bars and whiskers indicate 1 logarithm of the odds (LOD) and 2 LOD likelihood intervals from BC6F3 and BC6F4 populations, respectively.
性状 Character | 群体 Population | 区间 Interval | LOD | 表型方差 Phenotypic variance/% | 加性效应 Additive effect/% |
---|---|---|---|---|---|
垩白粒率高温钝感值IV | BC6F3 | RM1200-RM5796 | 6.3 | 11.3 | -5.9 |
BC6F4 | RM1200-RM5796 | 7.4 | 17.5 | -11.8 | |
高温胁迫下的垩白粒率X1 | BC6F3 | RM1200-RM5796 | 5.9 | 9.5 | -5.6 |
BC6F4 | RM1200-RM5796 | 6.9 | 16.7 | -12.5 | |
正常温度条件下的垩白粒率X2 | BC6F3 | RM1200-RM5796 | 6.2 | 12.6 | -5.7 |
BC6F4 | RM1200-RM5796 | 7.1 | 16.5 | -11.2 |
Table 2 QTL analysis of heat tolerance of chalky grain rate in BC6F3 and BC6F4 populations.
性状 Character | 群体 Population | 区间 Interval | LOD | 表型方差 Phenotypic variance/% | 加性效应 Additive effect/% |
---|---|---|---|---|---|
垩白粒率高温钝感值IV | BC6F3 | RM1200-RM5796 | 6.3 | 11.3 | -5.9 |
BC6F4 | RM1200-RM5796 | 7.4 | 17.5 | -11.8 | |
高温胁迫下的垩白粒率X1 | BC6F3 | RM1200-RM5796 | 5.9 | 9.5 | -5.6 |
BC6F4 | RM1200-RM5796 | 6.9 | 16.7 | -12.5 | |
正常温度条件下的垩白粒率X2 | BC6F3 | RM1200-RM5796 | 6.2 | 12.6 | -5.7 |
BC6F4 | RM1200-RM5796 | 7.1 | 16.5 | -11.2 |
Fig. 3. Mapping of qHTCGR5 by a substitution mapping strategy. Linkage map of the QTLs region produced with 380 BC6F3 plants. The number of recombinants between adjacent markers is indicated under the linkage map. Progeny testing of BC6F4 homozygous recombinants delimited the qHTCGR5 locus to the region between markers RM1200 and RM5796. The 90 recombinants were grouped into 14 groups based on genotypes. The numbers of recombinants in each group and phenotypic difference of each group from the controls CSIL05-23 and R9311 for mean insensitive value of heat tolerance of chalky grain rate are shown on the right. An “a” following the phenotypic value indicates that the mean phenotypic value of recombinant was not significantly different from that of R9311 at P < 0.05;a “b”indicates that the mean phenotypic value of recombinant was not significantly different from that of CSIL05-23 at P < 0.05.
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