Chinese Journal OF Rice Science ›› 2020, Vol. 34 ›› Issue (2): 143-149.DOI: 10.16819/j.1001-7216.2020.9085
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Feng MENG, Yaling ZHANG*(), Xuehui JIN
Received:
2019-07-21
Revised:
2019-11-21
Online:
2020-03-10
Published:
2020-03-10
Contact:
Yaling ZHANG
通讯作者:
张亚玲
基金资助:
CLC Number:
Feng MENG, Yaling ZHANG, Xuehui JIN. Detection and Analysis of Magnaporthe oryzae Avirulent Gene AVR-Pita and Its Homologous Genes in Heilongjiang Province[J]. Chinese Journal OF Rice Science, 2020, 34(2): 143-149.
孟峰, 张亚玲, 靳学慧. 黑龙江省稻瘟病菌无毒基因AVR-Pita及其同源基因的检测与分析[J]. 中国水稻科学, 2020, 34(2): 143-149.
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URL: http://www.ricesci.cn/EN/10.16819/j.1001-7216.2020.9085
无毒基因 Avr-gene | 前引物序列 Forward primer (5′-3′) | 后引物 Reverse primer (5′-3′) | 目的片段长度 Length of target fragment/bp |
---|---|---|---|
AVR-Pita1 | TGCCAATAGACTAGCTTCCG | ATTCCCTCCATTCCAACACT | 1957 |
AVR-Pita2 | TTTCGGCCCAACTCCGGTCC | TAAAGGGTCCACTGACCCCG | 1642 |
AVR-Pita3 | AAATATTACCTGCCAGCTGG | CTAAACGAATCGACGCTCCC | 1487 |
Table 1 Primers for Magnaporthe oryzae avirulent gene AVR-Pita amplification.
无毒基因 Avr-gene | 前引物序列 Forward primer (5′-3′) | 后引物 Reverse primer (5′-3′) | 目的片段长度 Length of target fragment/bp |
---|---|---|---|
AVR-Pita1 | TGCCAATAGACTAGCTTCCG | ATTCCCTCCATTCCAACACT | 1957 |
AVR-Pita2 | TTTCGGCCCAACTCCGGTCC | TAAAGGGTCCACTGACCCCG | 1642 |
AVR-Pita3 | AAATATTACCTGCCAGCTGG | CTAAACGAATCGACGCTCCC | 1487 |
Fig. 1. PCR detection of AVR-Pita and its homologous genes amplification to the tested strains. A, Amplification result of AVR-Pita1; B, Amplification result of AVR-Pita3; M1, Marker DL2000; +, Presence; -, Absence; 1, Low band; 2, Double bands; 3, Three bands.
地区Area | 供试菌株数 Number of strains | 无毒基因检出率 Detection rate of avirulence genes /% | ||||
---|---|---|---|---|---|---|
AVR-Pita1 | AVR-Pita2 | AVR-Pita3 | ||||
齐齐哈尔甘南 Gannan, Qiqihar | 16 | 6.2 | 0 | 81.3 | ||
大庆杜蒙Dumeng, Daqing | 19 | 0 | 0 | 73.7 | ||
大庆肇源 Zhaoyuan, Dayuan | 11 | 0 | 0 | 100.0 | ||
绥化望奎 Wangkui, Suihua | 7 | 42.9 | 0 | 28.6 | ||
绥化庆安 Anqing, Suihua | 14 | 42.9 | 0 | 71.4 | ||
哈尔滨通河 Tonghe, Harbin | 16 | 31.2 | 0 | 31.2 | ||
哈尔滨依兰Yilan, Harbin | 23 | 52.2 | 0 | 52.2 | ||
哈尔滨方正Fangzheng, Harbin | 4 | 0 | 0 | 50.0 | ||
哈尔滨尚志 Shangzhi, Harbin | 10 | 0 | 0 | 80.0 | ||
佳木斯桦川 Huachuan, Jiamusi | 10 | 90.0 | 0 | 80.0 | ||
佳木斯汤原 Tangyuan, Jiamusi | 22 | 22.7 | 0 | 50.0 | ||
佳木斯桦南 Huanan, Jiamusi | 15 | 86.7 | 0 | 53.3 | ||
鹤岗绥滨 Suibin, Hegang | 16 | 68.7 | 0 | 62.5 | ||
鸡西虎林 Hulin, Jixi | 9 | 33.3 | 0 | 44.4 | ||
鸡西密山 Mishan, Jixi | 10 | 50.0 | 0 | 20.0 | ||
合计 | 202 | 36.1 | 0 | 59.4 |
Table 2 Distribution of AVR-Pita1 and its homologous genes of Magnaporthe oryzae in Heilongjiang Province in 2017.
地区Area | 供试菌株数 Number of strains | 无毒基因检出率 Detection rate of avirulence genes /% | ||||
---|---|---|---|---|---|---|
AVR-Pita1 | AVR-Pita2 | AVR-Pita3 | ||||
齐齐哈尔甘南 Gannan, Qiqihar | 16 | 6.2 | 0 | 81.3 | ||
大庆杜蒙Dumeng, Daqing | 19 | 0 | 0 | 73.7 | ||
大庆肇源 Zhaoyuan, Dayuan | 11 | 0 | 0 | 100.0 | ||
绥化望奎 Wangkui, Suihua | 7 | 42.9 | 0 | 28.6 | ||
绥化庆安 Anqing, Suihua | 14 | 42.9 | 0 | 71.4 | ||
哈尔滨通河 Tonghe, Harbin | 16 | 31.2 | 0 | 31.2 | ||
哈尔滨依兰Yilan, Harbin | 23 | 52.2 | 0 | 52.2 | ||
哈尔滨方正Fangzheng, Harbin | 4 | 0 | 0 | 50.0 | ||
哈尔滨尚志 Shangzhi, Harbin | 10 | 0 | 0 | 80.0 | ||
佳木斯桦川 Huachuan, Jiamusi | 10 | 90.0 | 0 | 80.0 | ||
佳木斯汤原 Tangyuan, Jiamusi | 22 | 22.7 | 0 | 50.0 | ||
佳木斯桦南 Huanan, Jiamusi | 15 | 86.7 | 0 | 53.3 | ||
鹤岗绥滨 Suibin, Hegang | 16 | 68.7 | 0 | 62.5 | ||
鸡西虎林 Hulin, Jixi | 9 | 33.3 | 0 | 44.4 | ||
鸡西密山 Mishan, Jixi | 10 | 50.0 | 0 | 20.0 | ||
合计 | 202 | 36.1 | 0 | 59.4 |
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