Functional Analysis of Zinc Finger Protein Gene OsZFP1 in Rice

doi:10.3969/j.issn.1001-7216.2015.02.004

Abstract

Abstract:

The rice protein OsZfp1 which interacts with MoSp1 is a C3HC4-type RING-finger protein predicted by bioinformatic tools. The transcriptional changes of OsZFP1 gene during Magnaporthe oryzae infection showed that the expression level of OsZFP1 peaked with 3.8 folds at 18 hours after spore-spaying inoculation, revealing that OsZFP1might respond to M. oryzae invasion. The OsZFP1 overexpression transgenic rice plants were gained through modified Agrobacterium-mediated transformation. The statistic analysis indicated that the overexpression plants confer resistance against M. oryzae. In conclusion, the OsZFP1 gene may play an important role in rice immunity against M. oryzae.

Key words: Magnaporthe oryzae, zinc finger protein, transgenic rice

摘要：

生物信息学分析表明,稻瘟病菌丝氨酸蛋白酶MoSp1在水稻中的互作蛋白OsZfp1,为含有环指结构域的C3HC4型锌指蛋白。对稻瘟病菌侵染过程中OsZFP1基因的表达动态分析表明,OsZFP1基因表达水平在稻瘟病菌Guy11孢子悬浮液接种水稻后缓慢升高,接种后18 h达到最高峰,约为3.8倍,这表明该基因响应稻瘟病菌的侵染。利用改进的农杆菌介导的转基因技术成功获得OsZFP1基因的过表达植株。抗性分析表明OsZFP1过表达植株的整体抗稻瘟病能力得到显著提高。说明OsZFP1基因在水稻抵抗稻瘟病菌侵染过程中扮演着十分重要的角色。

关键词: 稻瘟病菌, 锌指蛋白, 转基因水稻

CLC Number:

He LI, Yi-juan HAN, Yi-juan LIN, Li-hua LIU, Cheng-kang ZHANG, Lian-hu ZHANG, Zong-hua WANG, Guo-dong LU. Functional Analysis of Zinc Finger Protein Gene OsZFP1 in Rice[J]. Chinese Journal OF Rice Science, 2015, 29(2): 135-140.

李贺, 韩艺娟, 林艺娟, 刘丽华, 张承康, 张连虎, 王宗华, 鲁国东. 水稻锌指蛋白基因OsZFP1的功能分析[J]. 中国水稻科学, 2015, 29(2): 135-140.

Figures/Tables 6

Fig. 1. Structure of T-DNA of pCXDR. LB, Left border; RB, Right border; htpⅡ, Hygromycin phosphotransferaseⅡ; CaMV35S, Cauliflower mosaic virus 35S promoter; DsRed, Discosoma red fluorescent protein; ccdB, Control of cell death; NosT, Nopaline synthase terminator.

Fig. 2. Sequence homology analysis of RING finger protein. Rectangular boxes indicate zinc finger protein domain C-X2-C-X(9-39)-C-X(1-3)-H-X(2-3)-C-X2-C-X(4-8)-C-X2-C. The black amino acids are the most conservative cysteine and valine residues, followed by the red amino acids.

Fig.3. Transcriptional level of OsZFP1 gene in the rice leaves under rice blast challenge.

Fig.4. Detection of OsZFP1 gene in recombinant plasmid.

Fig. 5. Agrobacterium mediated transformation of rice. A, Rice callus induction; B, Differentiation of resistant callus; C, Seedling rooting.

Fig. 6. Infectious degree of OsZFP1 over expressed rice.

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