Seedling Growth Characteristics of Hybrids Between Transgenic Rice with cry2A*/bar Genes and Weedy Rice

doi:10.16819/j.1001-7216.2023.220612

Abstract

Abstract:

【Objectives】 The study aims to evaluate the seedling growth characteristics of hybrids between transgenic rice T2A-1 with cry2A*/bar gene and weedy rice, and lay an experimental basis for evaluating the potential ecological risks of gene flow from T2A-1 to weedy rice. 【Methods】 F₁⁺-F₄⁺ (positive) hybrids (those with transgenic insertion using the transgenic line T2A-1 as the paternal parent), F₁⁻ -F₄⁻(negative) hybrids(those using the non-transgenic receptor Minghui 63 (MH63) as the paternal parent) with three weedy rice (WRMM, WRTZ and WRYY) accessions, were used as experimental materials. Under hydroponic culture, germinability was measured. Eight root development indexes were measured and relative root development index under normal nitrogen (40 mg/L) and low nitrogen (20 mg/L), and the seedling growth characteristics of hybrids were evaluated. 【Results】 Transgenic positive hybrids had similar 个germinability to transgenic negative hybrids, but both showed weeker germination potential and germination index compared with the corresponding weedy rice. There were no significant differences in plant height, whole plant dry biomass, and root development between T2A-1 and MH63, and between transgenic positive and transgenic negative hybrids under normal nitrogen conditions. Compared with the weedy rice, hybrids derived from WRMM displayed significantly lower plant height, but similar whole plant dry biomass and the index of root development. Hybrids of WRYY displayed similar seedling growth characteristics with WRYY. While hybrids derived from WRTZ had significantly greater plant height, but significantly lower whole plant dry biomass and index of root development. Under low nitrogen conditions, transgenic positive hybrids had similar or significantly lower plant height, whole plant dry biomass and index of root development than transgene negative hybrids. The relative root development indexes of all hybrids were significantly lower compared with their corresponding weedy rice. 【Conclution】 Compared with their corresponding weedy rice, transgenic positive hybrids displayed equivalent or lower seedling growth momentum under normal nitrogen conditions, but significantly weaker growth under low nitrogen conditions.

Key words: dual-transgenic rice, T2A-1, weedy rice, hybrids, seedling growth characteristic, root development

摘要：

【目的】 考查转cry2A*/bar基因水稻T2A-1与杂草稻的杂交后代苗期特性，为评估T2A-1抗性基因向杂草稻漂移可能引起的生态风险提供参考。【方法】 在水培条件下，以T2A-1、受体水稻明恢63(MH63)、来源于江苏泰州、广东茂名、湖南益阳的3种杂草稻（分别命名为WRTZ，WRMM，WRYY）及其杂交F₁~F₄为试验材料（以T2A-1为父本或母本的携带转基因的为抗性杂交后代，以受体水稻为父本或母本的为非抗性杂交后代），测定供试材料种子发芽力，幼苗在正常氮（40 mg/L）和低氮（20 mg/L）下的株高、整株干物质量以及根长、不定根数、平均直径等8个根系指标，计算根系总体发育指标，评价T2A-1与杂草稻抗性杂交后代的苗期生长特性。【结果】 抗性杂交后代的发芽力与非抗性杂交后代相当，但较杂草稻弱。在正常氮条件下，T2A-1与MH63以及抗性杂交后代与非抗性杂交后代的苗期生长指标相当；抗性杂交后代与相应杂草稻相比，WRMM杂交后代的株高显著低于WRMM，但整株干物质量与根系总体发育指标相当；WRYY杂交后代各苗期生长指标均与WRYY相当；WRTZ杂交后代株高显著高于WRTZ，但整株干物质量及根系指标均显著低于WRTZ。在低氮环境下，抗性杂交后代各苗期生长指标均显著低于或相当于非抗性杂交后代；抗性和非抗性杂交后代的相对根系发育指标均显著低于相应的杂草稻。【结论】 T2A-1与杂草稻的抗性杂交后代的苗期生长特性在正常氮条件下相当于或低于亲本杂草稻，但在低氮条件下，总体上均显著弱于杂草稻。

关键词: 复合性状转基因水稻, T2A-1, 杂草稻, 杂交后代, 苗期生长特性, 根系发育

XIAO Lequan, LI Lei, DAI Weimin, QIANG Sheng, SONG Xiaoling. Seedling Growth Characteristics of Hybrids Between Transgenic Rice with cry2A*/bar Genes and Weedy Rice[J]. Chinese Journal OF Rice Science, 2023, 37(4): 347-358.

肖乐铨, 李雷, 戴伟民, 强胜, 宋小玲. 转cry2A*/bar基因水稻与杂草稻杂交后代的苗期生长特性[J]. 中国水稻科学, 2023, 37(4): 347-358.

Figures/Tables 9

References 51

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试验材料编号 Accession of material		籼粳型 Subspecies	果皮颜色 Pericarp colour
WRMM	广东茂名 Maoming, Guangdong	籼型indica	红色Red
WRTZ	江苏泰州 Taizhou, Jiangsu	偏籼型indica-clinous	红色Red
WRYY	湖南益阳 Yiyang, Hunan	籼型indica	红色Red
MH63	华中农业大学 Huazhong Agricultural University	籼型indica	白色White
T2A-1	华中农业大学 Huazhong Agricultural University	籼型indica	白色White

试验材料编号 Accession of material		籼粳型 Subspecies	果皮颜色 Pericarp colour
WRMM	广东茂名 Maoming, Guangdong	籼型indica	红色Red
WRTZ	江苏泰州 Taizhou, Jiangsu	偏籼型indica-clinous	红色Red
WRYY	湖南益阳 Yiyang, Hunan	籼型indica	红色Red
MH63	华中农业大学 Huazhong Agricultural University	籼型indica	白色White
T2A-1	华中农业大学 Huazhong Agricultural University	籼型indica	白色White

材料类型 Type	杂交方式 Hybrid (♀×♂)	表示形式 Abbreviation
F⁺	WRMM×T2A-1	F_nMM⁺
	WRTZ×T2A-1	F_nTZ⁺
	WRYY×T2A-1	F_nYY⁺
F⁻	WRMM×MH63	F_nMM⁻
	WRTZ×MH63	F_nTZ⁻
	WRYY×MH63	F_nYY⁻
RF⁺	T2A-1×WRMM	RF_nMM⁺
	T2A-1×WRTZ	RF_nTZ⁺
	T2A-1×WRYY	RF_nYY⁺
RF⁻	MH63×WRMM	RF_nMM⁻
	MH63×WRTZ	RF_nTZ⁻
	MH63×WRYY	RF_nYY⁻

材料类型 Type	杂交方式 Hybrid (♀×♂)	表示形式 Abbreviation
F⁺	WRMM×T2A-1	F_nMM⁺
	WRTZ×T2A-1	F_nTZ⁺
	WRYY×T2A-1	F_nYY⁺
F⁻	WRMM×MH63	F_nMM⁻
	WRTZ×MH63	F_nTZ⁻
	WRYY×MH63	F_nYY⁻
RF⁺	T2A-1×WRMM	RF_nMM⁺
	T2A-1×WRTZ	RF_nTZ⁺
	T2A-1×WRYY	RF_nYY⁺
RF⁻	MH63×WRMM	RF_nMM⁻
	MH63×WRTZ	RF_nTZ⁻
	MH63×WRYY	RF_nYY⁻

材料 Type	发芽率 Germination percentage/%	发芽势 Germination potential/%	发芽指数 Germination index
F₁MM⁺	97.50±1.03 ab	68.50±0.83 b	24.74±0.10 b
F₁MM^―	99.25±0.65 a	68.25±1.56 b	24.67±0.31 b
RF₁MM⁺	97.00±0.94 ab	67.75±1.71 b	24.32±0.33 b
RF₁MM^―	96.00±0.61 b	70.00±1.22 b	24.76±0.19 b
WRMM	98.50±0.75 ab	80.25±0.74 a	31.36±0.60 a
F₁YY⁺	98.25±0.74 a	62.25±1.98 bc	21.03±0.64 b
F₁YY^―	97.50±0.43 a	63.25±2.30 b	20.63±0.88 b
RF₁YY⁺	98.00±0.61 a	57.50±0.25 c	20.79±0.20 b
RF₁YY^―	96.00±0.35 a	60.00±0.94 bc	21.07±0.38 b
WRYY	96.50±1.03 a	74.00±0.94 a	24.35±0.26 a
F₁TZ⁺	98.00±0.79 a	83.00±1.46 a	31.42±0.61 b
F₁TZ^―	98.00±0.79 a	83.50±2.02 a	32.31±0.60 b
RF₁TZ⁺	99.25±0.41 a	82.00±1.46 a	32.07±0.52 b
RF₁TZ^―	98.75±0.54 a	85.25±1.95 a	32.73±0.56 b
WRTZ	99.00±0.35 a	79.50±0.43 a	36.57±0.42 a