Chinese Journal OF Rice Science ›› 2015, Vol. 29 ›› Issue (1): 91-96.DOI: 10.3969/j.issn.1001-7216.2015.01.011

• Orginal Article • Previous Articles     Next Articles

Construction of a Vector for Large DNA Fragment Transformation in Magnaporthe oryzae

Ya-ping CHEN, Wen-xiao SHI, Hong-kai WANG*()   

  1. College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China
  • Received:2014-03-28 Revised:2014-06-11 Online:2015-01-10 Published:2015-01-10
  • Contact: Hong-kai WANG

稻瘟病菌大片段DNA转化载体的构建

陈亚平, 施文骁, 王洪凯*()   

  1. 浙江大学 农业与生物技术学院, 杭州 310058
  • 通讯作者: 王洪凯
  • 基金资助:
    国家自然科学基金资助项目(30670072);浙江省自然科学基金资助项目(Y305068)

Abstract:

It is an important means to study function of gene cluster using special vectors for large DNA fragment transformation. A vector was constructed for large DNA fragment transformation based on cosmid super Cos I vector and pBHt2 vector. More than 30 kb DNA fragment was transferred into the rice blast fungus successfully using Agrobacterium tumefaciens-mediated transformation. The results will promote functional analysis of gene cluster in Magnaporthe oryzae.

Key words: vector, transformation, large DNA fragment, Magnaporthe oryzae

摘要:

构建能够转化大片段DNA的载体是进行基因簇功能分析的重要手段。利用cosmid载体和pBHt2载体构建了一个可以在稻瘟病菌中转化大片段DNA的载体,通过农杆菌介导的转化方法,成功地将超过30 kb的大片段DNA转化到稻瘟病菌中。研究结果将促进稻瘟病菌基因簇的功能分析。

关键词: 载体, 转化, 大片段DNA, 稻瘟病菌

CLC Number: