Global Genome Expression Analysis of Root Genes under Drought Stress in Weedy Rice and Up-land Rice

doi:10.16819/j.1001-7216.2016.5180

Abstract

Abstract:

The expression changes of root genes in drought-resistance weedy rice HEB07-2 (Oryza sativa f. spontanea) and up-land rice IAPAR9 (Oryza sativa) were analyzed under polyethylene glycol(PEG)-simulated with drought stress condition with Affymetrix GeneChip rice genome array. The results indicated that the extent and direction of transcriptome response of HEB07-2 and IAPAR9 to drought differed greatly. For HEB07-2, among 6878 expressed genes, 4693 were up-regulated and 2185 were down-regulated under drought condition. For IAPAR9, among 2923 expressed genes,983 were up-regulated and 1940 were down-regulated. Analysis of differentially expressed genes in HEB07-2 and IAPAR9 showed that the weedy rice HEB07-2 had a higher changing fold than the up-land rice IAPAR9. Gene ontology analysis revealed that genes of HEB07-2 in potassium ion transporting(GO: 0006813), secondary metabolite(GO: 0019748), cell growth(GO: 0016049), glucose metabolism(GO:0006006), transmembrane ion transporter activity(GO:0015075), ferroheme coalition(GO:0020037), oxidordeuctase activity(GO:0016491)were significantly up-regulated in comparing with IAPAR9. These were consistent with the physiological and phenotypic data.

Key words: weedy rice, root, gene expression profiling, drought resistance

摘要：

利用Affymetrix水稻表达谱芯片(GeneChip Rice Genome Array)研究抗旱杂草稻HEB07-2与巴西陆稻(IAPAR9)在PEG模拟干旱胁迫下根系基因表达变化。结果表明,杂草稻HEB07-2转录组对干旱信号的响应程度与方向均与巴西陆稻存在很大差异,HEB07-2以正向调控为主,而巴西陆稻以负调控为主。干旱胁迫下杂草稻HEB07-2与巴西陆稻分别有6878个和2923个基因表达,其中HEB07-2受干旱胁迫诱导上调的基因有4693个,下调的基因有2185个;而巴西陆稻则分别有983个和1940个。在差异基因表达的倍数上也是HEB07-2高于巴西陆稻。进一步的GO分析表明,在干旱胁迫下,HEB07-2和IAPAR9根系基因响应途径的差异表现为HEB07-2在钾离子转运(GO:0006813)、次生物质代谢(GO:0019748)、细胞生长(GO:0016049)、葡萄糖代谢(GO:0006006)、跨膜离子转运器活性(GO:0015075)、亚铁血红素结合体(GO:0020037)、氧化还原酶活性(GO:0016491)等方面基因显著上调,这与生理数据和表型数据一致。

关键词: 杂草稻, 根系, 表达谱芯片, 耐旱

CLC Number:

Q786
Q945.78

Guo-hua DING, Jian SUN, Guang YANG, Feng-ming ZHANG, Liang-ming BAI, Shi-chen SUN, Shu-kun JIANG, Tong-tong WANG, Hong-liang ZHENG, Tian-shu XIA, Xi-hong SHEN, Dian-rong MA, Wen-fu CHEN. Global Genome Expression Analysis of Root Genes under Drought Stress in Weedy Rice and Up-land Rice[J]. Chinese Journal OF Rice Science, 2016, 30(5): 458-468.

丁国华, 孙健, 杨光, 张凤鸣, 白良明, 孙世臣, 姜树坤, 王彤彤, 郑洪亮, 夏天舒, 沈希宏, 马殿荣, 陈温福. 干旱胁迫下杂草稻和栽培稻根系基因表达差异研究[J]. 中国水稻科学, 2016, 30(5): 458-468.

Figures/Tables 10

Table 1 Primers used for real-time quantitative PCR.

基因 Gene	正向引物 Forward primer(5'- 3')
LOC_Os03g37840	CGAAATAAGAAGGACGGATGG	TGATGGGAGCAAGACAAAGGTA
LOC_Os04g52390	TCAAGAACCAAAGTCAGATAGGAA	GAAAGCACAATAAAGGTGACAACTAG
LOC_Os08g32840	CTCAACTACACCTACCGAAACGC	TCCAACTTGTGCAGGAACTCAT
LOC_Os02g02400	GAGGCAGAAGGCGACGATA	GAGGTAGTTGACCCAGATGGC
Actin	GGAACTGGTATGGTCAAGGC	AGTCTCATGGATACCCGCAG

Fig. 1. Ven figure of HEB07-2 and IAPAR9 regulated genes under drought.

Fig. 2. Gene numbers of HEB07-2 and IAPAR9 induced and repressed by drought.

Table 2 Up- and down-regulated fold of genes in HEB07-2 and IAPAR9.

材料 Material	下调表达基因 Down-regulated gene				上调表达基因 Up-regulated gene			未变个数 No change
材料 Material	下调个数 No. of down- regulated gene	最大 Max	平均 Average		上调个数 No. of up- regulated genes	最大 Max	平均 Average	未变个数 No change
样品19/样品1 Sample 19/Sample 1	2185	84.6		4.3	4693	58.4	3.4	47 947
样品20/样品2 Sample 20/Sample 2	1940	20.6		2.9	983	44.2	2.9	51 902

Fig. 3. Change folds of HEB07-2 and IAPAR9 under drought condition.

Fig. 4. Distribution of regulated genes on rice chromosome under drought stress. A, HEB07-2; B, IAPAR9.

Fig. 5. Unknow genes of HEB07-2 and IAPAR9 under drought stress.

Fig. 6. The physiological index change in HEB07-2 and IAPAR9 under drought condition.

Fig. 7. Phenotype of HEB07-2 and IAPAR9 under drought condition.

Fig. 8. Comparison of microarray data with real-time PCR results in HEB07-2 and IAPAR9. A, HEB07-2; B, IAPAR9; 1, LOC_Os03g37840; 2, LOC_Os04g52390; 3, LOC_Os08g32840; 4, LOC_Os02g02400.

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