Chinese Journal OF Rice Science ›› 2015, Vol. 29 ›› Issue (2): 200-207.DOI: 10.3969/j.issn.1001-7216.2015.02.012
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Jin-dan WU1, Qian CHEN2, Xiao-xi LIU1, Fu-cheng LIN1, Qi-kang GAO3, Bing-gan LOU1,*()
Received:
2014-05-06
Revised:
2014-06-20
Online:
2015-03-10
Published:
2015-03-10
Contact:
Bing-gan LOU
吴金丹1, 陈乾2, 刘晓曦1, 林福呈1, 高其康3, 楼兵干1,*()
通讯作者:
楼兵干
基金资助:
CLC Number:
Jin-dan WU, Qian CHEN, Xiao-xi LIU, Fu-cheng LIN, Qi-kang GAO, Bing-gan LOU. Preliminary Study on Mechanisms of Growth Promotion in Rice Colonized by Piriformospora indica[J]. Chinese Journal OF Rice Science, 2015, 29(2): 200-207.
吴金丹, 陈乾, 刘晓曦, 林福呈, 高其康, 楼兵干. 印度梨形孢对水稻的促生作用及其机理的初探[J]. 中国水稻科学, 2015, 29(2): 200-207.
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URL: http://www.ricesci.cn/EN/10.3969/j.issn.1001-7216.2015.02.012
Fig. 2. Influence of P. indica on rice seedling growth. A, 20 days after inoculation with P. indica (Left: control; Right: colonized by P. indica); B, 40 days after inoculation with P. indica (Left: control; Right: colonized by P. indica).
基因 Gene | 引物序列 Primer sequences |
---|---|
OsIAA13 | 5'-CCGCAACTGGGAACAATATG-3' |
5'-ACAAGCATCCAGTCACCATC-3' | |
YUCCA | 5'-GATGTGTTCGGTGAAATGCC-3' |
5'-AGCGCCTATGGTTCAGTAATC-3' | |
NRR | 5'-AAGTGTCAGTTCTGGGTGC-3' |
5'-AACCTGAATGTCTCCTTCGC-3' | |
OsUBQ5[ | 5'-GAAGGAGGAGGAAATCGAAC-3' |
(内参基因) | 5'-CTTCAC AGAGGTGATGCTAAGG-3' |
Table 1 Specific primers of growth-related genes for quantitative RT-PCR.
基因 Gene | 引物序列 Primer sequences |
---|---|
OsIAA13 | 5'-CCGCAACTGGGAACAATATG-3' |
5'-ACAAGCATCCAGTCACCATC-3' | |
YUCCA | 5'-GATGTGTTCGGTGAAATGCC-3' |
5'-AGCGCCTATGGTTCAGTAATC-3' | |
NRR | 5'-AAGTGTCAGTTCTGGGTGC-3' |
5'-AACCTGAATGTCTCCTTCGC-3' | |
OsUBQ5[ | 5'-GAAGGAGGAGGAAATCGAAC-3' |
(内参基因) | 5'-CTTCAC AGAGGTGATGCTAAGG-3' |
生物学性状 Biological characteristics | 对照 Control | P. indica处理 P. indica treatment | ||||
---|---|---|---|---|---|---|
株高 Plant height /cm | 16.39 | ± | 1.44 b B | 17.79 | ± | 0.89 a A |
根长 Root length/cm | 10.73 | ± | 1.97 a A | 12.05 | ± | 2.37 a A |
根数 Number of roots | 10.33 | ± | 1.54 a A | 10.20 | ± | 1.15 a A |
最大叶长 Max leaf length/cm | 9.96 | ± | 1.13 b B | 12.13 | ± | 0.99 a A |
最大叶宽 Max leaf width /cm | 0.25 | ± | 0.03 b B | 0.30 | ± | 0.02 a A |
根鲜质量 Root weight/g | 0.0300 | ± | 0.0062 b A | 0.0350 | ± | 0.0063 a A |
地上部鲜质量 Shoot weight/g | 0.0720 | ± | 0.0121 b B | 0.0860 | ± | 0.0154 a A |
根干质量 Dry root weight/g | 0.0036 | ± | 0.0008 a A | 0.0041 | ± | 0.0006 a A |
地上部干质量 Dry shoot weight/g | 0.0091 | ± | 0.0015 b B | 0.0111 | ± | 0.0016 a A |
Table 2 Effect of P. indica on biological characteristics of rice.
生物学性状 Biological characteristics | 对照 Control | P. indica处理 P. indica treatment | ||||
---|---|---|---|---|---|---|
株高 Plant height /cm | 16.39 | ± | 1.44 b B | 17.79 | ± | 0.89 a A |
根长 Root length/cm | 10.73 | ± | 1.97 a A | 12.05 | ± | 2.37 a A |
根数 Number of roots | 10.33 | ± | 1.54 a A | 10.20 | ± | 1.15 a A |
最大叶长 Max leaf length/cm | 9.96 | ± | 1.13 b B | 12.13 | ± | 0.99 a A |
最大叶宽 Max leaf width /cm | 0.25 | ± | 0.03 b B | 0.30 | ± | 0.02 a A |
根鲜质量 Root weight/g | 0.0300 | ± | 0.0062 b A | 0.0350 | ± | 0.0063 a A |
地上部鲜质量 Shoot weight/g | 0.0720 | ± | 0.0121 b B | 0.0860 | ± | 0.0154 a A |
根干质量 Dry root weight/g | 0.0036 | ± | 0.0008 a A | 0.0041 | ± | 0.0006 a A |
地上部干质量 Dry shoot weight/g | 0.0091 | ± | 0.0015 b B | 0.0111 | ± | 0.0016 a A |
穗粒性状 Panicle property | 岳优9113 Yueyou 9113 | 钱优2号 Qianyou 2 | ||
---|---|---|---|---|
-P. indica | +P. indica | -P. indica | +P. indica | |
穗长 Length of panicle/cm | 20.33 b B | 22.34 a A | 18.39 b B | 20.38 a A |
每穗总粒数 Total grains per panicle | 80.92 b B | 111.16 a A | 79.84 b A | 96.52 a A |
每穗实粒数 Filled grains per panicle | 64.12 b B | 86.84 aA | 56.64 b B | 69.04 a A |
结实率 Seed-setting rate/% | 79.24 a A | 78.12 a A | 70.94 a A | 71.53 a A |
千粒重 1000-grain weight/g | 21.62 a A | 22.51 a A | 23.53 a A | 23.81 a A |
Table 3 Effect of P. indica on rice panicle properties.
穗粒性状 Panicle property | 岳优9113 Yueyou 9113 | 钱优2号 Qianyou 2 | ||
---|---|---|---|---|
-P. indica | +P. indica | -P. indica | +P. indica | |
穗长 Length of panicle/cm | 20.33 b B | 22.34 a A | 18.39 b B | 20.38 a A |
每穗总粒数 Total grains per panicle | 80.92 b B | 111.16 a A | 79.84 b A | 96.52 a A |
每穗实粒数 Filled grains per panicle | 64.12 b B | 86.84 aA | 56.64 b B | 69.04 a A |
结实率 Seed-setting rate/% | 79.24 a A | 78.12 a A | 70.94 a A | 71.53 a A |
千粒重 1000-grain weight/g | 21.62 a A | 22.51 a A | 23.53 a A | 23.81 a A |
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