水稻减数分裂期高温对苯丙烷类代谢及下游分支代谢途径的影响

doi:10.16819/j.1001-7216.2023.221112

中国水稻科学 ›› 2023, Vol. 37 ›› Issue (4): 368-378.DOI: 10.16819/j.1001-7216.2023.221112

水稻减数分裂期高温对苯丙烷类代谢及下游分支代谢途径的影响

汪胜勇¹, 陈宇航¹, 陈会丽¹, 黄钰杰¹, 张啸天¹, 丁双成²(), 王宏伟¹()

¹农业农村部长江中游作物绿色高效生产重点实验室(部省共建)/长江大学农学院，湖北荆州434025
²湿地生态与农业利用教育部工程研究中心/长江大学农学院，湖北荆州434025

收稿日期:2022-11-24 修回日期:2023-02-07 出版日期:2023-07-10 发布日期:2023-07-17
通讯作者: *email: shchding@yangtzeu.edu.cn;wanghw@yangtzeu.edu.cn
基金资助:
湿地生态与农业利用教育部工程研究中心开放基金资助项目(KFT202012);主要粮食作物产业化湖北省协同创新中心开放基金资助项目(KFT202108)

Effects of High Temperature on Phenylpropane Metabolism and Downstream Branch Metabolic Pathways in Rice Meiosis

WANG Shengyong¹, CHEN Yuhang¹, CHEN Huili¹, HUANG Yujie¹, ZHANG Xiaotian¹, DING Shuangcheng²(), WANG Hongwei¹()

¹MARA Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River (Co-construction by Ministry and Province)/College of Agriculture, Yangtze University, Jingzhou 434025, China
²Engineering Research Center of Ecology and Agricultural Use of Wetland, Ministry of Education/ College of Agriculture, Yangtze University, Jingzhou 434025, China

Received:2022-11-24 Revised:2023-02-07 Online:2023-07-10 Published:2023-07-17
Contact: *email: shchding@yangtzeu.edu.cn;wanghw@yangtzeu.edu.cn

摘要/Abstract

摘要：

【目的】 探究水稻减数分裂期高温如何影响苯丙烷类代谢，并分析其与水稻耐热性的关系。【方法】 以N22、广陆矮15、SDWG005、全两优681、Y两优900、Y两优1号、两优培九和绵恢101(MH101)等8种耐热和不耐热水稻品种为试验材料，设置常温和高温处理，分析减数分裂期高温胁迫对水稻的花粉活力与苯丙烷类代谢关键酶活性、木质素、总黄酮及总酚等主要代谢产物含量之间的相关性；并进一步选择极端耐高温的SDWG005和极端不耐高温的MH101为材料分析苯丙烷类代谢、碳水化合物代谢和抗氧化系统对水稻耐热性的影响。【结果】 1) 与对照相比，高温显著降低水稻花粉活力和颖花受精率，不同的水稻品种受高温影响后，花粉活力和颖花受精率的降幅不同。2) 高温显著增加颖花中肉桂酸-4-羟化酶和4-香豆酸辅酶A连接酶活性以及木质素、总黄酮和总酚的含量，且耐热品种增幅高于敏感品种。3) 高温下花粉活力与肉桂酸-4-羟化酶活性、木质素含量显著相关，颖花受精率与木质素含量以及木质素含量与类黄酮含量极显著相关。4) 与MH101相比，SDWG005小穗颖壳中木质素受高温显著诱导积累，且高温下能够维持较高细胞壁过氧化物酶活性。5) 与MH101相比，SDWG005颖花在高温下能够维持较高过氧化物酶、超氧化物歧化酶和抗坏血酸氧化酶活性，进而减少颖花中过氧化氢和丙二醛的积累。高温下SDWG005颖花中淀粉含量更高，酸性转化酶、蔗糖合酶及ATPase基因的表达量显著增加。【结论】 减数分裂期高温促进颖花中苯丙烷类代谢关键酶活性的上升和代谢产物含量的增加，耐热品种高温下能够积累较多的木质素和类黄酮，具有较高抗氧化酶活性，同时蔗糖代谢和能量产生效率较高，从而具有较强的耐热性。

关键词: 水稻, 高温, 颖花, 苯丙烷类代谢, 木质素, 总黄酮

Abstract:

【Objective】 Our purposes are to investigate how high temperature during rice meiosis affects phenylpropane metabolism and analyze its relationship with heat tolerance of rice. 【Methods】 Eight rice varieties differed in heat tolerance including N22, GLA15 (Guanglu’ai 15), SDWG005, QLY681 (Quanliangyou 681), YLY900 (Y Liangyou 900), YLY1 (Y Liangyou 1), LYP9 (Liangyoupeijiu), and MH101 (Mianhui 101) were used as experimental materials and exposed to room temperature and high temperature for 5 days. The correlations between activities of key enzymes in phenylpropane metabolism and main metabolite contents such as lignin, total flavonoids and total phenols were analyzed as well as the fertility of rice under high temperature stress during the meiotic stage. SDWG005 (heat resistant) and MH101 (heat sensitive) were used as experimental materials to analyze the effects of phenylpropane metabolism, carbohydrate metabolism and antioxidant defense system on heat tolerance of rice. 【Results】 1) Compared with the control, the pollen vitality and fertilization rate of spikelets decreased significantly to various extents at high temperature. 2) HT(high temperature) significantly increased the activities of cinnamate-4-hydroxylase and 4-coumaric acid coenzyme A ligase, as well as the accumulation of lignin, flavonoids and total phenols in spikelets, with resistant varieties registering a higher growth than sensitive ones. 3) Correlation analysis showed that in response to HT pollen activity was significantly correlated with cinnamate-4-hydroxylase activity and lignin content, spikelet fertility was significantly correlated with lignin content, and lignin content was significantly correlated with flavonoid content. 4) Compared with MH101, lignin accumulation in the glumes of SDWG005 was significantly induced under HT. And higher cell wall peroxidase activities were maintained in SDWG005 under HT. 5) Compared with MH101, SDWG005 could maintain higher antioxidant enzyme activities under HT, resulting in less accumulation of H₂O₂ and malondialdehyde. Under HT, the starch level in SDWG005 flowers was higher, and the expression levels of genes involved in acid invertase, sucrose synthase and ATPase were significantly upregulated. 【Conclusion】 High temperature stress increases the key enzyme activities and metabolite contents in the phenylpropanoid pathway in spikelets during the meiosis stage. The resistant variety accumulates more lignin and flavonoids during HT, has higher antioxidant enzyme activities, higher sucrose metabolism and energy utilization efficiency, thereby improving heat tolerance.

Key words: rice, high temperature, spikelet, phenylpropane metabolism, lignin, flavonoids

汪胜勇, 陈宇航, 陈会丽, 黄钰杰, 张啸天, 丁双成, 王宏伟. 水稻减数分裂期高温对苯丙烷类代谢及下游分支代谢途径的影响[J]. 中国水稻科学, 2023, 37(4): 368-378.

WANG Shengyong, CHEN Yuhang, CHEN Huili, HUANG Yujie, ZHANG Xiaotian, DING Shuangcheng, WANG Hongwei. Effects of High Temperature on Phenylpropane Metabolism and Downstream Branch Metabolic Pathways in Rice Meiosis[J]. Chinese Journal OF Rice Science, 2023, 37(4): 368-378.

图/表 8

图1 常温与高温人工气候室的温度设置

Fig. 1. Temperature in greenhouses for HT and CK.

表1 基因表达分析使用的引物

Table 1. Primers used in gene expression analysis.

基因位点号(基因名) Locus ID(gene name)	正向引物序列 Forward(5'-3')	反向引物 Reverse(5'-3')
LOC_Os09g08072(INV1)	AAGAGCAGGGGTGTACAAG	AAGCTGTGAGTCTGTGGCTC
LOC_Os06g09450(SUS2)	GTGTGCTTGACACCATCCAC	CATGCGGAGACAGGATAACA
LOC_Os05g45420(SnRK1A)	ACAACCAGTGGCTACCTTGG	CGATGATCAGTGGCTGAGTT
LOC_Os07g09610(SnRK1B)	ATATCAGGCGCCGAATACTG	TGTGCCTGAAGAACTTGCTG
LOC_Os05g14550(TOR)	GCTGAACGCTGCAATGACTA	ACCGAACAAGTACTGGAGCA
NC_001320.1(ATPase)	TCGGTGGAGCTACTCTTGGA	CGGGCGCGGATCTATGAATA
Tubulin	TACCGTGCCCTTACTGTTCC	CGGTGGAATGTCACAGACAC

图2 不同水稻品种减数分裂期高温处理下的花粉活力(A)和颖花受精率(B) GLA15－广陆矮15；QLY681－全两优681；LYP9－两优培九；YLY900－Y两优900；YLY1－Y两优1号；MH101－绵恢101。平均值±标准差，n = 3，柱状图上不同小写字母表示不同品种及处理间差异达5%显著水平。下同。

Fig. 2. Pollen vitality (A) and spikelet fertility (B) of different rice cultivars under HT during meiosis stage. GLA15, Guanglu’ai 15; QLY681, Quanliangyou 681; LYP9, Liangyoupeijiu; YLY900, Y Liangyou 900; YLY1, Y Liangyou 1; MH101, Mianhui 101. Mean ± standard deviation, n = 3; different lowercase letters on the bars indicate significant difference among varieties and treatments at 5% level. The same below.

图3 不同水稻品种减数分裂期高温处理下苯丙烷类代谢途径关键酶活性比较

Fig. 3. Comparison of key enzyme activities involved in phenylpropanoid pathway in different rice cultivars under HT during meiosis stage.

图4 不同水稻品种减数分裂期高温处理下颖花木质素、总黄酮和总酚含量的比较

Fig. 4. Comparison of lignin, flavonoids and total phenol contents in different rice cultivars under HT during meiosis stage.

表2 CK和HT处理下苯丙烷类代谢及下游分支代谢途径相关指标相关性分析

Table 2. Correlation analysis of various indexes involved in phenylpropane metabolism and downstream branch metabolic pathways under CK and HT.

相关系数 Correlation coefficient		花粉活力 Pollen vitality	颖花受精率 Spikelet fertility	苯丙氨酸解氨酶活性 Phenylalanine ammonia lyase activity	肉桂酸-4- 羟化酶活性 Cinnamic acid- 4-hydroxylase activity	4-香豆酸辅酶 A连接酶活性 4-Coumaric acid coenzyme A ligase activity	总酚 Total phenols	类黄酮 Flavonoids	木质素 Lignin
CK	花粉活力Pollen viability	1.000
	颖花受精率Spikelet fertility	0.615	1.000
	苯丙氨酸解氨酶活性 Phenylalanine ammonialyase activity	0.193	0.662	1.000
	肉桂酸-4-羟化酶活性 Cinnamic acid-4-hydroxylase activity	0.499	0.505	0.545	1.000
	4-香豆酸辅酶A连接酶活性 4-Coumaric acid coenzyme A ligase activity	0.406	0.634	0.910**	0.648	1.000
	总酚Total phenols	0.446	0.596	0.836**	0.840**	0.930**	1.000
	总黄酮Flavonoids	0.253	0.510	0.872**	0.412	0.832**	0.709*	1.000
	木质素Lignin	0.334	0.576	0.918**	0.632	0.904**	0.864**	0.960**	1.000
HT	花粉活力Pollen vitality	1.000
	颖花受精率Spikelet fertility	0.905**	1.000
	苯丙氨酸解氨酶活性 Phenylalanine ammonialyase activity	0.188	0.499	1.000
	肉桂酸-4-羟化酶活性 Cinnamic acid-4-hydroxylase activity	0.838**	0.694	0.068	1.000
	4-香豆酸辅酶A连接酶活性 4-Coumaric acid coenzyme A ligase activity	0.416	0.403	0.485	0.546	1.000
	总酚Total phenols	0.561	0.629	0.539	0.539	0.693	1.000
	总黄酮Flavonoids	0.562	0.588	0.659	0.406	0.733*	0.662	1.000
	木质素Lignin	0.775*	0.887**	0.649	0.598	0.590	0.668	0.860**	1.000

图5 水稻减数分裂期高温对颖花细胞壁过氧化物酶活性的影响柱状图上不同小写字母表示不同品种及处理间差异达5%显著水平。

Fig. 5. Effects of HT on cell wall peoxidase activities in spikelets during meiosis stage. Different lowercase letters above the bars indicate significant difference among different varieties and treatments at 5% level.

图6 减数分裂期高温对水稻颖花中抗氧化酶活性、过氧化氢、丙二醛和碳水化合物含量以及蔗糖代谢和能量水平相关基因表达的影响柱状图上不同小写字母表示不同品种及处理间差异达5%显著水平。

Fig. 6. Effects of HT on antioxidant enzyme activities, H2O2, malondialdehyde, carbohydrate contents, and gene expression involved in sucrose metabolic and energy level in spikelets during meiosis stage. Different lowercase letters above the bars indicate significant difference among different varieties and treatments at 5% level.

参考文献 52

[1]	Shi P H, Tang L, Wang L H, Sun T, Liu L L, Cao W X, Zhu Y. Post-heading heat stress in rice of south China during 1981-2010[J]. PLoS One, 2015, 10(6): e0130642.
[2]	Shukla P R, Skeg J, Calvo Buendia E, Masson-Delmotte V, Pörtner H O, Malley J. Climate change and land:An IPCC special report on climate change, desertification, land degradation, sustainable land management, food security, and greenhouse gas fluxes in terrestrial ecosystems[M]. Koyoto, Japan: Intergovernmental Panel on Climate Change(IPCC), 2019.
[3]	杨军, 章毅之, 贺浩华, 李迎春, 陈小荣, 边建民, 金国花, 李翔翔, 黄淑娥. 水稻高温热害的研究现状与进展[J]. 应用生态学报, 2020, 31(8): 2817-2830.
	Yang J, Zhang Y Z, He H H, Li Y C, Chen X R, Bian J M, Jin G H, Li X X, Huang S E. Current status and research advances of high-temperature hazards in rice[J]. Chinese Journal of Applied Ecology, 2020, 31(8): 2817-2830. (in Chinese with English abstract)
[4]	田小海, 罗海伟, 吴晨阳. 中国水稻热害研究历史、进展与展望[J]. 中国农学通报, 2009, 25(22): 166-168.
	Tian X H, Luo H W, Wu C Y. Research on heat stress of rice in China: Progress and prospect[J]. Chinese Agricultural Science Bulletin, 2009, 25(22): 166-168. (in Chinese with English abstract)
[5]	Peng S B, Huang J L, Sheehy J E, Laza R C, Cassman K G. Rice yields decline with higher night temperature from global warming[J]. Proceedings of the National Academy of Sciences of the United States of America, 2004, 101(27): 9971-9975.
[6]	Ray D K, Gerber J S, Macdonald G K, West P C. Climate variation explains a third of global crop yield variability[J]. Nature Communications, 2015, 6: 5989.
[7]	Sage T L, Bagha S, Lundsgaard-Nielsen V, Branch H A, Sultmanis S, Sage R F. The effect of high temperature stress on male and female reproduction in plants[J]. Field Crops Research, 2015, 182: 30-42.
[8]	Endo M, Tsuchiya T, Hamada K, Kawamura S, Yano K. High temperatures cause male sterility in rice plants with transcriptional alterations during pollen development[J]. Plant & Cell Physiology, 2009, 50(11): 1911-22.
[9]	Sato S, Peet M M, Thomas J F. Determining critical pre- and post-anthesis periods and physiological processes in Lycopersicon esculentum Mill. exposed to moderately elevated temperatures[J]. Journal of Experimental Botany, 2002, 53(371): 1187-1195.
[10]	Jagadish S V K, Craufurd P Q, Wheeler T R. Phenotyping parents of mapping populations of rice for heat tolerance during anthesis[J]. Crop Science, 2008, 48(3): 1140-1146.
[11]	Wilson Z A, Song J, Taylor B, Yang C Y. The final split: The regulation of anther dehiscence[J]. Journal of Experimental Botany, 2011, 62(5): 1633-1649.
[12]	Matsui T, Omasa K, Horie T. High temperature at flowering inhibits swelling of pollen grains, a driving force for thecae dehiscence in rice (Oryza sativa L.)[J]. Plant Production Science, 2000, 3(4): 430-434.
[13]	Matsui T, Omasa K. Rice cultivars tolerant to high temperature at flowering: Anther characteristics[J]. Annals of Botany, 2002, 89(6): 683-687.
[14]	Prasad P V V, Boote K J, Allen L H, Sheehy J E. Species, ecotype and cultivar differences in spikelet fertility and harvest index of rice in response to high temperature[J]. Field Crops Research, 2006, 95(2): 398-411.
[15]	Jagadish S V, Muthurajan R, Oane R, Wheeler T R, Heuer S, Bennett J, Craufurd P Q. Physiological and proteomic approaches to address heat tolerance during anthesis in rice (Oryza sativa L.)[J]. Journal of Experimental Botany, 2010, 61(1): 143-156.
[16]	Kobayashi K, Matsui T, Murata Y, Yamamoto M. Percentage of dehisced thecae and length of dehiscence control pollination stability of rice cultivars[J]. Plant Production Science, 2011, 14(2): 89-95.
[17]	张桂莲, 张顺堂, 肖浪涛, 唐文帮, 肖应辉, 陈立云. 抽穗开花期高温胁迫对水稻花药、花粉粒及柱头生理特性的影响[J]. 中国水稻科学, 2014, 28(2): 155-166.
	Zhang G L, Zhang S T, Xiao L T, Tang W B, Xiao Y H, Chen L Y. Effect of high temperature stress on physiological characteristics of anther, pollen and stigma of rice during heading-flowering stage[J]. Chinese Journal of Rice Science, 2014, 28(2): 155-166. (in Chinese with English abstract)
[18]	Wahid A, Gelani S, Ashraf M, Foolad M R. Heat tolerance in plants: An overview[J]. Environmental and Experimental Botany, 2007, 61(3): 199-223.
[19]	Locato V, de Pinto M C, de Gara L. Different involvement of the mitochondrial, plastidial and cytosolic ascorbate-glutathione redox enzymes in heat shock responses[J]. Physiologia Plantarum, 2009, 135(3): 296-306.
[20]	Locato V, Gadaleta C, de Gara L, de Pinto M C. Production of reactive species and modulation of antioxidant network in response to heat shock: A critical balance for cell fate[J]. Plant Cell & Environment, 2008, 31(11): 1606-1619.
[21]	Parish R W, Phan H A, Iacuone S, Li S F. Tapetal development and abiotic stress[J]. Functional Plant Biology, 2012, 39(7): 553-559.
[22]	De Storme N, Geelen D. The impact of environmental stress on male reproductive development in plants: Biological processes and molecular mechanisms[J]. Plant Cell & Environment, 2014, 37(1): 1-18.
[23]	Bahuguna R N, Solis C A, Shi W J, Jagadish K S. Post-flowering night respiration and altered sink activity account for high night temperature-induced grain yield and quality loss in rice (Oryza sativa L.)[J]. Physiologia Plantarum, 2017, 159(1): 59-73.
[24]	Herrmann K M, Weaver L M. The Shikimate Pathway[J]. Annual Review of Plant Physiology and Plant Molecular Biology, 1999, 50: 473-503.
[25]	Schubert W J, Acerbo S N. The conversion of D-glucose into lignin in Norwegian spruce[J]. Archives of Biochemistry and Biophysics, 1959, 83(1): 178-82.
[26]	Rogers L A, Dubos C, Cullis I F, Surman C, Poole M, Willment J, Mansfield S D, Campbell M M. Light, the circadian clock, and sugar perception in the control of lignin biosynthesis[J]. Journal of Experimental Botany, 2005, 56(416): 1651-63.
[27]	Jiao Y, Gong X, Qi K, Xie Z, Wang Y, Yuan K, Pan Q, Zhang S, Shiratake K, Khanizadeh S, Tao S. Transcriptome analysis provides new ideas for studying the regulation of glucose-induced lignin biosynthesis in pear calli[J]. BMC Plant Biology, 2022, 22(1): 310.
[28]	Poovaiah C R, Mazarei M, Decker S R, Turner G B, Sykes R W, Davis M F, Stewart C N, Jr. Transgenic switchgrass biomass is increased by overexpression of switchgrass sucrose synthase (PvSUS1)[J]. Biotechnology Journal, 2015, 10(4): 552-563.
[29]	Dong N Q, Lin H X. Contribution of phenylpropanoid metabolism to plant development and plant-environment interactions[J]. Journal of Integrative Plant Biology, 2021, 63(1): 180-209.
[30]	Muhlemann J K, Younts T L B, Muday G K. Flavonols control pollen tube growth and integrity by regulating ROS homeostasis during high-temperature stress[J]. Proceedings of the National Academy of Sciences of the United States of America, 2018, 115(47): e11188-e11197.
[31]	Zhang D B, Wilson Z A. Stamen specification and anther development in rice[J]. Chinese Science Bulletin, 2009, 54(14): 2342-2353.
[32]	Fecht-Christoffers M M, Fuhrs H, Braun H P, Horst W J. The role of hydrogen peroxide-producing and hydrogen peroxide-consuming peroxidases in the leaf apoplast of cowpea in manganese tolerance[J]. Plant Physiology, 2006, 140(4): 1451-63.
[33]	Dong N Q, Sun Y, Guo T, Shi C L, Zhang Y M, Lin H X. UDP-glucosyltransferase regulates grain size and abiotic stress tolerance associated with metabolic flux redirection in rice[J]. Nature Communications, 2020, 11(1): 2629.
[34]	Liu Y Y, Ge Y H, Bi Y, Li C Y, Deng H W, Hu L G, Dong B. Effect of postharvest acibenzolar-S-methyl dipping on phenylpropanoid pathway metabolism in muskmelon fruits[J]. Scientia Horticulturae, 2014, 168: 113-119.
[35]	张水明, 龚凌燕, 曹丹琴, 张永娟, 杨健. 石榴种皮总木质素含量及PgCOMT基因的克隆与表达[J]. 热带亚热带植物学报, 2015, 23(1): 65-73.
	Zhang S M, Gong L Y, Cao D Q, Zhang Y J, Yang J. Total lignin content in pomegranate seed coat and cloning andexpression analysis of PgCOMT gene[J]. Journal of Tropical and Subtropical Botany, 2015, 23(1): 65-73. (in Chinese with English abstract)
[36]	黄晓彤, 史锐, 刘苗苗, 丛龙娇, 刘斯文, 王琪瑶. 同属不同种桑叶总黄酮的含量测定及分析[J]. 亚太传统医药, 2022, 18(8): 91-95.
	Huang X T, Shi R, Liu M M, Cong L J, Liu S W, Wang Q Y. Optimization of extraction process and content determination of total flavonoids from different mulberry leaves[J]. Asia-Pacific Traditional Medicine, 2022, 18(8): 91-95. (in Chinese with English abstract)
[37]	王成章, 李建华, 郭玉霞, 方丽云, 高永革. 光周期对不同秋眠型苜蓿SOD、POD活性的影响[J]. 草地学报, 2007, 15(5): 407-411. (in Chinese with English abstract)
	Wang C Z, Li J H, Guo Y X, Fang L Y, Gao Y G. Effect of photoperiod on SOD and POD activities in alfalfa varieties with different fall dormancy[J]. Acta Agrestia Sinica, 2007, 15(5): 407-411.
[38]	Nakano Y, Asada K. Hydrogen peroxide is scavenged by ascorbate-specificperoxidase in spinach chloroplasts[J]. Plant and Cell Physiology, 1981, 22(5): 867-880.
[39]	李光彦. 能量代谢影响水稻耐热性的作用机理[D]. 武汉: 华中农业大学, 2021.
	Li G Y. The mechanism of energy metabolism mediating rice heat resistance[D]. Wuhan: Huangzhong Agricultural University, 2021. (in Chinese with English abstract)
[40]	刘清泉. 铜胁迫下水稻木质素合成的响应机制及水稻漆酶在植物重金属耐性中的作用[D]. 南京: 南京农业大学, 2015.
	Liu Q Q. Response mechanism of lignin synthesis in rice under copper stress and the role of rice in plants tolerance to heavy metal[D]. Nanjing: Nanjing Agricultural University, 2015. (in Chinese with English abstract)
[41]	崔莹莹, 王晓玲. 水稻产量相关性状QTL的遗传研究进展[J]. 江苏农业科学, 2017, 45(13): 1-7.
	Cui Y Y, Wang X L. Genetic research progress of QTL related traits in rice yield[J]. Jiangsu Agricultural Sciences, 2017, 45(13): 1-7. (in Chinese)
[42]	张桂莲, 张顺堂, 肖浪涛, 武小金, 肖应辉, 陈立云. 花期高温胁迫对水稻花药生理特性及花粉性状的影响[J]. 作物学报, 2013, 39(1): 177-183.
	Zhang G L, Zhang S T, Xiao L T, Wu X J, Xiao Y H, Chen L Y. Effect of high temperature stress on physiological characteristics of anther and pollen traits of rice at flowering stage[J]. Acta Agronomica Sinica, 2013, 39(1): 177-183. (in Chinese with English abstract)
[43]	Oshino T, Abiko M, Saito R, Higashitani A. Premature progression of anther early developmental programs accompanied by comprehensive alterations in transcription during high-temperature in barley plants[J]. Molecular Genetics and Genomics, 2007, 278(1): 31-42.
[44]	Feng B H, Zhang C X, Chen T T, Zhang X F, Tao L X, Fu G F. Salicylic acid reverses pollen abortion of rice caused by heat stress[J]. BMC Plant Biology, 2018, 18(1): 245.
[45]	Fraser C M, Chapple C. The phenylpropanoid pathway in Arabidopsis[J]. Arabidopsis Book, 2011, 9: e0152.
[46]	Dixon R A. Natural products and plant disease resistance[J]. Nature, 2001, 411(6839): 843-847.
[47]	Mishra A K, Baek K H. Salicylic acid biosynthesis and metabolism: A divergent pathway for plants and bacteria[J]. Biomolecules, 2021. 11(5): 705.
[48]	陈爱国, 彭东, 陈向东, 石屹, 梁洪波. 烤烟苯丙烷代谢中相关酶活性和多酚产物的关系研究[C]. 山东植物生理学会第七次代表大会暨植物生物学与现代农业研讨会, 济南: 山东省科学技术协会, 2012: 240-246.
	Chen A G, Peng D, Chen X D, Shi Y, Liang H B. Studies on Relationships of Related Enzyme Activities and Polyphenol Products of Phenylpropanoid Metabolic Pathway in Flue-cured tobacco[C]. Proceedings of the 7th Congress of Shandong Plant Physiology Society and Symposium on Plant Biology and Modern Agriculture, Jinan: Shandong Science and technology Association, 2012: 240-246. (in Chinese with English abstract)
[49]	Zhang C X, Feng B H, Chen T T, Fu W M, Li H B, Li G Y, Jin Q Y, Fu G F. Heat stress-reduced kernel weight in rice at anthesis is associated with impaired source-sink relationship and sugars allocation[J]. Environmental and Experimental Botany, 2018, 155: 718-733.
[50]	Baena-González E, Hanson J. Shaping plant development through the SnRK1-TOR metabolic regulators[J]. Current Opinion in Plant Biology, 2017, 35: 152-157.
[51]	Yu P H, Jiang N, Fu W M, Zheng G J, Li G Y, Feng B H, Chen T T, Ma J Y, Li H B, Tao L X, Fu G F. ATP hydrolysis determines cold tolerance by regulating available energy for glutathione synthesis in rice seedling plants[J]. Rice, 2020, 13(1): 23.
[52]	Li G Y, Zhang C X, Zhang G H, Fu G F. Abscisic acid negatively modulates heat tolerance in rolled leaf rice by increasing leaf temperature and regulating energy homeostasis[J]. Rice, 2020, 13(1): 18.

水稻减数分裂期高温对苯丙烷类代谢及下游分支代谢途径的影响

Effects of High Temperature on Phenylpropane Metabolism and Downstream Branch Metabolic Pathways in Rice Meiosis

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

图/表 8

参考文献 52

相关文章 15

编辑推荐

Metrics

[1]	兰金松, 庄慧. 水稻株型的分子机理研究进展 [J]. 中国水稻科学, 2023, 37(5): 449-458.
[2]	李景芳, 温舒越, 赵利君, 陈庭木, 周振玲, 孙志广, 刘艳, 陈海元, 张云辉, 迟铭, 邢运高, 徐波, 徐大勇, 王宝祥. 基于CRISPR/Cas9技术创制耐盐香稻 [J]. 中国水稻科学, 2023, 37(5): 478-485.
[3]	黄奇娜, 徐有祥, 林光号, 党洪阳, 郑振权, 张燕, 王晗, 邵国胜, 尹献远. 硅对镉胁迫下水稻苗期抗氧化酶系统及镉离子吸收和转运相关基因表达水平的影响 [J]. 中国水稻科学, 2023, 37(5): 486-496.
[4]	徐欢, 周涛, 孙悦, 王木妹, 杨亚春, 马卉, 李浩, 徐大伟, 周海, 杨剑波, 倪金龙. 水稻颖壳类病斑突变体glmm1的鉴定与基因定位 [J]. 中国水稻科学, 2023, 37(5): 497-506.
[5]	姚晓云, 陈春莲, 熊运华, 黄永萍, 彭志勤, 刘进, 尹建华. 水稻加工和外观品质性状QTL鉴定 [J]. 中国水稻科学, 2023, 37(5): 507-517.
[6]	袁沛, 周旋, 杨威, 尹凌洁, 靳拓, 彭建伟, 荣湘民, 田昌. 化肥减氮配施对洞庭湖区双季稻产量和田面水氮磷流失风险的影响 [J]. 中国水稻科学, 2023, 37(5): 518-528.
[7]	肖大康, 胡仁, 韩天富, 张卫峰, 侯俊, 任科宇. 氮肥用量和运筹对我国水稻产量及其构成因子影响的整合分析 [J]. 中国水稻科学, 2023, 37(5): 529-542.
[8]	夏杨, 李传明, 刘琴, 韩光杰, 徐彬, 黄立鑫, 祁建杭, 陆玉荣, 徐健. 印度梨形孢对盐胁迫下水稻幼苗生长及抗氧化系统的影响 [J]. 中国水稻科学, 2023, 37(5): 543-552.
[9]	任志奇, 薛可欣, 董铮, 李小湘, 黎用朝, 郭玉静, 刘文强, 郭梁, 盛新年, 刘之熙, 潘孝武. 水稻外卷叶突变体ocl1的鉴定及基因定位[J]. 中国水稻科学, 2023, 37(4): 337-346.
[10]	肖乐铨, 李雷, 戴伟民, 强胜, 宋小玲. 转cry2A/bar*基因水稻与杂草稻杂交后代的苗期生长特性[J]. 中国水稻科学, 2023, 37(4): 347-358.
[11]	李刚, 高清松, 李伟, 张雯霞, 王健, 程保山, 王迪, 高浩, 徐卫军, 陈红旗, 纪剑辉. 定向敲除SD1基因提高水稻的抗倒性和稻瘟病抗性[J]. 中国水稻科学, 2023, 37(4): 359-367.
[12]	董立强, 杨铁鑫, 李睿, 商文奇, 马亮, 李跃东, 隋国民. 株行距配置对超高产田水稻产量及根系形态生理特性的影响[J]. 中国水稻科学, 2023, 37(4): 392-404.
[13]	韩聪, 何禹畅, 吴丽娟, 郏丽丽, 王磊, 鄂志国. 水稻碱性亮氨酸拉链(bZIP)蛋白家族功能研究进展[J]. 中国水稻科学, 2023, 37(4): 436-448.
[14]	沈雨民, 陈明亮, 熊焕金, 熊文涛, 吴小燕, 肖叶青. 水稻内外稃异常发育突变体blg1 (beak like grain 1)的表型分析与精细定位[J]. 中国水稻科学, 2023, 37(3): 225-232.
[15]	段敏, 谢留杰, 高秀莹, 唐海娟, 黄善军, 潘晓飚. 利用CRISPR/Cas9技术创制广亲和水稻温敏雄性不育系[J]. 中国水稻科学, 2023, 37(3): 233-243.