氮高效水稻主要源库性状的基本特点及其调控

doi:10.16819/j.1001-7216.2017.6127

摘要/Abstract

摘要：

【目的】本研究皆在阐明氮高效水稻源库性状的基本特点。【方法】在大田条件下,于2012–2014年设计了两个试验。2012年和2013年,以染色体单片段代换系遗传群体114个水稻株系为供试材料,依据成熟期吸氮量和产量两个性状将114个株系群体分为6种不同氮效率类型。2014年,在前2两年试验的基础上,以筛选出的氮高效株系（L68）和氮低效株系（L2）为供试材料,研究施氮量对两种氮效率水稻株系产量、源库性状的影响。【结果】1）114个株系群体成熟期吸氮量和产量差异均较大,吸氮量变幅为11.53~27.66 g/m²,产量变幅为311.74~763.35 kg/666.7 m²,随着吸氮量的增加,产量呈上升趋势。产量类型与吸氮量类型并不完全一致,高吸氮量是高产的重要基础,但产量还可能受到其他因素的影响;2）氮高效水稻抽穗期叶面积系数（包括有效叶面积、高效叶面积、总叶面积）、成熟期叶面积系数均显著大于氮低效水稻,叶面积构成因子中氮高效水稻绿叶质量明显高于氮低效水稻,但比叶重不同氮效率品种间差异较小;3）氮高效水稻单位面积库容量、单位面积颖花量显著高于氮低效水稻,氮高效水稻单位干质量、单位叶面积和单位氮素库容量大,库容形成能力强;4）氮高效水稻单位叶面积颖花数、单位叶面积籽粒产量大,结实期净同化率高,氮高效水稻“流”畅,叶片光合能力强;5）综合分析表明,库容量对氮素高效吸收影响较大。提高单位氮素库容量有助于提高单位面积库容量。不同施氮水平下,氮高效水稻叶面积系数、库容量、吸氮量和产量均明显高于氮低效水稻,叶面积系数在低氮水平下两者差异最大,其他3个指标以低、中氮差异较大。【结论】氮高效水稻源库指标均优于其他类型,且这一优势在不同施氮量亦是如此。

关键词: 氮高效水稻, 施氮量, 吸氮量, 产量, 源库

Abstract:

【Objective】In order to reveal the features of the source-sink in N-efficient rice,【Method】we conducted two experiments from 2012 to 2014 in paddy fields. A chromosome single segment substitution lines (CSSSLs) consisted of 114 rice lines were cultured in 2012 and 2013. On the basis of plant nitrogen accumulation per area and yield per area at maturity, the rice cultivars were clustered into six types by MinSSw (Minimum Square Sum Within Groups) method. We selected two lines, one is featured with high nitrogen accumulation and high yield (L68, nitrogen-efficient), the other with low nitrogen accumulation and low yield (L2, nitrogen-inefficient). The effects of nitrogen application levels on yield and source-sink characters were studied. 【Result】1) There was a significant difference among all tested lines in N accumulation per unit area and yield per unit area, the variation of N accumulation per unit area ranged from 11.53 to 27.66 g/m², the variation of yield per unit area from 311.74 to 763.35 kg/666.7 m². With the increase of nitrogen accumulation, grain yield tended to increase, but nitrogen accumulation types and the yield types were not completely similar. High nitrogen accumulation is an important basis of high yield, but yield was also affected by other factors; 2) The LAI at heading stage (including valid, efficient and total LAI) and mature stage of the N-efficient rice were higher than that of the N-inefficient. The green leaf weight which is the components of leaf area of N-efficient rice was higher than that of N-inefficient rice, but the specific leaf weight had little difference in different N-efficiency rice. 3) The storage capacity per unit area and spikelet number per unit area of N-efficient rice were higher than those of N-inefficient rice. The storage capacity per unit dry weight, storage capacity per unit leaf area, storage capacity per unit nitrogen absorption of N-efficient rice were large, and their ability of developing storage capacity was strong; 4) The spikelet numbers per unit leaf area, grain yield per unit leaf area and net assimilation rate at seed setting stage of N-efficient rice were large, and their “flux” was fluent and the photosynthetic capability was strong; 5) Comprehensive analysis shows that, the storage capacity had a large influence on the nitrogen efficient absorption, and the increasing storage capacity per unit nitrogen absorption contributed to increase in the storage per unit area. Under different nitrogen application levels, the LAI, storage capacity, N absorption and yield of N-efficient rice were higher than those of N-inefficient rice significantly. Under low nitrogen level, the LAI difference between two different N efficiency rice was maximum, nevertheless the differences of the other three indicators were larger under condition of low and medium nitrogen. 【Conclusion】The source-sink properties of N-efficient rice are better than other types at different N application levels.

Key words: N-efficient rice, N application level, N accumulation, yield, source-sink

陈琛, 张家星, 李万元, 唐东南, 罗刚, 王祥菊, 莫兰婧, 吕旻珈, 周娟, 梁国华, 黄建晔, 王余龙, 姚友礼, 董桂春. 氮高效水稻主要源库性状的基本特点及其调控[J]. 中国水稻科学, 2017, 31(2): 185-194.

Chen CHEN, Jiaxing ZHANG, Wanyuan LI, Dongnan TANG, Gang LUO, Xiangju WANG, Lanjing MO, Minjia LÜ, Juan ZHOU, Guohua LIANG, Jianye HUANG, Yulong WANG, Youli YAO, Gui-chun DONG. Fundamental Features of Source-Sink Characters and Their Regulation in High Nitrogen Efficiency Rice Lines[J]. Chinese Journal OF Rice Science, 2017, 31(2): 185-194.

图/表 10

参考文献 35

[1]	凌启鸿, 张洪程, 蔡建中, 苏祖芳, 凌励. 水稻高产群体质量及其优化控制探讨. 中国农业科学, 1993, ,26(6): 1-11
	Ling Q H, Zhang H C, Cai J Z, Su Z F, Ling L.High-yielding rice population quality and its optimization control.China Agric Sin, 1993,26(6): 1-11.
[2]	屠乃美, 官春云. 水稻幼穗分化期间减源对源库关系的影响. 湖南农业大学学报, 1999, 56(6): 430-436.
	Tu N M, Guan C Y.Effects of leaf-cutting treatments on source-sink relation of rice during panicle initiation.J Hunan Agric Univ, 1999, 56(6): 430-436.
[3]	黄育明. 我国水稻品种改良过程源库特征的变化. 福建农业大学学报, 1998, 27(3): 271-278.
	Huang Y M.Changes of sink-source characteristics during the cultivar improvement in rice in China.J Fujian Agric Univ, 1998, 27(3): 27l-278.
[4]	凌启鸿. 作物群体质量. 上海: 上海科学技术出版社, 2000: 42-120.
	Ling Q H.Crop population quality. Shanghai: Shanghai Scientific and Technical Publishers, 2000: 42-120.
[5]	张岳芳, 王余龙, 陈留根. 不同氮素累积量类型籼稻品种有关源库指标的基本特点. 江西农业学报, 2008(6): 1-4, 9.
	Zhang Y F, Wang Y L, Chen L G.Study on main indexes of source and Sink in different nitrogen accumulation types ofindica rice. Acta Agric Jiangxi, 2008(6): 1-4, 9.
[6]	于小凤. 氮素高效吸收型水稻的基本特点. 扬州:扬州大学, 2012
	Yu X F.Fundamental characteristics of conventional japonica rice cultivars with high nitrogen uptake efficiency. Yangzhou: Yangzhou University, 2012.
[7]	袁秋梅. 氮肥处理对不同氮素吸收类型水稻品种产量及根系性状的影响. 扬州: 扬州大学, 2013.
	Yuan Q M.The effect of nitrogen fertilization treatments on the grain yield and root traits of indica rice varieties with different nitrogen uptake efficiency. Yangzhou: Yangzhou University, 2013.
[8]	熊洁. 水稻源库结构、稻米品质以及对氮响应的基因型差异. 南京: 南京农业大学, 2011.
	Xiong J.The genetic differences in source-sink structure, rice quality and responses to nitrogen. Nanjing: Nanjing Agricultural University, 2011.
[9]	凌启鸿. 作物群体质量. 上海: 上海科技出版社, 2000: 12-209.
	Ling Q H.Crop population quality. Shanghai: Shanghai Scientific and Technical Publisher, 2000: 12-209. (in Chinese with English abstract)
[10]	韦善清, 徐世宏, 李如平, 郎宁, 江立庚, 董登峰, 张平刚, 陈念平, 秦华东, 陆福勇. 免耕抛栽水稻源库特性研究. 中国农学通报, 2005, 21(9): 237-241.
	Wei S Q, Xu S H, Li R P, Lang N, Jiang L G, Dong D F, Zhang P G, Chen N P, Qin H D, Lu F Y.Studies on source-sink characteristics of no-tillage and cast transplanting rice.Chin Agric Sci Bull, 2005, 21(9): 237-241. (in Chinese with English abstract).
[11]	顾世梁, 莫惠栋. 动态聚类的一种新方法—最小组内平方和法. 江苏农学院学报, 1989, 10(4): 1-8.
	Gu S L, Mo H D.A new dynamic clustering method Sum of squares method within the team.Jiangsu Agric Res, 1989, 10(4): 1-8. (in Chinese with English abstract)
[12]	向秀媛, 刘强, 彭建伟, 荣湘民, 柴慧清, 周旋. 不同地力和养分管理模式对早稻产量和氮肥利用率的影响. 湖南农业科学, 2011(11): 79-81.
	Xiang X Y, Liu Q, Peng J W, Rong X M, Chai H Q, Zhou X.Impacts of different soil fertility levels and nutrient management models on yield and nitrogen Use efficiency of early-rice.Hunan Agric Sc, 2011(11): 79-81. (in Chinese)
[13]	王伟妮, 鲁剑巍, 鲁明星, 李小坤, 李云春, 李慧. 湖北省早、中、晚稻施氮增产效应及氮肥利用率研究. 植物营养与肥料学报, 2011, 17(03):545-553.
	Wang W N, Lu J W, Lu M X, Li X K, Li Y C, Li H.Effect of nitrogen fertilizer application and nitrogen use efficiency of early, middle and late rice in Hubei Province.Plant Nutr Fert Sci, 2011, 17(3): 545-553. (in Chinese with English abstract)
[14]	王琳, 谢树果, 竭润生, 杜晓秋, 何平, 彭伟. 不同施氮量对川东北地区水稻产量及氮肥利用率的影响. 耕作与栽培, 2014(6): 12-16.
	Wang L, Xie S G, Jie R S, Du X Q, He P, Peng W.Effect of different nitrogen fertilizer rate on yield and nitrogen fertilizer utilization rate of rice in Northeast of Sichuan. Till Cult, 2014(6): 12-16. (in Chinese)
[15]	陈琛, 羊彬, 朱正康, 曹文雅, 罗刚, 周娟, 王祥菊, 于小凤, 袁秋梅, 仲军, 王熠, 黄建晔, 王余龙, 董桂春. 影响水稻遗传群体株系氮素高效吸收的主要根系性状. 中国水稻科学, 2015, 29(4): 390-398.
	Chen C, Yang B, Zhu Z K, Cao W Y, Luo G, Zhou J, Wang X J, Yu X F, Yuan Q M, Zhong J, Wang Y, Huang J Y, Wang Y L, Dong G C.Root traits affecting nitrogen efficient absorption in rice genetic populations.Chin J Rice Sci, 2015, 29(4): 390-398. (in Chinese with English abstract)
[16]	叶全宝, 张洪程, 魏海燕, 张瑛, 汪本福, 夏科, 霍中洋, 戴其根, 许轲. 不同土壤及氮肥条件下水稻氮利用效率和增产效应研究. 作物学报, 2005, 31(11): 38-44.
	Ye Q B, Zhang H C, Wei H Y, Zhang Y, Wang B F, Xia K, Huo Z Y, Dai Q G, Xu K.Effects of nitrogen fertilizer on nitrogen use efficiency and yield of rice under different soil conditions.Acta Agron Sin, 2005, 31(11): 38-44. (in Chinese with English abstract)
[17]	陈晓波, 邹文广, 饶鸣钿, 卓伟. 施氮量对杂交早稻T78优2155产量形成和氮素利用的影响. 热带作物学报, 2012, 33(12): 2161-2165.
	Chen X B, Zou W G, Rao M D, Zhuo W.Effects of nitrogen application amount on the yield formation and nitrogen utilization in spring hybrid riceT78 You 2155. Chin J Trop Crops, 2012, 33(12): 2161-2165. (in Chinese with English abstract)
[18]	潘壮. 不同高产水稻基因型氮效率评价.武汉: 华中农业大学, 2012.
	Pan Z.Evaluation for nitrogen high-yielding use rice efficiency of different genotypes. Wuhan: Huazhong Agricultural University, 2012.
[19]	刘桃菊, 朱冰, 江绍琳, 潘星哲, 唐建军, 张笑东, 胡雯君. 施氮量对双季稻氮素吸收和产量的影响及其优化. 浙江农业学报, 2014, 26(4): 1004-1009.
	Liu T J, Zhu B, Hong S L, Pan X Z, Tang J J, Zhang X D, Hu W J.Effect of nitrogen rates on N uptake and use efficiency in double season rice and optimization of nitrogen application.Acta AgricZhejiang, 2014, 26(4):1004-1009. (in Chinese with English abstract)
[20]	蔡桂青. 施氮量和种植密度对水稻产量及氮肥利用率的影响及差异. 长沙:湖南农业大学, 2014.
	Cai G Q.N application and planting density on the effects of cropping yield and nitrogen utilization[D]. Changsha: Hunan Agricultural University, 2014.
[21]	李洪顺. 氮素运筹对超级杂交水稻氮吸收利用与产量的影响.长沙:中南大学, 2010.
	Li H S.Effect of Nitrogen management on absorption and utilization of nitrogen and yield of super hybrid rice. Changsha: Central South University, 2010.
[22]	陆旭, 杨志新, 续勇波, 何霞红, 朱有勇. 不同氮肥用量对元阳梯田水稻产量及构成因素的影响. 云南农业大学学报:自然科学版, 2011, 26(03): 376-381, 388.
	Lu X, Yang Z X, Xu Y B, He X H, Zhu Y Y.Effects of different nitrogen fertilizer use level on yield component and grain yield in Yuanyang terrace.Journal of Yunnan Agric Univ: Nat Sci, 2011, 26(03):376-381, 388. (in Chinese with English abstract)
[23]	董桂春, 王余龙, 张岳芳, 陈培峰, 杨连新, 黄建晔. 不同氮素籽粒生产效率类型籼稻品种产量及其构成的基本特点. 作物学报, 2006, 32(10): 1511-1518.
	Dong G C, Wang Y L, Zhang Y F, Chen P F, Yang L X, Huang J Y.Characteristics of yield and yield components in conventionalindica rice cultivars with different nitrogen use efficiencies for grain output. Acta Agron Sin, 2006, 32(10): 1511-1518. (in Chinese with English abstract)
[24]	张洪程, 马群, 杨雄, 李敏, 葛梦婕, 李国业, 戴其根, 霍中洋, 许轲, 魏海燕, 高辉, 刘艳阳. 水稻品种氮肥群体最高生产力及其增长规律. 作物学报, 2012, 38(1): 86-98.
	Zhang H C, Ma Q, Yang X, Li M, Ge M J, Li G Y, Dai Q G, Huo Z Y, Xu K, Wei H Y, Gao H, Liu Y Y.The highest population productivity of nitrogen fertilization and its variation rules in rice cultivars.Acta Agron Sin, 2012, 38(1): 86-98. (in Chinese with English abstract)
[25]	陈莹. 不同水稻品种对施氮水平的响应及减少氨排放效应研究. 南京:南京林业大学, 2013.
	Chen Y.Mechanism of different rice varieties response to nitrogen and effect of reducing ammonia emission. Nanjing: Nanjing Forestry University, 2013.
[26]	董桂春, 陈琛, 袁秋梅, 羊彬, 朱正康, 曹文雅, 仲军, 周娟, 罗刚, 王熠, 黄建晔, 王余龙. 氮肥处理对氮素高效吸收水稻根系性状及氮肥利用率的影响. 生态学报, 2016, 36(3): 642-651.
	Dong G C, Chen C, Yuan Q M, Yang B, Zhu Z K, Cao W Y, Zhong J, Zhou J, Luo G, Wang Y, Huang J Y, Wang Y L.The effect of nitrogen fertilizer treatments on root traits and nitrogen use efficiency inindica rice varieties with high nitrogen absorption efficiency. Acta Ecol Sin, 2016, 36(3): 642-651. (in Chinese with English abstract)
[27]	张俊国, 张三元, 杨春刚,郭桂珍, 孙强. 水稻氮高效品种资源筛选的初步研究Ⅰ:不同施氮水平下水稻产量和干物质生产的品种间差异. 吉林农业科学, 2008, 33(6): 7-10.
	Zhang J G, Zhang S Y, Yang C G, Guo G Z, Sun Q.Preliminary studies on selection of high nitrogen utilization rice variety resourcesⅠ: Differences of yield and dried mass production among rice varieties under different nitrogen application rate.J Jilin Agric Sci, 2008, 33(6): 7-10. (in Chinese with English abstract)
[28]	Tadahiko M.Plant Nutrition for Sustainable Food Production and Environment. Netherlands: Springer Science& Business Media, 1997.
[29]	石庆华, 徐益群, 张佩莲, 曾宪江, 钟旭华, 潘晓华. 籼粳杂交稻的氮素吸收特性及其对“库”“源”特征的影响. 杂交水稻, 1995, 10(4): 19-22.
	Shi Q H, Xu Y Q, Zhang P L, Zeng X J, Zhong X H, Pan X H.Nitrogen absorption properties ofindica-japonica hybrid rice and its influence on library source characteristics. Hybrid rice, 1995, 10(4): 19-22. (in Chinese)
[30]	张岳芳. 不同氮素累积量类型籼稻品种的基本特点及其对供氮浓度的响应. 扬州: 扬州大学, 2006.
	Zhang Y F.Fundamental traits of indica rice varieties (Oryza sativa L.) with different nitrogen accumulation capacity and its responses to nitrogen supplying levels. Yangzhou: Yangzhou University, 2006. (in Chinese with English abstract)
[31]	张燕. 氮肥处理对不同氮效率类型籼稻品种产量、氮素吸收利用等性状的影响. 扬州: 扬州大学, 2011.
	Zhang Y.The effect of nitrogen fertilizer treatments on the grain yield, nitrogen absorption and utilization and other traits of indica rice varieties with different nitrogen use. Yangzhou: Yangzhou University, 2011. (in Chinese with English abstract)
[32]	Venkateswarlu B, VisperasR M. Source-sink relationships in cropplants: A review. IRRIResearch Paper Series, Manila, Philippines:The International rice research institute, 1987, 2: 125.
[33]	董桂春, 于小凤, 董燕萍, 李进前, 田昊, 周娟, 王云霞, 杨连新, 黄建晔, 王余龙. 不同库容量类型常规籼稻品种氮素吸收与分配的差异. 中国农业科学, 2009, 42(10): 3432-3441.
	Dong G C, Yu X F, Dong Y P, Li J Q, Tian H, Zhou J, Wang Y X, Yang L X, Huang J Y, Wang Y L.A difference in nitrogen uptake and distribution in conventional indica rice cultivars with different sink-potentials. Sci Agric Sin, 2009, 42(10): 3432-3441. (in Chinese with English abstract)
[34]	单玉华, 王海候, 龙银成, 王余龙, 潘学彪. 不同库容量类型水稻在氮素吸收利用上的差异. 扬州大学学报, 2004, 25(1): 41-45.
	Shan Y H, Wang H H, Long Y C, Wang Y L, Pan X B.Differences of nitrogen uptake and utilization in rice lines with various sink potentials.J Yangzhou Univ, 2004, 25(1): 1-45. (in Chinese with English abstract)
[35]	于小凤, 王熠, 袁秋梅, 仲军, 陈琛, 羊彬, 胡道朋, 王余龙, 董桂春, 黄建晔. 氮素高效吸收型粳稻品种源库指标的基本特点. 扬州大学学报, 2012, 33(1): 54-60.
	Yu X F, Wang Y, Yuan Q M, Zhong J, Chen C, Yang B, Hu D P, Wang Y L, Dong G C, Huang J Y.Study on the characteristics of source and sink in conventionaljaponica rice cultivars with high nitrogen uptake efficiency. J Yangzhou Univ, 2012, 33(1): 54-60.

吸氮量、产量类型 Type	株系数 No. of lines	成熟期吸氮量 Nitrogen absorption per unit area/(g·m^-2)		产量 Grain yield /(kg·hm^-2)	株系分类 Classification of lines
吸氮量、产量类型 Type	株系数 No. of lines	成熟期吸氮量 Nitrogen absorption per unit area/(g·m^-2)		产量 Grain yield /(kg·hm^-2)	吸氮量类型 Type of nitrogen absorption	产量类型 Type of yield
低吸氮低产A	12		13.14±0.21 e	6 743.10±211.50 e	低Low		低Low
低吸氮中产B	17		14.37±0.11 d	7 585.35±64.20 d	低Low		中Medium
中吸氮中产C	40		15.98±0.10 c	8 211.00±87.15 c	中Medium		中Medium
中吸氮中产D	12		16.36±0.24 c	7 825.65±179.55 cd	中Medium		中Medium
中吸氮高产E	25		17.52±0.17 b	9 031.20±71.40 b	中Medium		高 High
高吸氮高产F	8		20.37±1.33 a	10 141.50±402.30 a	高High		高 High
F		51.439^**		49.55^**

吸氮量、产量类型 Type	株系数 No. of lines	成熟期吸氮量 Nitrogen absorption per unit area/(g·m^-2)		产量 Grain yield /(kg·hm^-2)	株系分类 Classification of lines
吸氮量、产量类型 Type	株系数 No. of lines	成熟期吸氮量 Nitrogen absorption per unit area/(g·m^-2)		产量 Grain yield /(kg·hm^-2)	吸氮量类型 Type of nitrogen absorption	产量类型 Type of yield
低吸氮低产A	12		13.14±0.21 e	6 743.10±211.50 e	低Low		低Low
低吸氮中产B	17		14.37±0.11 d	7 585.35±64.20 d	低Low		中Medium
中吸氮中产C	40		15.98±0.10 c	8 211.00±87.15 c	中Medium		中Medium
中吸氮中产D	12		16.36±0.24 c	7 825.65±179.55 cd	中Medium		中Medium
中吸氮高产E	25		17.52±0.17 b	9 031.20±71.40 b	中Medium		高 High
高吸氮高产F	8		20.37±1.33 a	10 141.50±402.30 a	高High		高 High
F		51.439^**		49.55^**

吸氮量、产量类型 Type	吸氮量每增加1 g所增加的产量 Yield increase per gram nitrogen absorption increment/g
A	/
B	54.91
C	95.02
D	68.95
E	148.16
F	219.33

吸氮量、产量类型 Type	吸氮量每增加1 g所增加的产量 Yield increase per gram nitrogen absorption increment/g
A	/
B	54.91
C	95.02
D	68.95
E	148.16
F	219.33

吸氮量、产量类型 Rice type	抽穗期有效叶面积系数 Effective LAI at heading stage	抽穗期高效叶面积系数 Efficient LAI at heading stage	抽穗期总叶面积系数 Total LAI at heading stage	成熟期叶面积系数 Efficient LAI at mature stage
A	5.04±0.22 b	3.08±0.11 b	5.42±0.24 b	1.76±0.14 c
B	5.32±0.16 b	3.21±0.09 b	5.67±0.18 b	1.99±0.09 bc
C	5.49±0.13 b	3.38±0.08 b	5.86±0.12 b	2.10±0.07 bc
D	5.34±0.22 b	3.28±0.17 b	5.70±0.21 b	2.22±0.10 bc
E	5.72±0.10 ab	3.50±0.07 ab	6.10±0.11 ab	2.44±0.11 b
F	6.52±0.50 a	4.11±0.35 a	6.87±0.56 a	3.48±0.53 a
r	0.423^**	0.462^**	0.433^**	0.686^**
F	3.609^**	3.963^**	3.210^**	7.918^**