中国水稻科学 ›› 2016, Vol. 30 ›› Issue (5): 458-468.DOI: 10.16819/j.1001-7216.2016.5180

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干旱胁迫下杂草稻和栽培稻根系基因表达差异研究

丁国华1,2, 孙健2, 杨光3, 张凤鸣1, 白良明1, 孙世臣1, 姜树坤1, 王彤彤1, 郑洪亮1, 夏天舒1, 沈希宏4, 马殿荣2,*(), 陈温福2,*()   

  1. 1 黑龙江省农业科学院博士后科研工作站/黑龙江省农业科学院 耕作栽培研究所/中国科学院 北方粳稻分子育种联合研究中心, 哈尔滨 155086
    2沈阳农业大学 水稻研究所, 沈阳110866
    3辽宁省经济作物研究所, 辽宁 辽阳 111000
    4中国水稻研究所, 杭州 310006
  • 收稿日期:2015-12-09 修回日期:2016-01-23 出版日期:2016-09-10 发布日期:2016-09-10
  • 通讯作者: 马殿荣,陈温福
  • 基金资助:
    黑龙江省农业科学院博士后工作站;哈尔滨市科技局项目(2014RFQYJ125);黑龙江省农业科学院创新工程项目(2014QN006);黑龙江省农业科学院博士人员科研启动金项目(201507-16);国家水稻产业技术体系项目(CARS-01-43);北方寒地粳稻资源研究与新品种选育项目(2014BAD01B03-02)

Global Genome Expression Analysis of Root Genes under Drought Stress in Weedy Rice and Up-land Rice

Guo-hua DING1,2, Jian SUN2, Guang YANG3, Feng-ming ZHANG1, Liang-ming BAI1, Shi-chen SUN1, Shu-kun JIANG1, Tong-tong WANG1, Hong-liang ZHENG1, Tian-shu XIA1, Xi-hong SHEN4, Dian-rong MA2,*(), Wen-fu CHEN2,*()   

  1. 1Postdoctoral Scientific Research Station of HAAS/Cultivation and Farming Research Institute of HASS/ Northern Japonica Rice Molecular Breeding Joint Research Center, Harbin 150086, China
    2Shenyang Agricultural University, Shenyang 110866, China
    3Institute of Economic Crop of Liaoning, Liaoyang 111000, China
    4China National Rice Research Institute,Hangzhou 310006,China
  • Received:2015-12-09 Revised:2016-01-23 Online:2016-09-10 Published:2016-09-10
  • Contact: Dian-rong MA, Wen-fu CHEN

摘要:

利用Affymetrix水稻表达谱芯片(GeneChip Rice Genome Array)研究抗旱杂草稻HEB07-2与巴西陆稻(IAPAR9)在PEG模拟干旱胁迫下根系基因表达变化。结果表明,杂草稻HEB07-2转录组对干旱信号的响应程度与方向均与巴西陆稻存在很大差异,HEB07-2以正向调控为主,而巴西陆稻以负调控为主。干旱胁迫下杂草稻HEB07-2与巴西陆稻分别有6878个和2923个基因表达,其中HEB07-2受干旱胁迫诱导上调的基因有4693个,下调的基因有2185个;而巴西陆稻则分别有983个和1940个。在差异基因表达的倍数上也是HEB07-2高于巴西陆稻。进一步的GO分析表明,在干旱胁迫下,HEB07-2和IAPAR9根系基因响应途径的差异表现为HEB07-2在钾离子转运(GO:0006813)、次生物质代谢(GO:0019748)、细胞生长(GO:0016049)、葡萄糖代谢(GO:0006006)、跨膜离子转运器活性(GO:0015075)、亚铁血红素结合体(GO:0020037)、氧化还原酶活性(GO:0016491)等方面基因显著上调,这与生理数据和表型数据一致。

关键词: 杂草稻, 根系, 表达谱芯片, 耐旱

Abstract:

The expression changes of root genes in drought-resistance weedy rice HEB07-2 (Oryza sativa f. spontanea) and up-land rice IAPAR9 (Oryza sativa) were analyzed under polyethylene glycol(PEG)-simulated with drought stress condition with Affymetrix GeneChip rice genome array. The results indicated that the extent and direction of transcriptome response of HEB07-2 and IAPAR9 to drought differed greatly. For HEB07-2, among 6878 expressed genes, 4693 were up-regulated and 2185 were down-regulated under drought condition. For IAPAR9, among 2923 expressed genes,983 were up-regulated and 1940 were down-regulated. Analysis of differentially expressed genes in HEB07-2 and IAPAR9 showed that the weedy rice HEB07-2 had a higher changing fold than the up-land rice IAPAR9. Gene ontology analysis revealed that genes of HEB07-2 in potassium ion transporting(GO: 0006813), secondary metabolite(GO: 0019748), cell growth(GO: 0016049), glucose metabolism(GO:0006006), transmembrane ion transporter activity(GO:0015075), ferroheme coalition(GO:0020037), oxidordeuctase activity(GO:0016491)were significantly up-regulated in comparing with IAPAR9. These were consistent with the physiological and phenotypic data.

Key words: weedy rice, root, gene expression profiling, drought resistance

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