Identification and Mapping of QTLs for Cold Tolerance at the Seedling Stage in Common Wild Rice(Oryza rufipogon)
ZHENG Jia-xing1,2; MA Zeng-feng1,3;SONG Jian-dong1,2; LIU Chi1,3;LI Yong-tao4;HUANG Da-hui1,3; WEI Shao-li1,2;ZHANG Yue-xiong1,3; MI Ke2; HUANG Jin-yan1;CHEN Miao1,2;MENG Jiao-rong2; LI Rong-bai1, 2, 3,*; CHEN Bao-shan2,*
1Guangxi Crop Improvement and Biotechnology Laboratory, Guangxi Academy of Agricultural Sciences, Nanning 530007, China; 2College of Agriculture/College of Life Science and Technology/Guangxi Key Laboratory of Subtropical Bioresource Conservation and Utilization, Guangxi University, Nanning 530005, China; 3Institute of Rice Research, Guangxi Academy of Agricultural Sciences, Nanning 530007, China; 4The 43rd Middle School of Nanning, Nanning 530200, China; *Corresponding authors, E-mail: firstname.lastname@example.org; email@example.com)
Two core accessions of common wild rice (DP15 and DP30) as donor parents and the cultivar 9311 as recipient parent were used to construct chromosome segment substitution lines for identification of QTLs for cold tolerance at the seedling stage and QTL effect analysis. A QTL mapping population from the most coldtolerant substitution line was constructed to map majoreffect QTLs with SSR markers. The two donor lines, DP15 and DP30, showed obvious difference in the number, location and effect of QTLs for cold tolerance. In the 230 BC4F2 substitution lines in which substituted chromosome segments covered the whole genome of the two donors, a total of 19 cold tolerance QTLs were detected, which distributed throughout all the 12 chromosomes, and clustered on chromosome 3 and chromosome 8. These QTLs were separated each other in different chromosome substitution lines. The substitution line with the smallest effect QTL for cold tolerance and the line with the largest effect QTL showed 8% and 74% of survival rate after cold treatment, respectively. A maineffect QTL, qSCT31, was mapped between SSR markers RM15031 and RM3400, near the centromere on the long arm of chromosome 3 with a distance of 18 cM to the closest linkage markers, RM15040 and RM1164.
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