中国水稻科学 ›› 2020, Vol. 34 ›› Issue (6): 499-511.DOI: 10.16819/j.1001-7216.2020.0408

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应用东乡野生稻回交重组自交系分析水稻耐低氮产量相关性状QTL

吴婷1, 李霞1, 黄得润2, 黄凤林3, 肖宇龙1,*(), 胡标林1,*()   

  1. 1 江西省农业科学院水稻研究所/水稻国家工程实验室(南昌),南昌 330200
    2 中国水稻研究所国家水稻改良中心/水稻生物学国家重点实验室, 杭州 310006
    3 湖南省水稻研究所农业农村部长江中下游籼稻遗传重点实验室,长沙410125
  • 收稿日期:2020-04-30 修回日期:2020-06-03 出版日期:2020-11-10 发布日期:2020-11-10
  • 通讯作者: 肖宇龙,胡标林
  • 基金资助:
    江西省自然科学基金资助项目(20171ACB21071);国家自然科学基金资助项目(31760378);农业农村部长江中下游籼稻遗传育种重点实验室开放课题资助项目(2018KLMA02);水稻生物学国家重点实验室开放课题(20200201)

QTL Analysis for Yield Traits Related to Low Nitrogen Tolerance Using Backcrossing Recombinant Inbred Lines Derived from Dongxiang Wild Rice (Oryza rufipogon Griff.)

Ting WU1, Xia LI1, Derun HUANG2, Fenglin HUANG3, Yulong XIAO1,*, Biaolin HU1,*   

  1. 1 Rice Research Institute, Jiangxi Academy of Agricultural Sciences / Rice National Engineering Laboratory(Nanchang), Nanchang 330200, China
    2 Hunan Rice Research Institute/ Key Laboratory of indica Rice Genetics and Breeding in the Middle and Lower Reaches of Yangtze River Valley, Ministry of Agriculture and Rural Affairs, Changsha 410125, China
    3 State Key Laboratory of Rice Biology/Chinese National Center for Rice Improvement,China National Rice Research Institute, Hangzhou 310006, China;
  • Received:2020-04-30 Revised:2020-06-03 Online:2020-11-10 Published:2020-11-10
  • Contact: Yulong XIAO, Biaolin HU

摘要: 目的 东乡野生稻低氮耐性强,是水稻耐低氮育种的重要资源。鉴定东乡野生稻耐低氮基因对研究耐低氮遗传机制、培育耐低氮水稻品种具有重要意义。方法 利用协青早B//东乡野生稻/协BBC1F12回交重组自交系及其遗传图谱,应用Windows QTL Cartographer 2.5分析施氮肥和不施氮肥下的株高和产量相关性状QTL。结果 共检测到57个控制株高和产量性状的QTL,分布于10条染色体上的33个区域,单个QTL表型贡献率为3.17%~63.40%,其中32个QTL的增效等位基因来自东乡野生稻。19个QTL在施氮和未施氮条件下均检测到,38个QTL仅在单一环境下检测到显著效应,表明不同施氮水平下水稻性状的遗传机制不同。结论 43个QTL分别聚集于7条染色体上的14个QTL簇,表明不同性状涉及到共同遗传机制,并可通过分子标记辅助选择方法进行耐低氮有利等位基因的聚合育种。

关键词: 耐低氮, 东乡野生稻, 产量性状, 数量性状基因座

Abstract:

【Objective】Dongxiang wildrice (Oryzarufipogon Griff.) has strong low nitrogen toleranceand is an important germplasm for low nitrogen tolerance improvement. Identification of genes responsible for low nitrogen tolerance in Dongxiang wild rice is of great importance to understand molecular mechanisms of low nitrogen tolerance and develop rice varieties with low nitrogen tolerance. 【Method】Quantitative trait loci (QTLs) for plant height and yield traits under low and normal nitrogen conditions wasidentified using backcrossing recombinant inbred lines (BC1F12) derived from an interspecific cross Xieqingzao B // Dongxiang wild rice/Xieqingzao B and its genetic linkage. 【Result】A total of 57 QTLs were detected in 33 regions on all chromosome, exceptchromosome 4 and 8. They explained individually 3.17%~63.40% phenotypic variation, and 32 QTLsof them had favorable alleles derived from Dongxiang wild rice.NineteenQTLs were simultaneously detected under both nitrogen treatments, and 38QTLs were only identified under single nitrogen treatment, suggesting variousgenetic mechanismsin rice growth and yield formation under low and normal nitrogen conditions. 【Conclusion】Fourteen QTLclusters, 43 QTLs included, scattered on sevenchromosomes, indicatingthe common genetic-physiological mechanisms behind different traits, and theQTL pyramiding for low nitrogen tolerance can be achieved by molecular marker-assisted selection.

Key words: low nitrogen tolerance, Dongxiang wild rice (Oryzarufipogon Griff.), yield trait, quantitative trait locus

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