中国水稻科学 ›› 2020, Vol. 34 ›› Issue (2): 135-142.DOI: 10.16819/j.1001-7216.2020.9086
曹志斌1, 李瑶2, 曾博虹1, 毛凌华1, 蔡耀辉1, 吴晓峰1,*, 袁林峰1,*
收稿日期:
2019-07-26
修回日期:
2019-10-17
出版日期:
2020-03-10
发布日期:
2020-03-10
通讯作者:
吴晓峰,袁林峰
作者简介:
#共同第一作者
基金资助:
Zhibin CAO1, Yao LI2, Bohong ZENG1, Linghua MAO1, Yaohui CAI1, Xiaofeng WU1,*, Linfeng YUAN1,*
Received:
2019-07-26
Revised:
2019-10-17
Online:
2020-03-10
Published:
2020-03-10
Contact:
Xiaofeng WU, Linfeng YUAN
About author:
#These authors contributed equally to this work
摘要:
【目的】本研究旨在定位一个稻米垩白粒率高温耐性QTL,为外观品质育种及解析垩白粒率高温耐性的遗传机制提供依据。【方法】以非洲栽培稻耐热品种IRGC102309(Oryza glaberrima Steud.)和籼稻品种R9311(O. sativa L. subsp. indica Kato.)为亲本构建的栽培稻种间染色体片段导入系CSIL05-23为材料构建次级分离群体,结合人工气候室模拟灌浆期高温胁迫处理,采用垩白粒率高温钝感值为评价指标,对非洲栽培稻垩白粒率高温耐性 QTL 进行检测。【结果】 在BC6F2分离群体,利用单标记分析,发现第5染色体上的SSR标记RM1200与垩白粒率耐热性状极显著正相关(P=0.0005)。进一步利用BC6F3和BC6F4分离群体,采用QTL Cartographer 2.5软件和复合区间作图法在水稻第5染色体上的SSR标记RM1200-RM5796区间重复检测到一个灌浆期垩白粒率耐热性QTL, 命名为qHTCGR5,分别解释11.4%和17.5%表型变异。根据BC6F4分离群体的纯合重组体表型分组,利用置换作图方法将目标QTL同样定位在SSR标记RM1200-RM5796之间,遗传图距为1.3 cM,物理图距约为333.4 kb。【结论】 控制垩白粒率耐热性的qHTCGR5是一个能够用于稻米外观品质育种的新QTL。
中图分类号:
曹志斌, 李瑶, 曾博虹, 毛凌华, 蔡耀辉, 吴晓峰, 袁林峰. 非洲栽培稻垩白粒率耐热性QTL的定位[J]. 中国水稻科学, 2020, 34(2): 135-142.
Zhibin CAO, Yao LI, Bohong ZENG, Linghua MAO, Yaohui CAI, Xiaofeng WU, Linfeng YUAN. QTL Mapping for Heat Tolerance of Chalky Grain Rate of Oryza glaberrima Steud.[J]. Chinese Journal OF Rice Science, 2020, 34(2): 135-142.
图1 CSIL05-23与R9311在田间及人工气候室高温胁迫处理下垩白粒率耐热性 **差异极显著(p<0.01), t检验。
Fig. 1. Comparison of heat tolerance of chalky grain rate of CSIL05-23 and R9311 in field and artificial climatic chambers. **Significant differences at 0.01 level, t-test.
世代 Generation | 供体亲本 Donor parent (IRGC102309) | 受体亲本 Recipient parent (R9311) | 群体Population | ||||
---|---|---|---|---|---|---|---|
平均 Mean | 范围 Range | 标准差 SD | 峰度 Kurtosis | 偏度 Skewness | |||
BC6F2(n=200) | 1.074 | 2.36 | 2.18 | 1.52-2.62 | 0.23 | 0.17 | 0.12 |
BC6F3(n=368) | 1.054 | 2.23 | 2.11 | 1.48-2.68 | 0.31 | 0.23 | 0.32 |
BC6F4(n=430) | 1.082 | 2.06 | 2.03 | 1.42-2.56 | 0.27 | 0.17 | 0.12 |
表1 亲本及BC6F2、BC6F3、BC6F4群体垩白粒率高温钝感值的表型变异
Table 1 Phenotypic variation of insensitive value of heat tolerance of chalky grain rate in BC6F2, BC6F3 and BC6F4 population and their parents.
世代 Generation | 供体亲本 Donor parent (IRGC102309) | 受体亲本 Recipient parent (R9311) | 群体Population | ||||
---|---|---|---|---|---|---|---|
平均 Mean | 范围 Range | 标准差 SD | 峰度 Kurtosis | 偏度 Skewness | |||
BC6F2(n=200) | 1.074 | 2.36 | 2.18 | 1.52-2.62 | 0.23 | 0.17 | 0.12 |
BC6F3(n=368) | 1.054 | 2.23 | 2.11 | 1.48-2.68 | 0.31 | 0.23 | 0.32 |
BC6F4(n=430) | 1.082 | 2.06 | 2.03 | 1.42-2.56 | 0.27 | 0.17 | 0.12 |
图2 垩白粒率耐热性QTL遗传连锁图和似然区间 左右箱线图分别表示来自BC6F3和BC6F4群体的1 LOD 和2 LOD 似然区间位置。
Fig. 2. Genetic linkage map and likelihood intervals for QTL associated with heat tolerance of chalky grain rate. The left and right bars and whiskers indicate 1 logarithm of the odds (LOD) and 2 LOD likelihood intervals from BC6F3 and BC6F4 populations, respectively.
性状 Character | 群体 Population | 区间 Interval | LOD | 表型方差 Phenotypic variance/% | 加性效应 Additive effect/% |
---|---|---|---|---|---|
垩白粒率高温钝感值IV | BC6F3 | RM1200-RM5796 | 6.3 | 11.3 | -5.9 |
BC6F4 | RM1200-RM5796 | 7.4 | 17.5 | -11.8 | |
高温胁迫下的垩白粒率X1 | BC6F3 | RM1200-RM5796 | 5.9 | 9.5 | -5.6 |
BC6F4 | RM1200-RM5796 | 6.9 | 16.7 | -12.5 | |
正常温度条件下的垩白粒率X2 | BC6F3 | RM1200-RM5796 | 6.2 | 12.6 | -5.7 |
BC6F4 | RM1200-RM5796 | 7.1 | 16.5 | -11.2 |
表2 BC6F3与BC6F4世代灌浆期高温胁迫后垩白粒率耐热性QTL分析
Table 2 QTL analysis of heat tolerance of chalky grain rate in BC6F3 and BC6F4 populations.
性状 Character | 群体 Population | 区间 Interval | LOD | 表型方差 Phenotypic variance/% | 加性效应 Additive effect/% |
---|---|---|---|---|---|
垩白粒率高温钝感值IV | BC6F3 | RM1200-RM5796 | 6.3 | 11.3 | -5.9 |
BC6F4 | RM1200-RM5796 | 7.4 | 17.5 | -11.8 | |
高温胁迫下的垩白粒率X1 | BC6F3 | RM1200-RM5796 | 5.9 | 9.5 | -5.6 |
BC6F4 | RM1200-RM5796 | 6.9 | 16.7 | -12.5 | |
正常温度条件下的垩白粒率X2 | BC6F3 | RM1200-RM5796 | 6.2 | 12.6 | -5.7 |
BC6F4 | RM1200-RM5796 | 7.1 | 16.5 | -11.2 |
图3 利用置换作图法定位qHTCGR5 由380个BC6F3单株的分析数据构建QTL区域的连锁图。BC6F4纯合重组体的后代在灌浆期模拟高温逆境处理后, 根据垩白粒率及钝感值(IV=X1/X2)调查结果将qHTCGR5定位在RM1200和RM5796之间, 根据基因型将90个重组体分为14组。每组的重组体数目及与R9311和染色体片段导入系CSIL05-23之间的垩白粒率高温钝感表型差异显著性在右边标出。“a”表示重组体与R9311的表型值在0.05水平无显著差异:“b”表示重组体与CSIL05-23的表型值在0.05水平无显著差异。
Fig. 3. Mapping of qHTCGR5 by a substitution mapping strategy. Linkage map of the QTLs region produced with 380 BC6F3 plants. The number of recombinants between adjacent markers is indicated under the linkage map. Progeny testing of BC6F4 homozygous recombinants delimited the qHTCGR5 locus to the region between markers RM1200 and RM5796. The 90 recombinants were grouped into 14 groups based on genotypes. The numbers of recombinants in each group and phenotypic difference of each group from the controls CSIL05-23 and R9311 for mean insensitive value of heat tolerance of chalky grain rate are shown on the right. An “a” following the phenotypic value indicates that the mean phenotypic value of recombinant was not significantly different from that of R9311 at P < 0.05;a “b”indicates that the mean phenotypic value of recombinant was not significantly different from that of CSIL05-23 at P < 0.05.
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