水稻细菌性条斑病抗性新品系的创制及其农艺性状分析

doi:10.16819/j.1001-7216.2023.230406

中国水稻科学 ›› 2023, Vol. 37 ›› Issue (6): 587-596.DOI: 10.16819/j.1001-7216.2023.230406

水稻细菌性条斑病抗性新品系的创制及其农艺性状分析

冯爱卿, 汪聪颖, 苏菁, 封金奇, 陈凯玲, 林晓鹏, 陈炳, 梁美玲, 杨健源, 朱小源, 陈深^*()

广东省农业科学院植物保护研究所/广东省植物保护新技术重点实验室, 广州 510640

收稿日期:2023-04-20 修回日期:2023-05-22 出版日期:2023-11-10 发布日期:2023-11-14
通讯作者: ^*email: 1642841084@qq.com; chens@gdppri.com
基金资助:
广东省自然科学基金面上项目(2021A1515012497);国家水稻产业技术体系资助项目(CARS-01-35);广东省水稻产业技术体系资助项目(2023KJ105);广东省农业科学院中青年学科带头人“金颖之光”培养计划项目(R2020PY-JG007);江苏省重点研发计划(现代农业)子项目(BE2020318-3);广东省农业科学院“十四五”新兴学科团队建设项目(202116TD);2023年农业农村厅乡村振兴战略专项(粤农农计(2023)13号)

Development and Agronomic Traits Analysis of New Rice Resistance Lines to Xanthomonas oryzae pv. oryzicola

FENG Aiqing, WANG Congying, SU Jing, FENG Jinqi, CHEN Kailing, LIN Xiaopeng, CHEN Bing, LIANG Meiling, YANG Jianyuan, ZHU Xiaoyuan, CHEN Shen^*()

Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences/ Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou 510640, China

Received:2023-04-20 Revised:2023-05-22 Online:2023-11-10 Published:2023-11-14
Contact: ^*email: 1642841084@qq.com; chens@gdppri.com

摘要/Abstract

摘要：

【目的】 创制水稻细菌性条斑病抗性新品系，为进一步培育抗细菌性条斑病水稻新品种提供核心种质材料。【方法】 通过多代回交、自交，结合分子标辅助选择技术和GSR40K芯片检测，以来自孟加拉国含抗细菌性条斑病Xo2基因的稻种资源BHADOIA 303为供体，以优良籼稻品种五山丝苗为受体，培育抗水稻细菌性条斑病株系，并分别通过田间成株期人工喷雾法和浸入法接种鉴定这些株系的抗病性，评价其农艺性状。【结果】 分子标记和GSR40K芯片检测表明最终获得稳定携带Xo2基因片段的10个新品系(BC₄F₅)；无论是喷雾法接种还是渗入法接种，导入抗病基因的株系和五山丝苗对细菌性条斑病的抗性均差异显著，10个测定株系均表现抗水稻细菌性条斑病，其平均病情指数为5.00~10.37，病斑长度为0.70~0.99 cm，其中以株系1~3抗性表现最好。这3个抗性较好的株系的农艺性状及产量性状指标有些与五山丝苗相似，其中株系1的千粒重、谷粒长、宽与五山丝苗相似，结实率优于五山丝苗；株系2每穗总粒数与五山丝苗相似，谷粒比五山丝苗长些、宽些，千粒重优于五山丝苗；株系3千粒重、结实率与五山丝苗相似，单株穗数优于五山丝苗。【结论】 获得水稻细菌性条斑病抗性显著增强且农艺性状较优良的3个株系，为细菌性条斑病抗病育种提供了抗性新材料，有利于加快水稻细菌性条斑病抗性分子育种进程。

关键词: 水稻细菌性条斑病, Xo2, 抗性改良, 分子标记辅助选择, 农艺性状

Abstract:

【Objective】 This study aims to develop new rice resistance lines to bacterial leaf streak (BLS) and provide core germplasm materials for BLS resistance breeding. 【Method】 BHADOIA303 harboring the Xo2 gene from Bangladesh was used as the BLS resistance donor, and an elite indica variety Wushansimiao was chosen as the recipient, to develop resistant lines by advanced backcrossing, molecular marker-assisted selection and GSR40K chip detection. The BLS resistance lines was evaluated by artificial inoculation with pathogen inoculum spraying and syringe infiltration in the field, and their agronomic traits were evaluated synchronously. 【Result】 Molecular markers and GSR40K microarray detection confirmed the presence of the target gene Xo2 in 10 new lines (BC₄F₅). Significant differences were observed in the BLS resistance between the introgression lines and Wushansimiao, no matter whether they were inoculated by spraying or infiltration. The average disease index ranged from 5.00 to 10.37, with an average lesion length of 0.70 to 0.99 cm. Lines 1-3 showed the best resistance. The agronomic and yield traits of the 3 lines with better resistance were comparable to those of Wushansimiao. The 1000-grain weight, grain length and width of line 1 were similar to those of Wushansimiao, and its seed setting rate was better than that of Wushansimiao. Number of total grains per panicle of Line 2 was similar to that of Wushansimiao with longer and wider grains, and a higher 1000-grain weight. The 1000-grain weight and seed setting rate of Line 3 were similar to those of Wushansimiao, and the number of panicles per plant was better than that of Wushansimiao. 【Conclusion】 Three Xo2 resistance lines with better agronomic traits and greatly enhanced resistance to BLS were obtained, which would provide resistance elite germplasm materials and accelerate the process of BLS resistance molecular breeding.

Key words: rice bacterial leaf streak, Xo2, resistance improvement, molecular marker-assisted selection, agronomic trait

冯爱卿, 汪聪颖, 苏菁, 封金奇, 陈凯玲, 林晓鹏, 陈炳, 梁美玲, 杨健源, 朱小源, 陈深. 水稻细菌性条斑病抗性新品系的创制及其农艺性状分析[J]. 中国水稻科学, 2023, 37(6): 587-596.

FENG Aiqing, WANG Congying, SU Jing, FENG Jinqi, CHEN Kailing, LIN Xiaopeng, CHEN Bing, LIANG Meiling, YANG Jianyuan, ZHU Xiaoyuan, CHEN Shen. Development and Agronomic Traits Analysis of New Rice Resistance Lines to Xanthomonas oryzae pv. oryzicola[J]. Chinese Journal OF Rice Science, 2023, 37(6): 587-596.

图/表 7

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标记名称Primer	引物序列(5′-3′) Sequence(5′-3′)	片段长度 Fragment length/bp
RM12941	F：TTATGCCATGTGGTCCAATCAGC R：ATTTGAACCATTTGGGCCTTGG	187
M6-1	F：GAATACCCCCATGCGACACCCTA R：CAAGCCGTCCCACGGTGAGAA	200

标记名称Primer	引物序列(5′-3′) Sequence(5′-3′)	片段长度 Fragment length/bp
RM12941	F：TTATGCCATGTGGTCCAATCAGC R：ATTTGAACCATTTGGGCCTTGG	187
M6-1	F：GAATACCCCCATGCGACACCCTA R：CAAGCCGTCCCACGGTGAGAA	200

品种名称 Variety	喷雾法接种Spray inoculation			浸入法接种Infiltration inoculation			抗性综合评价Comprehensive evaluation of resistance
品种名称 Variety	病情指数Disease index /%		病级最高级 The highest grade of disease	病斑长度 Length of lesion /cm	病级最高级 The highest grade of disease		抗性综合评价Comprehensive evaluation of resistance
五山丝苗Wushansimiao		70.74±2.31 aA	9	3.24±0.09 aA		9	HS
BHADOIA 303		1.85±0.32 eD	1	0.54±0.07 fE		1	R
株系1 Line 1		5.37±0.85 cdC	1	0.70±0.08 eD		1	R
株系2 Line 2		5.00±0.00 dC	1	0.72±0.05 eCD		1	R
株系3 Line 3		5.19±0.32 dC	1	0.83±0.09 cdeCD		3	R
株系5 Line 5		5.74±0.32 cdC	1	0.76±0.08 deCD		3	R
株系8 Line 8		10.37±0.85 bB	3	0.99±0.12 bB		3	R
株系9 Line 9		5.37±0.32 cdC	1	0.76±0.10 deCD		3	R
株系10 Line 10		6.67±1.11 cdC	3	0.87±0.03 cdBCD		3	R
株系11 Line 11		6.30±0.85 cdC	3	0.82±0.08 cdeCD		3	R
株系12 Line 12		6.11±0.56 cdC	1	0.79±0.07 cdeCD		3	R
株系13 Line 13		7.04±0.85 cC	3	0.89±0.04 bcBC		3	R

品种名称 Variety	喷雾法接种Spray inoculation			浸入法接种Infiltration inoculation			抗性综合评价Comprehensive evaluation of resistance
品种名称 Variety	病情指数Disease index /%		病级最高级 The highest grade of disease	病斑长度 Length of lesion /cm	病级最高级 The highest grade of disease		抗性综合评价Comprehensive evaluation of resistance
五山丝苗Wushansimiao		70.74±2.31 aA	9	3.24±0.09 aA		9	HS
BHADOIA 303		1.85±0.32 eD	1	0.54±0.07 fE		1	R
株系1 Line 1		5.37±0.85 cdC	1	0.70±0.08 eD		1	R
株系2 Line 2		5.00±0.00 dC	1	0.72±0.05 eCD		1	R
株系3 Line 3		5.19±0.32 dC	1	0.83±0.09 cdeCD		3	R
株系5 Line 5		5.74±0.32 cdC	1	0.76±0.08 deCD		3	R
株系8 Line 8		10.37±0.85 bB	3	0.99±0.12 bB		3	R
株系9 Line 9		5.37±0.32 cdC	1	0.76±0.10 deCD		3	R
株系10 Line 10		6.67±1.11 cdC	3	0.87±0.03 cdBCD		3	R
株系11 Line 11		6.30±0.85 cdC	3	0.82±0.08 cdeCD		3	R
株系12 Line 12		6.11±0.56 cdC	1	0.79±0.07 cdeCD		3	R
株系13 Line 13		7.04±0.85 cC	3	0.89±0.04 bcBC		3	R

材料名称 Material	株高 Plant height /cm	穗长 Panicle length/cm	单株穗数 Number of panicles per plant		粒长 Grain length / mm		粒宽 Grain width /mm
株系1 Line 1	115.81±0.87 aA	23.71±0.33 cB	15.42±0.88 cC		9.39±0.17 bB		2.61±0.02 bBC
株系2 Line 2	113.55±0.72 bA	25.29±0.27 aA	14.67±0.61 cC		10.14±0.13 aA		2.82±0.08 aA
株系3 Line 3	109.28±1.69 cB	22.85±0.27 dC	21.58±1.00 aA		9.07±0.05 cB		2.73±0.05 aAB
五山丝苗Wushansimiao	107.41±0.72 dB	24.77±0.34 bA	18.50±1.37 bB		9.33±0.06 bB		2.56±0.05 bC
材料名称 Material	每穗总粒数 Total grains per panicle	结实率 Seed setting rate/%		千粒重 1000-grain weight/g		小区产量 Plot yield/kg
株系1 Line 1	164.93±5.74 bB	89.28±1.40 aA		21.75±0.85 bB		2.48±0.05 bB
株系2 Line 2	210.18±12.78 aA	58.54±1.41 dC		24.82±0.98 aA		2.51±0.10 bB
株系3 Line 3	156.81±23.87 bB	72.84±5.45 bB		21.41±0.32 bB		2.60±0.16 bB
五山丝苗Wushansimiao	207.82±13.20 aA	65.50±1.46 cBC		21.35±0.83 bB		3.01±0.12 aA

水稻细菌性条斑病抗性新品系的创制及其农艺性状分析

Development and Agronomic Traits Analysis of New Rice Resistance Lines to Xanthomonas oryzae pv. oryzicola

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