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    Research Progress in the Function of Basic Leucine Zipper (bZIP) Protein Family in Rice
    HAN Cong, HE Yuchang, WU Lijuan, JIA Lili, WANG Lei, E Zhiguo
    Chinese Journal OF Rice Science    2023, 37 (4): 436-448.   DOI: 10.16819/j.1001-7216.2023.221018
    Abstract1742)   HTML65)    PDF (1407KB)(1700)       Save

    As a large family of transcriptional regulators, basic leucine zipper (bZIP) proteins are widespread in eukaryotes. The bZIP proteins characteristically harbor a bZIP domain composed of two closely adjacent structural features: a DNA-binding basic region and the leucine zipper region. Annotations to eighty-nine bZIP transcription factor-encoding genes are available in the japonica rice genome, 45 of which are identified. They are involved in regulating rice growth and development and responses to biotic and abiotic stress, including seed dormancy and germination, floral transition, and photomorphogenesis, and stress and hormone signaling pathway, etc.

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    Advances in the Molecular Mechanism of Rice Plant Type
    LAN Jinsong, ZHUANG Hui
    Chinese Journal OF Rice Science    2023, 37 (5): 449-458.   DOI: 10.16819/j.1001-7216.2023.221102
    Abstract606)   HTML71)    PDF (401KB)(976)       Save

    The plant type of rice is one of the major factors determining rice yield. The ideotype of high-yielding rice cultivar is an important way to increase rice yield potential in rice breeding. Dwarf breeding in the 1950s and hybrid rice breeding in the 1970s are two revolutions in rice yield. However, the growth rate of rice yield has slowed down significantly in recent years. The third yield breakthrough depends on the super-high-yield breeding that combines ideotype with heterosis. We briefly reviewed the advances in the regulation mechanism of rice plant architecture, focusing on the progress achieved in leaf, panicle, grain and other aspects, and looked forward to the future research trends rice ideotype. It will lay a solid foundation for improving rice plant type and further increasing rice yield through molecular breeding.

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    Research Progress in Mechanism Behind Heat Damage and Its Regulatory Techniques During Flowering in Rice
    XU Yongqiang, JIANG Ning, FENG Baohua, XIAO Jingjing, TAO Longxing, FU Guanfu
    Chinese Journal OF Rice Science    2024, 38 (2): 111-126.   DOI: 10.16819/j.1001-7216.2024.230601
    Abstract602)   HTML54)    PDF (498KB)(855)       Save

    In recent years, extremely high temperatures have significantly impacted rice growth, yield, and quality. The flowering stage of rice is particularly sensitive to high temperatures, making it crucial to study their effects on spikelet fertility and cultivation techniques. To mitigate high-temperature damage to rice and enhance food security, this paper reviews the characteristics and mechanisms of rice floret opening, anther dehiscence, pollen scattering, pollen germination, and pollen tube elongation under high-temperature stress. It clarifies the regulatory mechanisms of heat tolerance during rice flowering and proposes cultivation measures to alleviate high-temperature damage. This paper offers new insights into heat resistance mechanisms and cultivation technologies at the rice flowering stage, with promising implications for future research.

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    Directed Knockout of SD1 Gene Improves Lodging Resistance and Blast Resistance of Rice
    LI Gang, GAO Qingsong, LI Wei, ZHANG Wenxia, WANG Jian, CHEN Baoshan, WANG Di, GAO Hao, XU Weijun, CHEN Hongqi, JI Jianhui
    Chinese Journal OF Rice Science    2023, 37 (4): 359-367.   DOI: 10.16819/j.1001-7216.2023.221113
    Abstract523)   HTML35)    PDF (1390KB)(863)       Save

    【Objective】 Huai 119 is a high-yielding japonica rice variety. To improve its unfavorable traits, especially high plant height and poor resistance to rice blast, we used the CRISPR/Cas9 gene editing technology to knock out its ‘Green Revolution’ gene SD1.【Method】 We selected the SD1 gene as the target to construct the CRISPR/Cas9 gene knockout vector, which was then transformed into Huai 119 by Agrobacterium-mediated transformation method, and the homozygous sd1 knockout line without transgenic insertion was obtained. Subsequently, we compared and analyzed the plant height, rice blast resistance, and nitrogen uptake and utilization efficiency of the sd1 and wild-type lines.【Result】 We successfully isolated a homozygous sd1 knockout line without transgenic insertion in the background of Huai 119. In field paddy, it was found that over 60% of the planting area of wild-type Huai 119 was lodging, while the sd1 homozygous mutant population effectively avoided lodging in the later stages of growth due to its shorter plant height. In addition, after treatment with different concentrations of GA (0.01-1.00 μmol/L), the plant height increase of wild-type plants was significantly greater than that of the sd1 line, indicating that the sensitivity of sd1 line to exogenous GA treatment was reduced. The identification of rice blast resistance shows that knocking out SD1 also contributed to the improvement of rice blast resistance of Huai 119. However, the absorption and utilization efficiency of nitrogen decreased due to the knockout of SD1. 【Conclusion】 The knockout of SD1 gene in the rice variety Huai 119 not only improves lodging resistance by reducing plant height, but also enhances the resistance to rice blast.

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    Prospects for the Application of Gene Editing and Genomic Selection in Rice Breeding
    LIANG Chuyan, WU Mingming, HUANG Fengming, ZHAI Rongrong, YE Jing, ZHU Guofu, YU Faming, ZHANG Xiaoming, YE Shenghai
    Chinese Journal OF Rice Science    2024, 38 (1): 1-12.   DOI: 10.16819/j.1001-7216.2024.230503
    Abstract498)   HTML48)    PDF (324KB)(962)       Save

    Rice is the main grain crop in China. Due to large population and limited land resources in China, rice breeding goals have long been yield oriented. Chinese researchers have continuously achieved breakthroughs in rice breeding technology, and the adoption of dwarf breeding and hybrid rice breeding technology has resulted in two significant leaps in rice yield in China. However, with the improvement of living standards and the increasing frequency of extreme weather events, higher demands have been placed on rice in terms of yield, quality, and resistance. At present, biotechnology is undergoing constant innovation, particularly with the rapid development of gene editing and genomic selection breeding technology. This progress is expected to provide robust support for the development of new rice varieties with high yield, superior quality, and resistance to multiple factors. It helps promote the green and sustainable development of rice production. This paper reviews the recent advancements in gene editing technology and genomic selection technology in rice breeding for high yield, superior quality, disease and insect resistance, stress tolerance, and heterosis. The goal is to provide breeding strategies for the efficient development of new varieties to meet demands.

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    Research Progress in Mining and Utilization of Rice Blast Resistance Genes
    YANG Jie, YANG Changdeng, ZENG Yuxiang, HOU Yuxuan, CHEN Tianxiao, LIANG Yan
    Chinese Journal OF Rice Science    2024, 38 (6): 591-603.   DOI: 10.16819/j.1001-7216.2024.230911
    Abstract479)   HTML69)    PDF (430KB)(1309)       Save

    Rice blast is a disease caused by Magnaporthe oryzae, which seriously threatens the yield and quality of rice. At present, breeding disease-resistant varieties is the most economical and effective way to control rice blast, and identifying resistance genes is the key to disease-resistant variety breeding. The review summarized the types of rice blast resistance genes cloned as well as the current mainstream techniques for identifying blast-resistant genes. The application of these genes has been outlined along with future development directions.

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    Research Progress in Biological Functions of SPL Family Transcription Factors in Rice
    HU Li, YANG Fanmin, CHEN Weilan, YUAN Hua
    Chinese Journal OF Rice Science    2024, 38 (3): 223-232.   DOI: 10.16819/j.1001-7216.2024.230308
    Abstract452)   HTML39)    PDF (1349KB)(873)       Save

    SPL (Squamosa Promoter-binding protein-like) family proteins are plant specific multifunctional transcription factors. There are 19 OsSPL genes in rice, all of which contain a highly conserved SBP domain, which is responsible for binding to the core motif GTAC of the downstream target gene to regulate the target gene expression. The expression of OsSPL is regulated by OsmiR156/529/535 and various factors. Studies have shown that OsSPL plays important roles in many biological processes of rice, including root development, ligule and auricles development, the formation of plant architecture and panicle morphology, grain development and stress response, and is a regulatory hub for rice growth and development. In this review, we summarized the research progress of the OsSPL family in rice, including its phylogenetic evolution, structural characteristics, expression regulation and biological functions, and the research prospects were also discussed.

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    Development of Aromatic Salt-tolerant Rice Based on CRISPR/Cas9 Technology
    LI Jingfang, WEN Shuyue, ZHAO Lijun, CHEN Tingmu, ZHOU Zhenling, SUN Zhiguang, LIU Yan, CHEN Haiyuan, ZHANG Yunhui, CHI Ming, XING Yungao, XU Bo, XU Dayong, WANG Baoxiang
    Chinese Journal OF Rice Science    2023, 37 (5): 478-485.   DOI: 10.16819/j.1001-7216.2023.220907
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    【Objective】 It is of importance to promote salt-tolerant and fragrant rice breeding. We obtained transgene free homozygous rice materials with salt-tolerance and fragrance by CRISPR/Cas9-mediated editing of Badh2 and OsRR22 in japonica variety Lianjing 11. 【Method】 Target sites were designed according to knockout efficiency of editing sites in Badh2 and OsRR22 gene sequence. The pH-Ubi-Badh2-OsRR22 knockout vector was constructed and transferred into the recipient variety Lianjing 11 by the Agrobacterium-mediated technology. PCR detection for hygromycin, Cas9 marker and target gene sequencing were performed in the transgenic progenies to obtain badh2-osrr22 homozygous lines without exogenous gene insertion. The seed traits and salt tolerance at seedling stage were analyzed. 【Result】 Compared with the background material Lianjing 11, the 2-AP content of T2 significantly increased. After treated with 128 mmol/L NaCl solution for 14 days, the seedling height, the fresh and the dry weight of T2 line 21-30 increased by 15.2%, 45.2% and 13.2%, respectively. 【Conclusion】 The successful editing of Badh2 and OsRR22 by CRISPR/Cas9 technology developed aromatic salt-tolerant homozygous lines without exogenous gene insertion, speeding up the breeding process of pyramiding multiple traits in rice.

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    Research Progress on the Damage and Management of Apple Snails(Ampullariider: Pomacea) in Paddy Fields
    LÜ Haitao, LI Jianzhong, LU Yanhui, XU Hongxing, ZHENG Xusong, LÜ Zhongxian
    Chinese Journal OF Rice Science    2024, 38 (2): 127-139.   DOI: 10.16819/j.1001-7216.2024.230807
    Abstract441)   HTML29)    PDF (379KB)(895)       Save

    Rice is a widely cultivated food crop in China and around the world, with over half of the global population depending on it as their primary food source. The golden apple snail, Pomacea canaliculata, as an invasive species, poses a significant threat to rice production, garnering extensive attention in China. From sowing to field sundrying, rice is susceptible to being bitten by P. canaliculata. During sowing, it bites the embryo of rice seeds, and at the seedling stage, it attacks the entire seedling, leading to a reduction in rice seedlings and effective tillers, significantly impacting rice yield. This paper reviews the species, biological characteristics, damage patterns in paddy fields, field prevention and control technology, and resource utilization of apple snails. The aim is to lay a foundation for further prevention and control measures in paddy fields, ultimately increasing rice yield and quality and minimizing economic losses for farmers.

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    Research Progress in Physiological,Biochemical Responses of Rice to Drought Stress and Its Molecular Regulation
    GUO Zhan, ZHANG Yunbo
    Chinese Journal OF Rice Science    2024, 38 (4): 335-349.   DOI: 10.16819/j.1001-7216.2024.230410
    Abstract420)   HTML47)    PDF (452KB)(1304)       Save

    Rice is one of the most important food crops globally, and its growth requires more water than many other crops. With global warming, drought has emerged as the main factor limiting crop yields. Therefore, based on recent research achievements, this paper summarizes the self-protection mechanisms of rice under drought stress. This includes aspects such as morphology (plant height, roots, leaves, number of tillers, and plant biomass), physiology (stomata, transpiration, photosynthesis, and water use efficiency), biochemistry (plant hormones, proline, other osmotic regulators, and antioxidants), and molecular biology (expression levels of drought resistance genes). The aim is to comprehensively understand rice's drought resistance mechanisms and provide a reference for breeding drought-resistant varieties

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    Sterility Mechanism of Photoperiod/Thermo-sensitive Genic Male Sterile Lines and Development and Prospects of Two-line Hybrid Rice
    WANG Yichen, ZHU Benshun, ZHOU Lei, ZHU Jun, YANG Zhongnan
    Chinese Journal OF Rice Science    2024, 38 (5): 463-474.   DOI: 10.16819/j.1001-7216.2024.231008
    Abstract420)   HTML25)    PDF (1010KB)(951)       Save

    This review documented the research progress of two-line hybrid rice in China, and introduced germplasm resources of photoperiod/thermo-sensitive genic male sterile (P/TGMS) lines in two-line hybrid rice breeding. We also summarized various genetic and cellular mechanisms of P/TGMS in plants, and suggestions were proposed for the genetic resource optimization and breeding strategies of P/TGMS lines in two-line hybrid rice breeding.

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    Research Progress on Correlation of Rice Leaf Senescence and Discoloration with Nitrogen Reuse and Volatilization
    TAO Yi, XU Yanan, YE Chang, ZHENG Guangjie, XU Chunmei, CHEN Song, CHU Guang, LIU Yuanhui, WANG Danying
    Chinese Journal OF Rice Science    2023, 37 (6): 553-562.   DOI: 10.16819/j.1001-7216.2023.230605
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    Leaf senescence is a critical development stage in the plant lifecycle, and leaf discoloration is a typical marker. The investigation on N recycle and reuse during the leaf color changing process can provide insights into the influence factors of N remobilization efficiency. This review delivers a brief overview about the internal and external factors that influence rice leaf discoloration and the types of stay-green varieties. Moreover, we summarized the latest research progress on the association between nitrogen reutilization-volatilization and rice leaf discoloration. This review elucidated the reasons behind the increasing nitrogen volatilization during the late reproductive stage of rice, and made a strong case for the development of straightforward leaf discoloration monitoring technology to create a quantitative indicator system for leaf discoloration characteristics. Such a review would clarify the relationships and mechanisms between rice leaf senescence dynamics and nitrogen reutilization and volatilization, photosynthetic assimilate accumulation, and nitrogen activation reutilization in the maturation process.

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    Effects of Nitrogen Fertilizer Consumption and Operation on Rice Yield and Its Components in China:A Meta-analysis
    XIAO Dakang, HU Ren, HAN Tianfu, ZHANG Weifeng, HOU Jun, REN Keyu
    Chinese Journal OF Rice Science    2023, 37 (5): 529-542.   DOI: 10.16819/j.1001-7216.2023.221111
    Abstract393)   HTML49)    PDF (2425KB)(806)       Save

    【Objective】 Moderate nitrogen (N) fertilizer rate and operation can effectively improve rice yield and N use efficiency. Clarifying the effects of N rate fertilizer rate and operation on rice yield and its components can provide theoretical guidance for high yield and high efficiency for rice. 【Methods】 Based on 119 published articles on N fertilizer management in rice fields, a meta-analysis was conducted to summarize the effects of N management on rice yield and its components under different total N rates, basal fertilizer+tillering fertilizer, topdressing for panicle initiation, planting areas and soil properties, and explored the appropriate measures to improve yield components to get high yield in major Chinese rice production areas. 【Results】 Compared with no N fertilizer application, N fertilizer application can significantly increase the actual yield (+42.2%) and theoretical yield (+43.1%), effective panicle number and grain number per panicle increased by 33.2% and 13.5%, respectively, while the seed setting rate and 1000-grain weight decreased by 4.2% and 1.6%, respectively. There were significant differences in rice yield and its components under different N rates and N operations. The actual and theoretical yield of rice increased significantly at the N rate of 150-200 kg/hm2, while the effective panicle number and grain number per panicle increased most at the N rate of 250-300 kg/hm2. In addition, with the increase of N rates, the seed setting rate and 1000-grain weight decreased significantly. When the ratio of basal fertilizer+tillering fertilizer to the total nitrogen application was 30%-50% and the N ratio of panicle fertilizer was 10%-30%, the rice yield peaked, while increasing the ratio of basal fertilizer+tillering fertilizer (≤70%) can significantly increase effective panicle number, but reduce grain number per panicle and seed setting rate. And, increasing the ratio of topdressing for panicle initiation (≤30%) can increase grain number per panicle and seed setting rate. For different rice growing regions, there were significant differences for rice yield and its components. The main performance is that the yield increase in the single-season rice from Northeast China is the largest, followed by the single-season and double-season rice in the Yangtze River basin, and the single-season and double-season rice region in South China and the single-season rice in the Yunnan-Guizhou and Sichuan-Hunan Plateau are the smallest. All rice regions achieved high yield by increasing the number of effective panicles and grain number per panicle. The increase of actual and theoretical yield of rice was less affected by SOM(soil organic matter), and the difference between the increase of each SOM level was not more than 4.39% and 2.26%, while the changes of soil TN(total nitrogen), AN(available nitrogen), AP(available phosphorus), AK(available potassium) contents had a greater impact, but there was no significant difference between subgroups. 【Conclusion】 The recommended N rate should not exceed 250 kg/hm2, and the recommended ratio of base fertilizer +tillering fertilizer should not exceed 70% of the total N rate. When the ratio of panicle fertilizer is 10%-30%, it is more beneficial to increase rice yield. Under the condition of ensuring the effective panicles and grain number per panicle of rice, improving the seed setting rate is the key to increase yield in all the rice planting regions in China, which needs comprehensive management measures such as chemical regulation, cultivation density and excellent varieties.

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    Research Progress on the Relationship Between Rice Root, Soil Properties and Methane Emissions in Paddy Fields
    CHEN Haotian, QIN Yuan, ZHONG Xiaohan, LIN Chenyu, QIN Jinghang, YANG Jianchang, ZHANG Weiyang
    Chinese Journal OF Rice Science    2024, 38 (3): 233-245.   DOI: 10.16819/j.1001-7216.2024.231206
    Abstract392)   HTML36)    PDF (1151KB)(1134)       Save

    The article highlights the critical role of methane (CH4) emissions from rice paddies in contributing to global climate warming and underscores the importance of studying the relationship between rice roots, soil characteristics, and CH4 emissions to mitigate the greenhouse effect. It reviews the mechanisms driving CH4 production and emission in rice paddies, emphasizing the significance of rice roots and soil conditions in influencing these emissions. Additionally, the paper suggests avenues for further research to deepen our understanding of the interplay between rice, soil characteristics, and CH4 emissions from paddy fields. By elucidating these relationships, the study aims to provide a theoretical framework for achieving both high grain yields and carbon sequestration in paddy field ecosystems.

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    OsOPR10 Positively Regulates Rice Blast and Bacterial Blight Resistance
    XU Danjie, LIN Qiaoxia, LI Zhengkang, ZHUANG Xiaoqian, LING Yu, LAI Meiling, CHEN Xiaoting, LU Guodong
    Chinese Journal OF Rice Science    2024, 38 (4): 364-374.   DOI: 10.16819/j.1001-7216.2024.231215
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    【Objective】 Jasmonic acid (JA) plays crucial roles in plant growth and development, and responses to both biotic and abiotic stresses. OPR (12-oxo-phytodienoic acid reductase) serves as a pivotal enzyme in the JA biosynthetic pathway. In this study, transgenic rice plants overexpressing OsOPR10 were generated to assess their resistance to rice blast and bacterial blight. The research delved into the molecular mechanisms through which OsOPR10 regulates the defense response to these diseases.【Method】 Methodologically, the study involved the construction of OsOPR10 CRISPR/Cas9 knockout and overexpression vectors. These vectors were then utilized for Agrobacterium-mediated genetic transformation to obtain OsOPR10 knockout (OsOPR10-KO) and overexpressed (OsOPR10-OE) transgenic rice plants, using Nipponbare (NPB) as the wild-type parent. The transgenic plants underwent various assays to evaluate disease resistance, reactive oxygen species (ROS) burst, and the expression of genes related to the salicylic acid (SA) and jasmonic acid (JA) pathways. Additionally, the subcellular localization of OsOPR10 was examined using a laser confocal microscopy, and the interacting protein of OsOPR10 was identified through yeast two-hybrid screening and luciferase complementation experiments.【Result】 The results of the study indicated the successful generation of homozygous plants with OsOPR10 knockout and overexpression. Plants overexpressing OsOPR10 exhibited enhanced resistance to rice blast and bacterial blight. Upon induction with chitin and bacterial flagellin (flg22), ROS accumulation in OsOPR10-OE plants was notably higher than that in the wild type. Furthermore, qRT-PCR analysis revealed up-regulation of JA pathway genes (OsAOS2, OsAOC) and SA pathway genes (OsPR1a, OsPAL1) in OsOPR10-OE plants compared to NPB at 12 hours post-inoculation with Magnaporthe oryzae. Subcellular localization studies demonstrated that the OsOPR10 protein was localized in chloroplasts. The interaction protein OsCYP28 of OsOPR10 was identified through yeast two-hybrid assays and luciferase complementation experiments 【Conclusion】 In conclusion, OsOPR10 plays a significant role in responding to infections by Magnaporthe oryzae and Xanthomonas oryzae pv. oryzea, as well as to the application of exogenous methyl jasmonate (MeJA) and SA. OsOPR10 is involved in the pathogen molecular pattern-triggered immune pathway and positively regulates rice resistance to rice blast and bacterial blight through the JA and SA pathways. Additionally, OsOPR10 protein localizes in chloroplasts and interacts with the OsCYP28 protein.

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    Effect of Plant-row Spacing on Rice Yield and Root Morphological and Physiological Characteristics in Super High Yield Field
    DONG Liqiang, YANG Tiexin, LI Rui, SHANG Wenqi, MA Liang, LI Yuedong, SUI Guomin
    Chinese Journal OF Rice Science    2023, 37 (4): 392-404.   DOI: 10.16819/j.1001-7216.2023.221007
    Abstract355)   HTML27)    PDF (8622KB)(744)       Save

    【Objective】 It is very crucial to investigate the effects of plant-row spacing on rice yield in super-high-yield field and clarify the mechanism behind high yield of rice in high-density mode.【Method】 In 2020 and 2021, Liaojing 419 was grown under five cultivation modes including local farmers' mode (LFM), conventional high-density mode (CHMD), narrow-row high-density mode (NHDM) and two wide and narrow row modes (WNHDM1 and WNHDM2). We measured the root morphological and physiological indexes and the composition factors of rice yield to explore the effects of plant and row spacing on rice yield and root morphological and physiological characteristics in super high-yield fields, clarify the relationship between root distribution and yield composition factors of rice in high-density mode, and reveal the mechanism of high yield of rice in super-high-yield fields in high-density mode.【Result】 Compared with LFM, the productive panicle number under WNHDM1 and WNHDM2 increased by 19.24% and 18.16%, respectively, and grain number per panicle and seed setting rate remained stable. The root indexes of IN(narrow row) area in WNHDM1 and WNHDM2 were not lower than those of other modes, but the difference was greater in OUT(wide row) area, total root length, total root diameter, total root surface area, total root volume and total root dry weight increased by 21.07% and 26.76%, 10.71% and 9.18%, 21.13% and 26.77%, 21.15% and 27.62% and 23.48% and 29.71% in two growing seasons. The root morphological indices in WNHDM1 and WNHDM2 had a positive correlation with panicle number and yield at heading stage and filling stage.【Conclusion】 The high-density mode with optimized plant and row spacing provides rice with asymmetric growth space inside and outside the row, exerts root marginal effect, improves root dry weight, root surface area and root volume, maintains strong root oxidation activity, lays a good basis for the formation of more productive panicles, and achieves the purpose of increasing rice yield.

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    Map-based Cloning and Functional Analysis of a New Allele of D1, a Gene Controlling Plant Height in Rice (Oryza sativa L.)
    GAO Junru, QUAN Hongyu, YUAN Liuzhen, LI Qinying, QIAO Lei, LI Wenqiang
    Chinese Journal OF Rice Science    2024, 38 (2): 140-149.   DOI: 10.16819/j.1001-7216.2024.230502
    Abstract351)   HTML33)    PDF (5304KB)(584)       Save

    【Objective】Plant height is an important agronomic trait in rice breeding. Identification of genes controlling plant height and their functional characterization can provide useful genetic resources for high-yield breeding.【Methods】A dwarf mutant, d1-11, was screened from the rice variety Nipponbare by EMS mutagenesis. Phenotypic and cytological observations, gene expression, hormone content and drought resistance were analyzed. The d1-11 mutant gene was identified through map-based cloning.【Results】The d1-11 mutant exhibits dwarfism, with more shortened and widened leaves, and more rounded grains compared to the wild type. The d1-11 mutant leaf has a smaller midvein, reduced number and area of large and small veins, resulting in abnormal leaf morphology in the d1-11 mutant. The d1-11 gene is genetically mapped between two molecular markers on rice chromosome 5. Map-based cloning reveals that a single base substitution at the junction of the ninth exon-intron in the D1 gene results in the loss-of-function mutation of d1-11. The D1 gene has a higher expression level in various tissues at the seedling stage, but the expression levels decrease from the tillering stage. Exogenous abscisic acid (ABA) treatment for 24 hours induces D1 gene expression, exogenous gibberellin (GA) treatment inhibits D1 gene expression, and salt stress treatment for 24 hours can strongly induce D1 gene expression. The contents of several hormones such as GA, brassinosteroid (BR), and indole-3-acetic acid (IAA) were increased in the d1-11 mutant. The d1-11 mutant shows a significant increase in relative water content (RWC) and a reduced rate of water loss in leaves. Furthermore, d1-11 mutant plants exhibit stronger resistance to drought stress.【Conclusion】The d1-11, a novel allele in D1 locus was identified in the present study. It was showed that the d1-11 mutant had increased levels of various endogenous hormones, increased leaf water content, and enhanced resistance to drought stress. This study will further enrich the genetic resources related to dwarfism and reveals some new biological roles of the D1 gene in rice.

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    Research Progress in the Effect of Salinity, Drought, and Their Combined Stresses on Rice Yield and Quality Formation
    WEI Huanhe, MA Weiyi, ZUO Boyuan, WANG Lulu, ZHU Wang, GENG Xiaoyu, ZHANG Xiang, MENG Tianyao, CHEN Yinglong, GAO Pinglei, XU Ke, HUO Zhongyang, DAI Qigen
    Chinese Journal OF Rice Science    2024, 38 (4): 350-363.   DOI: 10.16819/j.1001-7216.2024.240205
    Abstract350)   HTML31)    PDF (603KB)(596)       Save

    The production of rice in coastal saline-alkali lands is constrained by limited freshwater resources and water infrastructure. The presence of salinity damage and drought, often occurring together, renders rice production in these areas highly susceptible to combined salinity-drought stress, significantly impeding the attainment of high yield and quality in rice in coastal saline-alkali lands. A comprehensive analysis of the impacts of salinity damage, drought, and their combined stresses on rice yield and quality formation, along with their physiological mechanisms, can offer scientific backing for optimizing high-yield and quality rice cultivation in coastal saline-alkali lands. This article presents an overview of the effects of salinity damage, drought, and their combined stresses on the growth, development, yield formation, and grain quality of rice. The mechanisms underlying their influence on rice yield and quality formation are elucidated, focusing on osmotic regulation, ion balance, photosynthetic functions, antioxidant enzyme systems, endogenous hormones, key enzyme activities related to sucrose-starch metabolism, and molecular mechanisms. Recommendations are provided for the selection of salinity- and drought-tolerant varieties, as well as cultivation practices to mitigate salinity and drought stress in rice. Lastly, suggestions are proposed for further research on salinity and drought stress in rice.

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    Identification of Candidate Genes for Rice Nitrogen Use Efficiency by Genome-wide Association Analysis
    LÜ Yang, LIU Congcong, YANG Longbo, CAO Xinglan, WANG Yueying, TONG Yi, Mohamed Hazman, QIAN Qian, SHANG Lianguang, GUO Longbiao
    Chinese Journal OF Rice Science    2024, 38 (5): 516-524.   DOI: 10.16819/j.1001-7216.2024.231010
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    Objective】 The exploration of germplasm and gene resources in rice for high nitrogen efficiency, along with the elucidation of their molecular mechanisms and genetic effects, represents a significant focus and goal within current research efforts on rice nitrogen use efficiency (NUE).【Method】 To identify the variant loci and candidate genes associated with rice NUE, we collected 190 Asian rice accessions as an association population. After thorough filtering and screening, we obtained 3,934,195 high-quality single nucleotide polymorphisms (SNPs). Under field conditions, two nitrogen treatment levels were established: low nitrogen (N1, 90 kg/hm²) and normal nitrogen (N2, 180 kg/hm²). We investigated the phenotypic data of rice leaf width in response to both low and normal nitrogen treatments at the maturity stage. Genome-wide association analysis (GWAS) was conducted by integrating the FarmCPU and MLM models. 【Result】By calculating the phenotypic ratio Q (N1/N2) of leaf width under low and normal nitrogen levels, we found that the Q value exhibited a normal distribution. A total of 100 significant loci were identified on 12 chromosomes through GWAS for the Q value, leading to the determination of 39 candidate QTLs. This included the cloned NUE-related genes OsNR1.2 and OsNAC42. Additionally, we identified superior haplotypes and potential advantageous haplotype combinations of the candidate genes OsNR1.2 and OsNAC42, which provide valuable resources and information for enhancing rice NUE. 【Conclusion】This study elucidated the genetic basis of rice leaf width under varying nitrogen treatments using GWAS and haplotype analysis. We identified candidate QTLs and genes associated with NUE, including OsNR1.2 and OsNAC42. Through haplotype analysis, we recognized advantageous haplotype combinations of these two genes, offering valuable resources and insights for the improvement of rice NUE.

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    Mechanisms Behind Aleurone Development in Cereals and Its Application in Breeding
    WANG Tengjiao, CHEN Chen
    Chinese Journal OF Rice Science    2023, 37 (5): 459-469.   DOI: 10.16819/j.1001-7216.2023.230105
    Abstract342)   HTML20)    PDF (4022KB)(733)       Save

    The outmost layer of endosperm cells differentiates into alleurone cells during seed development, which distinguish themselves from the inner endosperm cells morphologically and physiologically. For example, the aleurone layer is rich in proteins, lipids, vitamins, dietary fibers and mineral elements. The aleurone cells secrete amylase and protease for hydrolyzing reserves accumulated in the endosperm. Several key genes involved in the regulation of aleurone cell differentiation and development have been identified with the mutants showing aleurone defects, deepening our understanding of the aleurone cell fate determination. It has been proposed that increasing the number of aleurone layers may help improve nutritional quality of cereals. In this review, taking rice as an example, we summarized the differentiation and development regulations of aleurone in cereals. We also discussed the potentials, issues and possible solutions for breeding high-nutrition cereals by improving the aleurone-related traits.

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