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    Recent Advances in Mechanisms of Adaptation of Planthoppers to Rice Resistance
    WANG Yaxuan, WANG Xinfeng, YANG Houhong, LIU Fang, XIAO Jing, CAI Yubiao, WEI Qi, FU Qiang, WAN Pinjun
    Chinese Journal OF Rice Science    2025, 39 (3): 306-321.   DOI: 10.16819/j.1001-7216.2025.240812
    Abstract800)   HTML19)    PDF (1373KB)(857)       Save

    The rice planthoppers, including the brown planthopper, white-backed planthopper, and small brown planthopper, are major threats to global rice production. To combat these pests, rice has evolved defense mechanisms, including antibiosis, antixenosis or non-preference, and tolerance. In response, rice planthoppers have developed various adaptive strategies, such as possessing a complex chemosensory system to detect different chemicals, secreting salivary proteins that finely regulate plant defense responses, detoxifying harmful substances through gut-specific enzymes, enhancing adaptability to ecosystems via symbiotic bacteria, and exhibiting wing polyphenism to modulate growth and development according to the nutritional status of the host plant. Based on the latest scientific discoveries on the adaptation mechanisms of rice planthoppers to rice resistance, this paper summarized the genes for rice resistance to planthoppers and the virulence mechanisms of planthoppers. The review focuses on the areas of chemical communication between rice and planthoppers, salivary components, detoxification enzymes, symbiotic bacteria, and wing dimorphism regulation. Studies have shown that biotype evolution of brown planthopper and the loss of resistance in pest-resistant varieties after their promotion remain major challenges in pest management. Future research should prioritize elucidating the molecular mechanisms of adaptability of brown planthoppers and develop new, more effective management strategies to achieve long-term goals and sustainable control of brown planthopper. Additionally, this paper discusses how modern biotechnologies such as genomics, transcriptomics, metabolomics, and epigenetics can be used to deepen the understanding of the interaction network between brown planthoppers and rice, and how this knowledge can be used to formulate more effective pest management strategies.

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    Research Progress in Effectors of Magnaporthe oryzae
    LU Yezi, QIU Jiehua, JIANG Nan, KOU Yanjun, SHI Huanbin
    Chinese Journal OF Rice Science    2025, 39 (3): 287-294.   DOI: 10.16819/j.1001-7216.2025.240611
    Abstract567)   HTML31)    PDF (911KB)(878)       Save

    Rice blast, caused by the fungal pathogen Magnaporthe oryzae, is among the most devastating rice diseases worldwide. During infection, M. oryzae deploys an arsenal of effectors into rice cells, altering their physiology and metabolism, and suppressing immune responses to facilitate colonization. A thorough understanding of M. oryzae effectors and their interaction mechanisms with rice holds significant potential to inform strategies for rice blast control. This review summarizes recent advances in elucidating the mechanisms of effector secretion into rice cells, the intricate regulatory mechanisms of effector-mediated rice blast resistance, and the classification of M. oryzae effectors. Additionally, it discusses the current challenges in effector research and outlines future research directions, aiming to provide a reference for the prevention and management of rice blast caused by M. oryzae.

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    Evaluation of Blast Resistance and Identification of Superior Haplotype of OsDR8 in Rice
    ZHANG Bintao, LIU Congcong, GUO Mingliang, YANG Shaohua, WU Shiqiang, GUO Longbiao, ZHU Yiwang
    Chinese Journal OF Rice Science    2025, 39 (3): 343-351.   DOI: 10.16819/j.1001-7216.2025.240509
    Abstract450)   HTML16)    PDF (1155KB)(640)       Save

    【Objective】Rice (Oryza sativa L.) is a crucial staple crop globally. Its production is severely threatened by rice blast disease. Identifying resistance materials and analyzing resistance genes are essential for understanding the mechanisms behind rice blast resistance. This study aims to systematically generate and identify genetic materials of the rice blast resistance gene OsDR8, conduct haplotype and evolutionary analyses to select superior haplotypes, and investigate their subpopulation differentiation. These efforts will lay a theoretical basis for the application of OsDR8 in rice blast resistance breeding programs.【Method】We developed transgenic lines overexpressing the OsDR8 gene and its knockout mutants. We conducted rice blast resistance assays on the overexpression lines, homozygous knockout lines, and corresponding wild-type materials using Magnaporthe oryzae isolates RO1-1 and RB22. Meanwhile, haplotype and evolutionary analyses of the OsDR8 gene were conducted based on the rice super pan-genome variation map.【Result】Disease symptom observation and disease resistance evaluation indicated that knockout mutants had significantly larger lesion sizes and a higher proportion of diseased leaf area compared to the wild-type. In contrast, the overexpression lines showed reduced lesion sizes and a lower proportion of diseased leaf area. Combined with haplotype and evolutionary analyses, the results indicated that materials of the superior haplotype Hap2 show stronger disease resistance, with 95.52% of the indica rice subpopulation being the Hap2 haplotype.【Conclusion】The OsDR8 gene positively regulates resistance to rice blast, and that the superior haplotype Hap2 may be associated with stronger resistance in indica rice.

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    Effects of Straw Returning Methods on Soil Characteristics and Greenhouse Gas Emissions in Paddy Fields and Their Regulation Through Water-fertilizer Interactions
    WANG Chaorui, ZHOU Yukun, WEN Ya, ZHANG Ying, FA Xiaotong, XIAO Zhilin, ZHANG Hao
    Chinese Journal OF Rice Science    2025, 39 (3): 295-305.   DOI: 10.16819/j.1001-7216.2025.240703
    Abstract436)   HTML27)    PDF (1186KB)(789)       Save

    The rational utilization of crop straw is of great significance to sustain agricultural development, and remarkable progress has been made in the comprehensive utilization of crop straw in China. Returning straw to fields is an effective method for utilizing straw resources, positively impacting grain productivity and promoting a virtuous cycle in soil health. At the same time, returning straw to fields alters the soil characteristics of paddy fields, which in turn changes the composition and activity of soil microorganisms, ultimately affecting greenhouse gas emissions. This work reviewed the effects and mechanisms of straw tillage, no-tillage mulch, and carbonization on soil characteristics and greenhouse gas emissions in paddy fields. It summarized measures for managing water and fertilizer under straw tillage conditions and discussed existing problems and future research priorities.

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    Cloning and Functional Verification of Rice-Blast Resistance Gene Pi-kf2(t) in Kangfeng B
    WEI Xinyu, ZENG Yuehui, XIAO Changchun, HUANG Jianhong, RUAN Hongchun, YANG Wangxing, ZOU Wenguang, XU Xuming
    Chinese Journal OF Rice Science    2025, 39 (3): 352-364.   DOI: 10.16819/j.1001-7216.2025.240502
    Abstract413)   HTML7)    PDF (3338KB)(448)       Save

    【Objective】Rice blast is a worldwide disease caused by filamentous ascomycete fungus Magnaporthe oryzae, which seriously affects the quality and yield of rice. At present, cultivating and planting disease-resistant varieties is the most economical and effective strategy to control rice blast, and the mining and utilization of blast-resistance genes is the key to disease-resistant rice breeding. In this study, Kangfeng B (KFB), an elite maintainer line of indica hybrid rice with broad-spectrum resistance derived from Sanming Academy of Agricultural Sciences was used as the test material, and the Pi-kf2(t) gene in which was used to conduct resistance spectrum analysis, cloning and functional verification.【Method】For resistance spectrum analysis, KFB (Pi-kf2(t)), 75-1-127 (Pi9), C101A51 (Pi2), IRBLzt-T (Piz-t), EBZ (Pi50) and GM-4 (Pigm) seedlings were inoculated with 100 rice blast isolates from different regions of China during 3-4 leaf stages; The expression analysis of two Pi-kf2(t) candidate genes, Pi-kf2(t)-NBS2 and Pi-kf2(t)-NBS4, were determined by semi-quantitative reverse-transcription PCR (RT-PCR) and quantitative real-time PCR (qRT-PCR); Pi-kf2(t) gene was cloned by homologous cloning strategy and a phylogenetic tree was constructed to investigate the possible relationships between Pi-kf2(t) and Pi9, Pi2, Piz-t, Pi50, and Pigm proteins in evolutionary history. Moreover, sequence and expression analyses revealed Pi-kf2(t)-NBS2 as the candidate Pi-kf2(t) gene, which was verified by transgenic complementation test.【Result】The resistance spectrum of Pi-kf2(t), Pi9, Pi2, Piz-t, Pi50 and Pigm were 93%, 90%, 91%, 78%, 95% and 96%, respectively. The candidate gene Pi-kf2(t)-NBS2 exhibited a high expression level in the leaves of KFB seedlings and was not induced by isolates of rice blast, which showed a constitutive expression, while Pi-kf2(t)-NBS4 exhibited an extremely poor expression. Multiple amino acid sequence alignment analysis revealed that there were 45, 31, 34, 2, and 2 amino acids differences between Pi-kf2(t)-NBS2 and Pi9, Pi2, Piz-t, Pi50, and Pigm proteins, respectively. Phylogenetic tree analysis showed that Pi-kf2(t)-NBS2 exhibited a closer genetic relationship and a higher homology with Pi50 and Pigm. Furthermore, genetic complementation test verified that the candidate gene Pi-kf2(t)-NBS2 was the Pi-kf2(t).【Conclusion】The rice blast-resistance gene Pi-kf2(t) is a novel member of the Pi2/Pi9 multigene family. This work provided a theoretical basis for further disease resistance mechanism study of Pi-kf2(t), and also provided an important germplasm resource for rice blast-resistance breeding.

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    Development and Application of KASP Marker for Broad-Spectrum Resistance Gene PigmR to Rice Blast
    LI Wenqi, XU Yang, WANG Fangquan, ZHU Jianping, TAO Yajun, LI Xia, FAN Fangjun, JIANG Yanjie, CHEN Zhihui, YANG Jie
    Chinese Journal OF Rice Science    2025, 39 (3): 365-372.   DOI: 10.16819/j.1001-7216.2025.240104
    Abstract393)   HTML9)    PDF (1529KB)(589)       Save

    【Objective】The broad-spectrum resistance gene PigmR has important breeding value for the genetic improvement of rice blast resistance. Developing kompetitive allele specific PCR (KASP) markers based on its coding region variation sites is beneficial for improving the accuracy and efficiency of PigmR utilization in breeding.【Method】This study identified single nucleotide variations in the coding region of PigmR through sequence alignment, developed the KASP molecular marker PigmR-K, conducted genotype analysis using rice varieties and F2 segregating populations, combined these results with rice blast symptom identification, and carried out molecular marker assisted selection breeding to verify the precision and efficiency of this marker.【Result】Based on an A-to-C mutation at position 2503 in the PigmR gene coding region sequence, a precise and efficient PigmR genotyping technology system based on KASP markers was established. The genotyping of 159 rice varieties, 270 F2 individuals, and 247 BC1F3 population lines demonstrated that PigmR-K reliably distinguished the homozygous resistant, homozygous susceptible and heterozygous genotypes of PigmR. These results were completely consistent with disease symptoms from artificial panicle blast inoculation.【Conclusion】The KASP marker PigmR-K developed based on the variation site of on the coding region has advantages such as precision and efficiency, which is beneficial for genetic improvement of rice blast resistance.

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    Functional Study on Aluminum Tolerance of OsAlR1 Gene in Rice
    WANG Jingbo, SU Chang, FENG Jing, JIANG Sixu, XU Hai, CUI Zhibo, ZHAO Minghui
    Chinese Journal OF Rice Science    2025, 39 (5): 615-623.   DOI: 10.16819/j.1001-7216.2025.240510
    Abstract388)   HTML11)    PDF (1429KB)(346)       Save

    【Objective】 With the aggravation of soil acidification, aluminum (Al) toxicity in acidic soil seriously limits the growth and development of rice. Mining Al-tolerant gene resources, analyzing their molecular functions, and breeding Al-tolerant varieties can ensure rice yield in acidic soil. 【Method】 CRISPR/Cas9 technology was used to construct knockout vectors. The overexpression vector was constructed by homologous recombination and Golden Gate seamless cloning. Transgenic materials were obtained by genetic transformation. Homozygous overexpression lines and knockout lines were screened for Al tolerance phenotype analysis and determination of reactive oxygen species (ROS) content and antioxidant enzyme activities.【Result】 qRT-PCR results showed that OsAlR1 expression was induced by Al stress. Knockout of OsAlR1 affected root development in rice. After Al treatment, compared with the wild type, the osalr1-3 and osalr1-6 lines accumulated more Al³⁺ and ROS, while the OE-OsAlR1-5 and OE-OsAlR1-8 lines accumulated less Al³⁺ and ROS. Knockout of OsAlR1 led to decreased glutathione (GSH) content and superoxide dismutase (SOD) activity in roots. qRT-PCR results revealed that genes regulating the phenylpropanoid biosynthesis pathway (OsPAL7, OsHCT4, OsCCR18, Os02g0467600, PRX82, and Os06g0522300) showed significant expression changes in the knockout lines after Al treatment.【Conclusion】 OsAlR1 expression is induced by Al stress, and OsAlR1 alleviates ROS-mediated oxidative stress by increasing GSH content and SOD activity. OsAlR1 may affect Al tolerance in rice by regulating the phenylpropanoid biosynthesis pathway.

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    Advances in Roles of Different Types of Histone Modifications in Responses of Rice to Abiotic Stresses
    HAO Wenqian, CAI Xingjing, YANG Haidong, WU Yuyang, TENG Xuan, XUE Chao, GONG Zhiyun
    Chinese Journal OF Rice Science    2025, 39 (5): 575-585.   DOI: 10.16819/j.1001-7216.2025.250203
    Abstract383)   HTML28)    PDF (1171KB)(2030)       Save

    As one of the most important food crops in the world, the yield and quality of rice are directly related to global food security. However, rice often faces various abiotic stresses during growth, such as salt stress, drought, low and high temperatures, which seriously compromise the growth, development and yield of rice. In recent years, epigenetics research, especially the role of histone modifications in regulating stress tolerance responses of rice, has attracted increasing attention. This review summarizes the latest research progress in histone modifications in abiotic stress responses in rice, to provide a theoretical basis for genetic improvement and stress resistance breeding of rice.

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    Research Progress on Physiological and Ecological Mechanisms and Regulation Pathways of Yield, Quality and Stress Resistance Response in Perennial Rice
    LU Tingting, YAN Wenhui, SU Xinquan, ZENG Luohua, HUA Liqin, CHEN Jianghua, FENG Baohua, WANG Yuexing, HU Jiang, FU Guanfu
    Chinese Journal OF Rice Science    2025, 39 (5): 586-600.   DOI: 10.16819/j.1001-7216.2025.241012
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    Perennial rice, characterized by its "one planting, two or more harvests", has become a crucial component of sustainable agriculture. Significant progress has been made in understanding the physiological and ecological mechanisms and regulation pathways of perennial rice. This study aims to systematically review the current status, yield and quality formation characteristics, physiological and ecological characteristics, and molecular mechanisms of perennial rice, with an in-depth discussion on its response mechanisms to stress conditions. Finally, we summarize the cultivation patterns and regulation strategies of perennial rice, providing theoretical foundations and practical guidance for expanding its planting regions and enhancing the diversity of rice germplasm resources.

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    Functional Study of Rice Eukaryotic Translation Initiation Factor OseIF6.2 in Grain Size Regulation
    MA Shunting, HU Yungao, GAO Fangyuan, LIU Liping, MOU Changling, LÜ Jianqun, SU Xiangwen, LIU Song, LIANG Yuyu, REN Guangjun, GUO Hongming
    Chinese Journal OF Rice Science    2025, 39 (3): 331-342.   DOI: 10.16819/j.1001-7216.2025.2401010
    Abstract372)   HTML12)    PDF (1741KB)(1469)       Save

    【Objective】This study aims to investigate the functional role of the rice eukaryotic translation initiation factor OseIF6.2 in rice growth and development using a reverse genetics approach, thereby laying a foundation for further exploration of the functions of OseIF6.2.【Method】First, bioinformatics methods were employed to perform multiple sequence alignment and a phylogenetic tree was constructed to analyze the evolutionary relationship between OseIF6.2 and eIF6 proteins in other eukaryotes. CRISPR/Cas9 gene editing technology was used to precisely edit the OseIF6.2 gene in rice (Nipponbare).Next, the expression levels of OseIF6.2 in different tissues of rice were detected using quantitative real-time PCR (qRT-PCR) technology. The precise localization of OseIF6.2 within cells was observed using a confocal laser microscope.Finally, the interaction between OseIF6.2 and its potential interacting protein, OseIF6.1, was validated using yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays. 【Result】Phylogenetic analysis revealed that OseIF6.2 is highly conserved among eukaryotic eIF6 proteins, underscoring its universal role in translation regulation.qRT-PCR results indicated that OseIF6.2 expression was relatively high in rice stems. Subcellular localization analysis using GFP-tagged OseIF6.2 in tobacco leaf cells showed fluorescence signals in both the nucleus and cytoplasm, confirming its dual localization.Additionally, compared to the wild type, the oseif6.2 mutant exhibited significant reductions in grain length, grain thickness, and 1000-grain weight. Both Y2H and BiFC experiments confirmed a direct interaction between OseIF6.2 and OseIF6.1【Conclusion】In summary, OseIF6.2, a eukaryotic translation initiation factor in rice, plays a crucial role in regulating grain morphology, further highlighting its importance in rice growth, development, and grain formation.

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    Plant Prime Editing: A New Direction in Crop Breeding
    SONG Anqi, WU Songquan, MA Qiuyue, BAN Wanning, LIU Xiangguo, JIN Yongmei
    Chinese Journal OF Rice Science    2025, 39 (6): 711-730.   DOI: 10.16819/j.1001-7216.2025.241005
    Abstract372)   HTML29)    PDF (2292KB)(368)       Save

    Plant Prime Editing (PPE) provides a novel approach for precise genome modification in plants, overcoming the limitations of conventional gene editing methods that rely on double-strand breaks and exogenous donor DNA. It enables not only arbitrary base substitutions, but also precise insertions and deletions of small to large DNA fragments, with reduced off-target effects. This review systematically summarizes the latest research advances in PPE technology globally, including its development history and working mechanism, applications in crop breeding and trait improvement, and representative cases. It also discusses the expansion of prime editing into large-scale genome editing and multiplex gene editing. To address the bottlenecks faced by PPE systems in plant genetic transformation, several potential solutions are proposed. Finally, the broad application prospects of PPE technology in plant genetic improvement and its potential integration with artificial intelligence (AI) in future research are outlined.

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    Grain-filling Characteristics and Its Relationship with Grain Yield Formation of japonica Rice Nanjing 9108 Under Combined Salinity-drought Stress
    WEI Huanhe, WANG Lulu, MA Weiyi, ZHANG Xiang, ZUO Boyuan, GENG Xiaoyu, ZHU Wang, ZHU Jizou, MENG Tianyao, CHEN Yinglong, GAO Pinglei, XU Ke, DAI Qigen
    Chinese Journal OF Rice Science    2025, 39 (3): 373-386.   DOI: 10.16819/j.1001-7216.2025.240515
    Abstract368)   HTML14)    PDF (2326KB)(1165)       Save

    【Objective】This study aims to investigate the effects of combined salinity-drought stress on rice grain-filling characteristics and its relationship with yield formation.【Method】The study was conducted with conventional japonica rice cultivar Nanjing 9108, which is widely cultivated in the coastal saline soil of Jiangsu Province, China. The experimental design consisted of factorial combinations of salinity and drought stresses: salinity stress induded non-salinity (NS, salt concentration 0%) and high-salinity stress (HS, salt concentration 0.3%); while drought regimes comprised well-watered (WW), moderate drought (MD), and severe drought (SD) treatments from jointing stage to heading stage.【Result】Compared with non-stress, 1) the yield reduction under salinity stress, drought stress, and combined salinity-drought stress was 36.8%, 13.7%−30.0%, and 60.3%−107.1%, respectively (two-year average). 2) the number of panicles per unit area, grains per panicle, seed setting rate, and the thousand-grain weight were all lower under single salinity stress, single drought stress, and combined salinity-drought stress, and the reduction was greater under combined salinity-drought stress; the panicle length, and grain weight and seed setting rate of superior and inferior grains showed a similar trend. 3) single salinity stress, single drought stress, and combined salinity-drought stress significantly reduced dry matter weight at jointing and heading and the accumulation of dry matter weight from heading to maturity, as well as leaf photosynthetic rate and SPAD value at 20 and 40 days after heading; the reductions under combined salinity-drought stress were greater than those under single salinity and single drought stress. 4) the maximum grain-filling rate and the mean grain-filling rate of superior and inferior grains were lower under single salinity stress, single drought stress, and combined salinity-drought stress, while the time to reach the maximum grain-filling rate and the effective grain-filling period of superior grains showed an increasing trend, and the differences in the time to reach the maximum grain-filling rate and the effective grain-filling period of inferior grains were smaller. The number of days in the early, middle, and late periods were greater under single salinity stress, single drought stress, and combined salinity-drought stress, while lower for the mean grain-filling rate and grain-filling amount. 5) single salinity stress, single drought stress, and combined salinity-drought stress treatments reduced the activities of key starch synthesis enzymes in grains, especially for AGPase and GBSS enzymes.【Conclusion】This study demonstrats that the grain-filling rate and key starch synthesis enzyme activities of superior and inferior grains decreased significantly and the grain-filling characteristics of superior and inferior grains were deteriorated under single salinity stress, single drought stress, and especially combined salinity-drought stress. The inhibitory effects of combined salinity-drought stress on the grain-filling rate, grain-filling amount of superior and inferior grains, and grain yield were greater than single salinity and drought stress.

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    Research Progress in Regulation of Anthocyanin Accumulation in Colored Rice
    WU Wanting, XU Qian, LIU Dantong, ZHU Changjin, DU Haotian, JU Haoran, HUO Zhongyang, DAI Qigen, LI Guohui, XU Ke
    Chinese Journal OF Rice Science    2025, 39 (5): 601-614.   DOI: 10.16819/j.1001-7216.2025.241206
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    Colored rice is rich in protein, amino acids, cellulose, and essential minerals for the human body. It also contains active substances such as anthocyanins, which have anti-aging, cancer prevention, and antiviral effects, making it widely valued by consumers. This review focuses on summarizing the nutritional and health value of colored rice, and summarizes the accumulation, biosynthesis, and regulation processes of anthocyanins in colored rice from the perspectives of physiological and molecular regulation, as well as the effects of cultivation conditions such as temperature, light, water, and fertilizer level on the accumulation of anthocyanins. Finally, future research directions for colored rice are discussed.

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    Effects of Phosphorus Fertilizer Application Rate on Grain Yield, Phosphorus Accumulation and Utilization of Direct-seeded Rice in Hubei Province
    SHEN Zhida, YU Qiuhua, ZHANG Bin, CAO Yudong, WANG Shaohua, WANG Hongfei, WU Yongqing, DAI Zhigang, LI Xiaokun
    Chinese Journal OF Rice Science    2025, 39 (3): 399-411.   DOI: 10.16819/j.1001-7216.2025.240512
    Abstract367)   HTML9)    PDF (1709KB)(991)       Save

    【Objective】This study aims to clarify the effect of phosphorus fertilizer dosage on grain yield, phosphorus accumulation, phosphorus fertilizer utilization, and economic benefits of direct-seeded rice in Hubei Province. It provides a theoretical basis for the scientific fertilization of direct-seeded rice in the region.【Methods】Four phosphorus fertilizer application rates (0, 45, 90, and 135 kg/hm²)were established in a multi-site experiment across counties (cities and districts) in the major rice-producing areas of Hubei Province. The grain yield, phosphorus content, phosphorus accumulation, phosphorus fertilizer utilization, and economic benefits of rice under different phosphorus fertilizer dosages were comparatively analyzed.【Results】Compared to the zero phosphorus treatment (0 kg/hm²), the phosphorus fertilizer dosages of 45, 90, and 135 kg/hm² at each experimental site increased the grain yield by 0.18−1.80 t/hm², 0.30−3.24 t/hm², and 0.60−2.61 t/hm², respectively, with average increases of 9.15%, 16.08%, and 18.24%.Additionally, the aboveground phosphorus accumulation of rice increased by 1.57−9.18 kg/hm², 2.67−12.77 kg/hm², and 4.16−15.06 kg/hm², with average increases of 29.14%, 44.92%, and 53.60%, respectively. Overall, rice yield increased with the phosphorus fertilizer dosage in the range of 0−90 kg/hm², and there was no significant difference in rice yield when the phosphorus fertilizer dosage exceeded 90 kg/hm².With the increase in phosphorus fertilizer dosage, rice phosphorus accumulation, production value, and net income followed a trend of increasing and then stabilizing, while partial productivity of phosphate fertilizer, agronomic utilization rate, recycling rate, and production-to-input ratio decreased with higher phosphorus fertilizer dosages. An interaction was observed between phosphorus fertilizer dosage and experimental location regarding yield, phosphorus accumulation, phosphorus fertilizer utilization, production value, and net income of direct-seeded rice.【Conclusion】Appropriate phosphorus fertilizer dosage can significantly improve the grain yield, aboveground phosphorus accumulation, and phosphorus fertilizer utilization of direct-seeded rice. Considering yield, phosphorus accumulation, fertilizer utilization, and economic benefits, the recommended dosage of phosphorus fertilizer for direct-seeded rice in Hubei Province is 58−104 kg/hm², with an average of 82 kg/hm². The theoretical maximum yield can reach 6.97−12.18 t/hm², with an average of 9.14t/hm², which can ensure rice yield while minimizing nutrient loss and reducing environmental pollution.

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    Research Progress of Typical Plant Growth-promoting Microorganism Enhancing Salt Stress Resistance in Rice
    ZHOU Yang, YE Fan, LIU Lijun
    Chinese Journal OF Rice Science    2025, 39 (4): 529-542.   DOI: 10.16819/j.1001-7216.2025.240814
    Abstract363)   HTML17)    PDF (1685KB)(711)       Save

    Soil salinization is one of the main challenges facing global food security. Rice is the preferred grain crop for the reclamation of coastal tidal flats and saline-alkaline land. Enhancing salt tolerance is essential to ensuring stable saline-alkaline soil rice production. Plant growth-promoting microorganisms (PGPMs) are a crucial part of the biological improvement of saline-alkaline soils. Salt-tolerant PGPMs, such as arbuscular mycorrhizal fungi, Bacillus, and Pseudomonas, can alleviate the adverse effects of salt stress on the growth and development of rice by activating soil enzyme activity, producing extracellular polysaccharides, enhancing antioxidant enzyme activity, regulating osmotic metabolism osmoprotectants, and regulating plant hormones. This work summarizes different pathways and related mechanisms by which the three aforementioned PGPMs enhance rice resistance under salt stress, highlights the current research gaps in this field, and provides an outlook for future research, thereby offering theoretical and practical bases for the improvement of saline-alkaline soils and high-yield rice production.

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    Research on Path Tracking Control Methods for Tractor Operating Units: A Review
    XIAO Maohua, TIAN Fengyu, WEI Wenbo, ZHU Yejun, LI Dongfang, ZHANG Pengcheng, GENG Guosheng
    Chinese Journal OF Rice Science    2025, 39 (4): 423-439.   DOI: 10.16819/j.1001-7216.2025.250104
    Abstract358)   HTML14)    PDF (1823KB)(1378)       Save

    The tractor operating unit refers to an agricultural machinery system with a unique articulated tractor structure composed of a tractor and supporting implements, and the path tracking control method is the key technology to realize the unmanned operation of this unit. However, limited by the variability of the agricultural environment and the complex steering structure of the articulated frame, the existing methods still face challenges in terms of adaptability, robustness, and control efficiency. The aim of this work is to provide a systematic overview of current research status on path tracking control methods for tractor operating units by analyzing their models and path tracking control algorithms. In addition, this review discusses the main problems in research on path tracking control methods for tractor operating units and possible solutions, with a view to providing useful references for related research in this field.

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    Directed Improvement of Hybrid Rice Zhuoliangyou 1126 by Heavy Ion Beam Mutagenesis Based on M1TDS Targeted Screening Technology
    SHAO Ye, HU Yuanyi, PENG Yan, MAO Bigang, LIU Huimin, TANG Chanjuan, LEI Bin, TANG Li, YU Lixia, LI Wenjian, LUO Wuzhong, LUO Zhibin, YUAN Yuantao, LI Yaokui, ZHANG Dan, ZHOU Libin, BAI Lianyang, TANG Wenbang, ZHAO Bingran
    Chinese Journal OF Rice Science    2025, 39 (5): 624-634.   DOI: 10.16819/j.1001-7216.2025.240106
    Abstract354)   HTML11)    PDF (2114KB)(1115)       Save

    Objective】Using the two-line hybrid rice Zhuoliangyou 1126 as the base variety, we aimed to rapidly develop upgraded varieties with traits such as low cadmium accumulation, fragrance, storage tolerance, submergence tolerance, and a low glycemic index.【Method】Zhuo 234S and Xiangnonghui 1126, the parental lines of Zhuoliangyou 1126, were mutagenized using heavy-ion beams. Chimeric mutants of the OsNRAMP5, OsBADH2, OsLOX3, OsPAO5, OsSSIIIa, and OsBEIIb genes were identified in the M1 generation via M1TDS technology. Mutations in the target genes were further detected and isolated in the M2 generation using Kompetitive Allele-Specific PCR (KASP) genotyping. Hybrid rice with low cadmium accumulation was developed by crossing parents carrying OsNRAMP5 mutations.【Results】A total of 13 chimeric mutants involving six target genes were identified in the M1 generation. Among these, seven were confirmed to carry homozygous or heterozygous mutations in the M2 generation. The cadmium content in OsNRAMP5 homozygous mutants was significantly lower than in the wild type, while the content of the aromatic compound 2-acetylpyrroline (2-AP) was significantly higher in OsBADH2 homozygous mutants. When the low-cadmium hybrid rice derived from OsNRAMP5-mutated parents was pot-grown in cadmium-contaminated soil (available Cd: 0.677 mg/kg, pH 5.6), the cadmium content in grains remained consistently below 0.05 mg/kg, compared to 0.91 mg/kg in the wild-type control.【Conclusion】The combination of heavy-ion beam mutagenesis and M1TDS technology enabled the rapid improvement of traits such as low cadmium accumulation in Zhuoliangyou 1126. This study provides a successful example and a general technical reference for transitioning from traditional mutagenesis breeding to directed mutagenesis breeding.

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    Evaluation of SNP Loci for Rice Variety Authenticity Verification
    XU Qun, WANG Shan, YUAN Xiaoping, JIN Shiqiao, JIN Fang, HAO Wanjun, WU Xiaobi, FENG Yue, YU Hanyong, SUN Yanfei, YANG Yaolong, WEI Xinghua
    Chinese Journal OF Rice Science    2025, 39 (5): 635-642.   DOI: 10.16819/j.1001-7216.2025.241114
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    【Objective】 A highly discriminative combination of SNP loci is crucial for developing efficient, accurate, and cost-effective rice variety verification technologies, which can be widely applied in seed regulation, variety breeding and other fields.【Method】 This study established fingerprints for 1,383 inbred and 2,702 hybrid rice varieties using the 96 SNP panel from the agricultural industry standard, designated RGIsnp96.【Result】 The results demonstrated that RGIsnp96 exhibited high polymorphism and strong discriminative power for variety identification. For 1,387 inbred varieties, the heterozygous rate, average polymorphic information content (PIC), and average minor allele frequency (MAF) of SNPs in RGIsnp96 were 0.03, 0.30, and 0.27, respectively. For 2702 hybrid varieties, the corresponding values were 0.39, 0.30, and 0.29, respectively. The identification rates, represented by the proportion of distinguishable variety pairs, for inbred and hybrid rice varieties were 99.94% and 99.99%, respectively. A good equivalence between RGIsnp96 and 48 SSRs in agricultural industry standards (designated RGIssr48 in the following) was evaluated by comparing similarities of variety pairs measured by the two marker sets for both inbred and hybrid varieties. Furthermore, combining RGIsnp96 and RGIssr48, representing different types of markers, could generate better results than using a single type of marker for differentiating certain inbred rice varieties, and corresponding strategies were recommended.【Conclusion】 RGIsnp96 demonstrates exceptional capability in rice variety identification, providing robust technical support for improving rice seed quality, regulating the seed market order, and safeguarding farmers' rights and interests.

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    Effects of Nitrogen Type of Basal Fertilizer on Growth, Grain Yield and Nitrogen Use Efficiency of Ratooning Rice
    ZHANG Haiwei, GU Xinyi, CHEN Mingshuai, LI Fukang, SHI Yuecheng, YANG Ting, JIANG Shuochen
    Chinese Journal OF Rice Science    2025, 39 (3): 387-398.   DOI: 10.16819/j.1001-7216.2025.240507
    Abstract292)   HTML7)    PDF (1384KB)(441)       Save

    【Objective】This study investigated the effects of different nitrogen types of basal fertilizer on root function, photosynthetic capacity, nitrogen metabolism enzyme activity, grain yield, and nitrogen use efficiency of ratooning rice, aiming to provide a theoretical basis for high-yield and efficient production of ratooning rice in the Jianghan Plain.【Method】A two-year field experiment (2020-2021) was conducted at the Experimental Station of Yangtze University in Jingzhou City, Hubei Province. Four basal fertilizer treatments were applied: no nitrogen application (N0), urea (CK), 50% controlled release urea + 50% urea (T1), and 50% livestock manure organic fertilizer + 50% urea (T2). The N0 treatment received no nitrogen throughout the rice growth season, while other fertilization periods maintained consistent nitrogen types and amounts across CK, T1, and T2. Root dry weight, root activity, leaf area index (LAI), and net photosynthetic rate (Pn) were measured at critical growth stages. Activities of nitrogen metabolism enzymes were determined at heading stages of both main and ratooning seasons. Yield components and nitrogen use efficiency parameters were analyzed.【Result】The T2 treatment exhibited the highest root dry weight, root activity, LAI, and Pn. Activities of nitrogen metabolism enzymes and yields for both seasons followed the order: T2 > T1 > CK > N0. Compared with N0, total rice yield increased by 75.13%, 97.37%, and 137.86% under CK, T1, and T2 treatments, respectively. Relative to CK, T1 and T2 significantly improved grains per panicle, seed-setting rate, and 1000-grain weight. Nitrogen recovery efficiency, agronomic nitrogen use efficiency, and partial factor productivity of nitrogen ranked as T2 > T1 > CK.【Conclusion】Replacing 50% conventional urea with livestock manure organic fertilizer as base fertilizer enhanced root function, photosynthetic capacity, and nitrogen metabolism enzyme activities, resulting in the highest yield and nitrogen use efficiency. This strategy represents an optimal nitrogen management approach for ratooning rice production in the Jianghan Plain.

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    Transcriptome Analysis of Top Second Leaf Sheath of Rice Under Different Nitrogen Fertilizer Levels
    HUANG Fudeng, WU Chunyan, HAO Yuanyuan, HAN Yifei, ZHANG Xiaobin, SUN Huifeng, PAN Gang
    Chinese Journal OF Rice Science    2025, 39 (4): 563-574.   DOI: 10.16819/j.1001-7216.2025.250306
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    【Objective】 The leaf sheath is an important organ that simultaneously serves the functions of “source, sink, and transport”, playing a crucial role in the growth and development of rice plants. Identifying important genes in leaf sheaths in response to nitrogen will provide scientific evidence for the genetic improvement of nitrogen use efficiency in rice. 【Method】 The japonica rice cultivar Xiushui 134 was planted in percolation ponds at low nitrogen (LN), medium nitrogen (MN), and high nitrogen (HN) levels. The top second leaf sheaths were collected on the 10th day after heading for transcriptome sequencing analysis to identify important genes involved in the nitrogen response of rice leaf sheaths. 【Result】 Comparative analysis of the transcriptome data revealed 1,791 differentially expressed genes at the three nitrogen levels. The number of upregulated and downregulated genes between HN and MN, MN and LN, and HN and LN were 312 and 155, 263 and 160, and 1,059 and 542, respectively. The common upregulated and downregulated genes at the three nitrogen levels were 15 and 53, respectively, involved in physiological and biochemical processes such as nutrient element absorption and transport, responses to biotic and abiotic stresses, plant hormone responses, and light regulation. Based on GO enrichment analysis, KEGG metabolic pathway analysis, a total of 6 known functional genes related to nitrogen utilization and 4 genes related to photosynthesis, as well as 50 genes of unknown function, were identified. It is speculated that these genes play important roles in the nitrogen response of the second leaf sheath in rice. 【Conclusion】 The research results preliminarily clarify the important genes involved in the leaf sheath's response to different nitrogen levels, which may play significant roles in signal transduction pathways of nitrogen utilization.

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