Chinese Journal OF Rice Science

Chinese Journal of Rice Science

• 实验技术 • Previous Articles     Next Articles

A Rapid DNA Miniprep Extraction Method for Large-Scale Rice Mutant Screening

QIU Fu-lin , WANG He-he , CHEN Jie , ZHUANG Jie-yun , Hei LEUNG , CHENG Shi-hua , WU Jian-li
  

  • Received:1900-01-01 Revised:1900-01-01 Online:2006-05-10 Published:2006-05-10

用于水稻突变体大量筛选的DNA微量快速提取法

邱福林1,2,3;王和和4;陈洁2;庄杰云2;Hei LEUNG4;程式华2,* ;吴建利2,*   

  1. 1沈阳农业大学， 辽宁 沈阳 110161; 2中国水稻研究所 国家水稻改良中心/水稻生物学国家重点实验室， 浙江 杭州 310006； 3辽宁省农业科学院 水稻研究所， 辽宁 沈阳 110101； 4国际水稻研究所 昆虫和植物病理系， 菲律宾 马尼拉； *通讯联系人， E-mail: shcheng@mail.hz.zj.cn; jianliwu@gmail.com

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Abstract: A high throughput rice DNA mini-preparation method was developed. The method is suitable for large-scale mutant bank screening as well as large mapping populations with characteristics of maintaining relatively high level of DNA purity and concentration. The extracted DNA was tested and suitable for regular PCR amplification (SSR) and TILLING(targeting induced local lesion in genome) analysis.

Key words: rice, targeting induced local lesion in genome, DNA extraction, polymerase chain reaction

摘要： 介绍了一种高通量水稻DNA微量快速抽提法。该方法特别适用于精细作图群体和大型突变体库的筛选鉴定，不仅通量大、速度快，而且可以确保足够的DNA浓度和纯度。DNA质量可以确保一般的PCR扩增，包括SSR检测以及应用于TILLING（targeting induced local lesion in genome）分析。

关键词: 水稻, 点突变检测（TILLING）