Breeding Restore Lines of Hybrid Rice by Pyramiding Genes for Resistance to White Backed Planthoppers and Brown Planthoppers

doi:10.16819/j.1001-7216.2019.9007

Chinese Journal OF Rice Science ›› 2019, Vol. 33 ›› Issue (5): 421-428.DOI: 10.16819/j.1001-7216.2019.9007

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Breeding Restore Lines of Hybrid Rice by Pyramiding Genes for Resistance to White Backed Planthoppers and Brown Planthoppers

Yongsheng ZHU^1,², Jianlin BAI², Hongguang XIE¹, Fangxi WU¹, Xi LUO¹, Shenfei JIANG¹, Wei HE¹, Liping CHEN¹, Qiuhua CAI¹, Huaan XIE^1,², Jianfu ZHANG^1,^2,^*()

¹Rice Research Institute, Fujian Academy of Agricultural Sciences/Research Bases of South-China, National Key Laboratory of Hybrid Rice of China/Key Laboratory of Hybrid Rice Germplasm Enhancement and Molecular Breeding in South China, Ministry of Agriculture and Rural Affairs, P. R. China/National Engineering Laboratory of Rice for China/ Fujian Engineering Laboratory of Crop Molecular Breeding/ Fujian Key Laboratory of Rice Molecular Breeding, Fuzhou 350003, China
²College of Plant Protection, Fujian Agriculture and Forestry University/State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fuzhou 350002, China

Received:2019-01-10 Revised:2019-03-18 Online:2019-09-10 Published:2019-09-10
Contact: Jianfu ZHANG

聚合白背飞虱和褐飞虱抗性基因创制杂交水稻恢复系

朱永生^1,², 白建林², 谢鸿光¹, 吴方喜¹, 罗曦¹, 姜身飞¹, 何炜¹, 陈丽萍¹, 蔡秋华¹, 谢华安^1,², 张建福^1,^2,^*()

¹福建省农业科学院水稻研究所/杂交水稻国家重点实验室华南研究基地/农业农村部华南杂交水稻种质创新与分子育种重点实验室/水稻国家工程实验室/福建省作物分子育种工程实验室/福建省水稻分子育种重点实验室, 福州 350003
²福建农林大学植物保护学院/闽台作物有害生物生态防控国家重点实验室, 福州 350002

通讯作者: 张建福
基金资助:
国家重点研发计划资助项目(2017YFD0100100);福建省公益类科研院所科研专项(2015R1021-5);闽台作物有害生物生态防控国家重点实验室开放课题基金资助项目(SKB2017001);福建省农业科学院创新团队项目(STIT2017-1-1);福建省农业科学院科技服务团队资助项目(kjfw02)

Abstract

Abstract:

【Objective】To breed restore lines of hybrid rice resistant to white backed planthoppers and brown planthoppers,【Method】we used B5 with brown planthopper resistance genes Bph14 and Bph15 and Fuhui 7011 with white backed planthoppers resistance gene qsI-4 as donor parents, and Fuhui 676, a main restorer line, as the recurrent parent through the molecular marker-assisted selection combined with field and indoor identification. The insect-resistant lines and their hybrid progenies were tested and their agronomic traits were analyzed. 【Result】Three restorer lines with three resistance genes to rice brown planthoppers and white backed planthoppers (Bph14, Bph15 and qsI-4) and three lines with two resistance genes were bred. Resistance identification indicated that the resistance of the restorer lines was moderately resistant or resistant. Two restorer lines with potential for production and application were screened out by examining the resistant strains and their crossing progenies and analyzing their agronomic traits.【Conclusion】This study provides new resistance germplasms against brown planthoppers and white backed planthoppers for hybrid rice breeding.

Key words: rice, white backed planthopper(WBPH), brown planthopper(BPH), resistance gene, pyramiding breeding

摘要：

【目的】 为了创制兼抗白背飞虱和褐飞虱的水稻恢复系,【方法】 分别以抗褐飞虱材料B5（携带褐飞虱抗性基因Bph14和Bph15）及携带白背飞虱抗性位点qsI-4的籼型恢复系福恢7011为供体亲本,以骨干恢复系福恢676为轮回亲本,应用低世代分离群体田间表型结合单株鉴定与高世代稳定株系室内筛选和分子标记辅助选择相结合的方法,并对抗虫株系及其测交后代进行考查和农艺性状分析。【结果】 选育出聚合Bph14、Bph15 和qsI-4的恢复系材料3份,携带2个抗虫基因的恢复系材料3份。其中6份恢复系的褐飞虱抗性鉴定结果均表现中抗以上。通过抗性鉴定和杂交后代农艺性状分析筛选出具有生产应用潜力的恢复系材料2份。【结论】 为褐飞虱和白背飞虱抗性聚合新种质的创制和应用提供了基础材料。

关键词: 水稻, 白背飞虱, 褐飞虱, 抗性基因, 聚合育种

CLC Number:

Yongsheng ZHU, Jianlin BAI, Hongguang XIE, Fangxi WU, Xi LUO, Shenfei JIANG, Wei HE, Liping CHEN, Qiuhua CAI, Huaan XIE, Jianfu ZHANG. Breeding Restore Lines of Hybrid Rice by Pyramiding Genes for Resistance to White Backed Planthoppers and Brown Planthoppers[J]. Chinese Journal OF Rice Science, 2019, 33(5): 421-428.

朱永生, 白建林, 谢鸿光, 吴方喜, 罗曦, 姜身飞, 何炜, 陈丽萍, 蔡秋华, 谢华安, 张建福. 聚合白背飞虱和褐飞虱抗性基因创制杂交水稻恢复系[J]. 中国水稻科学, 2019, 33(5): 421-428.

Figures/Tables 9

References 25

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基因 Gene	标记性质 Characteristics	染色体 Chromosome	标记 Marker	类型 Type	标记引物序列 Primer sequence（5′-3′）	解链温度 Melting temperature /℃	产物大小 Production size/bp
qSI-4	共显性 Codominant	4	RM8213	SSR	F: AGCCCAGTGATACAAAGATG R: GCGAGGAGATACCAAGAAAG	55	208
Bph14	共显性 Codominant	3	RI35	InDel	F: CAATCATCAAGCACGCGTTA R: ATCGAAGCCACTTGGTGAAC	58	325
Bph15	共显性 Codominant	4	Y15	InDel	F: AGGAACAGTGACACGTAGCA R: GGAGAGTTCAGTTTGCCATCC	55	460

基因 Gene	标记性质 Characteristics	染色体 Chromosome	标记 Marker	类型 Type	标记引物序列 Primer sequence（5′-3′）	解链温度 Melting temperature /℃	产物大小 Production size/bp
qSI-4	共显性 Codominant	4	RM8213	SSR	F: AGCCCAGTGATACAAAGATG R: GCGAGGAGATACCAAGAAAG	55	208
Bph14	共显性 Codominant	3	RI35	InDel	F: CAATCATCAAGCACGCGTTA R: ATCGAAGCCACTTGGTGAAC	58	325
Bph15	共显性 Codominant	4	Y15	InDel	F: AGGAACAGTGACACGTAGCA R: GGAGAGTTCAGTTTGCCATCC	55	460

株系 Line	抗虫基因 Insect-resistant gene			抗性均值 Average resistance	抗性水平 Resistance level
株系 Line	qsI-4	Bph14	Bph15	抗性均值 Average resistance	抗性水平 Resistance level
FJ717	+	+	+	2.93	R
FJ718	+	+	+	3.81	R
FJ719	-	+	+	3.08	R
FJ723	+	-	+	5.43	MR
FJ742	+	+	-	5.57	MR
FJ745	+	+	+	3.84	R
FJ747	-	-	+	6.67	S
B5	-	+	+	3.07	R
FH7011	+	-	-	6.33	S
FH676	-	-	-	8.38	S
TN1	-	-	-	7.87	S

株系 Line	抗虫基因 Insect-resistant gene			抗性均值 Average resistance	抗性水平 Resistance level
株系 Line	qsI-4	Bph14	Bph15	抗性均值 Average resistance	抗性水平 Resistance level
FJ717	+	+	+	2.93	R
FJ718	+	+	+	3.81	R
FJ719	-	+	+	3.08	R
FJ723	+	-	+	5.43	MR
FJ742	+	+	-	5.57	MR
FJ745	+	+	+	3.84	R
FJ747	-	-	+	6.67	S
B5	-	+	+	3.07	R
FH7011	+	-	-	6.33	S
FH676	-	-	-	8.38	S
TN1	-	-	-	7.87	S

品系 Line	株高 Plant height/cm	单株穗数 Panicle number per plant	每穗粒数 Grain number per panicle	每穗实粒数 Filled grain number per panicle	结实率 Seed-setting rate/%	千粒重 1000-grain weight/g
FH676(CK)	131.9±4.4	7.6±0.9	175.0±11.6	154.0±9.6	88.0±1.6	31.2±0.3
FJ717	127.4±1.2^*	8.3±1.2	173.6±13.6	159.0±12.8	91.6±1.9^*	30.7±0.3
FJ718	125.9±5.3^**	9.2±2.1^**	168.9±15.9^*	154.3±13.3	91.3±2.5^*	29.8±0.2
FJ719	133.9±6. 7	6.8±0.6^*	187.1±9.5^*	159.5±8.6	85.2±2.7^*	32.0±0.1
FJ745	126.9±5.2^**	7.4±1.3	169.5±12.3	151.4±10.2	89.3±0.4	30.1±0.4

Breeding Restore Lines of Hybrid Rice by Pyramiding Genes for Resistance to White Backed Planthoppers and Brown Planthoppers

聚合白背飞虱和褐飞虱抗性基因创制杂交水稻恢复系

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Figures/Tables 9

References 25

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杂交组合 Hybrid rice combination	单株穗数 Panicle number per plant	每穗粒数 Grain number per panicle	每穗实粒数 Filled grain number per panicle	结实率 Seed-setting rate/%	千粒重 1000-grain weight/g	理论产量 Theoretical yield /(kg∙hm^-2)
II-32A/FH676（CK）	9.7±0.9	190.5±13.6	177.6±9.6	93.2±1.4	28.2±0.9	9160.5±169.2
II-32A/FJ717	9.9±1.2	193.6±21.2	181.0±12.8	93.5±1.5	28.7±0.2	9749.3±210.2^**
II-32A/FJ718	10.1±2.1	188.3±5.6	175.7±13.3	93.3±2.0	28.1±1.1	9426.1±107.1^*
II-32A/FJ719	7.8±0.6	213.5±9.8	185.3±8.6	86.8±1.7	29.0±0.2	8878.3±192.5
II-32A/FJ745	10.4±1.3	194.5±18.4	173.8±10.2	89.3±1.3	27.2±0.4	9308.9±98.4
Y58S//FH676（CK）	9.1±0.8	205.1±11.6	176.4±9.6	86.0±0.9	26.2±0.3	7950.9±214.5
Y58S//FJ717	8.3±0.2	210.3±14.6	185.5±12.8	88.2±0.9	26.7±1.4	7732.2±110.2
Y58S//FJ718	10.3±1.4	196.7±5.6	171.5±13.3	87.2±3.2	26.6±0.1	8886.5±183.1^**
Y58S//FJ719	7.8±0.8	223.5±109.9	184.8±8.6	82.7±1.7	28.0±0.9	7571.3±199.2
Y58S//FJ745	9.8±0.3	197.4±13.2	174.2±10.2	88.3±1.4	26.1±0.3	8397.5±210.1^*

杂交组合 Hybrid rice combination	单株穗数 Panicle number per plant	每穗粒数 Grain number per panicle	每穗实粒数 Filled grain per panicle	结实率 Seed-setting rate/%	千粒重 1000-grain weight/g	理论产量 Theoretical yield /(kg∙hm^-2)
丰两优4号(CK) Fengliangyou 4(CK)	8.9±0.7	191.4±8.3	159.5±6.4	83.4±2.2	28.5±0.1	7619.2±203.5
Ⅱ优明86 ⅡYouming 86	9.3±0.7	190.7±4.4	174.0±3.3	91.2±2.8	28.3±0.1	8683.0±219.3
泰丰A/FJ717 Taifeng A/FJ717	10.3±0.5	186.6±11.3	161.4±8.6	86.5±1.7	25.3±0.1	7965.3±184.8^*
泰丰A/FJ718 Taifeng A/FJ718	9.8±0.4	179.5±10.3	157.2±9.0	87.6±3.4	25.0±0.1	7241.6±114.7
泰丰A/FJ745 Taifeng A/FJ745	10.3±0.4	182.8±13.4	157.9±12.0	86.4±1.8	25.3±0.1	7795.3±217.6
泸香618A/FJ717 Luxiang 618A/FJ717	9.7±0.3	199.8±9.1	178.4±7.3	89.3±1.2	25.4±0.2	8279.0±117.9^**
泸香618A/FJ718 Luxiang 618A/FJ718	11.1±0.7	176.4±9.8	154.1±6.3	87.3±0.8	25.5±0.2	8214.2±254.7^**
泸香618A/FJ745 Luxiang 618A/FJ745	9.4±1.0	193.0±12.7	171.8±10.7	89.0±1.1	25.8±0.4	7857.7±247.7

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