Correlation Between Genetic Distance Based on Molecular Markers of Functional Genes and Heterosis in Rice

doi:10.3969/j.issn.1001-7216.2009.06.02

Abstract

Abstract: The genetic difference among twentynine rice parents (eighteen cytoplasmic male sterile lines and eleven restorer lines) was analyzed by SSR molecular markers of functional genes related to yield traits. The correlation between genetic distance and heterosis was studied by reference to the performance of fortyseven combinations. The results showed that the correlation between the genetic distance and yield heterosis was significant(r=0.29*), indicating the possibility of forecasting heterosis according to the genetic distance based on molecular markers of functional genes. The correlation between the genetic distance and the heterosis of other yieldrelated traits was insignificant. However, the correlation coefficient obtained in the study was too small to predict heterosis.

Key words: rice, simple sequence repeats, genetic distance, heterosis, correlation

摘要： 利用与产量性状相关的功能基因标记分析了29个杂交水稻组合亲本间（18个不育系和11个恢复系）的遗传差异，结合29个亲本所配组合（47个）的F1表现，研究了功能基因标记遗传距离与杂种优势的相关性。结果表明，功能基因标记遗传距离与产量杂种优势呈显著正相关（r=0.29*），而与其他主要产量构成因素的杂种优势相关不显著。说明功能基因标记有可能应用于杂交水稻杂种优势预测，但得到的相关系数偏小，还不足以预测杂种优势。

关键词: 水稻, 微卫星标记, 遗传距离, 杂种优势

ZHANG Tao,#,NI Xian-lin,#,JIANG Kai-feng,YANG Qian-hua,YANG Li,WAN Xian-qi,CAO Ying-jiang,ZHENG Jia-kui, . Correlation Between Genetic Distance Based on Molecular Markers of Functional Genes and Heterosis in Rice[J]. Chinese Journal of Rice Science, DOI: 10.3969/j.issn.1001-7216.2009.06.02 .

张涛,倪先林, 蒋开锋杨乾华,杨莉万先齐,曹应江 ,郑家奎,. 水稻功能基因标记遗传距离与杂种优势的相关性研究[J]. 中国水稻科学, DOI: 10.3969/j.issn.1001-7216.2009.06.02 .

References

[1]朱国英，张为国. 杂交水稻苗期同工酶与杂种优势关系的研究. 作物学报, 1987, 13(21): 89-96.

[2]孙传清，姜廷波，陈亮，等. 水稻杂种优势与遗传分化关系的研究. 作物学报, 2000, 26(6): 641-649

[3]Hinze L L, Lamkey K R. Absence of epistasis for grain yield in elite maize hybrids. Crop Sci, 2003, 43: 46-56.

[4]Lee M, Godshalk K, Lamkey K R, et al. Association of restriction fragment length polymorphisms among maize inbreds with agronomic performance of their crosses. Crop Sci, 1989, 29(4): 1067-1071.

[5]Smith O S, Smith J S C, Bowen S L, et al. Similarities among a group of elite maize inbreds as measured by pedigree, F1 grain yield, grain yield,heterosis, and RFLPs. Theor Appl Genet, 1990, 80(6): 833-840.

[6]蔡健，兰伟. AFLP标记与水稻杂种产量及产量杂种优势的预测. 中国农学通报, 2005, 21(4): 39-43.

[7]赵庆勇, 朱镇，张亚东，等. SSR标记遗传距离与粳稻杂种优势的相关性分析. 中国水稻科学， 2009， 23（2）： 141-147

[8]张培江, 才宏伟, 李焕朝, 等. RAPD分子标记水稻遗传距离及其与杂种优势的关系. 安徽农业科学, 2000, 28(6): 697-700, 704.

[9]廖伏明，周坤炉，阳和华，等.杂交水稻亲本遗传差异及其与杂种优势关系. 中国水稻科学, 1998, 12(4): 193-199.

[10]张涛，韩磊，徐建第，等. 杂交香稻亲本遗传距离与产量杂种优势的相关性研究. 中国农业科学, 2006, 39(4): 831-835.

[11]Zhang Q F, Gao Y J, Yang S H, et al. A diallel analysis of heterosis in elite hybrid rice based on RFLPs and microsatellites. Theor Appl Genet, 1994, 9(2): 185-192.

[12]Zhang Q F, Gao Y J, Saghui Maroof M A, et al. Molecular divergence and hybrid performance in rice. Mol Breeding, 1995, 1(2): 133-142.

[13]Xiao J, Li J, Yuan L, et al. Genetic diversity and its relationship to hybrid performance and heterosis in rice as revealed by PCRbased markers. Theor Appl Genet, 1996, 92(6): 637-643.

[14]黄显波，田志宏，邓则勤，等. 水稻三明显性核不育基因的初步鉴定. 作物学报, 2008, 34(10): 1865-1868.

[15]Jiang L, Guo L B, Jiang H, et al. Genetic analysis and finemapping of a dwarfing with withered leaftip mutant in rice. J Genet Gen, 2008, 35(12): 715-721.

[16]McCouch S R, Teytelman L, Xu Y B, et al. Development and mapping of 2240 new SSR markers for rice (Oryza sativa L.). DNA Res, 2002, 9(6): 199-207.

[17]张涛. 水稻糙米蛋白质含量的QTL定位及香稻的资源研究[D]. 成都：四川农业大学，2007.

[18]Cho Y C, Suh J P, Choi I S, et al. QTLs analysis of yield and its related traits in wild rice relative Oryza rufipogon. Treat Crop Res, 2003, 4: 19-29.

[19]Xiao J, Grandillo S, Ahn S N, et al. Genes from wild rice improve yield. Nature, 1996, 384: 223-224.

[20]Xiao J H, Li J, Grandillo S, et al. Identification of traitimproving quantitative trait loci alleles from a wild rice relative, Oryza rufipogon. Genetics, 1998, 150: 899-909.

[21]Moncada P, Martinez C P, Borrero J, et al. Quantitative trait loci for yield and yield components in an Oryza sativa×Oryza rufipogon BC2F2 population evaluated in an upland environment. Theor Appl Genet, 2001, 102(1): 41-42.

[22]Thomson M J, Tai T H, McClung A M, et al. Mapping quantitative trait loci for yield, yield components and morphological traits in an advanced backcross population between Oryza rufipogon and the Oryza sativa cultivar Jefferson. Theor Appl Genet, 2003, 107: 479-493.

[23]Septiningsih E M, Prasetiyono J, Lubis E, et al. Identification of quantitative trait loci for yield and yield components in an advanced backcross population derived from the Oryza sativa variety IR64 and the wild relative O. rufipogon. Theor Appl Genet, 2003, 107: 1419-1432.

[24]Song X J, Huang W, Shi M, et al. A QTL for rice grain width and weight encodes a previously unknown RINGtype E3 ubiquitin ligase. Nat Genet, 2007, 39(5): 623-630.

[25]Marri P R, Sarla N, Reddy L V, et al. Identification and mapping of yield and yield related QTLs from an Indian accession of Oryza rufipogon. BMC Genetics, 2005, 6(33): 114.

[26]邓其明. 水稻寡分蘖候选基因的定位、克隆及水稻白叶枯病抗性分子标记辅助育种[D]. 成都：四川农业大学, 2005.

[27]Fan C C, Xing Y Z, Mao H L, et al. GS3, a major QTL for grain length and weight and minor QTL for grain width and thickness in rice, encodes a putative transmembrane protein. Theor Appl Genet, 2006, 112(6): 1164-1171.

[28]Ayahiko S, Takeshi I, Kaworu E, et al. Deletion in a gene associated with grain size increased yields during rice domestication. Nat Genet, 2008, 40(8): 1023-1028.

[29]Tian F, Li D J, Fu Q, et al. Construction of introgression lines carrying wild rice (Oryza rufipogon Griff.) segments in cultivated rice (Oryza sativa L.) background and characterization of introgressed segments associated with yieldrelated traits. Theor Appl Genet, 2006，112(3): 570-580.

[30]Li X Y, Qian Q, Fu Z M, et al. Control of tillering in rice. Nature, 2003, 422: 618-621.

[31]Xue W Y, Xing Y Z, Weng X Y, et al. Natural variation in Ghd7 is an important regulator of heading date and yield potential in rice. Nat Genet, 2008, 40(6): 761-767.

[32]Hua J P, Xing Y Z, Wu W R, et al. Singlelocus heterotic effects and dominance by dominance interactions can adequately explain the genetic basis of heterosis in an elite rice hybrid. Proc Natl Acad Sci USA, 2003, 100: 2574-2579.

[33]Li X H, Xu C G, Gao Y J, et al. Analyzing quantitative trait loci for yield using a vegetatively replicated F2 population from a cross between the parents of an elite rice hybrid. Theor Appl Genet, 2000, 101: 248-254.

[34]Nei M, Li W. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci USA, 1979, 76(10): 5269-5273.

[35]赵勇，杨凯，Cheema A A，等. 利用水稻功能基因SSR标记鉴定水稻种质资源. 中国农业科学, 2002, 35(4): 349-353.

[36]李云海，钱前，曾大力，等. 我国主要杂交水稻亲本的RAPD鉴定及遗传关系研究. 作物学报, 2000, 26(2): 171-176.

[37]Wang G J, Castiglione S, Zhang J，et al. Hybrid rice (Oryza sativa L.): Identification and parentage determination by RAPD fingerprinting. Plant Cell Rep, 1994, 14: 112-115.

[38]朱作峰, 孙传清, 王象坤，等. 水稻品种SSR与RFLP及其与杂种优势的关系比较研究.遗传学报, 2001, 28(8): 738-745.

[39]李任华, 徐才国, 何予卿, 等. 水稻亲本遗传分化程度与籼粳杂种优势的关系. 作物学报, 1998, 24(5): 564-576.

[40]刘冬成, 张爱民. 作物杂种优势基础研究的进展. 中国科学院院刊, 2001(5): 334-338.

[41]Zhang Q F, Zhou Z Q, Yang G P, et al. Molecular marker heterozygosity and hybrid performance in indica and japonica rice. Theor Appl Genet, 1996, 93(8): 1218-1224.

[42]Zhao M F, Li X H, Yang J B, et al. Relationship between molecular marker heterozygosis and hybrid performance in intra and inter-subspecific crosses of rice. Plant Breeding, 1999, 18(2): 139-144.