Chinese Journal of Rice Science

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Cloning and Expression Analysis of a Calmodulin-Binding Protein Gene from Rice

WAN Jia; KUANG Hao-chi; XIE Bi-wu; CHEN Jing; ZHANG Qi; XU Yong-ju; GAO Xiao-ling; XU Zhen-jun*   

  • Received:1900-01-01 Revised:1900-01-01 Online:2008-05-10 Published:2008-05-10

一个水稻钙调素结合蛋白基因OsCaMBP的克隆及表达分析

万佳1,#;况浩池2,#;谢必武3,#;陈静4;张琦1;徐永菊1;高晓玲1,5;徐正君1,5,*   

  1. 1四川农业大学 水稻研究所, 四川 温江 611130; 2四川省农业科学院 水稻高粱研究所, 四川 泸州 646100; 3重庆三峡职业学院 生物工程系, 重庆 万州 404001; 4四川绵阳金地高科种业有限公司, 四川 绵阳 621000; 5四川农业大学 作物基因资源与遗传改良教育部重点实验室, 四川 雅安 625014; #共同第一作者; *通讯联系人, E-mail:mywildrice@yahoo.com.cn

Abstract: By using fluorescence differential display and RT-PCR methods, a new member of the calmodulin-binding proteins family of rice was isolated and termed as OsCaMBP.The full length cDNA was 2094 bp and encoded a polypeptide containing 569 amino acid residues (63.2 kD). Amino acid sequence of the deduced protein revealed the existence of IQ calmodulin-binding motif at the N-terminus. Similarity analysis indicated that rice OsCaMBP has 38.25%-47.28% identities with CaMBPs in other plants. Expression of the OsCaMBP gene was greatly increased in leaves, roots and leaf sheaths after the heat shock treatment at 42℃ for 15min, 30min, 1h and 2h. Furthermore, the gene expression accumulation was enhanced in response to chilling stress.

Key words: rice, calmodulin-binding protein, fluorescence differential display, gene cloning, expression

摘要: 通过荧光差异显示和RTPCR技术,克隆到一个新的水稻钙调素结合蛋白基因OsCaMBP。序列分析表明,该基因cDNA序列全长2094 bp,编码一个具有569个氨基酸残基的多肽,推测分子量为63.2 kD;在OsCaMBP蛋白的N端存在IQ钙调素结合构象,与其他植物中的钙调素结合蛋白的相似性介于38.25%~47.28%。在热激处理15 min、30 min、 1 h或2 h后,该基因在水稻的叶、根和叶鞘中表达量急剧增加;此外,该基因表达量也受低温调控而增加。

关键词: 水稻, 钙调素结合蛋白, 荧光差异显示, 基因克隆, 表达